scholarly journals In Vivo Antiplasmodial Activity and Toxicological Analyses of the Ethanolic Leaf and Twig Extract of Faurea speciosa Welw. (Proteaceae)

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Felix Ayisi ◽  
Caleb Nketia Mensah ◽  
Lawrence Sheringham Borquaye
Keyword(s):  
Acute Toxicity ◽  
Lethal Dose ◽  
Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Plant Samples ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

In Africa, medicinal plants are commonly used to treat malaria and other diseased conditions. The ethanolic leaf and twig extract of Faurea speciosa has been shown to possess promising antiplasmodial properties. This present study was aimed at investigating its antiplasmodial effect in vivo. Qualitative phytochemical screening was carried out on the plant samples using standard methods. The antiplasmodial effect against early infection, curative effect against established infection, and prophylactic effect against residual infection were studied in vivo in Plasmodium berghei-infected mice while the carrageenan-induced edema model in chicks was used for anti-inflammatory tests. The phosphomolybdenum and DPPH radical scavenging assays were used in the evaluation of antioxidant potential. Acute toxicity of the extract was evaluated using the Organization for Economic Cooperation and Development (OECD) guidelines. Phytochemical screening of plant samples revealed the presence of flavonoids, coumarins, tannins, saponins, and glycosides. Faurea speciosa leaf and twig extract exhibited significant antiplasmodial activities in the mouse model with parasite suppression rates of 66.63%, 71.70%, and 56.93% in the suppressive, curative, and prophylactic tests, respectively. A 55.50% reduction of edema in the anti-inflammatory test indicated moderate success in reducing inflammation. The total antioxidant capacity of the extract was determined to be 65.4 mg AAE/g of extract, while in the DPPH radical scavenging assay, the IC50 value was found to be 499.4 μg/mL. With the exception of an inconsistent rise in urea level, there was no significant difference in the other biochemistry parameters in the acute toxicity studied. The median lethal dose (LD50) of the extract was over 2000 mg/kg. The results of this study show that Faurea speciosa leaf and twig extract has promising antimalarial capabilities and is fairly safe at low concentrations.

scholarly journals In vitro Antioxidant Activity and Inhibition of Fe2+ and SNP Lipid Peroxidation of African Mistletoes (Tapinanthus globiferus) from Three Selected Host Plants in Jos Plateau State Nigeria

2019 ◽  
pp. 1-10
Author(s):  
Jane-Rose I. Oche ◽  
Titilayo O. Johnson ◽  
Augustina O. Akinsanmi ◽  
Kiri H. Jaryum ◽  
Timothy Francis
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Psidium Guajava ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Vernonia Amygdalina ◽  
Significant Difference ◽  
Dpph Radical Scavenging

Aim: The aim of this study was to investigate and compare the antioxidative properties of the mistletoe plant obtained from three different host species namely Psidium guajava, Vernonia amygdalina and Moringa olifera lam. Study Design: Experimental Design Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences and Department of Biochemistry, College of Health Sciences, University of Jos, Nigeria. Methodology: Crude methanolic leaf extracts were studied for their antioxidative properties; Iron reducing and Iron-chelating activities, Nitric oxide (NO) radical and 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging activities and the lipid peroxidation and thiobarbituric acid reaction (TBAR) methods. One way ANOVA was used for the result analysis with P<.05 for significant difference. Results: Mistletoes from Psidum guajava (MSPG) had significantly higher reducing property (0.16 – 0.20mg/mL); the chelating property of Mistletoes from Moringa olifera (MSMO) was significantly lower (45.7 – 58.9%); DPPH radical scavenging activity had no significant difference; and Nitric oxide scavenging activity was significantly higher in MSPG (72.1% in 75mg/mL) than the extracts from other hosts. MSPG had significantly higher TBAR inhibition using both FeSO4 (77.8% at 125µg/mL) and Sodium nitroprusside (61.6+1.0% at 125µg/mL) with an IC50 of 30.27µg/mL . Extract of Tapinanthus globiferus leaves from Psidium guajava had more antioxidative activities in the TBARs followed by Tapinanthus globiferus leaf extract from Vernonia amygdalina (MSVA). Conclusion: From the study, mistletoes from Psidium guajava had higher antioxidant activity compared to other hosts, which probably justifies its use for treatment of cancer in traditional medicinal practice.

