scholarly journals Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiao Wang ◽  
Shu Fu ◽  
Chen Zhang ◽  
Xin Nie ◽  
Wan Liao ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Periodic Acid ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Gastric Injury ◽  
Dependent Manner ◽  
Gastroprotective Effect ◽  
Ethanol Extracts ◽  
Magnolia Officinalis

Background. Magnolia officinalis Rehd. and Wils. is widely used in Asian countries because of its multiple pharmacological effects. This study investigated the gastroprotective effect and mechanisms of the ethanol extracts from the bark of Magnolia officinalis (MOE) against ethanol-induced gastric mucosal damage in rats. Methods. MOE was prepared by reflux extraction with 70% ethanol, and its main compounds were analyzed by UPLC-Q-Exactive Orbitrap-MS. DPPH, ABTS, and FRAP methods were used to evaluate the antioxidant capacity of MOE in vitro. The gastroprotective effects of MOE were evaluated by the area of gastric injury, H&E (hematoxylin-eosin), and PAS (periodic acid-Schiff). The mechanism was explored by measuring the levels of cytokines and protein in the NF-κB signaling pathway. Results. 30 compounds were identified from MOE, mainly including lignans and alkaloids. MOE presented a high antioxidant activity in several oxidant in vitro systems. Gastric ulcer index and histological examination showed that MOE reduced ethanol-induced gastric mucosal injury in a dose-dependent manner. MOE pretreatment significantly restored the depleted activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) enzymes, reduced malondialdehyde (MDA), and prostaglandin E2 (PGE2) levels in the gastric tissue in rats. In addition, MOE also inhibited the activation of nuclear factor kappa B (NF-κB) pathway and decreased the production of proinflammatory cytokines. Conclusions. The gastroprotective effect of MOE was attributed to the inhibition of oxidative stress and the NF-κB inflammatory pathway. The results provided substantial evidence that MOE could be a promising phytomedicine for gastric ulcer prevention.

Gastric mucosal protection with chronic mild restraint: role of endogenous prostaglandins

1984 ◽  
Vol 247 (2) ◽  
pp. G127-G132 ◽  
Author(s):  
J. L. Wallace ◽  
M. M. Cohen
Keyword(s):  
Chronic Stress ◽  
Mucosal Injury ◽  
Mucosal Damage ◽  
Gastric Fundus ◽  
Gastric Mucosal Damage ◽  
Gastric Injury ◽  
Protective Effects ◽  
Endogenous Prostaglandins

The role of endogenous prostaglandins (PGs) in adaptation of the rat gastric mucosa to chronic stress was examined. After 10 days of chronic mild restraint (CMR), gastric mucosal damage induced by orally administered 40% ethanol was significantly (P less than 0.01) less extensive than that to control mucosae. When the mucosal injury was produced by oral administration of acetylsalicylic acid (250 mg/kg), there was no protection afforded by prior exposure to CMR. Pretreatment with indomethacin (1 mg/kg ip) abolished the protective effects of CMR against ethanol injury. The indomethacin blockade of CMR protection was reversed by the subsequent administration of PGE2 (75 micrograms/kg po). Fundic samples from 10-day CMR rats synthesized three times as much PGE2 (P less than 0.01) and twice as much 6-keto-PGF1alpha (P less than 0.05) as control samples. Thromboxane B2 synthesis by control and CMR samples was not significantly different. The capacity of gastric fundus and antrum to synthesize PGE2 in vitro was higher in samples from CMR rats than from controls. These results suggest that the resistance to gastric injury that develops during chronic stress is mediated by endogenous prostaglandins.

A new in vitro model for ethanol-induced gastric mucosal damage

1999 ◽  
Vol 41 (4) ◽  
pp. 167-172 ◽  
Author(s):  
Yassir M.Al-Mulla Hummadi ◽  
Rafid A Najim ◽  
Imad B Farjou
Keyword(s):  
In Vitro Model ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Vitro Model

Pancreatic spasmolytic polypeptide protects the gastric mucosa but does not inhibit acid secretion or motility

1997 ◽  
Vol 273 (1) ◽  
pp. G112-G117 ◽  
Author(s):  
C. McKenzie ◽  
T. Marchbank ◽  
R. J. Playford ◽  
W. Otto ◽  
L. Thim ◽  
...  
Keyword(s):  
Acid Secretion ◽  
Mucosal Damage ◽  
Inhibitory Effect ◽  
Gastric Mucosal Damage ◽  
Conscious Rat ◽  
Immature Rat ◽  
Trefoil Peptide ◽  
Spasmolytic Polypeptide ◽  
Evoked Contractions

The objectives of these studies were to examine whether the trefoil peptide porcine pancreatic spasmolytic polypeptide (PSP) had gastric mucosal protectant properties similar to its human equivalent human spasmolytic polypeptide (hSP) and to confirm the antisecretory and antimotility action of the peptide. PSP and recombinant hSP reduced gastric mucosal damage caused by a combination of subcutaneous indomethacin and restraint stress in the conscious rat. At a dose of 500 micrograms/kg bolus plus 500 micrograms.kg-1.h-1 sc, PSP significantly reduced the total area of damage by 58%. PSP at a dose of 150 micrograms/kg iv had no inhibitory effect on pentagastrin-stimulated gastric acid secretion in the perfused stomachs of anesthetized rats. This lack of antisecretory activity was confirmed in vitro using an isolated stomach preparation from the immature rat. PSP and hSP at concentrations up to 800 nM did not inhibit electrically or chemically evoked contractions of the guinea pig ileum and duodenum in vitro. Thus antisecretory and antimotility actions do not underlie the mucosal protectant properties of PSP. PSP did, however, stimulate cell migration, and this may, at least in part, account for its protectant properties.

