scholarly journals Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jitendra Pandey ◽  
Sushma Bhusal ◽  
Laxman Nepali ◽  
Maya Khatri ◽  
Rasmita Ramdam ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Maximum Activity ◽  
Formalin Injection ◽  
Artemisia Vulgaris ◽  
Paw Edema ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Different Altitudes

This study aimed to evaluate and compare the in vivo chronic anti-inflammatory efficacy, from the ethyl acetate and ethanolic extracts of Artemisia vulgaris leaves, grown at three different altitudes in Nepal, by formalin-induced paw edema in Swiss albino mice. Edema was induced on the mice paw by administering 0.2% of formalin injection. Indomethacin was used as a standard drug at the concentration of 5 mg/kg of body weight. Ethyl acetate and ethanolic leaves extract, at the concentration of 200 mg/kg and 400 mg/kg, were used as test drugs. Standard drug and all the extracts were administered 30 min before formalin injection. The paw thickness was measured at 0, 1, 2, 3, 24, 48, and 72 hours after formalin injection, using a Vernier caliper. It was observed that both ethyl acetate and ethanolic extract from all the altitudes exhibited significant inhibition of paw edema ( p < 0.05 ) induced by formalin. Maximum activity was shown by 400 mg/kg of the plant leaf extract taken from the temperate zone, with 54.05% of paw edema inhibition, and it is almost similar to the inhibition of standard drug (56.75%). Moreover, the ethanolic extract was found to be more effective than ethyl acetate extract in all the plant samples. The results suggested that the anti-inflammatory effect of A. vulgaris leaves increases with an increase in altitudes and this plant can be used as a useful source of medicine to treat chronic inflammation.

scholarly journals Anti-Inflammatory Activity of Chrozophora rottleri Extracts on Carrageenan-Induced Rat Paw Edema

2016 ◽  
Vol 3 ◽  
pp. 20-26
Author(s):  
Mallikarjuna Rao Talluri ◽  
Battu Ganga Rao ◽  
Y. Venkateswaea Rao
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Bioactive Molecules ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

The present study was intended to evaluate Anti-inflammatory activity ofC. rottleriextracts (Hydroalcoholic, Methanol, Ethyl acetate and Hexane). The Anti-inflammatory activity ofC.rottleriextracts at doses of 125mg/kg, 250mg/kg and 500mg/kg using carrageenan induced rat paw edema model compared with standard drug (Indomethacin). The selected plant extracts significantly inhibited paw edema along with the standard drug Indomethacin. Of all extracts, methanol extract produced significant effect on reduction of increased paw thickness, hydro alcoholic and ethyl acetate extracts produced moderate percentage inhibition and hexane extract produced low level of percentage inhibition in reducing paw edema on carrageenan induced rats. In all extracts, methanol extract at a dose of 500mg/kg showed more percentage inhibition i.e . 53.47±2.19. From the results obtained during the study it is concluded thatC. rottlerihaving the bioactive molecule responsible for Anti-inflammatory activity by individually or by combination of different bio-active compounds present in it. Further is necessary for isolation and characterization of bioactive molecules which are responsible for the selected plant biological activities.

scholarly journals Anti-Inflammatory And Anti-Oxidant Activities Of Glochidion Daltonii Branch Extract

2018 ◽  
Vol 3 (2) ◽  
pp. 39
Author(s):  
Jintana Junlatat ◽  
Bungorn Sripanidkulchai
Keyword(s):  
Intraperitoneal Administration ◽  
Ethanolic Extract ◽  
Paw Edema ◽  
Sprague Dawley ◽  
Tropical Regions ◽  
Rat Paw Edema ◽  
Anti Oxidant ◽  
Anti Inflammatory

