scholarly journals Impaired Relaxation in Aorta from Streptozotocin-diabetic Rats: Effect of Aminoguanidine (AMNG) Treatment

2000 ◽  
Vol 1 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Sibel Özyazgan ◽  
Yesim Unlucerci ◽  
Selda Bekpinar ◽  
Ahmet Gökhan Akkan
Keyword(s):  
Drinking Water ◽  
Product Formation ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Vasoactive Substances ◽  
Blood Glucose Levels ◽  
Body Weights ◽  
Endothelium Dependent Relaxation ◽  
Advanced Glycosylation

AimThe effect of 8 weeks′ streptozotocin (STZ)- induced diabetes and aminoguanidine (AMNG), the inhibitor of advanced glycosylation reaction, treatment on arteriolar reactivity to vasoactive substances was investigatedin vitro.Materials and MethodsStudies were performed in untreated control rats (n= 10), STZ-induced (60 mg/kg i.v.) diabetic rats (n= 10), AMNG-treated (600 mg/l given in drinking water throughout 8 weeks) control rats (n= 10) and AMNG-treated (600 mg/l given in drinking water, beginning at 72h after STZ and throughout 8 weeks of diabetes) diabetic rats (n= 10). Results are expressed as the mean ±s.e. Relaxant responses are expressed as a percentage (%) relaxation of noradrenaline-induced tone. Statistical comparisons were made by one-way analysis of variance (ANOVA) followed by Tukey–Kramer multiple comparisons test.Results1. The decreased body weights (205 ± 6 g) and increased blood glucose levels (583 ± 8 mg/dl) of diabetic rats were partially restored by treatment of aminoguanidine (253 ± 6 g,p< 0.05 and 480 ± 14 mg/dl,p< 0.001, respectively). 2. Diabetes caused a 71% deficit in maximal endothelium-dependent relaxation to acetylcholine for noradrenaline precontracted aortas (p< 0.001). AMNG treatment prevented the diabetes-induced impairment in endothelium dependent relaxation (58 ± 8%) to acetylcholine, maximum relaxation remaining in the non-diabetic range (78 ± 4%). 3. Neither diabetes nor treatment affected endothelium-independent relaxation (pD2and max. Relax.) to sodium nitroprusside. 4. Vasoconstrictor responses (pD2and Max. Contraction) to noradrenaline and KCl were not influenced by the diabetic state and treatment.ConclusionOur data suggest that 8 weeks of experimental diabetes is associated with a decreased endothelium-dependent vasodilatation. AMNG treatment may prevent diabetes-induced endothelial dysfunction. This may be mediatedviathe prevention of advanced glycosylation end product formation, the enhanced release of vasodilator substances such as prostacyclin, the increased elasticity of blood vessels, the antioxidant activity and inhibitor activity of enzyme aldose-reductase by AMNG.

Chenopodium quinoa Exhibits Antihyperglycemic Activity in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Vol 19 ◽  
Author(s):  
Amine Azzane ◽  
Ayou Amssayef ◽  
Mohame Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Chenopodium Quinoa ◽  
Diabetic Rats ◽  
Favorable Effect ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The aim of the study was to evaluate the antihyperglycemic effect of Chenopodium quinoa. Background: Chenopodium quinoa is a pseudocereal plant with several medicinal properties. Objective: The goal of this investigation was to determine the antihyperglycemic activity of Chenopodium quinoa in both normal and streptozotocin(STZ)-induced diabetic rats. Methods: In this study, the effect of the aqueous extract of Chenopodium quinoa seeds (AECQS) (60 mg/kg) on blood glucose levels was evaluated in both normal and diabetic rats after a single (6 hours) and repeated oral administration (7 days of treatment). The effect of this herb on glucose tolerance and lipid profile was also studied. Additionally, histopathological examination of liver was carried out using the Hematoxylin-Eosin method. Furthermore, the in vitro antioxidant activity as well as a preliminary phytochemical screening and quantification of some secondary metabolites (phenolic compounds, flavonoids and tannins) were performed according to standard methods. Results: AECQS produced a significant lowering effect on plasma glucose levels in STZ-induced diabetic rats. In addition, this extract exhibited a remarkable amelioration on hepatic histopathology in diabetic rats. In addition, the extract exerted a remarkable antioxidant activity which could be due to the presence of some compounds found in this herb. Conclusion: In conclusion, this study demonstrates that the aqueous extract of Chenopodium quinoa seeds has a favorable effect in controlling diabetes mellitus.

