Placental Transfer of Pyrimethamine Studied in an ex vivo Placental Perfusion Model

Neonatology ◽  
2000 ◽  
Vol 78 (2) ◽  
pp. 83-85 ◽  
Author(s):  
Gilles Peytavin ◽  
Jean-Joël Leng ◽  
François Forestier ◽  
Marie-Claude Saux ◽  
Patrick Hohlfeld ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

Placental transfer of bromocriptine in an ex vivo human placental perfusion model

2017 ◽  
Vol 32 (7) ◽  
pp. 1155-1159 ◽  
Author(s):  
Qiaoling Zheng ◽  
Qiong Zhou ◽  
Juan Li ◽  
Yuqin Tian ◽  
Hua Huang ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model

Placental transfer of rosiglitazone in the ex vivo human perfusion model

2006 ◽  
Vol 195 (6) ◽  
pp. 1715-1719 ◽  
Author(s):  
Heather J. Holmes ◽  
Brian M. Casey ◽  
Roger E. Bawdon
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Perfusion Model

scholarly journals Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System

Planta Medica ◽  
2021 ◽  
Author(s):  
Deborah Spiess ◽  
Vanessa Fabienne Abegg ◽  
Antoine Chauveau ◽  
Andrea Treyer ◽  
Michael Reinehr ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Lactate Production ◽  
Glucose Consumption ◽  
Positive Control ◽  
Placental Perfusion ◽  
Perfusion Model ◽  
Beta Human Chorionic Gonadotropin ◽  
Pathologic Findings ◽  
Age Appropriate ◽  
Placental Passage

AbstractThe placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings.

scholarly journals Plasma proteins facilitates placental transfer of polystyrene particles

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Michael M. Gruber ◽  
Birgit Hirschmugl ◽  
Natascha Berger ◽  
Magdalena Holter ◽  
Snježana Radulović ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Placenta ◽  
Plasma Proteins ◽  
Placental Transfer ◽  
Ex Vivo ◽  
Biological Fluids ◽  
Protein Corona ◽  
Human Albumin ◽  
Placental Perfusion ◽  
The Impact

Abstract Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications.

Ex Vivo Human Placental Perfusion Model for Analysis of Fetal Circulation in the Chorionic Plate

2016 ◽  
Vol 35 (3) ◽  
pp. 553-560 ◽  
Author(s):  
Zoya Gordon ◽  
Lilia Glaubach ◽  
David Elad ◽  
Uri Zaretsky ◽  
Ariel J. Jaffa
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Fetal Circulation ◽  
Perfusion Model ◽  
Chorionic Plate

Utilization of an ex vivo human placental perfusion model to predict potential fetal exposure to carboplatin during pregnancy

2014 ◽  
Vol 210 (3) ◽  
pp. 275.e1-275.e9 ◽  
Author(s):  
Judith A. Smith ◽  
Anjali Gaikwad ◽  
Scott Mosley ◽  
Larry Coffer ◽  
Jeffrey Cegelski ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Fetal Exposure ◽  
Placental Perfusion ◽  
Perfusion Model

Placental transfer of sulindac, sulindac sulfide, and indomethacin in a human placental perfusion model

1999 ◽  
Vol 180 (1) ◽  
pp. 174-180 ◽  
Author(s):  
Eija S. Lampela ◽  
Laura H. Nuutinen ◽  
Tero I. Ala-Kokko ◽  
Riitta M. Parikka ◽  
Risto S. Laitinen ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Placental Perfusion ◽  
Perfusion Model ◽  
Sulindac Sulfide

Placental transfer of lopinavir/ritonavir in the ex vivo human cotyledon perfusion model

2006 ◽  
Vol 195 (1) ◽  
pp. 296-301 ◽  
Author(s):  
Laurent Gavard ◽  
Sophie Gil ◽  
Gilles Peytavin ◽  
Pierre-François Ceccaldi ◽  
Claudia Ferreira ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Ex Vivo ◽  
Perfusion Model

Ex vivo human placental perfusion model for analysis of feto-placental vasculature

Placenta ◽  
2017 ◽  
Vol 57 ◽  
pp. 272 ◽  
Author(s):  
David Elad ◽  
Uri Zaretsky ◽  
Zoya Gordon ◽  
Ariel Jaffa
Keyword(s):  
Ex Vivo ◽  
Placental Perfusion ◽  
Perfusion Model
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