scholarly journals Elimination of Aspergillus flavus from Pistachio Nuts with Dielectric Barrier Discharge (DBD) Cold Plasma and Its Impacts on Biochemical Indices

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mina Makari ◽  
Mohammad Hojjati ◽  
Samira Shahbazi ◽  
Hamed Askari
Keyword(s):  
Antioxidant Activity ◽  
Plasma Treatment ◽  
Aspergillus Flavus ◽  
Cold Plasma ◽  
Phenolic Content ◽  
Barrier Discharge ◽  
Total Phenolic ◽  
Dielectric Barrier ◽  
Pistachio Nuts ◽  
Spore Population

In the present research, the effects of different durations (0, 15, 30, 60, 90, 120, 150, and 180 sec) of dielectric barrier discharge (DBD) cold plasma on decontaminating Aspergillus flavus, detoxifying pure aflatoxin B1 (AFB1), and the quality attributes of pistachio nuts (total phenolic content, antioxidant activity, chlorophylls, total carotenoids, instrumental color, total soluble protein, and malondialdehyde determination) were studied. The results showed that the viable spore population reduced with the increase of plasma treatment duration, so that after 180 s of the treatment, a decrease by 4 logs was observed in the spore population. Chlorophyll a and b, as well as total carotenoid levels and color parameters, decreased, which led to darker pistachio samples and intensity reduction in soluble protein content and protein bands. Plasma treatment did not alter the total phenolic content but slightly increased the antioxidant activity of pistachio nuts samples. The malondialdehyde values significantly increased all the plasma treatment durations. The maximum reduction of AFB1 was observed after 180 s of the treatment, which was 64.63% and 52.42% for glass slides and pistachio nut samples, respectively. The present findings demonstrated that cold plasma could be used as an efficient decontamination method of food products without inducing undesirable quality changes in nuts.

scholarly journals Cold Plasma Treatment on Mustard Green Seeds and its Effect on Growth, Isothiocyanates, Antioxidant Activity and Anticancer Activity of Microgreens

2021 ◽  
Vol 25 (03) ◽  
pp. 667-676
Author(s):  
Worachot Saengha
Keyword(s):  
Antioxidant Activity ◽  
Plasma Treatment ◽  
Anticancer Activity ◽  
Cold Plasma ◽  
Allyl Isothiocyanate ◽  
Total Phenolic ◽  
Anticancer Properties ◽  
Bioactive Content ◽  
Mustard Green ◽  
Mcf 7

The aims of this work were to study growth, isothiocyanate (ITC), bioactive content, antioxidant activity and anticancer activity of mustard green (MG) microgreens grown from seeds treated with cold plasma at 21 and 23 kV for 5 min. Microgreens from plasma-treated seeds at 23 kV showed almost 2-fold increased ITC content (1.57 ± 0.05 mmol/100 g DW) compared to MG from seeds without plasma (control), showed the highest total phenolic content (TPC) (6.76 ± 0.14 mg GAE/g DW) and total flavonoid content (TFC) (0.16±0.01 mg RE/g DW). However, MG plasma-treated seeds at 21 kV showed the highest antioxidant activity from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay (3.51 ± 0.38 mg TE/g DW). Allyl isothiocyanate and 3-butenyl isothiocyanate were the dominant ITCs in MG. The highest cytotoxicities using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against MCF-7 (IC50 of 32.44 ± 1.64 μg/mL) and HepG2 (IC50 of 28.58 ± 1.04 μg/mL) after 72 h exposure were found in MG from plasma-treated seeds at 23 kV and MG from control seeds, respectively. However, MG from plasma-treated seeds at 21 kV exhibited the highest antiproliferative effect against MCF-7 (IC50 of 23.23 ± 0.23 μg/mL) and HepG2 (IC50 of 20.44 ± 0.56 μg/mL) for 14 days and also the most potent antimigratory effect. MG from cold plasma inhibited MMP-9 protein expression in both cancers indicating antimigratory property. MG from cold plasma also significantly reduced MMP-9 mRNA expression in both cancers when compared to the control and untreated cells. In conclusion, cold plasma treatment on seeds seemed to be an innovative tool to enhance ITC, TPC, TFC and anticancer properties of MG microgreens for better health implications. © 2021 Friends Science Publishers

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