scholarly journals Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Yudianto Budi Saroyo ◽  
Noroyono Wibowo ◽  
Rima Irwinda ◽  
Ani Retno Prijanti ◽  
Evy Yunihastuti ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Telomere Length ◽  
Clinical Signs ◽  
High Mobility ◽  
Critical Length ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Maternal Characteristics ◽  
Term Birth

Introduction. Senescent cells have been demonstrated to release High Mobility Group Box 1 (HMGB1) which induces labor through an inflammatory pathway. This research is aimed at demonstrating whether telomere shortening, proinflammatory HMGB1, and oxidative damage marker 8-OHdG play a role in the placenta of preterm birth in comparison to term birth. Method. A cross-sectional study on 67 full thickness of the placenta obtained from mothers with term and preterm birth. Mothers with clinical signs of infection ( fever > 38 ° C , leukocytosis > 18000 / μ L , or abnormal vaginal discharge) and other pregnancy complications were excluded. Real-time polymerase chain reaction was performed to measure T/S ratio and ELISA quantification to measure the amount of HMGB1 and 8-OHdG. Result. A total of 34 placentas from preterm and 33 placentas from term birth were examined. Maternal characteristics were comparable between the two groups. There were no statistical difference of T/S ratio ( p = 0.181 ), HMGB1 ( p = 0.119 ), and 8-OHdG ( p = 0.144 ) between the preterm and term groups. HMGB1 was moderately correlated with 8-OHdG ( r = 0.314 ). Telomere T/S ratio of the placenta did not differ between preterm and term labor despite difference in gestational age, suggesting earlier shortening in the preterm group. It is possible that critical telomere length has been achieved in both term and preterm placenta that warrants labor through senescence process. The result of our study also showed that HMGB1 was not correlated to telomere length, due to the fact that HMGB1 is not upregulated until the critical length of telomere for senescence is exhibited. Conclusion. Similar telomere length might be exhibited due to early telomere shortening in preterm birth that mimics the term placenta. The relationship between placental telomere shortening and HMGB1 release remains to be uncovered. Further research is needed to discover the factors leading to early telomere shortening in the placenta of preterm birth.

scholarly journals A multidisciplinary approach to mental illness: do inflammation, telomere length and microbiota form a loop? A protocol for a cross-sectional study on the complex relationship between inflammation, telomere length, gut microbiota and psychiatric disorders

BMJ Open ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. e032513 ◽  
Author(s):  
Mirko Manchia ◽  
Pasquale Paribello ◽  
Carlo Arzedi ◽  
Alberto Bocchetta ◽  
Paola Caria ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Psychiatric Disorders ◽  
Telomere Length ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Sectional Study ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Age Related ◽  
The University

IntroductionSevere psychiatric disorders are typically associated with a significant reduction in life expectancy compared with the general population. Among the different hypotheses formulated to explain this observation, accelerated ageing has been increasingly recognised as the main culprit. At the same time, telomere shortening is becoming widely accepted as a proxy molecular marker of ageing. The present study aims to fill a gap in the literature by better defining the complex interaction/s between inflammation, age-related comorbidities, telomere shortening and gut microbiota in psychiatric disorders.Methods and analysisA cross-sectional study is proposed, recruiting 40 patients for each of three different diagnostic categories (bipolar disorder, schizophrenia and major depressive disorder) treated at the Section of Psychiatry and at the Unit of Clinical Pharmacology of the University Hospital Agency of Cagliari (Italy), compared with 40 age-matched and sex-matched non-psychiatric controls. Each group includes individuals suffering, or not, from age-related comorbidities, to account for the impact of these medical conditions on the biological make-up of recruited patients. The inflammatory state, microbiota composition and telomere length (TL) are assessed.Ethics and disseminationThe study protocol was approved by the Ethics Committee of the University Hospital Agency of Cagliari (PG/2018/11693, 5 September 2018). The study is conducted in accordance with the principles of good clinical practice and the Declaration of Helsinki, and in compliance with the relevant Italian national legislation. Written, informed consent is obtained from all participants. Participation in the study is on a voluntary basis only. Patients will be part of the dissemination phase of the study results, during which a local conference will be organised and families of patients will also be involved. Moreover, findings will be published in one or more research papers and presented at national and international conferences, in posters or oral communications.

