scholarly journals Comprehensive Quality Evaluation of American Ginseng for Different Parts and Abnormal Trait Based on the Major Ginsenoside Contents and Morphological Characteristics

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jingping Yu ◽  
Tong Xu ◽  
Haiyan Lin ◽  
Ying Lin ◽  
Jie Zhou ◽  
...  
Keyword(s):  
High Performance ◽  
Economic Value ◽  
Morphological Characteristics ◽  
Total Content ◽  
American Ginseng ◽  
Root Diameter ◽  
Main Root ◽  
Fibrous Root ◽  
Ginsenoside Content ◽  
Different Parts

The demand for American ginseng, a famous traditional medicine and high-grade healthy food, has increased dramatically over recent years. However, only the main root is popular among consumers, whereas other parts of American ginseng are rarely available in the market. In this study, the contents of 5 major ginsenosides (Re, Rc, Rg1, Rd, and Rb1) were determined through high-performance liquid chromatography. Our study showed that all these 5 major ginsenosides are found in different parts of American ginseng plants, and the total content in different parts varied significantly in the following order: fibrous   root > flower > branch   root > main   root > leaf > stem . Interestingly, the total content in the fibrous root was approximately 2.24 times higher than that in the main root. Further research indicated that the ginsenoside content in American ginseng with abnormal characteristics (physical deformity caused by disease and discolouration) is similar to that in the normal plant. Interestingly, a positive correlation was observed between the main root diameter and total ginsenoside content, whereas a negative correlation was observed between the main root length and total ginsenoside content. Our comprehensive study revealed that all parts of American ginseng, including the main root with abnormal characteristics, possess medicinal or economic value. Therefore, our results provide feasible evidence to further explore the potential application of American ginseng.

scholarly journals Comprehensive Investigation on Ginsenosides in Different Parts of a Garden-Cultivated Ginseng Root and Rhizome

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1696
Author(s):  
Junqian Pan ◽  
Wei Zheng ◽  
Xu Pang ◽  
Jie Zhang ◽  
Xiaojuan Chen ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Relative Content ◽  
Ginseng Root ◽  
Main Root ◽  
Fibrous Root ◽  
Flight Mass Spectrometry ◽  
Charged Aerosol ◽  
Different Parts

Background: Ginseng is widely used as herb or food. Different parts of ginseng have diverse usages. However, the comprehensive analysis on the ginsenosides in different parts of ginseng root is scarce. Methods: An ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) combined with UNIFI informatics platform and ultra-high-performance liquid chromatography-charged aerosol detection (UHPLC-CAD) were employed to evaluate the different parts of cultivated ginseng root. Results: 105 ginsenosides including 16 new compounds were identified or tentatively characterized. 22 potential chemical markers were identified, 20, 17, and 19 for main root (MR) and fibrous root (FR), main root (MR) and branch root (BR), and main root (MR) and rhizome (RH), respectively. The relative contents of Re, Rb1, 20(R)-Rh1, Rd, and Rf were highest in FR. The relative content of Rg1 was highest in RH. The total relative content of pharmacopoeia indicators Rg1, Re, and Rb1 was highest in FR. Conclusion: The differences among these parts were the compositions and relative contents of ginsenosides. Under our research conditions, the peak area ratio of Rg1 and Re could distinguish the MR and FR samples. Fibrous roots showed rich ingredients and high ginsenosides contents which should be further utilized.

scholarly journals Quality Analysis of American Ginseng Cultivated in Heilongjiang Using UPLC-ESI−-MRM-MS with Chemometric Methods

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2396 ◽  
Author(s):  
Yong-Gang Xia ◽  
Yan Song ◽  
Jun Liang ◽  
Xin-Dong Guo ◽  
Bing-You Yang ◽  
...  
Keyword(s):  
Hairy Roots ◽  
High Performance ◽  
Raw Materials ◽  
Multiple Reaction Monitoring ◽  
Simultaneous Detection ◽  
Total Content ◽  
American Ginseng ◽  
Quality Analysis ◽  
Panax Quinquefolium ◽  
Chemometric Methods