Antihyperglycemic potential of Matricaria pubescens(Desf.) Schultz. in streptozotocin-induced diabetic rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohammed Ajebli ◽  
Mohamed Eddouks
Keyword(s):  
Aqueous Extract ◽  
Aerial Part ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Liver And Pancreas ◽  
Treatment Of Diabetes ◽  
Antioxidant Effects

Aims: The aim of the study was to investigate the antihyperglycemic activity of Matricaria pubescens. Background: Matricaria pubescens (Def). Shultz (Asteraceae) is commonly used traditionally for the treatment of diabetes in Morocco. Objective: This present investigation aimed to assess the antihyperglycemic and antioxidant effects of the aqueous extract of aerial part of Matricaria pubescens (M. pubescens). Methods: The effect of a single and repeated oral administration of the aqueous extract of aerial part of M. pubescens (AEAPMP) at a dose of 40 mg/kg on glucose was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of pancreas and liver was carried out according to the Hematoxylin-Eosin method. The antioxidant activity was performed using the DPPH assay. Results: The results showed that the aqueous extract of M. pubescens (AEAPMP) exhibited a significant lowering activity on blood glucose levels in STZ-induced diabetic rats. In addition, AEAPMP ameliorated the histopathological tissues of liver and pancreas. Furthermore, a potential in vitro antioxidant of AEAPMP has been shown. Conclusion: In conclusion, this study demonstrates that M. pubescens possesses a beneficial effect against hyperglycemia associated with diabetes.

scholarly journals Evaluation ofMallotus oppositifoliusMethanol Leaf Extract on the Glycaemia and Lipid Peroxidation in Alloxan-Induced Diabetic Rats: A Preliminary Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
C. O. Nwaehujor ◽  
I. I. Ezeigbo ◽  
F. C. Nwinyi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Treatment ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective. Mallotus oppositifolius(Geiseler) Müll. Arg. (Euphorbiaceae) is folklorically used to “treat” diabetic conditions in some parts of Nigeria therefore the study, to investigate the extract of the leaves for activities on hyperglycaemia, lipid peroxidation, and increased cholesterol levelsin vivoin alloxan diabetic rats as well as its potential antioxidant activityin vitro.Methods. Albino rats (240–280 g) were given an injection of 120 mg/kg body weight, i.p. of alloxan monohydrate. After 8 days, diabetic animals with elevated fasting blood glucose levels (>9 mmol/L) were considered and selected for the study.Results. Oral treatment with the extract administered every 12 h by gavage at doses of 100, 200, and 400 mg/kg of the extract to the test rats, for 14 days, resulted in a significant dose-dependent decrease in blood glucose levels from 12.82 ± 1.02 mmol/dL to 4.92 ± 2.01 mmol/dL at the highest dose of 400 mg/kg compared to the control drug and glibenclamide as well as attendant significant decline in diabetic rats employed in the study.Conclusion. The extract also showedin vitroconcentration-dependent antioxidant activity following the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing assays. Findings further suggest the presence of active antidiabetic and antioxidant principles inM. oppositifoliusleaves.

scholarly journals Decrease of Klotho in the Kidney of Streptozotocin-Induced Diabetic Rats

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Meng-Fu Cheng ◽  
Li-Jen Chen ◽  
Juei-Tang Cheng
Keyword(s):  
Oxidative Stress ◽  
High Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Renal Functions ◽  
Blood Glucose Levels ◽  
Convoluted Tubules ◽  
The Brain ◽  
Darby Canine Kidney

Theklothogene is expressed in a limited number of tissues, most notably in distal convoluted tubules in the kidney and choroid plexus in the brain. A previous study suggested that Klotho increases resistance to oxidative stress. However, changes of Klotho expression in high glucose-induced oxidative stress remain unclear. In the present study, we used streptozotocin-induced diabetic rats (STZ rats) to examine the effects of insulin, phloridzin or antioxidant, tiron on diabetic nephropathy. Both insulin and phloridzin reversed the lower Klotho expression levels in kidneys of STZ rats by the correction of hyperglycemia. Also, renal functions were improved by these treatments. In addition to the improvement of renal functions, the decrease of Klotho expression in kidney of STZ rats was also reversed by tiron without changing blood glucose levels. The reduction of oxidative stress induced by high glucose can be considered for this action of tiron. This view was further confirmed in vitro using high glucose-exposed Madin-Darby canine kidney (MDCK) epithelial cells. Thus, we suggest that decrease of oxidative stress is not only responsible for the improvement of renal function but also for the recovery of Klotho expression in kidney of STZ rats.