Background: Glochidion daltonii (MÜll. Arg.) Kurz, Euphorbiaceae family, is a native medicinal plant in tropical regions of Asia. In Thailand, it has traditionally been used for treating pain and mouth inflammation. Objective: This study aims to investigate the anti-inflammatory and anti-oxidant effects of G. daltonii ethanolic extract (GDE) both in vitro and in vivo. Method: The anti-inflammatory mechanism was examined in E.coli LPS-stimulated RAW264.7 cells by using semi-quantitative-reverse transcription-polymerase chain reaction and the potential anti-inflammatory effect of GDE was evaluated in Sprague-Dawley rat using carrageenan-induced rat paw edema method. In addition, DPPH assay was used for anti-oxidant evaluation. Result: The results showed that GDE at 0.063 - 0.250 mg/mL concentrations inhibited the expressions of tumor necrosis factor-α and interleukin-1β. Moreover, an intraperitoneal administration of GDE significantly reduced paw edema in rats. Furthermore, GDE showed high anti-oxidant property with IC50 values at 6.35 ± 0.28 µg/mL. Conclusion: The results support the traditionally use of GDE for treating inflammation.

scholarly journals Leaves of Stereospermum suaveolens DC Exhibit Anti-inflammatory and Anti-arthritic Potential Action in Experimental Animals

2021 ◽  
pp. 164-175
Author(s):  
R. R. Chanshetti ◽  
D. D. Bandawane
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Research Work ◽  
Ethyl Acetate Fraction ◽  
Significant Inhibition ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Aim: The experimental investigation of current research work was to identify traditional rich claim of Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential action in animals. Study design: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves evaluated at 125mg/kg, 250mg/kg and 500mg/kg (p.o.) doses for anti-inflammatory and anti-arthritic activity. Methodology: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves was evaluated for phytochemical investigation for total flavonoid content using UV spectroscopy and TLC study. Carrageenan induced rat paw edema (Acute method) and Freund’s complete adjuvant (FCA) induced chronic arthritis in wistar rats were used as an animal models to claim Stereospermum suaveolens DC leaves for anti-inflammatory and anti-arthritic potential.  The rat paw volume and percentage inhibition of the paw edema were evaluated for anti-inflammatory activity. The assessments of arthritis in rats were measured by haematological values and radiological examinations. Result: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves showed presence of total flavonoids and saponins. The significant inhibition in paw volume and edema (p < .01) obtained at 250mg/kg and 500mg/kg oral dose. These obtained results were established confirmation outcome for presence of rich flavonoid contents in Stereospermum suaveolens DC leaves and provides valuable source of bioactive phytocomponents.  Conclusion: Ethyl acetate fraction of Stereospermum suaveolens DC (Bignoniaceae) methanolic extract of leaves  showed significant inhibition of inflammatory reaction as compared to standard drug indomethacin Sterospermum Suaveolens DC leaves were showed potential therapeutic role in treatment of inflammation and arthritis cases.

scholarly journals Potential Nutraceutical Benefits of In Vivo Grown Saffron (Crocus sativus L.) As Analgesic, Anti-inflammatory, Anticoagulant, and Antidepressant in Mice

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1414
Author(s):  
Asif Khan ◽  
Nur Airina Muhamad ◽  
Hammad Ismail ◽  
Abdul Nasir ◽  
Atif Ali Khan Khalil ◽  
...  
Keyword(s):  
Capillary Tube ◽  
Forced Swim Test ◽  
Ethanolic Extract ◽  
Crocus Sativus ◽  
Herbaceous Plant ◽  
Standard Drug ◽  
Immobility Time ◽  
Anti Inflammatory ◽  
Edema Inhibition