scholarly journals Antioxidant and Anti-inflammatory Activities of Cynaroside from Elsholtiza bodinieri

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Ying Zou ◽  
Min Zhang ◽  
Tingrui Zhang ◽  
Junwen Wu ◽  
Jun Wang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Reducing Power ◽  
Total Content ◽  
Preparative Hplc ◽  
Dpph Radical ◽  
Anti Inflammatory ◽  
Flavonoid Fraction ◽  
Reducing Power Assay

The flavonoid fraction was obtained from Elsholtiza bodinieri Vaniot (EBV) by ethanol-reflux and liquid-liquid extraction. The total content of flavonoid was 179.55 mg/g, and the purity was 64.6%. Then cynaroside with the purity of 94% was isolated from the fraction by preparative HPLC and characterized by the combined usage of HPLC, ESI-MS, and NMR. The antioxidant activity of cynaroside was determined using 2 complementary methods, namely, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assay. The anti-inflammatory effect of cynaroside was investigated based on in-vitro and in-vivo experiment. The results showed that cynaroside from EBV scavenged DPPH radical and reduced Fe3+ to Fe2+ effectively, inhibited NO and ROS production in LPS-stimulated RAW264.7 cells and attenuated the inflammation in the mouse model significantly ( p < 0.01), which showed it to be a nutraceutical product in the food industry.

scholarly journals Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalum crepidioides (Bent) Leaf Cold and Hot Water Extracts

2019 ◽  
pp. 1-12 ◽  
Author(s):  
B. A. Akinpelu ◽  
A. Godwin ◽  
T. Gbadegesin ◽  
N. Ajakaye ◽  
S. E. Omotosho ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Radical Scavenging ◽  
Hot Water ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Aim: To investigate the anti-inflammatory, anti-oxidant and genotoxicity activities of Crassocephalum crepidioides leaf. Study Design: Comparative investigations of the medicinal value and toxicity profile of cold water (CW) and hot water (HW) extracts of C. crepidioides leaf. Place and Duration of Study: Biochemistry and Molecular Biology Department, Obafemi Awolowo University, Ile-Ife. January 2015-October 2016. Materials and Methods: CW and HW of C. crepidioides were analyzed for anti-inflammatory activity via red blood cell membrane stabilization technique and in vitro methods using DPPH radical scavenging activity, thiobarbituric acid-reactive substances (TBARS), ferric reducing antioxidant power (FRAP) and inhibition of oxidative haemolysis were employed to evaluate the antioxidant property. Allium cepa chromosomal assay was adopted to investigate the genotoxic effect of the extracts. Total flavonoid and phenolic contents of the extracts were estimated spectrophotometrically. Results: Both extracts stabilized stressed red blood cell membranes with maximum percentage stability of 50.97±0.06 and 90.90±0.02 at 0.5 and 2.0 mg/ml for CW and HW extracts respectively. The CW extract elicited no DPPH radical scavenging (IC50 -0.63±0.02 mg/ml) and lipid peroxidation (IC50 -0.32±0.00) activities. HW extract had IC50 of 0.29±0.02 and 0.17±0.00 mg/ml for DPPH and lipid peroxidation. CW and HW extracts exhibited FRAP activity of 1186.96±0.01 and 1015.54±0.01 µmol AAE/g respectively. CW extract displayed a weaker protection (29.01±0.01%) against oxidative haemolysis compared to HW extract (68.70 ± 0.00%). CW extract contained higher phenolic contents (2.16±0.03 µmolGAE/g extract) while the HW extract contained higher flavonoids (0.61±0.05 µmolQE/g extract). CW and HW extracts inhibited A. cepa root growth to 71.40±0.02 and 59.10±0.02% respectively. A. cepa mitotic index was reduced to 8.85±0.01 and 8.67±0.02 for CW and HW extracts as compared with control (26.62%). Conclusion: The study concluded that consumption of C. crepidioides leaf in cooked form has more medicinal values however, both CW and HW extracts are capable of causing cellular damage at high doses.

scholarly journals In vitro anti-inflammatory, antioxidant and qualitative phytochemical evaluation of the Phytexponent preparation of selected plants