Therapeutic insights of hydromehtanolic leaf extract of Xanthium indi-cum and α-tocopherol in streptozotocin induced diabetic renal oxidative stress

2017 ◽  
Vol 6 (5) ◽  
pp. 158-166
Author(s):  
Anila Devi Meruva ◽  
Ravi Sahukari ◽  
Venkata Subbaiah Ganjikunta ◽  
Shanmugam Bhasha ◽  
Sathyavelu Reddy Kesireddy
Keyword(s):  
Blood Glucose ◽  
Serum Insulin ◽  
Microvascular Complications ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Body Weights ◽  
Mda Content

Diabetic nephropathy (DN) is one of the important microvascular complications of diabetes mellitus. Several phytochemicals have been reported to show beneficial consequences in Xanthium indicum leaves and α-tocopherol is a well known antioxidant, putting a halt to the fatality of renal dysfunc-tions in DN. In the current study, seven groups of male albino rats six in each group, received the following treatment scheduled for 4 weeks: Normal control, Xanthium indicum, α-tocopherol, Diabetes control, Glibenclamaide treated diabetic, α-tocopherol treated diabetic and Xanthium treated diabetic. Evaluations were made for blood glucose levels, body weights, serum insulin levels, MDA content, creatintne in urine as well as in serum and the histopathological changes were monitored kidney tissues in all experimental rats. Blood glucose levels were significantly (***P<0.001) decreased whereas serum insulin levels and body weights were significantly (***P< 0.001) in-creased, MDA content, serum creatinine levels were significantly (***P< 0.01), (***P<0.001), decreased and urine creatinine levels were increased with the treatment of plant extract and α-tocopherol in diabetic rats. Overall, the findings of this study indicated that the hydromethanolic extract of X. indicum leaves and α-tocopherol possesses a potent capacity that attenuates the renal damage to minimize the deleterious effects of free radicals by maintaining renal hemodynamics in diabetic conditions probably through its antioxidative and hypoglycaemic activity.

Aqueous Extract of Brassica rapa Exerts Antihyperglycemic Activity in Streptozotocin-induced Diabetic Rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Ismail Bouadid ◽  
Ayoub Amssayef ◽  
Nadia Lahrach ◽  
Ahmed El-Haidani ◽  
Mohamed Eddouks
Keyword(s):  
Brassica Rapa ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Lipid Profiles ◽  
Antioxidant Effect ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The aim of the study was to assess the antihyperglycemic effect of Brassica rapa. Background: Brassica rapa (turnip) is used as an antidiabetic plant. Objective: This work aimed to evaluate the effect of the aqueous extract of Brassica rapa seeds (AEBRS) on glycemia in vivo. Methods: The effect of AEBRS (60 mg/kg) on glycemia and lipid profiles was evaluated. Besides, preliminary phytochemical analysis and the in vitro antioxidant effect were evaluated. Results: AEBRS caused a significant reduction in blood glucose levels in diabetic rats (p<0.0001). In contrast, no significant effect was observed on lipid profiles, whereas antioxidant potential of this extract has been shown. Phytochemical analysis showed the presence of many important phytochemical families. Conclusion: The present study shows that AEBRS has a potent antihyperglycemic ability in diabetic rats.

Modulation of GLUT4 expression by oral administration of Mg2+ to control sugar levels in STZ-induced diabetic rats

2014 ◽  
Vol 92 (6) ◽  
pp. 438-444 ◽  
Author(s):  
Haniah Solaimani ◽  
Nepton Soltani ◽  
Kianoosh MaleKzadeh ◽  
Shahla Sohrabipour ◽  
Nina Zhang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Magnesium Sulfate ◽  
Mrna Expression ◽  
Insulin Level ◽  
Diabetic Rats ◽  
In Vivo Studies ◽  
Glucose Levels ◽  
Blood Glucose Levels

It has been previously shown that oral magnesium administration decreases the levels of glucose in the plasma. However, the mechanisms are not fully understood. The aim of this study was to determine the potential role of GLUT4 on plasma glucose levels by orally administering magnesium sulfate to diabetic rats. Animals were distributed among 4 groups (n = 10 rats per group): one group served as the non-diabetic control, while the other groups had diabetes induced by streptozotocin (intraperitoneal (i.p.) injection). The diabetic rats were either given insulin by i.p. injection (2.5 U·(kg body mass)–1·day–1), or magnesium sulfate in their drinking water (10 g·L–1). After 8 weeks of treatment, we conducted an i.p. glucose tolerance test (IPGTT), measured blood glucose and plasma magnesium levels, and performed in-vitro and in-vivo insulin level measurements by radioimmunoassay. Gastrocnemius (leg) muscles were isolated for the measurement of GLU4 mRNA expression using real-time PCR. Administration of magnesium sulfate improved IPGTT and lowered blood glucose levels almost to the normal range. However, the insulin levels were not changed in either of the in-vitro or in-vivo studies. The expression of GLU4 mRNA increased 23% and 10% in diabetic magnesium-treated and insulin-treated groups, respectively. Our findings suggest that magnesium lowers blood glucose levels via increased GLU4 mRNA expression, independent to insulin secretion.