Stimulation of secretion into human and feline airways by Pseudomonas aeruginosa proteases

1991 ◽  
Vol 70 (5) ◽  
pp. 2259-2267 ◽  
Author(s):  
M. Somerville ◽  
P. S. Richardson ◽  
A. Rutman ◽  
R. Wilson ◽  
P. J. Cole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Alkaline Protease ◽  
Periodic Acid ◽  
Surface Epithelium ◽  
Dependent Manner ◽  
Periodic Acid Schiff ◽  
Concentration Dependent Manner ◽  
Human Bronchus ◽  
Secretion Inhibition

We have investigated the effect of elastase and alkaline protease from Pseudomonas aeruginosa on airway secretion into the trachea of anesthetized cats and from human bronchial mucosa in vitro. Secretory macromolecules were radiolabeled biosynthetically with two precursors in the cat, [3H]glucose and [35S]sulfate, and with [35S]-sulfate only in human tissue. Both enzymes (2.6 x 10(-9) to 1.3 x 10(-6)M elastase and 8 x 10(-9) to 2.4 x 10(-6)M alkaline protease) released radiolabeled macromolecules in a concentration-dependent manner from the two preparations. Purified elastase, 1.3 x 10(-6)M, released radiolabeled macromolecules (delta 3H = +397 +/- 72%, delta 35S 225 +/- 40% over control, P less than 0.001) and periodic acid-Schiff- (PAS) reactive glycoconjugates (delta PAS = +4.1 +/- 0.96 micrograms/min or +102 +/- 20%; P less than 0.01) from cat trachea, as did alkaline protease, 2.4 x 10(-6)M (delta 3H = +356 +/- 57%, delta 35S = +176 +/- 25%, delta PAS = +7.5 +/- 1.3 micrograms/min or 194 +/- 36%, P less than 0.001). Increases in 3H exceeded those of 35S, suggesting surface epithelium as the main source of secretion. Inhibition of enzyme activity abolished secretory effects. Both enzymes also stimulated secretion from human bronchus (e.g., with elastase, 1.3 x 10(-6)M: delta 35S = +331 +/- 67%, delta PAS = +4.3 +/- 0.92 micrograms/min or +131 +/- 24%, P less than 0.001; with alkaline protease, 2.4 x 10(-6)M: delta 35S = +220 +/- 67%, delta PAS = +12.7 +/- 3.2 micrograms/min or +575 +/- 245%, P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Anti-Nosemosis Activity of Aster scaber and Artemisia dubia Aqueous Extracts

2018 ◽  
Vol 62 (1) ◽  
pp. 27-38
Author(s):  
Jae Kwon Lee ◽  
Jeong Hwa Kim ◽  
Mina Jo ◽  
Balamurugan Rangachari ◽  
Jin Kyu Park
Keyword(s):  
Optimal Dose ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Active Components ◽  
Aqueous Fraction ◽  
Ethanol Extracts ◽  
Artemisia Dubia ◽  
Dose Dependent

Abstract In our previous study, we demonstrated that the ethanol extracts of Artemisia dubia (A. dubia) and Aster scaber (A. scaber) have anti-nosemosis activity. In our present study, we intend to establish the anti-nosemosis activity of aqueous, ethyl acetate (EA), and butanol (BuOH) extracts of A. dubia and A. scaber. In order to determine the optimal dose, we performed both in vitro and in vivo toxicity for all the extracts and also carried out anti-nosemosis experiments. Although all of the extracts (aqueous, EA, and BuOH) showed in vitro and in vivo anti-nosemosis activity in a dose-dependent manner, the aqueous extracts of A. dubia and A. scaber showed more potent anti-nosemosis activity than the EA and BuOH extracts. Moreover, an aqueous extract of A. dubia + A. scaber demonstrated stronger anti-nosemosis activity compared with the aqueous extracts of either A. dubia or A. scaber alone. Although the main ingredients in A. dubia and A. scaber remain unclear, our results suggest that the active components of A. dubia and A. scaber could dissolve in the aqueous fraction.

scholarly journals The Potential of Sweet Starfruit as a Gastroprotector of Gaster Damage Due to Free Radicals

2020 ◽  
Vol 2 (1) ◽  
pp. 1-6
Author(s):  
Alandra Rizhaqi Vastra ◽  
Mira Yustika Susilo ◽  
Neema Putri Prameswari ◽  
Bagus Pratama
Keyword(s):  
Gastric Ulcer ◽  
Free Radicals ◽  
Mucosal Damage ◽  
Potential Effect ◽  
Antioxidant Effect ◽  
Gastric Mucosal Damage ◽  
Flavonoid Content ◽  
Literature Study ◽  
Averrhoa Carambola ◽  
Active Substances