2020 ◽  
Author(s):  
Gervason Moriasi ◽  
Elias Nelson ◽  
Epaphrodite Twahirwa
Keyword(s):  
Oxidative Stress ◽  
Protein Denaturation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Scientific Data ◽  
Etiologic Factor ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Abstract Oxidative stress is a critical etiologic factor and driver of inflammatory responses, witnessed in chronic and persistent conditions. The current anti-oxidative stress and anti-inflammatory drugs are associated with detrimental effects, high dependence, high costs, inaccessibility, among other drawbacks; therefore, a need for alternatives is imperative. Despite the remarkable potential of medicinal plants, there are scanty empirical studies on their pharmacologic efficacy. The Phytexponent is an alcoholic polyherbal preparation of Allium sativum, Triticum repens, Echinacea purpurea, Viola tricolor and Matricaria chamomilla. In complementary medicine, the Phytexponent is used to boost immunity, to treat inflammatory disorders, oxidative stress, blood pressure, diabetes, stress/depression, among other conditions. However, there is no sufficient scientific data to support these healing claims. Therefore, in the current study evaluated the in vitro anti-inflammatory, antioxidant activities and qualitative phytochemical composition of the Phytexponent. The in vitro anti-inflammatory activities were evaluated using the inhibition of protein denaturation and the human erythrocyte (HRBC) membrane stabilization techniques. Antioxidant activities were evaluated by the 1,1-diphenyl-picryl-1-hydrazyl (DPPH) radical scavenging-, the hydroxyl radical scavenging- and catalase activities. Qualitative phytochemical screening was performed using standard procedures. The results showed a significantly higher percentage inhibition of heat-induced- and hypotonicity induced HRBC hemolysis by the Phytexponent at concentrations of 50 % and 100 %, compared with the percentage inhibitions of etanercept (p<0.05). No significant differences in percentage inhibitions of protein denaturation were observed among concentrations of 12.5 %,25.0 %,50.0 %,100.0 % of the Phytexponent and etanercept (25 mg/ml) (p˃0.05). Furthermore, the Phytexponent demonstrated high antioxidant activities against the DPPH- (IC50=0.00733%) and the hydroxyl- (IC50 = 0.716 %) radicals in vitro.The Phytexponent recorded significantly higher catalase activities at concentrations of 1 % and 0.1 % than those recorded by ascorbic acid at similar concentrations. Qualitative phytochemical screening revealed the presence of phenols, flavonoids, tannins, among other antioxidant associated phytochemicals. The bioactivities of the Phytexponent reported herein, were attributed to the presence of these phytochemicals. Further studies to establish specific mode(s) through which the Phytexponent exerts in vitro anti-inflammatory and antioxidant effects are encouraged. Moreover, in vivo anti-inflammatory and antioxidant activities should be done to determine the replicability of these findings in vivo. Bioassay-guided isolation of compounds responsible for the reported bioactivities herein should be done.

scholarly journals Phytochemical Screening and Evaluation of Antimicrobial and Antioxidant Activity of Mahonia napaulensis (Jamanemandro) Bark Extract

2021 ◽  
Vol 19 (2) ◽  
pp. 55-61
Author(s):  
Ranjan Paudel ◽  
Rabi Kiran Sharma ◽  
Shreeya Bhandari ◽  
Manan Koirala ◽  
Ganesh Bhandari ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Mahonia napaulensis also known as “Jamanemandro” in Nepali is a medium-sized stiff evergreen shrub widely distributed in South East Asia at an altitude of 1000-2000m, is traditionally used to treat dysentery and eye inflammation. This research focuses on screening of the phytochemicals, antimicrobial, and antioxidant properties of this plant. The methanolic extract was prepared using a Soxhlet apparatus. The antioxidant properties of extract were carried out by 50% inhibitory concentration (IC50) values from 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. The phytochemical screening confirmed the presence of terpenoids, reducing sugars, tannins, alkaloids, glycosides including cardiac glycosides and steroids. The antimicrobial activity was studied using the disc diffusion method in five different human pathogens named Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Shigella spps. The methanol extract was compared with standard chloramphenicol and showed that methanolic extract of is M.napaulensis exhibit maximum effect against S. aureus with higher growth inhibition zones (27.3 mm), followed by P. aeruginosa, Shigella spps., E. coli and S. typhi. These antimicrobial properties showed similar effect to that of positive control, chloramphenicol. The IC50 values from DPPH radical scavenging were 230.89 µg/mL and 182.73 µg/mL of methanol extract and ascorbic acid, respectively. Due to this antimicrobial and antioxidant properties of M. napaulensis it was widely applicable in biomedical field.