Glucose Lowering Activity of Aqueous Ammodaucus leucotrichus Extract in Diabetic Rats

2020 ◽  
Vol 20 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Fadwa El-Ouady ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Medicinal Plant ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Traditional Use ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Ammodaucus leucotrichus (Apiaceae) is a medicinal plant widely used by the Moroccan population to treat various diseases including diabetes. Aims: The aim of this study was to evaluate the antidiabetic effect of the aqueous extract prepared from the fruits of this medicinal plant. Normal and streptozotocin-induced diabetic rats were treated orally by the extract of Ammodaucus leucotrichus (A. leucotrichus) at a dose of 10 mg/kg. Methods: The blood glucose levels were determined during 6 hours and 15 days during this treatment. In addition the oral glucose tolerance test as well as the analysis of histopathological changes of liver was performed. The antioxidant activity of aqueous A. Leucotrichus extract was also valuated by the method of trapping of free radical 2,2-diphenyl-1 picrylhydrazyl (DPPH). Results: A. Leucotrichus fruit aqueous extract (ALFAE) reduced the blood glucose levels in both normal and diabetic rats. In addition, a remarkable influence on glucose tolerance was also noticed after ALFAE treatment. Moreover, ALFAE was able to exhibit a beneficial effect on histologlogical structure of liver. Finally a potential antioxidant effect has been shown in vitro. Conclusion: In conclusion, this study demonstrates the antihyperglycemic and hypoglycemic effects of A. Leucotrichus in rats supporting then its traditional use for the management of diabetes.

scholarly journals Core structure of flavonoids precursor as an antihyperglycemic and antihyperlipidemic agent: an in vivo study in rats.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Mahmoud Najafian ◽  
Azadeh Ebrahim-Habibi ◽  
Parichehreh Yaghmaei ◽  
Kazem Parivar ◽  
Bagher Larijani
Keyword(s):  
Beneficial Effect ◽  
Therapeutic Potential ◽  
Urine Volume ◽  
Diabetic Rats ◽  
Core Structure ◽  
Alpha Amylase ◽  
Glucose Levels ◽  
Blood Glucose Levels

trans-Chalcone is the core structure of naringenin chalcone, located halfway in the biosynthesis pathway of flavonoids. Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and related disorders. As a mammalian alpha-amylase inhibitor in vitro, the putative beneficial effect of trans-chalcone on diabetes was tested in a streptozotocin-induced rat model of diabetes type 1, and the results analyzed with commonly used statistical methods. Significant reduction of blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. trans-chalcone was observed to have a weight loss-inductive effect, alongside with a reduction in food intake, which is suggestive of a therapeutic potential of this compound in overweight and obese patients.

scholarly journals Hypoglycemic and Antioxidant Effects of Subcoriacin in Normal and Streptozotocin-induced Diabetic Rats

2019 ◽  
Vol 54 (4) ◽  
Author(s):  
José M. Narváez-Mastache ◽  
Claudia Soto ◽  
Guillermo Delgado
Keyword(s):  
Antioxidant Enzymes ◽  
Blood Glucose ◽  
Antioxidant Activities ◽  
Combined Treatment ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Endogenous Antioxidant

Subcoriacin (1) is a 3-aryl-6-prenylcoumarin isolated from Eysenhardtia subcoriacea that has shown antioxidant activity in vitro, and has shown to scavenge free radicals and also to improve the reduced glutathione levels in pancreatic homogenates. The present investigation evaluates the protective effect of 1 against oxidative injury in normal and streptozotocin (STZ)-induced diabetic rats. The i.p. administration of 1 at a dose of 100 mg/kg body weight for 5 d, significantly decreased blood glucose levels and improved the endogenous antioxidant system. Also, a significant increase in the activities of the antioxidant enzymes glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) occurred. Combined treatment of rats with 1 (100 mg/kg) and STZ significantly reduced the pancreatic levels of thiobarbituric acid reactive substances (TBARS) levels. Likewise, significant increases in the activities of the antioxidant enzymes together with a decrease in blood glucose levels in both treatments were observed. The results demonstrate and support the relationship between the hypoglycemic and antioxidant activities displayed by the natural compound 1.

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