scholarly journals Ovine Progressive Pneumonia: Diagnosis and Seroprevalence in the South of Sonora, Mexico

2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Mercedes Yannin Borquez Cuevas ◽  
Juan Francisco Hernández Chávez ◽  
Betsy Armenta Leyva ◽  
Jesús Raymundo Cedillo Cobián ◽  
Ramón Miguel Molina Barrios
Keyword(s):  
Serological Test ◽  
Positive Outcome ◽  
Pcr Analysis ◽  
Histopathological Study ◽  
Follicular Hyperplasia ◽  
Molecular Tests ◽  
Visna Virus ◽  
First Case ◽  
Ovine Progressive Pneumonia ◽  
Pneumonia Diagnosis

Ovine progressive pneumonia (OPP) is the most severe presentation of small ruminant lentivirus (SRLV) infection known as Maedi-Visna. Serological evidence in Mexico of the presence of this lentivirus was published in 1986. After that, studies revealed that SRLVs have a broad distribution in Mexico by detecting antibodies or/and molecular tests; however, a descriptive case of the disease has not been published. This work’s objective was to describe the diagnosis of a case of OPP through lesion description, serology, and molecular test. The histopathological study showed that lymph follicular hyperplasia, interstitial pneumonia, and smooth muscle hyperplasia were presented. The serological test demonstrated specific antibodies against the Maedi-Visna virus, and PCR analysis demonstrated a positive outcome. These results include the criteria for the diagnosis of OPP. The serological prevalence of this disease is presented, contributing to the knowledge of the ecology of this disease in the world. This work is the first case report of ovine progressive pneumonia in Mexico and evidence of seroprevalence in sheep herds from Sonora, Mexico.

scholarly journals ALK-positive Histiocytosis of Umbilicus Subcutaneous with KIF5B-ALK Fusion: a Case Report

2021 ◽  
Author(s):  
Yili Zhu ◽  
Jun Fan ◽  
Bo Huang ◽  
Ying Wu ◽  
Heshui Shi ◽  
...  
Keyword(s):  
Pathological Examination ◽  
Older Children ◽  
Anaplastic Lymphoma ◽  
Molecular Tests ◽  
Spindle Cells ◽  
First Case ◽  
Alk Positive ◽  
Painless Mass ◽  
Lymphocytic Infiltrates

Abstract Background: Since the discovery of the first case of Anaplastic lymphoma kinase (ALK) -positive histiocytosis in 2008, originally described as a systemic, self-limiting disease in infants, the range of ALK-positive histiocytosis has recently been expanded to include localized diseases in older children and young adults. Case presentation: We present the case of an 18-year-old female with periumbilical painless mass for 5 months, who underwent a resection of the mass. Pathological examination showed the tumor consists predominantly of fascicular to storiform growth of nonatypical spindle cells, admixed with lymphocytic infiltrates. The tumor spindle cells were diffusely positive for CD68, CD163 and ALK. Further, molecular tests revealed ALK gene fusion: Kinesin Family Member 5B (KIF5B) (E24)-ALK (E20), confirmed ALK-positive histiocytosis. The tumor has not recurred one and a half years after resection by follow-up examination.Conclusion: ALK-positive histiocytosis in local lesion can achieve remission by complete resection and clinical follow-up showed a favorable prognosis.

scholarly journals B-cell acute lymphoblastic leukemia as a secondary malignancy following diffuse large B-cell lymphoma

2019 ◽  
Vol 11 (2) ◽  
Author(s):  
Daria Gaut ◽  
Anthony Bejjani ◽  
Joshua Sasine ◽  
Gary Schiller
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
B Cell ◽  
Cell Lymphoma ◽  
Lymphoblastic Leukemia ◽  
B Cell Lymphoma ◽  
Pcr Analysis ◽  
Secondary Malignancy ◽  
Large B Cell Lymphoma ◽  
First Case ◽  
Large B Cell

Secondary acute lymphoblastic leukemia (ALL) is a rare disease that has not been well characterized compared with secondary myelodysplastic syndrome or secondary acute myeloid leukemia. We present a report of two patients who developed ALL following complete remission of diffuse large B-cell lymphoma (DLBCL). The first case is more consistent with a therapy-related ALL as a PCR analysis of bone marrow aspirate revealed a distinct clone and the mixed-lineage leukemia gene rearrangement, commonly associated with exposure to topoisomerase II inhibitors. The second case is more consistent with clonal evolution given positive MYC and BCL2 fusion signals in the original diagnosis of DLBCL and the secondary ALL.

scholarly journals Prurigo nodularis due to Mycobacterium tuberculosis

2009 ◽  
Vol 58 (12) ◽  
pp. 1649-1651 ◽  
Author(s):  
Laura Saporito ◽  
Ada Maria Florena ◽  
Claudia Colomba ◽  
Diego Pampinella ◽  
Paola Di Carlo
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Unknown Origin ◽  
Mycobacterial Infection ◽  
Skin Lesions ◽  
Pcr Analysis ◽  
Prurigo Nodularis ◽  
First Case ◽  
Chronic Skin ◽  
First Time ◽  
Time Culture

