scholarly journals GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Amanda Mara Teles ◽  
João Victor Silva-Silva ◽  
Juan Matheus Pereira Fernandes ◽  
Ana Lucia Abreu-Silva ◽  
Kátia da Silva Calabrese ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Future Research ◽  
Total Phenolic ◽  
Syzygium Aromaticum ◽  
Antitrypanosomal Activity ◽  
Low Cytotoxicity ◽  
Therapeutic Alternatives

Syzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC50 of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC50 of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC50 of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; p  = 0.002) and eugenol (48.05%; p  = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis.

scholarly journals Phytochemical Analysis and Potential Biological Activities of Essential Oil from Rice Leaf

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 546 ◽  
Author(s):  
Truong Minh ◽  
Tran Xuan ◽  
Truong Van ◽  
Yusuf Andriana ◽  
Tran Viet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Atmospheric Pressure Chemical Ionization ◽  
Biological Activities ◽  
Methyl Ricinoleate ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Chemical Compositions ◽  
Rice Leaf ◽  
Rice Leaves

Although many investigations on phytochemicals in rice plant parts and root exudates have been conducted, information on the chemical profile of essential oil (EO) and potent biological activities has been limited. In this study, chemical compositions of rice leaf EO and in vitro biological activities were investigated. From 1.5 kg of fresh rice leaves, an amount of 20 mg EO was obtained by distillation and analyzed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI) to reveal the presence of twelve volatile constituents, of which methyl ricinoleate (27.86%) was the principal compound, followed by palmitic acid (17.34%), and linolenic acid (11.16%), while 2-pentadecanone was the least (2.13%). Two phytoalexin momilactones A and B were first time identified in EO using ultra-performance liquid chromatography coupled with electrospray mass spectrometry (UPLC/ESI-MS) (9.80 and 4.93 ng/g fresh weight, respectively), which accounted for 7.35% and 3.70% of the EO, respectively. The assays of DPPH (IC50 = 73.1 µg/mL), ABTS (IC50 = 198.3 µg/mL), FRAP (IC50 = 700.8 µg/mL) and β-carotene oxidation (LPI = 79%) revealed that EO possessed an excellent antioxidant activity. The xanthine oxidase assay indicated that the anti-hyperuricemia potential was in a moderate level (IC50 = 526 µg/mL) as compared with the standard allopurinol. The EO exerted potent inhibition on growth of Raphanus sativus, Lactuca sativa, and two noxious weeds Echinochloa crus-galli, and Bidens pilosa, but in contrast, the growth of rice seedlings was promoted. Among the examined plants, the growth of the E. crus-galli root was the most inhibited, proposing that constituents found in EO may have potential for the control of the problematic paddy weed E. crus-galli. It was found that the EO of rice leaves contained rich phytochemicals, which were potent in antioxidants and gout treatment, as well as weed management. Findings of this study highlighted the potential value of rice leaves, which may provide extra benefits for rice farmers.

scholarly journals Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Kamel Msaada ◽  
Nidhal Salem ◽  
Olfa Bachrouch ◽  
Slim Bousselmi ◽  
Sonia Tammar ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

scholarly journals In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

2018 ◽  
Vol 7 (9) ◽  
pp. 283 ◽  
Author(s):  
Sherif Hassan ◽  
Kateřina Berchová-Bímová ◽  
Miroslava Šudomová ◽  
Milan Malaník ◽  
Karel Šmejkal ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Clinical Practice ◽  
Essential Oil ◽  
Biological Activities ◽  
Structural Parameters ◽  
Antihypertensive Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Ic50 Values ◽  
Main Component

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 µg/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 µg/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 µg/mL compared with that of cisplatin (IC50 = 10.08 µg/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 µg/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 µg/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 µg/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

scholarly journals Chemical Composition, Enantiomeric Distribution, and Biological Activities of Rhododendron anthopogon Leaf Essential Oil from Nepal