American ginseng (Panax quinquefolium) has long been cultivated in China for the function food and medicine. Here, ultra-high performance liquid chromatography was coupled with electrospray ionization and triple quadrupole mass spectrometry (UPLC-ESI−-TQ-MS) for simultaneous detection of 22 ginsenosides in American ginseng cultivated in Mudanjiang district of Heilongjiang. The extraction conditions also were optimized by a Box Behnken design experiment. The optimized result was 31.8 mL/g as ratio of liquid to raw materials, 20.3 min of extraction time, and 235.0 W of extraction powers. The quantitative MS parameters for these 22 compounds were rapidly optimized by single factor experiments employing UPLC-ESI−-multiple reaction monitoring or multiple ion monitoring (MRM/MIM) scans. Furthermore, the established UPLC-ESI−-MRM-MS method showed good linear relationships (R2 > 0.99), repeatability (RSD < 3.86%), precision (RSD < 2.74%), and recovery (94–104%). This method determined 22 bioactive ginsenosides in different parts of the plant (main roots, hairy roots, rhizomes, leaves, and stems) and growth years (one year to four years) of P. quinquefolium. The highest total content of the 22 analytes was in the hairy roots (1.3 × 105 µg/g) followed by rhizomes (7.1 × 104 µg/g), main roots (6.5 × 104 µg/g), leaves (4.2 × 104 µg/g), and stems (2.4 × 104 µg/g). Finally, chemometric methods, hierarchical clustering analysis (HCA) and partial least squares discrimination analysis (PLS-DA), were successfully used to classify and differentiate American ginseng attributed to different growth years. The proposed UPLC-ESI−-MRM-MS coupled with HCA and PLS-DA methods was elucidated to be a simple and reliable method for quality evaluation of American ginseng.

scholarly journals Determination of Ginsenoside Content in Asian and North American Ginseng Raw Materials and Finished Products by High-Performance Liquid Chromatography: Single-Laboratory Validation

2011 ◽  
Vol 94 (5) ◽  
pp. 1391-1399 ◽  
Author(s):  
Paula N Brown
Keyword(s):  
North American ◽  
High Performance ◽  
Raw Materials ◽  
American Ginseng ◽  
Coefficient Of Determination ◽  
Aqueous Methanol ◽  
Rp Hplc ◽  
Ginsenoside Content ◽  
Single Laboratory

Abstract A single-laboratory validation study was conducted for the quantification of Rg1, Re, Rb1, Rc, Rb2, and Rd in Asian ginseng (Panax ginseng C.A. Meyer) and North American ginseng (Panax quinquefolius L.) raw materials and finished products by RP-HPLC. The extraction with aqueous methanol was optimized for whole root, powdered extract, and finished product (raw, tablet, and capsule matrixes) test articles. Root materials were treated with base to hydrolyze acidic malonyl ginsenosides to their neutral counterparts. Calibration curves for each ginsenoside were linear over the following ranges (μg/g): 5–394 for Rg1, 15–1188 for Re, 39–2981 for Rb1, 6–499 for Rc, 5–406 for Rb2, and 7–600 for Rd, all having a coefficient of determination (r2) of ≥99.5%. The LOD for Rg1, Re, Rb1, Rc, Rb2, and Rd was determined to be 1.06, 1.25, 2.19, 1.24, 1.27, and 1.70 μg/mL, respectively. Quantitative determinations performed with eight test materials by two analysts over 3 days (n = 12) resulted in RSDr values that ranged from 1.11 to 7.61%.

scholarly journals Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of Trichosanthes kirilowii Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1440 ◽  
Author(s):  
Huang-qin Zhang ◽  
Pei Liu ◽  
Jin-ao Duan ◽  
Ling Dong ◽  
Er-xin Shang ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Root Bark ◽  
Main Root ◽  
Evaporative Light Scattering Detector ◽  
Evaporative Light Scattering ◽  
Trichosanthes Kirilowii ◽  
Different Parts

Trichosanthes kirilowii Maxim. is one of the original plants for traditional Chinese medicines Trichosanthis Fructus, Trichosanthis Semen, Trichosanthis Pericarpium and Trichosanthis Radix. Amino acids, nucleosides and carbohydrates are usually considered to have nutritional value and health-care efficacy. In this study, methods involving high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD), UV-visible spectrophotometry and ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) were established for quantifying carbohydrates (fructose, glucose, stachyose, raffinose and polysaccharide), fourteen nucleosides and twenty one amino acids. Moreover, sixty-three samples from nine different parts, including pericarp, seed, fruit pulp, stem, leaf, main root, main root bark, lateral root and lateral root bark of T. kirilowii from different cultivated varieties were examined. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. The results showed that the average content of total amino acids in roots (15.39 mg/g) and root barks (16.38 mg/g) were relatively higher than for others. Contents of nucleosides in all parts of T. kirilowii were below 1.5 mg/g. For carbohydrates, fruit pulp has a higher content than others for glucose (22.91%), fructose (20.63%) and polysaccharides (27.29%). By using partial least-squared discriminate analysis (PLS-DA), Variable importance in the projection (VIP) plots and analysis of variance (ANOVA) analysis, the characteristic components of the different organs (fruit, stems and leaves, roots) were found. This analysis suggested there were potential medicinal and nutritive health care values in various parts of the T. kirilowii, which provided valuable information for the development and utilization of T. kirilowii.