scholarly journals The Correlation between Psychosocial Stress and Prematurity

2016 ◽  
pp. 55-60
Author(s):  
Renny Aditya
Keyword(s):  
Preterm Birth ◽  
Psychosocial Stress ◽  
Major Change ◽  
Cross Sectional Study ◽  
Living Conditions ◽  
Chi Square ◽  
Cross Sectional ◽  
Term Birth ◽  
Chi Square Test ◽  
The Relationship

Objective: This study was aimed to analyze the relationship between the level of psychosocial stress on preterm birth and term birth. Methods: This was a cross-sectional study, with 45 patients experiencing preterm birth and at term birth, who met the inclusion and exclusion criterias in Obstetrics and Gynecology Department of Dr. Hasan Sadikin Hospital and other hospital nearby. Result: The level of psychosocial stress on preterm birth was severe 64.4 %, moderate 14% and mild 2%. The comparison between the group resulted in p < 0.001 using chi-square test with confidence interval 95% and correlation coefficient was phi = 0.811. The specific psychosocial stressor were problem with in laws (p=0.001), problem with husband (p=0.003), foreclosure on a mortgage or loan (p=0.001), change in residence (p=0.001), major change in living conditions (building a new home, remodeling) (p=0.001), no monetary possession (p=0.001), major change in financial state (p=0.004), environment vulnerability (p=0.035), and being dismissal from work (p=0.014). Conclusion: The relationship between psychosocial stress and preterm birth was significant. The stressor included problem with in laws foreclosure on a mortgage or loan, change in residence, major change in living conditions (building a new home, remodeling, no monetary possesion, major change in financial state, environment vulnerability, and dismissal from work. [Indones J Obstet Gynecol 2012; 36-2: 55-60] Keywords: preterm birth, psychosocial stres

scholarly journals Long-Term Outcomes of Accelerated Telomere Shortening in Acquired Aplastic Anemia

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 4396-4396
Author(s):  
Alexander Kulagin ◽  
Vyacheslav Borisov ◽  
Natalia Pronkina ◽  
Irina Kruchkova ◽  
Igor Lisukov ◽  
...  
Keyword(s):  
Aplastic Anemia ◽  
Telomere Length ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Long Term Outcomes ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Increased Risk ◽  
Treatment Naïve

