Presumptive Mediators of Growth Hormone Action on Insulin-Like Growth Factor I Release by Porcine Ovarian Granulosa Cells

Neurosignals ◽  
2000 ◽  
Vol 9 (5) ◽  
pp. 248-254 ◽  
Author(s):  
A.V. Makarevich ◽  
A.V. Sirotkin
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Granulosa Cells ◽  
Insulin Like Growth Factor ◽  
Hormone Action ◽  
Factor I ◽  
Ovarian Granulosa Cells ◽  
Growth Hormone Action ◽  
Growth Factor I

scholarly journals The Effect of Capsaicin on IGF-I and IGF-IR Expression in Ovarian Granulosa Cells

2020 ◽  
Vol 71 (1) ◽  
pp. 1977
Author(s):  
S. GULER ◽  
Β. ZIK
Keyword(s):  
Growth Factor ◽  
Granulosa Cells ◽  
Ovarian Development ◽  
Ovary Development ◽  
Insulin Like Growth Factor ◽  
Sprague Dawley ◽  
Factor I ◽  
Ovarian Granulosa Cells ◽  
Igf I ◽  
Growth Factor I

Capsaicin (trans-8-methyl-N-vanillyl-6-noneadamide) is a pungent ingredient in red peppers from the Capsicum family. Insulin-like growth factor-I (IGF-I) is expressed in granulosa cells and has an important role in ovarian development. However, there are no data about the IGF-I expression in ovarian granulosa cells after capsaicin treatment. The aim of this study was to investigate the expression of IGF-I and its receptor (insulin-like growth factor-I receptor [IGF-IR]) in primary rat ovarian granulosa cells after low and high doses of capsaicin treatment. For this, granulosa cells were isolated and cultured from ovaries of 30-day-old female Sprague-Dawley rats. Granulosa cell plates were divided into four groups as cell control (C), vehicle control (V), and 50 μM and 150 μM capsaicin groups. In experimental groups, granulosa cells were exposed to capsaicin for 24 hours and immunocytochemistry was performed afterwards using anti-IGF-I and anti-IGF-IR antibodies. Both IGF-I and IGF-IR expressions were found to be significantly increased in parallel to the capsaicin doses. Elevated levels of IGF-I may be a risk factor for ovarian development. Because of the crucial role of IGF-I in ovary development, capsaicin treatment can be effective on follicular development and/or disorders characterized by high IGF-I levels.

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