scholarly journals Detection of Bronchial Neoplasia in Uranium Miners by Autofluorescence Endoscopy (SAFE-1000)

1999 ◽  
Vol 5 (2) ◽  
pp. 91-98 ◽  
Author(s):  
T. Horvath ◽  
M. Horvathova ◽  
F. Salajka ◽  
B. Habanec ◽  
L. Foretova ◽  
...  
Keyword(s):  
White Light ◽  
Risk Group ◽  
Working Hours ◽  
Bronchial Epithelium ◽  
Bronchial Biopsy ◽  
Uranium Miners ◽  
Autofluorescence Bronchoscopy ◽  
Hematoxylin And Eosin ◽  
Autofluorescence Endoscopy ◽  
Histopathology Examination

The increase in the detection rate for premalignant changes of bronchial epithelium was studied in 56 symptom-free volunteers from the risk group of Czech uranium miners (mean age 50.69 years, mean WLM 21.06 (1 Working Level Month is equal to the absorption of latent energy of 2.08 × 10–5 J/m3 in one month, i.e. 170 working hours)) by the additional employment of the System of Autofluorescence Endoscopy (SAFE-1000 Pentax) to conventional white-light bronchoscopy, comparing results with those of bronchial biopsy histopathology examination. Histopathology using hematoxylin and eosin staining confirmed intraepithelial neoplasias in 15 areas in 10 persons. White-light bronchoscopy sensitivity was 21.05%, and specificity 93.7% which an autofluorescence bronchoscopy sensitivity was 78.95% and specificity 81.89%.

A comparison of white light laryngostroboscopy versus autofluorescence endoscopy in the evaluation of vocal fold pathology

2013 ◽  
Vol 123 (7) ◽  
pp. 1729-1734 ◽  
Author(s):  
Philipp P. Caffier ◽  
Bernd Schmidt ◽  
Manfred Gross ◽  
Klaus Karnetzky ◽  
Tadeus Nawka ◽  
...  
Keyword(s):  
White Light ◽  
Vocal Fold ◽  
Autofluorescence Endoscopy

scholarly journals Expression of urocortin in rat lung and its effect on pulmonary vascular permeability

2006 ◽  
Vol 189 (1) ◽  
pp. 167-178 ◽  
Author(s):  
Yuqing Wu ◽  
Yinyan Xu ◽  
Hong Zhou ◽  
Jin Tao ◽  
Shengnan Li
Keyword(s):  
Receptor Antagonist ◽  
Vascular Permeability ◽  
Alveolar Epithelium ◽  
Bronchial Epithelium ◽  
Rat Lung ◽  
Pulmonary Vascular Permeability ◽  
Peripheral Tissues ◽  
The Central Nervous System ◽  
Hematoxylin And Eosin ◽  
Lung Vascular Permeability

Urocortin (UCN), a newly identified, 40-amino-acid, corticotropin-releasing hormone (CRH) structurally related peptide, has been demonstrated to be expressed in the central nervous system and many peripheral tissues of rats and man. This study aimed to investigate the expression profile of UCN in rat lung and the effect of UCN on lung vascular permeability. The expression of UCN mRNA was detected by reverse transcriptase PCR (RT–PCR). UCN peptide was measured by immunohistochemistry and Western blot analysis. We found that both UCN mRNA and peptide were obviously expressed in rat lung. Immunohistochemistry results showed that UCN peptide is mainly expressed in bronchial epithelium mucosa and alveolar epithelium. We also found that rats receiving inhalation aerosol of UCN had a significant elevation of lung vascular permeability compared with rats receiving vehicle and ovalbumin (OVA) by the Evans blue (EB) technique. UCN aerosol inhalation resulted in obvious pulmonary congestion and edema observed under light microscope by hematoxylin and eosin (HE) staining. The nonselective peptide CRH receptor antagonist astressin markedly reduced lung vascular permeability triggered by UCN. Enhanced pulmonary vascular permeability induced by UCN was markedly inhibited by pretreatment with the mast-cell stabilizer cromolyn and histamine-1 (H1) receptor antagonist azelastine respectively, but not by the leukotriene receptor antagonist montelukast. In summary, in the present study, we demonstrated for the first time that UCN is expressed in rat lung and contributes to an increase in lung vascular permeability through activation of CRH receptors. Mast cells and histamine may be involved in this effect of UCN. Peripherally produced UCN in lung may act as an autocrine and paracrine proinflammatory factor.

scholarly journals STUDIES ON INFLUENZA INFECTION IN FERRETS BY MEANS OF FLUORESCEIN-LABELLED ANTIBODY

1955 ◽  
Vol 101 (6) ◽  
pp. 665-676 ◽  
Author(s):  
Ch'ien Liu
Keyword(s):  
Epithelial Cells ◽  
Influenza Infection ◽  
Bronchial Epithelium ◽  
Mediastinal Lymph Nodes ◽  
Viral Antigens ◽  
Antibody Staining ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Ciliated Epithelial Cells ◽  
Infectivity Titer