Abstract Introduction: Accelerated telomere shortening (TS) in blood cells has been well documented in both dyskeratosis congenita and acquired aplastic anemia (AA). The present study investigated the incidence and long-term outcomes of TS in AA patients treated with ATG plus cyclosporine immunosuppressive therapy (IST). Methods: A total of 53 patients (31 M and 22 F, median age 24, 9-62) with moderate (MAA 15), severe (SAA 22) and very severe (vSAA 16) AA were included in cross-sectional study of telomere length (TEL) between 2008 and 2009 and then followed prospectively for 5 years. Treatment-naïve, recovering after IST and refractory AA were in 13, 35 and 5 patients respectively. There were no cases with physical abnormalities or family history of hematological disorders. TEL in granulocytes was assessed by Flow-FISH method with calculation of absolute and age-adjusted (DeltaTEL) values. TS was defined as absolute TEL value below the normal 99% confidence interval in age matched healthy donors (n=71). Outcome measures were response rate in treatment-naïve patients, cumulative incidence (CI) of relapse and clonal evolution, overall (OS) and failure-free survival (FFS). Adult patients or parents of children signed informed consent. Results: TS was detected in 24 patients (45%) with a median DeltaTEL of -1.715 kbp compared to -0.009 kbp in 29 patients with normal telomere length (TN) (Table 1). Table 1. Patient and disease characteristics in cross-sectional study TS (n=24) TN (n=29) P DeltaTEL, kbp, median (range) -1.715 (-5.100 – -0.983) -0.009 (-0.760 – 4.142) <0.001 Age, years, median (range) 22.5 (9-37) 26 (14-62) 0.102 Gender, M/F, n 15/9 16/13 0.780 Severity, n (%) MAA 10 (42%) 5 (17%) 0.0485 SAA/vSAA 14 (58%) 24 (83%) Response to prior IST, n (%) Treatment-naïve 4 (17%) 9 (31%) 0.338 Partial response 7 (29%) 9 (31%) Complete response 8 (33%) 11 (38%) Refractory 5 (21%) 0 (0%) 0.047 PNH positive, n/n (%) 12/24 (50%) 13/28 (46%) 1.000 Interval between AA onset and IST, days, median (range) 92 (17-508) 102 (26-585) 0.848 Interval between AA onset and TEL testing, days, median (range) 1093 (49-3792) 785 (64-3494) 0.173 TS was associated with MAA (p = 0.049) and refractory disease (p=0.047) in cross-sectional study. No significant differences in gender, median age, baseline blood counts, PNH positivity or interval from AA onset and IST were observed between the two groups. Median follow-up after IST start and TEL testing were 90 and 67 months respectively. Response rates to IST were similar in TS (3/4, 75%) and TN (6/9, 67%) patients evaluated prospectively (p=1.0). TS and TN patients showed similar 10-year CI of relapse (16% vs 14%, p=0.905) and hemolytic PNH (28% vs 20%, p=0.532). However, all 3 cases of MDS /AML occurred in patients with TS with CI of 15.4% vs. 0% in the TN (p = 0.063). Compared patient groups have similar high probability of 10-year OS (TS 86.9% vs TN 92.6%, p=0.571) due to the large proportion of patients responding to treatment. The FFS rates were much lower (38.5% and 57.5% respectively, p=0.510). Conclusions: TS is a relatively common phenomenon in patients with otherwise typical acquired AA and more frequently detected in MAA and refractory to IST cases in cross-sectional study. Our data confirm the previously reported association of TS and increased risk of MDS/AML, which should be considered for a more careful monitoring and treatment planning, including allogeneic BMT as a curative approach. Disclosures No relevant conflicts of interest to declare.

Telomere Length, SIRT1, and Insulin in Male Master Athletes: The Path to Healthy Longevity?

2021 ◽  
Author(s):  
Samuel S. Aguiar ◽  
Thiago S. Rosa ◽  
Rodrigo V. P. Neves ◽  
Patrício L. A. Leite ◽  
Larissa A. Maciel ◽  
...  
Keyword(s):  
Telomere Length ◽  
Cross Sectional Study ◽  
Biological Aging ◽  
Master Athletes ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Healthy Longevity ◽  
Relative Telomere Length ◽  
Age Related ◽  
Commercial Kits

AbstractLower SIRT1 and insulin resistance are associated with accelerated telomere shortening. This study investigated whether the lifestyle of master athletes can attenuate these age-related changes and thereby slow aging. We compared insulin, SIRT1, and telomere length in highly trained male master athletes (n=52; aged 49.9±7.2 yrs) and age-matched non-athletes (n=19; aged 47.3±8.9 yrs). This is a cross-sectional study, in which all data were collected in one visit. Overnight fasted SIRT1 and insulin levels in whole blood were assessed using commercial kits. Relative telomere length was determined in leukocytes through qPCR analyses. Master athletes had higher SIRT1, lower insulin, and longer telomere length than age-matched non-athletes (p<0.05 for all). Insulin was inversely associated with SIRT1 (r=−0.38; p=0.001). Telomere length correlated positively with SIRT1 (r=0.65; p=0.001), whereas telomere length and insulin were not correlated (r=0.03; p=0.87). In conclusion, master athletes have higher SIRT1, lower insulin, and longer telomeres than age-matched non-athletes. Furthermore, SIRT1 was negatively associated with insulin and positively associated with telomere length. These findings suggest that in this sample of middle-aged participants reduced insulin, increased SIRT1 activity, and attenuation of biological aging are connected.