scholarly journals In vitro anti-inflammatory, antioxidant and qualitative phytochemical evaluation of the Phytexponent preparation of selected plants

2020 ◽  
Author(s):  
Gervason Moriasi ◽  
Elias Nelson ◽  
Epaphrodite Twahirwa
Keyword(s):  
Oxidative Stress ◽  
Protein Denaturation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Scientific Data ◽  
Etiologic Factor ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Abstract Oxidative stress is a critical etiologic factor and driver of inflammatory responses, witnessed in chronic and persistent conditions. The current anti-oxidative stress and anti-inflammatory drugs are associated with detrimental effects, high dependence, high costs, inaccessibility, among other drawbacks; therefore, a need for alternatives is imperative. Despite the remarkable potential of medicinal plants, there are scanty empirical studies on their pharmacologic efficacy. The Phytexponent is an alcoholic polyherbal preparation of Allium sativum, Triticum repens, Echinacea purpurea, Viola tricolor and Matricaria chamomilla. In complementary medicine, the Phytexponent is used to boost immunity, to treat inflammatory disorders, oxidative stress, blood pressure, diabetes, stress/depression, among other conditions. However, there is no sufficient scientific data to support these healing claims. Therefore, in the current study evaluated the in vitro anti-inflammatory, antioxidant activities and qualitative phytochemical composition of the Phytexponent. The in vitro anti-inflammatory activities were evaluated using the inhibition of protein denaturation and the human erythrocyte (HRBC) membrane stabilization techniques. Antioxidant activities were evaluated by the 1,1-diphenyl-picryl-1-hydrazyl (DPPH) radical scavenging-, the hydroxyl radical scavenging- and catalase activities. Qualitative phytochemical screening was performed using standard procedures. The results showed a significantly higher percentage inhibition of heat-induced- and hypotonicity induced HRBC hemolysis by the Phytexponent at concentrations of 50 % and 100 %, compared with the percentage inhibitions of etanercept (p<0.05). No significant differences in percentage inhibitions of protein denaturation were observed among concentrations of 12.5 %,25.0 %,50.0 %,100.0 % of the Phytexponent and etanercept (25 mg/ml) (p˃0.05). Furthermore, the Phytexponent demonstrated high antioxidant activities against the DPPH- (IC50=0.00733%) and the hydroxyl- (IC50 = 0.716 %) radicals in vitro.The Phytexponent recorded significantly higher catalase activities at concentrations of 1 % and 0.1 % than those recorded by ascorbic acid at similar concentrations. Qualitative phytochemical screening revealed the presence of phenols, flavonoids, tannins, among other antioxidant associated phytochemicals. The bioactivities of the Phytexponent reported herein, were attributed to the presence of these phytochemicals. Further studies to establish specific mode(s) through which the Phytexponent exerts in vitro anti-inflammatory and antioxidant effects are encouraged. Moreover, in vivo anti-inflammatory and antioxidant activities should be done to determine the replicability of these findings in vivo. Bioassay-guided isolation of compounds responsible for the reported bioactivities herein should be done.

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF MILLINGTONIA HORTENSIS (L)

2017 ◽  
pp. 162-167
Author(s):  
Janaki A. ◽  
Kaleena P. K. ◽  
Elumalai D. ◽  
Hemalatha P. ◽  
Babu M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Leaf Extracts ◽  
Micro Organisms ◽  
Dpph Radical Scavenging

Objective: Millingtonia hortensis Linn (Bignoniaceae) is commonly known as cork tree and Akash neem. Aim of studies to determine the antioxidant activity and antibacterial activity.Methods: The antioxidant activity of different solvent extracts were measured by chemical analyses involving the assay of 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and super oxide radical scavenging activity.Results: Phytochemicals (secondary metabolites) screening of methanol, chloroform, ethanol, petroleum ether, aqueous leaf extracts revealed the presence of carbohydrates, tannins, saponins, flavonoids, alkaloids, betacyanins, phenols and coumarins.Conclusion: The presence of these phytochemicals and antioxidant capacity support the use of this plant as an antibacterial agent against the group of micro organisms tested. 

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