Crocus sativus, a medicinally important herbaceous plant, has been traditionally used to cure coughs, colds, insomnia, cramps, asthma, and pain. Moreover, the therapeutic applications of saffron include its immunomodulatory and anticancer properties. The current experimental analysis was performed to explore the potential nutraceutical efficacy of corm, leaf, petal, and stigma of saffron ethanolic extracts as analgesic, anti-inflammatory, anticoagulant, and antidepressant using hot plate, carrageenan-induced paw edema, capillary tube and forced swim test, respectively in mice. The results indicated that among all the extracts, stigma ethanolic extract (SEE) represented maximum latency activity (72.85%) and edema inhibition (77.33%) followed by petal ethanolic extract (PEE) with latency activity and edema inhibition of 64.06 and 70.50%, respectively. Corm ethanolic extract (CEE) and leaf ethanolic extract (LEE) displayed mild analgesic activity of 22.40% and 29.07%, respectively. Additionally, LEE (53.29%) and CEE (47.47%) exhibited mild to moderate response against inflammation. The coagulation time of SEE (101.66 s) was almost equivalent to the standard drug, aspirin (101.66 s), suggesting a strong anticoagulant effect followed by PEE (86.5 s). LEE (66.83 s) represented moderate inhibitory effect on coagulation activity while CEE (42.83 s) showed neutral effect. Additionally, PEE and SEE also expressed itself as potential antidepressants with immobility time ≤76.66 s, while CEE (96.50 s) and LEE (106.83 s) indicated moderate to mild antidepressant efficacy. Based on the in vivo activities, saffron extract, particularly SEE and PEE, can be used as a potential nutraceutical and therapeutic agent due to its significant pharmacological activities.

scholarly journals Evaluation of in vivo and in vitro Biological Activities of Different Extracts of Cuscuta arvensis

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ufuk Koca ◽  
Esra Küpeli-Akkol ◽  
Nazim Sekeroglu
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Paw Edema ◽  
In Vivo Models ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Polar Extracts

In the present study, the potential effects of extracts from the whole plant of Cuscuta arvensis were studied in mice using the carrageenan-induced hind paw edema model for anti-inflammatory activity and the p-benzoquinone-induced writhing reflex for the assessment of antinociceptive activity. In order to obtain the extracts, the whole plant of C. arvensis was extracted with different solvents such as n-hexane, dichloromethane, ethyl acetate, methanol and distilled water. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The methanolic and water extracts inhibited the carrageenan-induced paw edema and p-benzoquinone-induced writhing reflex, whereas the other extracts showed only mild inhibitory antinociceptive and anti-inflammatory activities in these in vivo models. Additionally, the methanol and ethyl acetate extracts had higher scavenging ability then the non polar extracts.

Evaluation of Analgesic and Anti-inflammatory Effects of 5-amino-3-phenyl-1,3,5-thiadiazine-2-thione in Mice

2021 ◽  
Author(s):  
Junaid Athar ◽  
Zahid Rasul Niazi ◽  
Hamid Rasul Niazi ◽  
Hafiz Muhammad Irfan ◽  
Taseer Ahmad ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Inhibitory Effect ◽  
Latency Time ◽  
Paw Edema ◽  
Test Compound ◽  
Synthetic Compound ◽  
Standard Drug ◽  
Writhing Test ◽  
Anti Inflammatory

Abstract Purpose: The purpose of the study is to evaluate the analgesic and anti-inflammatory activity of new synthetic compound: 5-amino-3-phenyl-1,3,5-thiadiazine-2-thione and to compare its effects with the available standard drug, diclofenac sodium in mice. Methods: This test compound was evaluated for its anti-nociceptive activity using hot plate and writhing models, while for antiinflammatory activity evaluation, carrageenan and histamine-induced paw edema models were used. Results: In hotplate test, the latency time (in seconds), at 90 minutes of the administered doses (60 and 120 mg/kg) of 5-amino-3- phenyl -1.3.5-thiadiazine-2-thione were 19.5 seconds (p < 0.05) (Mean latency time) and 21.5 seconds (p < 0.01) (Mean latency time) respectively. In writhing test maximum inhibitory effect was demonstrated at a dose of 120 mg/kg with an average of 1.4 (p < 0.001) wriths (this inhibition was more significant) followed by 60 mg/kg (Mean number of wriths = 1.6) (p < 0.001) . The anti-inflammatory effect of the compound for 60 and 120 mg/kg doses was found to be significant and was more significant for 120 mg/kg in both histamine and carrageenan-induced paw edema models. Conclusion: It has been concluded that 5-amino-3-phenyl-1,3,5-thiadiazine-2-thione represents, dose-dependent antinociceptive and anti-inflammatory properties, which are statistically significant. The test compound showed a significant in-vivo peripheral analgesic effect in the writhing test, in comparison to the diclofenac sodium.

