scholarly journals Chemical Composition and Antibacterial Activities of Eight Plant Essential Oils from Morocco against Escherichia coli Strains Isolated from Different Turkey Organs

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hassna Jaber ◽  
Asmaa Oubihi ◽  
Imane Ouryemchi ◽  
Rachid Boulamtat ◽  
Ali Oubayoucef ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectroscopy ◽  
Plant Essential Oils ◽  
E Coli ◽  
Gas Chromatography Mass Spectroscopy

The aim of the present study was to determine the chemical composition of eight plant essential oils and evaluate their antibacterial activity against Escherichia coli strains isolated from different turkey organs. The essential oils were extracted by hydrodistillation and analyzed using gas chromatography-mass spectroscopy. All essential oil yielded high in a range between 2.2 and 3.12%. Gas chromatography-mass spectroscopy (GC-MS) revealed that the major constituents of Thymus vulgaris, Ocimum basilicum, Artemisia herba-alba, and Syzygium aromaticum oils were thymol (41.39%), linalool (37.16%), camphor (63.69%), and eugenol (80.83%), respectively. Results of the E. coli sensitivity evaluated by the standard antimicrobial sensitivity method varied depending on the organ of isolation. Similarly, the essential oils antimicrobial activity determined by the disc diffusion method varied all along within the organs of isolation. T. vulgaris essential oil showed the highest effective antibacterial activity against E. coli isolated from the throat with an inhibition zone diameter value of up to 23.33 mm. However, all the essential oils showed antibacterial activity and the MIC and MBC values were in the range of 1/3000 to 1/100 (v/v) and the ratios MBC/MIC were equal to 1. In conclusion, this study showed that the essential oils could be promising alternatives to overcome E. coli multiresistance in turkey.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium)

2020 ◽  
Vol 10 (3) ◽  
pp. 272-278
Author(s):  
Ardalan Pasdaran ◽  
Satyajit D. Sarker ◽  
Lutfun Nahar ◽  
Azadeh Hamedi
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Main Compound ◽  
Aerial Parts ◽  
Anti Oxidant ◽  
Gas Chromatography Mass Spectroscopy

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.

scholarly journals Constituents of Peel and Leaf Essential Oils of Citrus Medica L

2009 ◽  
Vol 1 (2) ◽  
pp. 387-392 ◽  
Author(s):  
M. N. I. Bhuiyan ◽  
J. Begum ◽  
P. K. Sardar ◽  
M. S. Rahman
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectroscopy ◽  
Chemical Constituents ◽  
Citrus Medica ◽  
Leaf Oil ◽  
Gas Chromatography Mass Spectroscopy ◽  
Total Oil ◽  
Peel Oil

The chemical constituents of leaf and peel essential oil of Citrus medica L. were analysed by gas chromatography mass spectroscopy (GC-MS). Nineteen components accounting for 99.9% of the oil were identified in leaf oil. The major constituents are erucylamide (28.43%), limonene (18.36%) and citral (12.95%). The peel oil contains forty three components accounting for 99.8% of the total oil and the major components are isolimonene (39.37%), citral (23.12%) and limonene (21.78%). Keywords: Citrus medica; Essential oils; GC-MS; Erucylamide; Isolimonene. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1760   

scholarly journals Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

2022 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Mohammadreza Pajohi Alamoti ◽  
Behnaz Bazargani-Gilani ◽  
Razzagh Mahmoudi ◽  
Anna Reale ◽  
Babak Pakbin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Zataria Multiflora ◽  
Cuminum Cyminum ◽  
Plant Essential Oils ◽  
E Coli ◽  
Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

scholarly journals Analysis of white pepper essential oil components using gas chromatography-mass spectroscopy

2021 ◽  
pp. 230-234
Author(s):  
Purwaniati ◽  
Gilang Eka Permana ◽  
Indro Pamudjo
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectroscopy ◽  
White Pepper ◽  
Oil Components ◽  
Gas Chromatography Mass Spectroscopy

