scholarly journals Comparison of 18F-FDG, 18F-Fluoroacetate, and 18F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Chun-Yi Wu ◽  
Hsin-Hua Hsieh ◽  
Pei-An Chu ◽  
Wen-Hsiang Hong ◽  
Ting-Yu Chang ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Fibrosis ◽  
Rat Model ◽  
Diagnostic Methods ◽  
Male Rats ◽  
Sprague Dawley ◽  
Positron Emission ◽  
Duct Ligation ◽  
Before And After ◽  
18F Fdg

Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [18F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([18F]FEPPA) ( n = 3 ), [18F]fluoroacetate ([18F]FAc) ( n = 3 ), and 18F-fluoro-2-deoxy-D-glucose ([18F]FDG) ( n = 3 ) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 ( n = 3 ), 1 ( n = 3 ), 2 ( n = 3 ), and 3 ( n = 3 ) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [18F]FEPPA and [18F]FDG, there was a significantly higher uptake in the liver after BDL (both P < 0.05 ), which lasted until week 2. However, the uptake of [18F]FAc in the liver was not significantly different before and after BDL ( P = 0.28 ). Collectively, both [18F]FEPPA and [18F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [18F]FAc is not recommended to diagnose liver fibrosis.

scholarly journals Amniotic Membrane Application Reduces Liver Fibrosis in a Bile Duct Ligation Rat Model

2011 ◽  
Vol 20 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Luciana B. Sant'anna ◽  
Anna Cargnoni ◽  
Lorenzo Ressel ◽  
Graziella Vanosi ◽  
Ornella Parolini
Keyword(s):  
Bile Duct ◽  
Liver Fibrosis ◽  
Amniotic Membrane ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Duct Ligation

Aqueous extract of Artemisia iwayomogi Kitamura attenuates cholestatic liver fibrosis in a rat model of bile duct ligation

2012 ◽  
Vol 50 (10) ◽  
pp. 3505-3513 ◽  
Author(s):  
Jong-Min Han ◽  
Hyeong-Geug Kim ◽  
Min-Kyung Choi ◽  
Jin-Suk Lee ◽  
Hye-Jung Park ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Fibrosis ◽  
Aqueous Extract ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Artemisia Iwayomogi ◽  
Duct Ligation

Evidence that bile salts are important for iron absorption

1994 ◽  
Vol 266 (2) ◽  
pp. G318-G323 ◽  
Author(s):  
A. J. Sanyal ◽  
J. I. Hirsch ◽  
E. W. Moore
Keyword(s):  
Bile Duct ◽  
Bile Salts ◽  
Bile Duct Ligation ◽  
Whole Body ◽  
Sprague Dawley ◽  
Oh Groups ◽  
Group 4 ◽  
Duct Ligation ◽  
Before And After ◽  
Group 1

The role of bile salts in intestinal Fe absorption has not previously been defined. We have recently shown that bile salts having cholanic ring 7 alpha-OH and/or 12 alpha-OH groups bind Fe2+ with high affinity at premicellar concentrations and also produce a two-to threefold enhancement in Fe2+ uptake from perfused intestinal segments in vivo. However, the physiological relevance of these observations for Fe2+ absorption was not known. To study this, we examined the effects of a single dose of taurocholate (TC) administered with 59Fe-FeSO4 in reversing iron malabsorption induced by bile duct ligation. Fe absorption from three 0.5-mg doses of 59Fe-FeSO4 was measured from five groups of six adult Sprague-Dawley rats each: group 1, before and after bile duct ligation and subsequent administration of 59Fe-FeSO4 + TC test dose; group 2, before and after bile duct ligation followed by a third dose of Fe alone; group 3, before and after bile duct ligation followed by a third dose of Fe+taurodehydrocholate (TDHC); group 4, sham laparotomy; and group 5, nonoperated controls where Fe absorption was measured from three doses of 59Fe-FeSO4 at the same time intervals as group 1. Absorption was measured, using whole body counting, after achievement of steady-state counts after each Fe dose. Mean percentage absorption from dose 1 (baseline) was 35.33 +/- 4.6% (SE). No significant differences between any groups were noted. In nonoperated controls (group 4), Fe absorption was virtually identical after all three doses. Also, hematocrit did not change in any of the 30 animals.(ABSTRACT TRUNCATED AT 250 WORDS)

