scholarly journals Purification and Preliminary Characterization of Tetraheme Cytochrome c3 and Adenylylsulfate Reductase from the Peptidolytic Sulfate-Reducing Bacterium Desulfovibrio aminophilus DSM 12254

2005 ◽  
Vol 3 (1-2) ◽  
pp. 81-91 ◽  
Author(s):  
Alejandro López-Cortés ◽  
Sergey Bursakov ◽  
Angelo Figueiredo ◽  
Anders E. Thapper ◽  
Smilja Todorovic ◽  
...  
Keyword(s):  
Cytochrome C3 ◽  
Preliminary Characterization ◽  
Sulfate Reducing ◽  
Tetraheme Cytochrome

A hypothetical model of the flavodoxin-tetraheme cytochrome c3 complex of sulfate-reducing bacteria

Biochemistry ◽  
1988 ◽  
Vol 27 (7) ◽  
pp. 2444-2450 ◽  
Author(s):  
D. E. Stewart ◽  
J. LeGall ◽  
I. Moura ◽  
J. J. G. Moura ◽  
H. D. Peck ◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Cytochrome C3 ◽  
Hypothetical Model ◽  
Sulfate Reducing ◽  
Tetraheme Cytochrome ◽  
Reducing Bacteria

scholarly journals Cytochrome c3 Mutants ofDesulfovibrio desulfuricans

2000 ◽  
Vol 66 (2) ◽  
pp. 671-677 ◽  
Author(s):  
Barbara J. Rapp-Giles ◽  
Laurence Casalot ◽  
R. Samuel English ◽  
Joseph A. Ringbauer ◽  
Alain Dolla ◽  
...  
Keyword(s):  
Physiological Role ◽  
Desulfovibrio Desulfuricans ◽  
Physical Analysis ◽  
Cytochrome C3 ◽  
Transport Pathway ◽  
Gene Encoding ◽  
Sulfate Reducing ◽  
Tetraheme Cytochrome ◽  
Cell Densities

ABSTRACT To explore the physiological role of tetraheme cytochromec 3 in the sulfate-reducing bacteriumDesulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated plasmid showed that the insertion was successful. The growth rate of the mutant on lactate with sulfate was comparable to that of the wild type; however, mutant cultures did not achieve the same cell densities. Pyruvate, the oxidation product of lactate, served as a poor electron source for the mutant. Unexpectedly, the mutant was able to grow on hydrogen-sulfate medium. These data support a role for tetraheme cytochromec 3 in the electron transport pathway from pyruvate to sulfate or sulfite in D. desulfuricans G20.

scholarly journals Mossbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743). Spectral deconvolution of the heme components

1992 ◽  
Vol 204 (2) ◽  
pp. 779-782 ◽  
Author(s):  
Natarajan RAVI ◽  
Isabel MOURA ◽  
Cristina COSTA ◽  
Miguel TEIXEIRA ◽  
Jean LeGALL ◽  
...  
Keyword(s):  
Cytochrome C3 ◽  
Spectral Deconvolution ◽  
Tetraheme Cytochrome

Isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism Desulfovibrio desulfuricans ATCC 27774

Anaerobe ◽  
1995 ◽  
Vol 1 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Sergey Bursakov ◽  
Ming-Y. Liu ◽  
William J. Payne ◽  
Jean LeGall ◽  
Isabel Moura ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Desulfovibrio Desulfuricans ◽  
Preliminary Characterization ◽  
Sulfate Reducing

Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

1982 ◽  
Vol 47 (02) ◽  
pp. 128-131 ◽  
Author(s):  
F Esnard ◽  
E Dupuy ◽  
A M Dosne ◽  
E Bodevin
Keyword(s):  
Endothelial Cells ◽  
Primary Culture ◽  
Ammonium Sulphate ◽  
Concanavalin A ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Preliminary Characterization ◽  
Umbilical Vein Endothelial Cells ◽  
Ammonium Sulphate Saturation

SummaryA preliminary characterization of a fibrinolytic inhibitor released by human umbilical vein endothelial cells in primary culture is reported. This molecule of Mr comprised between 2 × 105 and 106 and of μ2 mobility precipitates at 43% ammonium sulphate saturation and is totally adsorbed on Concanavalin A Sepharose 4 B. A possible relationship with a macroglobulins is discussed.

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