scholarly journals Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Bin Han ◽  
Yanhui Zhang ◽  
Yuxia Xiao ◽  
Bohong Shi ◽  
Hong Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Extracellular Vesicles ◽  
Oral Submucous Fibrosis ◽  
Precancerous Lesion ◽  
Oral Diseases ◽  
Collagen Iii ◽  
Submucous Fibrosis ◽  
Related Proteins ◽  
Adipose Derived Stem Cell

Oral submucous fibrosis (OSF) is a precancerous lesion. Adipose-derived stem cell- (ADSC-) derived extracellular vesicles (EVs) (ADSC-EVs) regulate multiple oral diseases. Hence, this study explored the mechanism of ADSC-EVs in OSF. ADSCs were transduced with microRNA- (miR-) 375 mimic. ADSC-EVs and miR-375-overexpressed ADSC-EVs (EVs-miR-375) were extracted and identified. miR-375 expression in EVs and fibrotic buccal mucosal fibroblasts (fBMFs) was detected. EV uptake by fBMFs was observed. The targeted relationship between miR-375 and forkhead box protein F1 (FOXF1) was predicted and verified. After EVs-miR-375 treatment or FOXF1 overexpression, fBMF cell proliferation, migration, invasion, and apoptosis were evaluated, and levels of apoptosis-related proteins (cleaved-caspase-3, Bax, and Bcl-2) and fibrosis markers (α-SMA, collagen I, and collagen III) were detected. Functional rescue experiments were further performed to verify the role of the miR-375/FOXF1 axis in OSF. miR-375 was notably upregulated in EVs-miR-375 and EVs-miR-375-treated fBMFs (all P < 0.001 ). ADSC-EVs carried miR-375 into fBMFs. fBMFs can internalize ADSC-EVs. EVs-miR-375 treatment markedly inhibited fBMF cell proliferation, migration, invasion, and fibrosis and promoted apoptosis (all P < 0.01 ). Moreover, miR-375 targeted FOXF1 in fBMFs. FOXF1 overexpression promoted fBMF cell biological behaviors and fibrosis, which were reversed after EVs-miR-375 treatment ( P < 0.01 or P < 0.001 ). We highlighted that ADSC-EVs inhibited fBMF fibrosis and then suppressed OSF progression via the miR-375/FOXF1 axis.

Evaluation of aberrant metabolism related proteins in oral submucous fibrosis: A pilot study

2018 ◽  
Vol 60 (4) ◽  
pp. 87-91 ◽  
Author(s):  
Vertika Rai ◽  
Surajit Bose ◽  
Rashmi Mukherjee ◽  
Arpita Sarbajna ◽  
Chandan Chakraborty
Keyword(s):  
Pilot Study ◽  
Oral Submucous Fibrosis ◽  
Submucous Fibrosis ◽  
Related Proteins

scholarly journals Mesenchymal stem cell-derived extracellular vesicles may promote breast cancer cell dormancy

2018 ◽  
Vol 9 ◽  
pp. 204173141881009 ◽  
Author(s):  
Jake Casson ◽  
Owen G Davies ◽  
Carol-Anne Smith ◽  
Matthew J Dalby ◽  
Catherine C Berry
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Extracellular Vesicles ◽  
Breast Cancer Cells ◽  
Cell Dormancy

Disseminated breast cancer cells have the capacity to metastasise to the bone marrow and reside in a dormant state within the mesenchymal stem cell niche. Research has focussed on paracrine signalling factors, such as soluble proteins, within the microenvironment. However, it is now clear extracellular vesicles secreted by resident mesenchymal stem cells into this microenvironment also play a key role in the initiation of dormancy. Dormancy encourages reduced cell proliferation and migration, while upregulating cell adhesion, thus retaining the cancer cells within the bone marrow microenvironment. Here, MCF7 breast cancer cells were treated with mesenchymal stem cell–derived extracellular vesicles, resulting in reduced migration in two-dimensional and three-dimensional culture, with reduced cell proliferation and enhanced adhesion, collectively supporting cancer cell dormancy.

scholarly journals A clinical study on precancerous lesions of oral cavity in patients attending dental hospital, Telangana

2019 ◽  
Vol 6 (11) ◽  
pp. 4739
Author(s):  
Soundarya Hanumakonda ◽  
Marri Swetha ◽  
Mallela Bhashitha ◽  
Harsha Mudigonda
Keyword(s):  
Oral Cavity ◽  
Oral Lichen Planus ◽  
Precancerous Lesions ◽  
Oral Submucous Fibrosis ◽  
Age Distribution ◽  
Precancerous Lesion ◽  
Number Of Patients ◽  
Dental Hospital ◽  
Submucous Fibrosis ◽  
Oral Lichen

Background: Aim of the retrospective study is to determine the type and frequency of the precancerous lesions of the oral cavity and to assess their sex, age distribution among the patients.Methods: Study was conducted based on year-wise data collected during 2015 to 2018 of all the patients with precancerous lesions of oral cavity.Results: The total number of patients was 1247, out of which 803 (64.39%) were males and 444 (35.60%) were females. Most common precancerous lesion is oral submucous fibrosis (70.24%) followed by oral lichen planus (19.08%), leukoplakia (8.98%), erythroplakia (1.68%).Conclusions: We observed marked geographical variation in the incidences of various precancerous lesions. Mostly males are affected by precancerous lesions than females.

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