CGX, a multiple herbal drug, improves cholestatic liver fibrosis in a bile duct ligation-induced rat model

2013 ◽  
Vol 145 (2) ◽  
pp. 653-662 ◽  
Author(s):  
Hyeong-Geug Kim ◽  
Jong-Min Han ◽  
Hye-Won Lee ◽  
Jin-Seok Lee ◽  
Seung-Wan Son ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Fibrosis ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Herbal Drug ◽  
Duct Ligation

scholarly journals 3D molecular MR imaging of liver fibrosis and response to rapamycin therapy in a bile duct ligation rat model

2015 ◽  
Vol 63 (3) ◽  
pp. 689-696 ◽  
Author(s):  
Christian T. Farrar ◽  
Danielle K. DePeralta ◽  
Helen Day ◽  
Tyson A. Rietz ◽  
Lan Wei ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Fibrosis ◽  
Mr Imaging ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Duct Ligation

Adenoviral CCN3/NOV gene transfer fails to mitigate liver fibrosis in an experimental bile duct ligation model because of hepatocyte apoptosis

2012 ◽  
Vol 32 (9) ◽  
pp. 1342-1353 ◽  
Author(s):  
Erawan Borkham-Kamphorst ◽  
Sebastian Huss ◽  
Eddy Leur ◽  
Ute Haas ◽  
Ralf Weiskirchen
Keyword(s):  
Bile Duct ◽  
Gene Transfer ◽  
Liver Fibrosis ◽  
Bile Duct Ligation ◽  
Hepatocyte Apoptosis ◽  
Duct Ligation

scholarly journals Kidney Injury Caused by Preeclamptic Pregnancy Recovers Postpartum in a Transgenic Rat Model

2021 ◽  
Vol 22 (7) ◽  
pp. 3762
Author(s):  
Sarah M. Kedziora ◽  
Kristin Kräker ◽  
Lajos Markó ◽  
Julia Binder ◽  
Meryam Sugulle ◽  
...  
Keyword(s):  
Rat Model ◽  
Renal Damage ◽  
Kidney Injury ◽  
Tissue Growth ◽  
Female Rats ◽  
Male Rats ◽  
Renal Diseases ◽  
Transgenic Rat ◽  
Increased Risk ◽  
Before And After

Preeclampsia (PE) is characterized by the onset of hypertension (≥140/90 mmHg) and presence of proteinuria (>300 mg/L/24 h urine) or other maternal organ dysfunctions. During human PE, renal injuries have been observed. Some studies suggest that women with PE diagnosis have an increased risk to develop renal diseases later in life. However, in human studies PE as a single cause of this development cannot be investigated. Here, we aimed to investigate the effect of PE on postpartum renal damage in an established transgenic PE rat model. Female rats harboring the human-angiotensinogen gene develop a preeclamptic phenotype after mating with male rats harboring the human-renin gene, but are normotensive before and after pregnancy. During pregnancy PE rats developed mild tubular and glomerular changes assessed by histologic analysis, increased gene expression of renal damage markers such as kidney injury marker 1 and connective-tissue growth factor, and albuminuria compared to female wild-type rats (WT). However, four weeks postpartum, most PE-related renal pathologies were absent, including albuminuria and elevated biomarker expression. Only mild enlargement of the glomerular tuft could be detected. Overall, the glomerular and tubular function were affected during pregnancy in the transgenic PE rat. However, almost all these pathologies observed during PE recovered postpartum.

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