SIGNIFICANCE OF PLACENTAL HISTOPATHOLOGICAL FINDINGS IN PRETERM/TERM BIRTH

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Monika Kushwaha ◽  
Sanjeev Narang
Keyword(s):  
Preterm Birth ◽  
Comparative Study ◽  
Pregnancy Outcome ◽  
Neonatal Morbidity ◽  
Research Centre ◽  
College Hospital ◽  
Cross Sectional ◽  
Chorionic Villi ◽  
Term Birth ◽  
Medical College

Background: This study is cross-sectional, observational and comparative study, at Index Medical College, Hospital & Research Centre, Indore, Madhya Pradesh from July 2017 to July 2019 with sample size 100 placentae. Method: The placenta received was evaluated blinded of maternal pregnancy outcome. The pattern of morphology was evaluated both qualitatively (type of lesion) and quantitatively (number of lesions). Result: In Present study 79% of the deliveries were term deliveries and 21% were preterm deliveries. On placental macroscopy, placenta weight was significantly low among the neonates of preterm deliveries (370.00±60.49) as compared to term deliveries (440.89±55.22). Preterm placenta had higher number of abnormal placental lesion compared to term pregnancies. Conclusion: The uteroplacental insufficiency defined as placental infarct, fibrosis of chorionic villi, thickening of blood vessels, and poor vascularity of chorionic villi. Placental histopathological lesions are strongly associated with maternal under perfusion and uteroplacental insufficiency. These are the reasons for preterm birth. Thus, knowledge of the etiological factor can be use to reduce maternal and neonatal morbidity and mortility. Keywords: Placenta, Term & Preterm.

scholarly journals Long-chain polyunsaturated fatty acid (LC-PUFA) status in severe preeclampsia and preterm birth: a cross sectional study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rima Irwinda ◽  
Rabbania Hiksas ◽  
Aprilia Asthasari Siregar ◽  
Yudianto Budi Saroyo ◽  
Noroyono Wibowo
Keyword(s):  
Fatty Acid ◽  
Preterm Birth ◽  
Cross Sectional Study ◽  
Severe Preeclampsia ◽  
Chain Polyunsaturated Fatty Acid ◽  
Omega 3 ◽  
Sectional Study ◽  
Cross Sectional ◽  
Omega 6 ◽  
Long Chain

AbstractLong-Chain Polyunsaturated Fatty Acid (LCPUFA) is essential throughout pregnancy, since deficiency of LPUFA may linked to obstetrical complications. This study aimed to investigate LCPUFA status in severe preeclampsia and preterm birth. A cross sectional study was conducted in 104 pregnant women, which divided into normal pregnancy, severe preeclampsia and preterm birth groups. Serum percentage and concentration of total LCPUFA, omega-3, alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), omega-6, linoleic acid (LA), and arachidonic acid (AA) were measured using gas chromatography/mass spectrometry. Receiver operating characteristic (ROC), bivariate and multivariate analysis were performed. Severe preeclampsia showed the highest concentration of total PUFA and the lowest DHA percentage, with significantly higher Omega-6/Omega-3 ratio (p = 0.004) and lower omega-3 index (p < 0.002) compared to control. Preterm birth showed the least omega-3 concentrations, with significantly low omega-6 derivates (LA (p = 0.014) and AA (p = 0.025)) compared to control. LCPUFA parameters have shown to increase the risk in both conditions, particularly ALA ≤ 53 µmol/L in preeclampsia with OR 5.44, 95%CI 1.16–25.42 and preterm birth with OR 4.68, 95%CI 1.52–14.38. These findings suggest that severe preeclampsia and preterm birth have an imbalance in LCPUFA status.

