scholarly journals The Effect of Stromal-Derived Factor 1α on Osteoinduction Properties of Porous β-Tricalcium Phosphate Bioceramics

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Fangchun Jin ◽  
Qixun Cai ◽  
Wei Wang ◽  
Xiaohui Fan ◽  
Xiao Lu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Tissue Repair ◽  
In Vivo Studies ◽  
New Bone Formation ◽  
Defect Model ◽  
Rabbit Bone ◽  
In Vitro Effects

β-Tricalcium phosphate (TCP) is a type of bioceramic material which is commonly used for hard tissue repair and famous of its remarkable biocompatibility and osteoconductivity with similar composition to natural bone. However, TCP lacks osteoindcutive properties. Stromal-derived factor 1α (SDF-1α) can promote bone regeneration with excellent osteoinduction effect. In this study, SDF-1α was loaded into TCP to investigate the in vitro effects of SDF-1α on the osteoinductive properties of TCP. In vitro studies showed that SDF-1α/TCP scaffold significantly stimulated the expression of osteopontin and osteocalcin. As to the in vivo studies, the rabbit bone defect model showed that SDF-1α stimulated more new bone formation. In conclusion, SDF-1α/TCP bioceramic scaffolds could further promote bone regeneration compared to pure TCP bioceramics.

scholarly journals Biodegradable Zn–Sr alloy for bone regeneration in rat femoral condyle defect model: In vitro and in vivo studies

2021 ◽  
Vol 6 (6) ◽  
pp. 1588-1604
Author(s):  
Bo Jia ◽  
Hongtao Yang ◽  
Zechuan Zhang ◽  
Xinhua Qu ◽  
Xiufeng Jia ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Femoral Condyle ◽  
In Vivo Studies ◽  
Defect Model

scholarly journals Three-Dimensional Zirconia-Based Scaffolds for Load-Bearing Bone-Regeneration Applications: Prospects and Challenges

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3207
Author(s):  
Kumaresan Sakthiabirami ◽  
Vaiyapuri Soundharrajan ◽  
Jin-Ho Kang ◽  
Yunzhi Peter Yang ◽  
Sang-Won Park
Keyword(s):  
Bone Regeneration ◽  
Biological Activities ◽  
Three Dimensional ◽  
In Vivo Studies ◽  
High Mechanical Strength ◽  
Real World Applications ◽  
3D Fabrication ◽  
Dental Applications

The design of zirconia-based scaffolds using conventional techniques for bone-regeneration applications has been studied extensively. Similar to dental applications, the use of three-dimensional (3D) zirconia-based ceramics for bone tissue engineering (BTE) has recently attracted considerable attention because of their high mechanical strength and biocompatibility. However, techniques to fabricate zirconia-based scaffolds for bone regeneration are in a stage of infancy. Hence, the biological activities of zirconia-based ceramics for bone-regeneration applications have not been fully investigated, in contrast to the well-established calcium phosphate-based ceramics for bone-regeneration applications. This paper outlines recent research developments and challenges concerning numerous three-dimensional (3D) zirconia-based scaffolds and reviews the associated fundamental fabrication techniques, key 3D fabrication developments and practical encounters to identify the optimal 3D fabrication technique for obtaining 3D zirconia-based scaffolds suitable for real-world applications. This review mainly summarized the articles that focused on in vitro and in vivo studies along with the fundamental mechanical characterizations on the 3D zirconia-based scaffolds.

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

In-vitro and in-vivo studies of PLA / PCL / gelatin composite scaffold containing ascorbic acid for bone regeneration

2020 ◽  
pp. 102077 ◽  
Author(s):  
Seyedeh Fatemeh Hashemi ◽  
Mohsen Mehrabi ◽  
Arian Ehterami ◽  
Anneh Mohammad Gharravi ◽  
Fateme Sadat Bitaraf ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
In Vivo Studies

New Composite Material: PLLA and Tricalcium Phosphate for Orthopaedic Applications-In Vitro and In Vivo Studies (Part 1)

2016 ◽  
pp. 173-180
Author(s):  
Jean Charles Le Huec ◽  
Antonio Faundez ◽  
Stephane Aunoble ◽  
Rachid Sadikki ◽  
Julien Rigal
Keyword(s):  
Composite Material ◽  
Tricalcium Phosphate ◽  
In Vivo Studies

scholarly journals Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo

2019 ◽  
Vol 20 (7) ◽  
pp. 1572 ◽  
Author(s):  
Diego López Tenorio ◽  
Carlos Valencia ◽  
Cesar Valencia ◽  
Fabio Zuluaga ◽  
Mayra Valencia ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bone Regeneration ◽  
Wistar Rats ◽  
Biomechanical Properties ◽  
Cellular Membrane ◽  
In Vivo Studies ◽  
New Bone Formation ◽  
Critical Size Defects ◽  
Tissue Recovery