By means of fluorescein-labelled antibody staining, specific influenza viral antigens were seen in both the cytoplasm and the nucleus of infected ciliated epithelial cells covering the nasal turbinates of infected ferrets. Initially, only a small portion of the nasal epithelium showed fluorescence, and no appreciable abnormality of the cells could be detected by hematoxylin and eosin staining. The fluorescence soon spread to involve the entire epithelium, followed by desquamation coinciding with the onset of manifest illness. Pneumonia was seen in some of the infected ferrets, and in them, viral antigens were found in the bronchial epithelium and in the mediastinal lymph nodes. A rise of viral infectivity titer paralleled the observed spread of viral antigens. Many desquamated nasal epithelial cells and macrophages containing antigen were present in nasal smears. The finding would seem to offer a method for the rapid specific diagnosis of influenza infection.

Randomized placebo-controlled trial of isotretinoin in chemoprevention of bronchial squamous metaplasia.

1994 ◽  
Vol 12 (5) ◽  
pp. 937-945 ◽  
Author(s):  
J S Lee ◽  
S M Lippman ◽  
S E Benner ◽  
J J Lee ◽  
J Y Ro ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Controlled Trial ◽  
Squamous Metaplasia ◽  
Bronchial Epithelium ◽  
Controlled Study ◽  
Bronchial Biopsy ◽  
Treatment Groups ◽  
Major Activity ◽  
Complete Reversal ◽  
Preclinical And Clinical Studies

PURPOSE Retinoids have proven chemopreventive efficacy in both preclinical and clinical studies. This trial was designed to confirm the finding of an earlier uncontrolled trial that the synthetic retinoid etretinate had major activity in reversing squamous metaplasia found in the bronchial epithelium of chronic smokers. PATIENTS AND METHODS We prospectively evaluated 152 smokers with bronchoscopy and obtained biopsies from six sites. Subjects with dysplasia and/or a metaplasia index of greater than 15% were randomly assigned to receive either 1 mg/kg isotretinoin or placebo daily for 6 months. Of 86 subjects randomized (41 isotretinoin, 45 placebo), 69 were reevaluated at the completion of treatment. RESULTS In the group as a whole, the metaplasia index decreased over time from a mean +/- SE of 35.8% +/- 2.7% at baseline to 28.1% +/- 3.3% at the completion of treatment (P = .01) by repeated measures analysis of variance [ANOVA]); a reduction in the metaplasia index (> 8%) was noted in both isotretinoin and placebo groups (19 of 35 [54.3%] and 20 of 34 [58.8%], respectively). Complete reversal of squamous metaplasia was noted in nine subjects from each group. However, the magnitudes of the mean metaplasia index changes did not differ significantly in the two treatment groups. In both groups, smoking cessation resulted in significant declines in the extent of squamous metaplasia, whereas no significant change in metaplasia index was found among those who continued to smoke. CONCLUSION Squamous metaplasia was frequently observed in bronchial biopsy samples from chronic smokers. From this study, we conclude that isotretinoin has no effect on squamous metaplasia, a potential intermediate end point of bronchial carcinogenesis. Although determining the exact role of isotretinoin in lung cancer prevention requires further study, the finding that there was a significant decrease in squamous metaplasia in the placebo group emphasizes the critical importance of a placebo-controlled study design in chemoprevention trials using intermediate end points.

Autofluorescence Endoscopy Allows Better Differentiation Than White Light Video Colonoscopy in Classifying Adenomatous and Non-Adenomatous Colorectal Polyps

2009 ◽  
Vol 69 (5) ◽  
pp. AB290 ◽  
Author(s):  
Paul G. Van Putten ◽  
D. Ramsoekh ◽  
Jelle Haringsma ◽  
Jan-Werner Poley ◽  
Herman Van Dekken ◽  
...  
Keyword(s):  
White Light ◽  
Colorectal Polyps ◽  
Autofluorescence Endoscopy ◽  
Video Colonoscopy

O-164 A multi-center study comparing autofluorescence bronchoscopy to white light bronchoscopy

Lung Cancer ◽  
2003 ◽  
Vol 41 ◽  
pp. S49-S50 ◽  
Author(s):  
John F. Beamis ◽  
Armin Ernst ◽  
Praveen Mathur ◽  
Rex Yung ◽  
Michael Simoff
Keyword(s):  
White Light ◽  
Autofluorescence Bronchoscopy ◽  
Multi Center Study ◽  
Center Study

A Multicenter Study Comparing Autofluorescence Bronchoscopy to White Light Bronchoscopy Using a Non-Laser Light Stimulation System

CHEST Journal ◽  
2004 ◽  
Vol 125 (5) ◽  
pp. 148S-149S ◽  
Author(s):  
John F. Beamis ◽  
Armin Ernst ◽  
Michael Simoff ◽  
Rex Yung ◽  
Praveen Mathur
Keyword(s):  
Multicenter Study ◽  
White Light ◽  
Laser Light ◽  
Light Stimulation ◽  
Autofluorescence Bronchoscopy ◽  
Stimulation System
Sign in / Sign up

Export Citation Format

Share Document