Prurigo nodularis (PN) is a rare chronic skin disorder of unknown origin. Here we describe what is believed to be the first case of PN associated with tuberculosis. For the first time, culture and PCR analysis of skin biopsy confirmed the presence of Mycobacterium tuberculosis complex in PN skin lesions. The pruritus and skin lesions resolved following antitubercular therapy. Our case provides further evidence in favour of a link between PN and mycobacterial infection.

scholarly journals Struma ovarii: two case reports of a rare teratoma of the ovary

2020 ◽  
Vol 2020 (12) ◽  
Author(s):  
Obed Rockson ◽  
Christine Kora ◽  
Abdelbassir Ramdani ◽  
Aabdi Basma ◽  
Tariq Bouhout ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Case Reports ◽  
Thyroid Tissue ◽  
Ovarian Teratoma ◽  
Histopathological Study ◽  
Histopathological Diagnosis ◽  
Struma Ovarii ◽  
Rare Type ◽  
Benign Condition ◽  
First Case

Abstract Struma ovarii is an extremely rare type of ovarian teratoma distinguished by the unusual presence of thyroid tissue. It is usually a benign condition; however, malignant transformation is sometimes detected. The diagnosis relies on histopathological examination and is infrequently made on routine investigations. We report two cases of struma ovarii: one benign and the other malignant. The first case involved a 27-year-old woman who underwent a right ovarian cystectomy for an incidental ultrasonographic finding of a solid right adnexal mass. The diagnosis of a benign struma was made after histopathological study. The second case involved a 68-year-old woman who underwent a right salpingo-oophorectomy for a right ovarian bulky mass and the histopathological diagnosis was consistent with that of a malignant struma. We examine the challenges involved in the diagnosis and management of this rare entity.

scholarly journals Molecular epidemiology and high mortality of Crimean-Congo hemorrhagic fever in Oman: a re-emerging infection

2018 ◽  
Author(s):  
Seif Al-Abri ◽  
Roger Hewson ◽  
Hanan Al-Kindi ◽  
Idris Al-Abaidani ◽  
Amina Al-Jardani ◽  
...  
Keyword(s):  
High Mortality ◽  
Animal Husbandry ◽  
Hemorrhagic Fever ◽  
Healthcare Associated Infection ◽  
Single Strain ◽  
Reading Frame ◽  
Crimean Congo Hemorrhagic Fever ◽  
Molecular Tests ◽  
First Case ◽  
Serious Disease

Background Crimean-Congo hemorrhagic fever (CCHF) is a serious disease with a high fatality rate reported in many countries. The first case of CCHF in Oman was detected in 1995 and serosurveys have suggested widespread infection of humans and livestock throughout the country. Methodology Cases of CCHF reported to the Ministry of Health (MoH) of Oman between 1995 and 2017 were retrospectively reviewed. Diagnosis was confirmed by serology and/or molecular tests in Oman. Stored RNA from recent cases was studied by sequencing the complete open reading frame (ORF) of the viral S segment at Public Health England, enabling phylogenetic comparisons to be made with other S segments of strains obtained from the region. Findings Of 88 cases of CCHF, 4 were sporadic in 1995 and 1996, then none occurred until 2011. From 2011-2017, incidence has steadily increased and 19 (23.8%) of 80 cases clustered around Eid Al Adha. The median (range) age was 33 (15-68) years and 79 (90%) were male. The major risk for infection was contact with animals and/or butchering in 73/88 (83%) and only one case was related to tick bites alone. Severe cases were over-represented: 64 (72.7%) had a platelet count < 50 x 109/L and 32 (36.4%) died. There was no intrafamilial spread or healthcare-associated infection. The viral S segments from 11 patients presenting in 2013 and 2014 were all grouped in Asia 1 (IV) lineage. Conclusions CCHF is well-established throughout Oman, with a single strain of virus present for at least 20 years. Most patients are men involved in animal husbandry and butchery. The high mortality suggests that there is substantial under-diagnosis of milder cases. Preventive measures have been introduced to reduce risks of transmission to animal handlers and butchers and to maintain safety in healthcare settings.

scholarly journals Vibrio cholerae non-O1 - the first reported case of keratitis in a healthy patient

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wei-Dar Chen ◽  
Li-Ju Lai ◽  
Wei-Hsiu Hsu ◽  
Tsung-Yu Huang
Keyword(s):  
Vibrio Cholerae ◽  
Corneal Ulcer ◽  
Positive Outcome ◽  
Ocular Injury ◽  
Significant Morbidity ◽  
Chain Reaction ◽  
Case Presentation ◽  
First Case ◽  
Polymerase Chain ◽  
The Right

Abstract Background Vibrio cholerae non-O1 is a virulent pathogen that causes significant morbidity and mortality in humans. Herein, we report a case of corneal ulcer caused by this pathogen. Case presentation A 59-year-old fisherman with no systemic history was struck in the right eye by a marine shrimp and developed keratitis. Corneal scrapping culture revealed the presence of the V. cholerae non-O1, and its identification was confirmed by Analytical Profile Index 20E system and polymerase chain reaction. He was successfully treated with topical levofloxacin (0.3%) and fortified amikacin (12.5 mg/mL) for 2 weeks. The visual acuity recovered to 20/25 after treatment without complications. Conclusions This is the first case report of keratitis caused by V. cholerae non-O1 strain. Ocular injury by marine creatures and contaminated seawater can contribute to severe corneal ulcer. Early diagnosis can be achieved by meticulous history taking and a comprehensive laboratory workup. Simultaneously, an effective antibiotic therapy can lead to a positive outcome.