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Noura S. Dosoky ◽  
Prabodh Satyal ◽  
Suraj Pokharel ◽  
William N. Setzer
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Cytotoxic Activities ◽  
Blood Disorders ◽  
Leaf Essential Oil ◽  
Microbroth Dilution

Rhododendron anthopogon D. Don., a small compact Himalayan shrub growing in Nepal, is a known medicinal plant used to treat sore throat, colds, blood disorders, bone disease, potato allergies, and vomiting, and to relieve liver disorders, headaches and back pain. The present study investigated the chemical composition and bioactivities of the leaf essential oil from R. anthopogon from Dhankuta, Nepal. The essential oil from leaves was obtained by hydrodistillation and a detailed chemical analysis was conducted by gas chromatography – mass spectrometry (GC-MS). The enantiomeric distribution of monoterpenoid components was determined using chiral gas chromatography and represents the first chiral examination of R. anthopogon essential oil. The essential oil was screened for antimicrobial activity using the microbroth dilution test, and for cytotoxic activity against MCF-7, MDA-MB-231, and 5637 using the MTT assay. A total of 70 volatile components were identified from the essential oil. The major components were α-pinene (21.5%), δ-cadinene (13.8%), β-pinene (9.5%), limonene (5.9%), δ-amorphene (4.6%), α-muurolene (4.5%), and ( E)-caryophyllene (3.2%) with other minor constituents (< 3%). The essential oil showed marginal antibacterial and cytotoxic activities, but no antifungal effects.

scholarly journals Chemical Composition and Biological Activities of Oregano Essential Oil and Its Fractions Obtained by Vacuum Distillation

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1904 ◽  
Author(s):  
Magdalena de J. Rostro-Alanis ◽  
Juan Báez-González ◽  
Cynthia Torres-Alvarez ◽  
Roberto Parra-Saldívar ◽  
José Rodriguez-Rodriguez ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Vacuum Distillation ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Oregano Essential Oil ◽  
Almost All

Oregano (Poliomintha longiflora) essential oil (Ooil) is a product of high commercial value and many applications, including chemotherapy. Aiming to achieve the best use of this resource, the present study focuses on the characterization of separated fractions of Ooil by fractional vacuum distillation at low pressure. Four fractions (F1–F4) and undistilled oil (Unoil) were separated from Ooil and analyzed for their chemical composition and biological activities, such as antioxidant and antimicrobial activities. Gas chromatography–mass spectrometry shows differences in the composition among the fractions and Ooil. The amount of monoterpenes oxygenated (MO), sesquiterpenes hydrocarbon (SeH) and monoterpenes hydrocarbon (MH) varied between the fractions in ranges of 1.51–68.08, 3.31–25.12 and 1.91–97.75%, respectively. The major concentrations of MO and SeH were observed in F4 and Unoil. On the other hand, the highest concentrations of MH were found in F1 and F2, while the lowest were in F4 and Unoil. These results were correlated with the biological activity. Free-radical scavenging activity varied among fractions, with F4 and Unoil showing the highest activity. The antimicrobial test showed that F4 and Unoil had the highest activity in almost all cases. The correlation between the variables studied in the different fractions allows the definition of the particular properties for each one of them.

scholarly journals Antileishmanial Potential of Propolis Essential Oil and Its Synergistic Combination With Amphotericin B

2020 ◽  
Vol 15 (1) ◽  
pp. 1934578X1989956 ◽  
Author(s):  
Ayari Jihene ◽  
Essid Rym ◽  
Karoui Jabri Ines ◽  
Hammami Majdi ◽  
Tabbene Olfa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Amphotericin B ◽  
Leishmania Major ◽  
Antileishmanial Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Low Cytotoxicity ◽  
Promising Source