Introduction: White pepper is an important commodity used to produce essential oils. Differences in the oil components are determined by which region the peppers used were sourced from. Objectives: The aim of this study was to analyse the components of the essential oils produced by white peppers from different regions and to examine the specificity of these peppers. Methods: This analysis was carried out using the chromatography gas – mass spectroscopy (GC-MS) method on essential oil samples made from peppers obtained from the Java, Sumatera, Kalimantan, and Sulawesi Islands. Results: The results showed that white pepper essential oil contains 22 components, and that α-pinene, ꞵ–pinene, ∆-carene, sabinene, dl-limonene, and caryophyllene were major compounds within the oils. Furthermore, it showed that α-pinene, sabinene, and caryophyllene compounds were most found in samples from the Kalimantan, Sumatra, and Sulawesi Islands respectively. Meanwhile, those from Java Island contained ꞵ – pinene, ∆-carene, and caryophyllene in similar quantities.

scholarly journals Chemical Composition and Trypanocidal Activity of the Essential Oils from Hedychium coronarium J. Koenig (Zingiberaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Danilo Fernando Rodrigues ◽  
Angela María Arenas Velásquez ◽  
Carlos Cavaleiro ◽  
Lígia Salgueiro ◽  
Gilmárcio Zimmermann Martins ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Trypanosoma Brucei ◽  
Mass Spectroscopy ◽  
Colorimetric Method ◽  
Caryophyllene Oxide ◽  
Trypanocidal Activity ◽  
Hedychium Coronarium ◽  
Gas Chromatography Mass Spectroscopy

The composition of the essential oils (EO) from leaves and rhizomes of Hedychium coronarium was analyzed both by gas chromatography and gas chromatography-mass spectroscopy. Thirty and thirty-nine compounds were identified, respectively, in the oils from leaves and rhizomes, representing 88% and 86.1% of the whole compositions. Caryophyllene oxide is the major component in rhizomes while 1,8-cineole predominates in leaves oil. Essential oils and major components were tested for trypanocidal activity using procyclic forms of Trypanosoma brucei (427 and 29-13 strains). The cytotoxicity index (CI50), using the MTT colorimetric method, showed that essential oils and 1,8-cineole were inactive (>100 μg·mL−1. Nevertheless, caryophyllene oxide revealed a remarkable activity against both T. brucei strains (CI50 = 65.77 μg·mL−1 and 24.53 μg·mL−1, resp.), and the synergism between caryophyllene oxide plus pentamidine (1 : 1, v/v) highly increased the trypanocidal activity (<1.0 μg·mL−1).

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA MINYAK ATSIRI DARI TUMBUHAN SEMBUKAN (Paederia foetida L.) DENGAN METODE KROMATOGRAFI GAS-SPEKTROSKOPI MASSA (GC-MS)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Dewa Gde Agung Yuda Pratama ◽  
I Gusti Agung Gede Bawa ◽  
I Wayan Gede Gunawan
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectroscopy ◽  
Dibutyl Phthalate ◽  
Steam Distillation ◽  
Wild Plant ◽  
Diffusion Method ◽  
Paederia Foetida

Sembukan is a wild plant that is often attached to the house fenced and has a lot of chemicals compound, including essential oil. This study aims to determine the active compounds as antibacterial of sembukan essential oil. One point four mililitres of essential oil was obtained by steam distillation. The essential oils were then tested for antibacterial activity against Escherichia coli and Stapylococcus aureus using the well diffusion method in gel. Identification of the essential oils conducted using Chromatography Gas – Mass Spectroscopy (GC-MS). The results of this study indicated that the essential oils did not have specific antibacterial activity against the bacteria tested. Compounds contained in the essential oils of sembukan were hydrogenated Linalool, Eugenol, Tetradecane, Heksadecane, and Dibutyl phthalate.

