scholarly journals Combined Treatment of Adipose Derived-Mesenchymal Stem Cells and Pregabalin Is Superior to Monotherapy for the Treatment of Neuropathic Pain in Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Shimaa Mohammad Yousof ◽  
Doaa Attia ElSayed ◽  
Amani A. El-Baz ◽  
Hanaa S. Sallam ◽  
Faten Abbas
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Inflammatory Cytokine ◽  
Combined Treatment ◽  
Medial Gastrocnemius ◽  
Albino Rats ◽  
Cytokine Levels

Aims. Neuropathic pain following nerve injury does not respond well to most available pharmacological remedies. We aimed to compare the outcome of the addition of adipose-derived mesenchymal stem cells (ADMSCs) to pregabalin for neuropathic pain treatment. Methods. Adult female albino rats ( n = 100 ) were randomized to receive traumatic sciatic nerve injury or sham. Animals were then randomized to ADMSC treatment with or without pregabalin. We conducted a battery of neurobehavioral and electrophysiological to assess neuropathic pain. Following sacrifice, we evaluated the histological changes and gene expression of brain-derived neurotrophic factor (BDNF) in the sciatic nerve. Serum and sciatic nerve tissue pro- and inflammatory cytokine levels were also assessed. Results. (1) All treatments significantly improved thermal withdrawal latency, sciatic nerve conduction velocity, and proinflammatory cytokine levels in injured animals, with no significant effect of the combined treatments compared to pregabalin monotherapy ( p < 0.05 each). (2) Combined treatment significantly improved medial gastrocnemius electromyographic amplitude and sciatic function index compared to pregabalin monotherapy ( p < 0.05 each). (3) Combined treatment significantly increased the BDNF expression, decreased anti-inflammatory cytokine ( p < 0.05 each), and restored the structural nerve damage, compared to pregabalin monotherapy. Conclusions. Combined treatment is associated with greater improvement of the sciatic nerve structure and function. Further studies are warranted to study the mechanism of action of the combined treatment to improve neuropathic pain.

Mesenchymal Stem Cells in a Polycaprolactone Conduit Promote Sciatic Nerve Regeneration and Sensory Neuron Survival after Nerve Injury

2012 ◽  
Vol 18 (19-20) ◽  
pp. 2030-2039 ◽  
Author(s):  
Flávia Frattini ◽  
Fatima Rosalina Pereira Lopes ◽  
Fernanda Martins Almeida ◽  
Rafaela Fintelman Rodrigues ◽  
Leonardo Cunha Boldrini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Sensory Neuron ◽  
Neuron Survival ◽  
Sciatic Nerve Regeneration

Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells

Biomaterials ◽  
2017 ◽  
Vol 142 ◽  
pp. 90-100 ◽  
Author(s):  
Wei Zhang ◽  
Lihai Zhang ◽  
Jianheng Liu ◽  
Licheng Zhang ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Nerve Conduit

Differentiated Mesenchymal Stem Cells for Sciatic Nerve Injury

2011 ◽  
Vol 7 (3) ◽  
pp. 664-671 ◽  
Author(s):  
Michal Dadon-Nachum ◽  
Ofer Sadan ◽  
Itay Srugo ◽  
Eldad Melamed ◽  
Daniel Offen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury

scholarly journals Molecular, Immunomodulatory, and Histopathological Role of Mesenchymal Stem Cells and Beetroot Extract on Cisplatin Induced Testicular Damage in Albino Rats

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1142
Author(s):  
Marwa T. Hassen ◽  
Hanaa K. Mohamed ◽  
Metwally M. Montaser ◽  
Mohamed E. El-Sharnouby ◽  
Nabil Awad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sperm Count ◽  
Combined Treatment ◽  
Regulatory Protein ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Albino Rats ◽  
Testicular Toxicity ◽  
Testicular Damage

Cisplatin (Cis) a drug commonly used as a chemotherapeutic agent to treat various types of cancer, inducing testicular damage. The present study aimed to investigate the inhibitory potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and beetroot extract (BRE) in albino rats after testicular toxicity induced by cisplatin. Thirty adult male albino rats were grouped into: the control group, Cis group receiving a single dose of 7 mg/kg i.p. (intraperitoneal) to induce testicular toxicity, Cis plus BM-MSCs injected Cis followed by 2 × 106 of BM-MSCs; Cis plus BRE group receiving Cis followed by 300 mg/kg body weight/day of BRE, and Cis plus BM-MSCs and BRE group. In the current study, Cis reduced sperm count, serum testosterone level, and testicular activity of alkaline phosphatase (AKP), besides a marked inhibition of succinate dehydrogenase (SDH) activity. In addition, it significantly increased malondialdehyde (MDA) and along with a marked decrease in testis reduced glutathione content and total antioxidant capacity (TAC). At the same time, Cis administration resulted in a marked elevation in interleukine-6 and the iNOS and caspase-3 genes; however, it decreased the expression of steroidogenic acute regulatory protein (StAR). Combined treatment with BM-MSCs and BRE resulted in great improvement of all previous parameters. These results were also confirmed by histopathological and immunohistochemical examination. In conclusion, both MSCs and BRE were found to have potent potentials to inhibit testicular damage induced by cisplatin.

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