The antileishmanial activity of Tunisian propolis essential oil (EO) and its combination with amphotericin B was investigated against 2 local clinical strains of Leishmania: Leishmania major and Leishmania infantum. The cytotoxic potential of this EO was evaluated against macrophage Raw264.7. Combination of propolis EO and amphotericin B was investigated using the checkerboard method. The propolis sample was collected from the region of Beni Khalled, a Tunisian city located west of Cape Bon (Nabeul). Its location is particular since it is near to sea with a steppe climate and the predominance of citrus trees. The EO was obtained by Clevenger-type apparatus. Its chemical composition was identified using gas chromatography with flame ionization detector and gas chromatography-mass spectrometry analysis. Our results demonstrate that Tunisian propolis EO exhibit good antileishmanial activity against L. major and L. infantum promastigotes (IC50 = 5.29 ± 0.31 and 3.67 ± 0.52 µg/mL, respectively) and amastigotes (IC50 = 7.38 ± 0.45 and 4.96 ± 0.24 µg/mL, respectively). Moreover, it reduced significantly the parasite proliferation on a dose-dependent response (95%) with low cytotoxicity (selectivity index = 16.18 and 23.33, respectively). Its combination with amphotericin B showed a synergistic potential (fractional inhibitory concentration = 0.37). Interestingly, the data suggest that propolis EO was involved in macrophage activation by hyperproduction of NO. A total of 51 compounds were identified in the propolis EO. The major compound identified was α-pinene (36.7% ± 2.36%) followed by α-cedrol (6.7% ± 0.10%), totarol (6.6% ± 0.09%), and dehydroabietane (5.2% ± 0.10%). Our findings suggest that Tunisian propolis might constitute a promising source for antileishmanial molecules.

scholarly journals GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil

2019 ◽  
Vol 44 (3) ◽  
pp. 388-396
Author(s):  
Ashfaq Ahmad Khan ◽  
Muhammad Shoaib Amjad ◽  
Saboon
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Colorimetric Method ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Dpph Assay ◽  
Mentha Arvensis ◽  
Ms Analysis

Abstract Background Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities. Materials and methods Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method. Results The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result. Conclusion As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings.

scholarly journals Development of a Millet Starch Edible Film Containing Clove Essential Oil

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 184 ◽  
Author(s):  
Alaa G. Al-Hashimi ◽  
Altemimi B. Ammar ◽  
Lakshmanan G. ◽  
Francesco Cacciola ◽  
Naoufal Lakhssassi
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Control Sample ◽  
Barrier Properties ◽  
Edible Film ◽  
Gas Chromatography Mass Spectrometry ◽  
Syzygium Aromaticum ◽  
Preservation Method ◽  
Clove Essential Oil

Medicinal plants contain various secondary metabolites. The present study analyzed the essential oil of buds from clove (Syzygium aromaticum L.; Family: Myrtaceae) using gas chromatography-mass spectrometry (GC-MS). GC-MS analysis showed the presence of six major phytoconstituents, such as eugenol (66.01%), caryophyllene (19.88%), caryophyllene oxide (5.80%), phenol, 2-methoxy-4-(2-propenyl)-acetate (4.55%), and humulene (3.75%). The effect of clove essential oils (CEO) at 0%, 1%, 2%, and 3% (w/w) on the mechanical and barrier properties of starch films was evaluated. The tensile strength (TS) and elongation (E) of films with clove essential oil were 6.25 ± 0.03 MPa and 5.67% ± 0.08%, respectively. The antioxidant activity of the films significantly increased the millet starch film and presented the lowest antioxidant activity (0.3%) at a 30 minute incubation for the control sample, while increasing CEO fraction in the starch film lead to an increase in antioxidant activity, and the 3% CEO combined film presented the highest antioxidant activity (15.96%) at 90 min incubation. This finding could be explained by the incorporation of clove oil containing antioxidant properties that significantly increased with the incorporation of CEO (p < 0.05). A zone of inhibition ranging from 16 to 27 mm in diameter was obtained when using a concentration of CEO ranging from 1% to 3%. We also observed the presence of an antimicrobial activity on several tested microorganism including Escherichia coli, Pseudomonas aeruginosa, Enterobacter sp, Bacillus cereus, Staphylococcus aureus, and Trichoderma fungi. Thus, the current study reveals the possibility of using a millet starch edible film as a preservation method.