scholarly journals Magnitude of spontaneous preterm birth and its associated factors among preterm birth in NICU wards in Asella Teaching and Referral Hospital, Asella, Oromia, Ethiopia

2021 ◽  
Vol 49 (8) ◽  
pp. 030006052110346
Author(s):  
Techane Sisay Tuji ◽  
Addisu Dabi Wake ◽  
Gezahegn Badeg Adere ◽  
Aselefu Beka Wedajo ◽  
Batu Dekeba Obole ◽  
...  
Keyword(s):  
Risk Factors ◽  
Preterm Birth ◽  
Healthcare Providers ◽  
Preterm Births ◽  
Cross Sectional Study ◽  
Spontaneous Preterm Birth ◽  
Related Factors ◽  
Cross Sectional ◽  
Bivariate Logistic Regression ◽  
Associated Risk Factors

Objective To assess the prevalence of spontaneous preterm births and to identify the associated risk factors. Methods This single-centre cross-sectional study enrolled women that experienced a preterm birth as registered on the neonatal log-book between 30 December 2019 and 30 December 2020. A pre-tested structured checklist was used to collect data (sociodemographic characteristics; obstetric-related factors; medical history; and pregnancy-related factors). Bivariate logistic regression analyses were applied to identify factors associated with spontaneous preterm birth. A multivariate model identified significant independent risk factors. Results A total of 310 patients participated in the study. The prevalence of spontaneous preterm birth in this population was 67.1% (208 of 310; 95% confidence interval [CI] 61.5, 71.9). Patients without a partner (adjusted odds ratio [AOR] = 1.470, 95% CI 1.23, 4.42), patients residing in a rural area (AOR = 2.51, 95% CI 1.123, 5.513) and those with a history of PIH during their current pregnancy (AOR = 0.104, 95% CI 0.053, 0.014) were significantly more likely to have a spontaneous preterm birth. Conclusion The prevalence of spontaneous preterm birth in in this study was high. Healthcare providers and all stakeholders should focus on screening pregnant women at the risk of spontaneous preterm birth.

scholarly journals Concentrations of Carotenoids and Tocopherols in Breast Milk from Urban Chinese Mothers and Their Associations with Maternal Characteristics: A Cross-Sectional Study

Nutrients ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 1229 ◽  
Author(s):  
Yong Xue ◽  
Esther Campos-Giménez ◽  
Karine Redeuil ◽  
Antoine Lévèques ◽  
Lucas Actis-Goretta ◽  
...  
Keyword(s):  
Breast Milk ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Maternal Characteristics ◽  
Chinese Mothers

scholarly journals Leukocyte Telomere Length in Relation to 17 Biomarkers of Cardiovascular Disease Risk: A Cross-Sectional Study of US Adults

PLoS Medicine ◽  
2016 ◽  
Vol 13 (11) ◽  
pp. e1002188 ◽  
Author(s):  
David H. Rehkopf ◽  
Belinda L. Needham ◽  
Jue Lin ◽  
Elizabeth H. Blackburn ◽  
Ami R. Zota ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Telomere Length ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Cross Sectional Study ◽  
Leukocyte Telomere Length ◽  
Sectional Study ◽  
Cross Sectional

Polycyclic aromatic hydrocarbons exposures and telomere length: A cross-sectional study on preschool children

2021 ◽  
Vol 195 ◽  
pp. 110757
Author(s):  
Abolfazl Adli ◽  
Seyed Mostafa Hosseini ◽  
Moslem Lari Najafi ◽  
Maryam Behmanesh ◽  
Elahe Ghezi ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Preschool Children ◽  
Telomere Length ◽  
Aromatic Hydrocarbons ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Polycyclic Aromatic
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