scholarly journals Investigation of Insulation Characteristics of GFRP Crossarm Subjected to Lightning Transient

Energies ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 4386
Author(s):  
Muhammad Syahmi Abd Rahman ◽  
Mohd Zainal Abidin Ab Kadir ◽  
Muhamad Safwan Abd Rahman ◽  
Miszaina Osman ◽  
Shamsul Fahmi Mohd Nor ◽  
...  
Keyword(s):  
High Performance ◽  
Breakdown Strength ◽  
Fiber Reinforced Polymer ◽  
The Finite Element Method ◽  
Glass Fiber Reinforced Polymer ◽  
Material Technology ◽  
Reinforced Polymer ◽  
Glass Fiber Reinforced ◽  
Maximum Field ◽  
Different Parts

The advancement of material technology has contributed to the variation of high-performance composites with good electrical insulation and mechanical properties. Their usage in electrical applications has grown since then. In Malaysia, the composite made of Glass Fiber Reinforced Polymer (GFRP) has been adopted for crossarm manufacturing and has successfully served 275 kV lines for a few decades. However, the combination of extreme conditions such as lightning transient and tropical climate can impose threats to the material. These issues have become major topics of discussion among the utilities in the Southeast Asian (SEA) region, and also in previous research. In Malaysia, more than 50% of total interruptions were caused by lightning. Limited studies can be found on the composite crossarm, especially on the square tube GFRP filled crossarm used in Malaysia. Therefore, this paper proposes to study the behavior of the particular GFRP crossarm, by means of its insulation characteristics. Experimental and simulation approaches are used. Throughout the study, the GFRP specimen is known to have an average breakdown strength at 7.2 kV/mm. In addition, the CFO voltages of the crossarm at different lengths are presented, whereby the behavior under dry and wet conditions is comparably discussed. At the same time, the polarity effect on the CFO voltages is highlighted. The maximum E-fields at the immediate moment before breakdown are analyzed by adopting the finite element method (FEM). Non-uniform distribution of E-fields is witnessed at different parts of the crossarm structure. Simultaneously, the maximum field localized on the crossarm immediately before the breakdown is also presented.

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

Comparison of the fast-start performances of closely related, morphologically distinct threespine sticklebacks (Gasterosteus spp.)

1996 ◽  
Vol 199 (12) ◽  
pp. 2595-2604 ◽  
Author(s):  
T Law ◽  
R Blake
Keyword(s):  
Angular Velocity ◽  
High Speed ◽  
High Performance ◽  
Gasterosteus Aculeatus ◽  
Morphological Characteristics ◽  
Maximum Velocity ◽  
Threespine Stickleback ◽  
Biomechanical Study ◽  
Maximum Acceleration ◽  
Fast Start

Fast-start escape performances for two species of threespine stickleback, Gasterosteus spp., were investigated using high-speed cinematography (400 Hz). The two fishes (not yet formally described, referred to here as benthic and limnetic) inhabit different niches within Paxton Lake, British Columbia, Canada, and are recent, morphologically distinct species. All escape responses observed for both species were double-bend C-type fast-starts. There were no significant differences between the species for any linear or angular parameter (pooled averages, both species: duration 0.048 s, distance 0.033 m, maximum velocity 1.10 m s-1, maximum acceleration 137 m s-2, maximum horizontal angular velocity 473.6 rad s-1 and maximum overall angular velocity 511.1 rad s-1). Benthics and limnetics have the greatest added mass (Ma) at 0.3 and 0.6 body lengths, respectively. The maximum Ma does not include the fins for benthics, but for limnetics the dorsal and anal fins contribute greatly to the maximum Ma. The deep, posteriorly placed fins of limnetics enable them to have a fast-start performance equivalent to that of the deeper-bodied benthics. Both the limnetic and benthic fishes have significantly higher escape fast-start velocities than their ancestral form, the anadromous threespine stickleback Gasterosteus aculeatus, suggesting that the high performance of the Paxton Lake sticklebacks is an evolutionarily derived trait. In this biomechanical study of functional morphology, we demonstrate that similar high fast-start performance can be achieved by different suites of morphological characteristics and suggest that predation might be the selective force for the high escape performance in these two fishes.

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