scholarly journals Clove Essential Oil (Syzygium aromaticum L. Myrtaceae): Extraction, Chemical Composition, Food Applications and Essential Bioactivities for Human Health

2021 ◽  
Author(s):  
José Nabor Haro-González ◽  
Gustavo Adolfo Castillo-Herrera ◽  
Moisés Martínez-Velázquez ◽  
Hugo Espinosa-Andrews
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Human Health ◽  
Biological Activities ◽  
Syzygium Aromaticum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Food Applications ◽  
Clove Essential Oil ◽  
Interest In Research

Clove (Syzygium aromaticum L. Myrtaceae) is an aromatic plant widely cultivated in tropical and subtropical countries, rich in volatile compounds and antioxidants such as eugenol, &beta;-caryophyllene, and &alpha;-humulene. Clove essential oil has received considerable interest due to its wide application in the perfumery, cosmetic, health, medical, flavoring, and food industries. Clove essential oil has relevant biological activities to human health, including antimicrobial, antioxidant, and insecticide. This review describes the effect of the extraction method (hydrodistillation, steam distillation, ultrasound-assisted extraction, microwave-assisted extraction, cold pressing, and supercritical fluid extraction) on the chemical composition of essential oil and its correlation with their biological activities. Likewise, are summarized the main compounds and their reported biological activities. Furthermore, the main applications in clove essential oil in the food industry are presented. Finally, this review presents the new biological activities such as anti-inflammatory, analgesic, anesthetic, antinociceptive and anticancer, which are beneficial for human health. This review aims to compile the effect of different methods of extracting clove essential oil on chemical composition, food applications, as well as a current description of biological activities of interest to human health. Biological activities have increased interest in research into this essential oil and its future applications in the food or pharmaceutical industry.

scholarly journals Origanum vulgare ssp. hirtum (Lamiaceae) Essential Oil Prevents Behavioral and Oxidative Stress Changes in the Scopolamine Zebrafish Model

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7085
Author(s):  
Luminita Capatina ◽  
Edoardo Marco Napoli ◽  
Giuseppe Ruberto ◽  
Lucian Hritcu
Keyword(s):  
Oxidative Stress ◽  
Cognitive Impairment ◽  
Essential Oil ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Protective Effects ◽  
Origanum Vulgare ◽  
Zebrafish Model ◽  
Stress Changes ◽  
Brain Oxidative Stress

Origanum vulgare ssp. hirtum has been used as medicinal herbs promoting antioxidant, anti-inflammatory, antimicrobial, and neuroprotective activities. We investigated the protective effects and the mechanism of O. vulgare ssp. hirtum essential oil (OEO) on cognitive impairment and brain oxidative stress in a scopolamine (Sco)-induced zebrafish (Danio rerio) model of cognitive impairment. Our results show that exposure to Sco (100 µM) leads to anxiety, spatial memory, and response to novelty dysfunctions, whereas the administration of OEO (25, 150, and 300 µL/L, once daily for 13 days) reduced anxiety-like behavior and improved cognitive ability, which was confirmed by behavioral tests, such as the novel tank-diving test (NTT), Y-maze test, and novel object recognition test (NOR) in zebrafish. Additionally, Sco-induced brain oxidative stress and increasing of acetylcholinesterase (AChE) activity were attenuated by the administration of OEO. The gas chromatography–mass spectrometry (GC-MS) analyses were used to elucidate the OEO composition, comprising thymol (38.82%), p-cymene (20.28%), and γ-terpinene (19.58%) as the main identified components. These findings suggest the ability of OEO to revert the Sco-induced cognitive deficits by restoring the cholinergic system activity and brain antioxidant status. Thus, OEO could be used as perspective sources of bioactive compounds, displaying valuable biological activities, with potential pharmaceutical applications.

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