Gastric ulcer is a condition when deep gastric mucosa is damaged. This condition can be caused by oxidative stress which produces free radicals. Ulcer can be potentially prevented or treated with active substances contained by plants such as sweet starfruit. Sweet starfruit (Averrhoa carambola Linn) is a plant that has a high antioxidant effect with flavonoid content which plays a role in the process of gastric mucosal damage Objective to describe the potential gastroprotector effect of sweet starfruit (Averrhoa carambola Linn) in gastric damage caused by free radicals. This paper used literature study involving 18 libraries both national and international books and journal. Sweet starfruit (Averrhoa carambola Linn) contains flavonoid, this compound can work as an antioxidant by giving electrons to free radicals which causes the structure of free radicals to be more stable so that it can prevent the process of lipid peroxidase. Conclusions: Sweet starfruit has anti-ulcer potential effect with its antioxidant content which is flavonoids. Keywords: gastric ulcer, free radical, sweet starfruit, antioxidant

scholarly journals Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders

2021 ◽  
Vol 23 (1) ◽  
pp. 416
Author(s):  
Jingjing Liu ◽  
Wenyang Zhao ◽  
Chun Li ◽  
Tongyu Wu ◽  
Liang Han ◽  
...  
Keyword(s):  
Gastrointestinal Disease ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Health Concern ◽  
Disease Treatment ◽  
Atp Production ◽  
Anti Inflammation ◽  
Clinical Drug

Gastrointestinal disease is the most common health concern that occurs due to environmental, infectious, immunological, psychological, and genetic stress. Among them, the most frequent diseases are gastric ulcer (GU) and ulcerative colitis (UC). DSS-induced UC and ethanol-stimulated GU models resemble the pathophysiology of human gastrointestinal disease. The current study was designed to explore the anti-oxidation, anti-inflammation, anti-cell death properties of terazosin, an α-adrenergic receptor antagonist, in vivo and in vitro. Our results indicate that terazosin dramatically activates Pgk1, and upregulates glycose metabolism, evidenced by the enhanced ATP production and higher LDH enzymatic activity. Also, terazosin significantly enhances p-AKT expression and inhibits NF-κB p65 activation through abrogating the phosphorylation of IKBα, as well as lowers Caspase-1 and GSDMD expression. The findings in this study demonstrate that terazosin exhibits anti-inflammatory effects by downregulating NF-κB-GSDMD signal pathway, along with enhancing glycolysis for gastrointestinal disease treatment. Meanwhile, we also find terazosin ameliorates ethanol-induced gastric mucosal damage in mice. Collectively, as a clinical drug, terazosin should be translated into therapeutics for gastrointestinal disease soon.

scholarly journals GASTROPROTECTIVE EFFECT OF ONION PEEL (Allium Cepa L. Var Ascalonicum) EXTRACT ON WISTAR RATS INDUCED BY MEFENAMIC ACID

2020 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Awalya Rahma Putri ◽  
Dina Helianti ◽  
Nindya Shinta Rumastika
Keyword(s):  
Body Weight ◽  
Mefenamic Acid ◽  
Wistar Rats ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Weight Group ◽  
Onion Peel ◽  
Group 4 ◽  
Gastroprotective Effect ◽  
Group 1

Non Steroidal Anti-inflammatory Drugs (NSAID) have been implicated in the etiology of gastritis. Mefenamic acid is a non-selective NSAID used to treat mild to moderate pain and inflammation. Mefenamic acid induces gastritis through its inhibition of prostaglandin that results in increased HCl secretion, decreased mucin, and bicarbonate ion production. This study aimed to investigate the gastroprotective effect of onion peel extract (OPE) against mefenamic acid-induced gastritis. In this study, the gastroprotective effect of OPE was examined through mucosal integrity scoring. A total of 28 male Wistar rats were divided into 4 groups, normal (Group 1), mefenamic acid+NaCMC 0,5% (Group 2), mefenamic acid+OPE 600mg/kg body weight (Group 3), and mefenamic acid+OPE 1200mg/kg body weight (Group 4). All rats were sacrificed on day 15 then all gasters were collected. Histopathological examination was done under a microscope with 100X magnification. Administration of mefenamic acid without OPE at all significantly increased gastric mucosal damage (p<0,05). Administration of OPE 1200mg/kg body weight significantly decreased gastric mucosal damage (p<0,05). Group 4 shows no significant difference (p=0,66) with the normal group (Group 1). This indicates that administration of OPE 1200mg/kg body weight per oral ameliorates mefenamic acid-induced gastritis.

scholarly journals The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damage in Sprague-Dawley Rats

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0157431 ◽  
Author(s):  
Nahla Saeed AL-Wajeeh ◽  
Mohammed Farouq Halabi ◽  
Maryam Hajrezaie ◽  
Summaya M. Dhiyaaldeen ◽  
Daleya Abdulaziz Bardi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Sprague Dawley ◽  
Gastroprotective Effect ◽  
Sprague Dawley Rats
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