scholarly journals In-vitro and in-vivo anti-inflammatory activity of Plumeria indica Linn flowers

2020 ◽  
Vol 10 (5) ◽  
pp. 168-174
Author(s):  
Narendraa Yadav ◽  
Sourabh Jain ◽  
Karunakar Shukla
Keyword(s):  
Side Effects ◽  
Dose Level ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Test Protocol ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation is a reaction of a living vascularised tissue to an injury. Conventional or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it sometimes have serious side effects. So, number of herbal medicines is recommended for the treatment of inflammation that has no side effects. Hence our study focused to investigate the physicochemical, qualitative phytochemical analysis of bioactive compounds and In-vitro and In-vivo anti-inflammatory activity of Plumeria indica Linn (P. Indica) flowers extract which has boundless medicinal properties. The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The aqueous and ethanolic extract of P. Indica flowers was screened for in-vivo anti-inflammatory activity by carrageenan induced paw edema in rat model and in-vitro anti-inflammatory activity by human red blood cell membrane stabilization method. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids ect. Ethanolic extract showed best in vitro anti-inflammatory activity was screened for in vivo anti-inflammatory activity at the dose level of 250 and 500mg/kg. Indomethacin at the dose level of 10 mg/kg was used as reference standard drug. Both the extracts showed a dose dependent anti-inflammatory potential which provide scientific basis for the traditional claims of P. Indica flowers as an anti-inflammatory drug. Keywords: Plumeria indica Linn, Anti-inflammatory activity, Carrageenan, Human red blood cells membrane

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF MANILKARA ZAPOTA LEAF EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 130 ◽  
Author(s):  
Kamalakararao Konuku ◽  
Krishna Chaithanya Karri ◽  
Velliyur Kanniappan Gopalakrishnan ◽  
Zenebe Hagos ◽  
Haftom Kebede ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inflammatory Activity ◽  
In Vivo Studies ◽  
Paw Edema ◽  
Leaf Extracts ◽  
Manilkara Zapota ◽  
Anti Inflammatory

Objective: Manilkara zapota is a medicinal plant which is native to Mexico and Central America, and widely distributed in India. Various parts of this plant are traditionally used for treatment of several diseases, including inflammation-associated ailments. The main aim of the present study is to evaluate the anti-inflammatory potential of ethyl acetate and methanolic extracts of M. zapota leaf.Methods: In vitro secretary phospholipase A2 (PLA2) and 5-Lipoxygenase (5-LOX) assays and In vivo studies using carrageenan induced rat paw edema model were performed to assess the anti-inflammatory activity of M. zapota leaf extracts.Results: In vitro studies suggest that M. zapota leaf extracts exhibited significant SPLA2 and 5-LOX inhibitory activities. In in vivo studies M. zapota leaf extracts showed dose dependent inhibition of carrageenan induced paw edema in rats. The anti-inflammatory activity of ethyl acetate leaf extract was superior to methanolic extract.Conclusion: This study concluded that ethyl acetate leaf extract of M. zapotaexhibited significant anti-inflammatory activity and warranted further investigation to isolate and identify the components. 