In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO and chitosan (CS) can not only improve the biomechanical properties of the scaffold but also generate a synergistic effect, facilitating tissue recovery. In vivo studies on GO are scarce; therefore, biocompatibility tests on CS-GO scaffolds and bone regeneration experiments on critical size defects were carried out on Wistar rats. Scaffolds made of CS, CS-GO 0.5%, and CS-GO 1% were prepared and implanted on Wistar rats cranial bones for three months. Scaffold samples were analyzed through histochemistry and scanning electron microscopy. The analysis performed showed reabsorption of the material by phagocytic activity and new bone formation. The CS-GO 0.5% formulation gave the best performance in bone regeneration, with excellent biocompatibility. These results show the potential of this compound for tissue regeneration opening and medical applications.

scholarly journals The Influence of Eggshell on Bone Regeneration in Preclinical In Vivo Studies

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 476
Author(s):  
Horia Opris ◽  
Cristian Dinu ◽  
Mihaela Baciut ◽  
Grigore Baciut ◽  
Ileana Mitre ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Animal Studies ◽  
Meta Analysis ◽  
Bone Matrix ◽  
In Vivo Studies ◽  
Defect Model ◽  
Exclusion Criteria ◽  
Freeze Dried ◽  
Grafting Materials

The aim of this study is to systemically review the available evidence on the in vivo behavior of eggshell as a guided bone regeneration substitute material. Five databases (PubMed, Cochrane, Web of Science, Scopus, EMBASE) were searched up to October 2020. In vivo animal studies with a bone defect model using eggshell as a grafting material were included. Risk of bias was assessed using SYRCLE tool and the quality assessment using the ARRIVE guidelines. Overall, a total of 581 studies were included in the study, 187 after duplicate removal. Using the inclusion and exclusion criteria 167 records were further excluded. The full text of the remaining 20 articles was assessed for eligibility and included in the qualitative and quantitative assessment synthesis. There were different methods of obtaining eggshell grafting materials. Eggshell is a biocompatible grafting material, with osteoconduction proprieties. It forms new bone similar to Bio-Oss and demineralized freeze-dried bone matrix. It can be combined with other materials to enhance its proprieties. Due to the high variability of the procedures, animals, production and assessment methods, no meta-analysis could be performed. Eggshell might be considered a promising biomaterial to be used in bone grafting procedures, though further research is needed.

Effect of Silicate Incorporation in Alpha-Tricalcium Phosphate on Behaviors of Osteoblast-Like Cells

2016 ◽  
Vol 720 ◽  
pp. 90-94
Author(s):  
Masanobu Kamitakahara ◽  
Takashi Shirato ◽  
Taishi Yokoi ◽  
Hideaki Matsubara ◽  
Yasuaki Shibata ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Proliferation And Differentiation ◽  
Porous Silicate

Silicate-containing alpha-tricalcium phosphate (α-TCP) ceramics are expected to be useful scaffolds for bone regeneration because α-TCP shows high biodegradability and silicate ions are expected to promote the bone formation. We previously revealed that the porous silicate-containing α-TCP granules provided earlier bone formation and showed lower biodegradability than the porous silicate-free α-TCP granules in vivo. In order to reveal the mechanism of the bone formation promoted by silicate incorporation, the proliferation and differentiation of osteoblast-like cells on the silicate-containing and silicate-free α-TCP ceramics were examined in vitro. The silicate incorporation in α-TCP promoted the differentiation of osteoblast-like cells, and it might be one of the factors to promote bone formation In Vivo.

The myelotoxicity of chloramphenicol: in vitro and in vivo studies: I. In vitro effects on cells in culture

1997 ◽  
Vol 16 (10) ◽  
pp. 570-576 ◽  
Author(s):  
DE Holt ◽  
TA Ryder ◽  
A. Fairbairn ◽  
R. Hurley ◽  
D. Harvey
Keyword(s):  
Progenitor Cells ◽  
Aplastic Anaemia ◽  
Monkey Kidney ◽  
In Vivo Studies ◽  
Patient Treatment ◽  
Morphological Effect ◽  
Dividing Cells ◽  
In Vitro Effects

1 Chloramphenicol is used extensively in non-industria lized countries for the treatment of life-threatening infections because it is cheap and effective, despite its known hemotoxicity and linkage to fatal aplastic anaemia. It is important to define the mechanism of toxicity so that means can be devised to ameliorate the toxic effects in order to produce safer usage. 2 Chloramphenicol, at concentrations from 5 mM to 2 mM initiated apoptosis in dividing cells from a monkey kidney-derived cell line and in haematopoie tic progenitor cells from human neonatal cord blood. 3 Growth of progenitor cells was suppressed at concen trations of chloramphenicol which would be consid ered less than therapeutic during patient treatment. 4 These effects could be ameliorated in progenitor cells by co-culture with the antioxidant mercaptoethyla mine and in monkey kidney cells by co-culture with vitamin C. 5 This is the first report of apoptosis in chloramphenicol toxicity and suggests a possible link between a metabolic event i.e. the production of free radicals; a morphological effect, apoptosis; and a clinical effect, bone marrow suppression and aplastic anaemia.

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