Protein Tyrosine Kinase Inhibitors Block the Stimulatory Actions of Phosphotyrosine Phosphatase Inhibitors to Increase Cell Proliferation, Alkaline Phosphatase Activity, and Collagen Synthesis in Normal HumanBone Cells

2000 ◽  
Vol 20 (2) ◽  
pp. 153-162 ◽  
Author(s):  
Hyun Koo Yoon ◽  
David J. Baylink ◽  
K.-H. William Lau
Keyword(s):  
Cell Proliferation ◽  
Alkaline Phosphatase ◽  
Tyrosine Kinase ◽  
Phosphatase Activity ◽  
Tyrosine Kinase Inhibitors ◽  
Alkaline Phosphatase Activity ◽  
Protein Tyrosine Kinase ◽  
Collagen Synthesis ◽  
Kinase Inhibitors ◽  
Phosphatase Inhibitors

Opposing effects of protein tyrosine kinase inhibitors on the monoclonal antibody induced internalization of CD3 and CD4 antigens

1991 ◽  
Vol 21 (10) ◽  
pp. 2641-2643 ◽  
Author(s):  
Laure Thuillier ◽  
Jean-Louis Pérignon ◽  
Françoise Selz ◽  
Claude Griscelli ◽  
Alain Fischer
Keyword(s):  
Monoclonal Antibody ◽  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitors ◽  
Protein Tyrosine Kinase ◽  
Kinase Inhibitors ◽  
Protein Tyrosine ◽  
Opposing Effects

Acidosis inhibits osteoblastic and stimulates osteoclastic activity in vitro

1992 ◽  
Vol 262 (3) ◽  
pp. F442-F448 ◽  
Author(s):  
N. S. Krieger ◽  
N. E. Sessler ◽  
D. A. Bushinsky
Keyword(s):  
Alkaline Phosphatase ◽  
Metabolic Acidosis ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Collagen Synthesis ◽  
Calcium Flux ◽  
Osteoclastic Activity ◽  
Glucuronidase Activity

Metabolic acidosis induces net calcium flux (JCa) from cultured neonatal mouse calvariae through physicochemical and cell-mediated mechanisms. To determine the role of osteoblasts in acid-induced JCa, collagen synthesis and alkaline phosphatase activity were assessed in calvariae incubated in reduced pH and bicarbonate medium, a model of metabolic acidosis (Met), and compared with controls (Ctl). Collagen synthesis fell from 30.5 +/- 1.1 in Ctl to 25.1 +/- 0.4% with Met, and alkaline phosphatase decreased from 403 +/- 25 in Ctl to 298 +/- 21 nmol Pi.min-1.mg protein-1 with Met. During acidosis JCa was correlated inversely with percent collagen synthesis (r = -0.743, n = 11, P = 0.009) and with alkaline phosphatase activity (r = -0.453, n = 22, P = 0.034). To determine the role of osteoclasts in acid-induced JCa, osteoclastic beta-glucuronidase activity was determined in Ctl and Met in the absence or presence of the osteoclastic inhibitor calcitonin (CT, 3 x 10(-9) M). Met increased beta-glucuronidase (5.9 +/- 0.2) compared with Ctl (4.6 +/- 0.3 micrograms phenolphthalein released.bone-1.h-1), whereas CT inhibited beta-glucuronidase in both Ctl and Met (3.1 +/- 0.2 and 3.5 +/- 0.3, respectively). During acidosis JCa was correlated directly with beta-glucuronidase activity (r = 0.683, n = 42, P less than 0.001). Thus the cell-mediated component of JCa during acidosis in vitro appears to result from a combination of inhibited osteoblastic and stimulated osteoclastic activity.

6 Protein Tyrosine Kinase Inhibitors

1996 ◽  
pp. 233-329 ◽  
Author(s):  
Paul W. Groundwater ◽  
Kevin R.H. Solomons ◽  
Jacqueline A. Drewe ◽  
Munawar Ali Munawar
Keyword(s):  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitors ◽  
Protein Tyrosine Kinase ◽  
Kinase Inhibitors ◽  
Protein Tyrosine
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