scholarly journals Antibacterial and Biofilm Inhibitory Activity of Medicinal Plant Essential Oils Against Escherichia coli Isolated from UTI Patients

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1161 ◽  
Author(s):  
Rihab Lagha ◽  
Fethi Ben Abdallah ◽  
Badriah AL-Sarhan ◽  
Yassin Al-Sodany
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Inhibitory Action ◽  
Gas Chromatography Mass Spectrometry ◽  
Antibiofilm Activity ◽  
Plant Essential Oils ◽  
E Coli ◽  
Tract Infections

Urinary tract infections (UTIs), caused by Escherichia coli 80% to 85% of the time, are one of the most important causes of morbidity and health care spending affecting persons of all ages. These infections lead to many difficult problems, especially increasing resistance to antibiotic drugs. Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. In this study, we have investigated the antibacterial activity of five medicinal plant essential oils against UTIs caused by E. coli using disc diffusion and minimal inhibition concentration (MIC) methods. In addition, biofilm inhibitory action of oils was realized by crystal violet. Gas chromatography–mass spectrometry (GC–MS) analysis showed a variability between oils in terms of compound numbers as well as their percentages. Antibacterial activity was observed only in cases of Origanum majorana, Thymus zygis and Rosmarinus officinalis, while Juniperus communis and Zingiber officinale did not showed any effect towards E. coli isolates. T. zygis essential oil demonstrated the highest antibacterial activity against E. coli isolates, followed by O. majorana and R. officinalis. Further, oils showed high biofilm inhibitory action with a percentage of inhibition that ranged from 14.94% to 94.75%. R. officinalis oil had the highest antibiofilm activity followed by T. zygis and O. majorana. Accordingly, tested oils showed very effective antibacterial and antibiofilm activities against E. coli UTIs and can be considered as good alternative for antibiotics substitution.

scholarly journals Antibacterial activity chemical composition relationship of the essential oils from cultivated plants from Serbia

2011 ◽  
Vol 65 (5) ◽  
pp. 583-589 ◽  
Author(s):  
Nemanja Stankovic ◽  
Ljiljana Comic ◽  
Branislava Kocic ◽  
Dejan Nikolic ◽  
Tatjana Mihajilov-Krstev ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Cultivated Plants ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Broth Microdilution Method

The antibacterial effects of essential oils from Serbian cultivated plants, Thymus vulgaris L. (Lamiace) and Lavandula angustifolia L. (Lamiace) on different bacteria were investigated, with an emphasis on an antibacterial activity-chemical composition relationship. Essential oil was obtained from airdried aerial parts of the plants by hydrodistillation for 3 h using a Clevenger-type apparatus. The essential oil analyses were performed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) systems. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalyl acetate (38.23%) and linalool (35.01%) were main compounds in lavender oil. The antibacterial activity of the essential oils samples was tested towards 5 different bacteria: laboratory control strain obtained from the American Type Culture Collection and clinical isolates from different pathogenic media. Gram negative bacteria were represented by Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 43895 and Salmonella enteretidis ATCC 9027 while researched Gram positive strains were Bacillus cereus ATCC 8739 and Staphylococcus aureus ATCC 25923. A broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Essential oils from thyme have been found to have antimicrobial activity against all microorganisms tested, with a range of MIC values from 0.025 to 0.10 l/ml and MBC values from 0.05 to 0.78 l/ml. Lavender oils demonstrated MIC values from 0.025 to 0.20 l/ml and MBC values from 0.05 and 0.78 l/ml. Reference antibiotic tetracycline was active in concentrations between 0.025 and 0.05 l/ml. The Gram-positive bacteria were more sensitive to the essential oil of thyme, while Gram-negative bacteria were more sensitive to the essential oil of lavender. Essential oils from thyme and lavender may be used at low concentrations for prevention and treatment of infective diseases in animals and humans caused by pathogenic bacterial species.

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