Anti-Inflammatory and Antinociceptive Activity of Coumarins from Seseli gummiferum subsp. corymbosum (Apiaceae)

2009 ◽  
Vol 64 (1-2) ◽  
pp. 56-62 ◽  
Author(s):  
Alev Tosun ◽  
Esra Küpeli Akkol ◽  
Erdem Yeşilada
Keyword(s):  
Ethyl Acetate ◽  
Coumarin Derivatives ◽  
Paw Edema ◽  
Aerial Parts ◽  
Edema Model ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema ◽  
Ethyl Acetate Extracts

1n-Hexane and ethyl acetate extracts as well as coumarin derivatives obtained from the n-hexane extract of the aerial parts of Seseli gummiferum Pall. ex Sm. subsp. corymbosum (Boiss. & Heldr.) P.H. Davis (Apiaceae) were evaluated in vivo for their anti-inflammatory and antinociceptive activities. The n-hexane and ethyl acetate extracts of the species were shown to possess significant inhibitory activity against the carrageenan-induced hind paw edema and p-benzoquinone-induced writhing models in mice. Among the isolated coumarin derivatives; (−)-(3’S,4’S)-3’-acetoxy-4’-isovaleryloxy-3’,4’-dihydroseselin (), (−)-(3’S,4’S)-3’- acetoxy-4’-angeloyloxy-3’,4’-dihydroseselin (2), (+)-(3’S,4’S)-3’-hydroxy-4’-angeloyloxy-3’,4’- dihydroseselin (D-laserpitin) (3), (−)-(3’S,4’S)-3’-angeloyloxy-4’-hydroxy-3’,4’-dihydroseselin (4), and osthole (5), only the 3’-acetoxy derivatives 1 and 2 were found to possess potent antinociceptive and anti-infl ammatory activities, per os, without inducing any apparent acute toxicity as well as gastric damage, while all other compounds and extracts were found to be ineffective in the TPA-induced mouse ear edema model assay.

Design, Synthesis, Characterization and in silico molecular docking studies and in vivo anti-inflammatory Activity of Pyrazoline clubbed Thiazolinone Derivatives

2020 ◽  
Vol 17 ◽  
Author(s):  
Deepak Kumar Singh ◽  
Mayank Kulshreshtha ◽  
Yogesh Kumar ◽  
Pooja A Chawla ◽  
Akash Ved ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Biological Activities ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Docking Score ◽  
Chemical Structures ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Cox 1

Background: The pyrazolines give the reactions of aliphatic derivatives, resembling unsaturated compounds in their behavior towards permanganate and nascent hydrogen. This nucleus has been associated with various biological activities including inflammatory. Thiazolinone is a heterocyclic compound that contains both sulfur and nitrogen atom with a carbonyl group in their structure.Thiazolinone and their derivatives have attracted continuing interest because of their various biological activities, such as anti-inflammatory, antimicrobial, anti-proliferative, antiviral, anticonvulsant etc. The aim of the research was to club pyrazoline nucleus with thiazolinone in order to have significantanti-inflammatory activity. The synthesized compounds were chemically characterized for the establishment of their chemical structures and to evaluate as anti-inflammatory agent. Method: In the present work, eight derivatives of substituted pyrazoline (PT1-PT8) were synthesized by a three step reaction.The compounds were subjected to spectral analysis by Infrared, Mass and Nuclear magnetic resonance spectroscopy and elemental analysis data. All the synthesized were evaluated for their in vivo anti-inflammatory activity. The synthesized derivatives were evaluated for their affinity towards target COX-1 and COX-2, using indomethacin as the reference compound molecular docking visualization through AutoDock Vina. Results: Compounds PT-1, PT-3, PT-4 and PT-8 exhibited significant anti-inflammatory activity at 3rd hour being 50.7%, 54.3%, 52.3% and 57% respectively closer to that of the standard drug indomethacin (61.9%).From selected anti-inflammatory targets, the synthesized derivatives exhibited better interaction with COX-1 and COX-2 receptor, where indomethacin showed docking score of -6.5 kJ/mol, compound PT-1 exhibited highest docking score of -9.1 kJ/mol for COX-1 and compound PT-8 having docking score of 9.4 kJ/mol for COX-2. Conclusion: It was concluded that synthesized derivatives have more interaction with COX-2 receptors in comparison to the COX-1 receptors because the docking score with COX-2 receptors were very good. It is concluded that the synthesized derivatives (PT-1 to PT-8) are potent COX-2 inhibitors.

Sign in / Sign up

Export Citation Format

Share Document