Water Extracts of Helicobacter pylori Suppress the Expression of Histidine Decarboxylase and Reduce Histamine Content in the Rat Gastric Mucosa

Digestion ◽  
2000 ◽  
Vol 62 (2-3) ◽  
pp. 100-109 ◽  
Author(s):  
Peter C. Konturek ◽  
Tomasz Brzozowski ◽  
Elżbieta Karczewska ◽  
Aleksandra Duda ◽  
Władysław Bielański ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Histidine Decarboxylase ◽  
Histamine Content ◽  
Water Extracts ◽  
Rat Gastric Mucosa

scholarly journals Isolation and properties of multiple forms of histidine decarboxylase from rat gastric mucosa

1982 ◽  
Vol 205 (2) ◽  
pp. 405-412 ◽  
Author(s):  
A Savany ◽  
L Cronenberger
Keyword(s):  
Gastric Mucosa ◽  
Isoelectric Focusing ◽  
Histidine Decarboxylase ◽  
Specific Activity ◽  
Proteinase Inhibitors ◽  
Gel Chromatography ◽  
High Loss ◽  
Carrier Ampholytes ◽  
Km Value ◽  
Rat Gastric Mucosa

The heterogeneity of histidine decarboxylase from rat gastric mucosa was studied. The partially purified enzyme was fractionated by preparative isoelectric focusing on a flat-gel bed by using narrow pH-range carrier ampholytes and a short focusing time. The activity was resolved, with about 95% recovery, into three forms, designated I, II and III, with pI values of 5.90, 5.60 and 5.35 respectively. These three forms exhibited similar molecular weights, indicating that the forms were not the result of different degrees of polymerization. By preparative refocusing each form refocused as a single peak of enzyme activity with reproducible pI, but a high loss of activity occurred with repeated focusing. Forms I, II and III were purified by the combined use of preparative isoelectric focusing and gel chromatography and other fractionation methods. The active forms could be distinguished by electrophoresis and isoelectric focusing on polyacrylamide gels and displayed protein heterogeneity. These forms were found in the crude extract and in the partially purified preparations in the presence or absence of proteinase inhibitors. Form II had the highest specific activity, but all three forms had the same optimum pH and Km value for histidine.

scholarly journals Electron microscopic identification of histidine decarboxylase-containing endocrine cells of the rat gastric mucosa

1984 ◽  
Vol 87 (3) ◽  
pp. 496-502 ◽  
Author(s):  
Hiroaki Kubota ◽  
Yoshitaka Taguchi ◽  
Masaya Tohyama ◽  
Nariaki Matsuura ◽  
Sadao Shiosaka ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Endocrine Cells ◽  
Histidine Decarboxylase ◽  
Electron Microscopic ◽  
Microscopic Identification ◽  
Rat Gastric Mucosa ◽  
Electron Microscopic Identification
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