Failure of experimental vaccines to protect against infection with ovine progressive pneumonia (maedi-visna) virus

1987 ◽  
Vol 13 (3) ◽  
pp. 201-204 ◽  
Author(s):  
Randall C. Cutlip ◽  
Howard D. Lehmkuhl ◽  
Kim A. Brogden ◽  
Mary Jo F. Schmerr
Keyword(s):  
Visna Virus ◽  
Maedi Visna Virus ◽  
Ovine Progressive Pneumonia

Role of MCM as an adjuvant to urine cytology in the diagnosis of bladder cancer.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e15011-e15011
Author(s):  
Nadira Narine ◽  
Bensita M.V Thottakam ◽  
Alessia Donnini ◽  
Sushant Dhanvijay ◽  
Kasra Saeb-Parsy ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Positive Outcome ◽  
Urine Cytology ◽  
Low Grade ◽  
High Grade ◽  
Minichromosome Maintenance ◽  
Molecular Tests ◽  
In Vitro Diagnostic ◽  
Mcm Proteins

e15011 Background: Urine cytology, a cheap non invasive first line test (PAP) has traditionally been used for the diagnosis of urothelial carcinoma (UC) as it has excellent sensitivity for the detection of high grade and in situ lesions. However, sensitivity for low grade carcinoma is rather varied as this is a problematic area with various mimics leading to both over and under diagnoses. To overcome this dilemma, a number of biomarkers and molecular tests have been employed with mixed results and varied financial implications. We report on a relatively cheap and easily adopted in vitro diagnostic test, Minichromosome maintenance (MCM) proteins, as an adjuvant to cytology in the diagnosis of both low and high grade bladder cancer (BC). MCM proteins play an important regulatory role in eukaryotic DNA replication and is expressed only as normal cells progress from G0 into G1/S phase of the cell cycle. However, over expression has been demonstrated in neoplasia in a range of sites including urothelium. Methods: 106 patients from gross haematuria (GH, 39) and cystoscopic surveillance (CS, 67) clinics were investigated for newly diagnosed and recurrent BC respectively by MCM and cytology with histological outcome being used as the gold standard. Results: Using biopsy positive outcome, MCM and cytology had a sensitivity of 91.7%, specificity of 86.6%, PPV of 66.7% and NPV of 97.3%. There was noticeably improved correlation of MCM and cytology with increasing grade of BC (Table). Conclusions: We showed the combination of MCM and cytology to be highly sensitive and specific for the determination of CIS, G2, and G3 BC with improved detection of G1 bladder cancer. The combination of MCM and cytology suggests that this offers promise as a novel diagnostic biomarker in GH and CS patients. [Table: see text]

The structure of seroprevalence to the SARS-CoV-2 virus among residents of the Moscow Region during the period of epidemic incidence of COVID-19

2020 ◽  
Vol 18 (4) ◽  
pp. 17-26
Author(s):  
A.Yu. Popova ◽  
◽  
E.B. Ezhlova ◽  
A.A. Melnikova ◽  
O.M. Mikailova ◽  
...  
Keyword(s):  
Large Scale ◽  
Medical Center ◽  
Age Groups ◽  
Maximum Level ◽  
Test System ◽  
Moscow Region ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Population Immunity ◽  
First Case

A pandemic of the disease caused by the SARS-CoV-2 virus (hereinafter referred to as COVID-19) was announced by WHO in February 2020. In the Moscow Region (MR), the first case of the disease was detected on March 7, 2020 in a woman who arrived from Switzerland. Four weeks later, a rapid rise began, the peak of which fell on the 20th week (May 11, 2020 – May 17, 2020), which was replaced by a gradual steady decline that lasted for 13 weeks. Objective. To determine the level and structure of population immunity to the SARS-CoV-2 virus among the population of the Moscow Region between June 22, 2020 and July 11, 2020. Patients and methods. A serological study of seroprevalence among residents of MR to COVID-19 was carried out from June 22, 2020 till July 11, 2020 during the period of a steady decline in the incidence. The work was conducted as part of the first stage of the large-scale Rospotrebnadzor program to assess population immunity to the COVID-19 virus among the population of the Russian Federation. Volunteers for the study were selected by the method of questioning and randomization. The analysis included the results of a survey of 2688 people. The number of volunteers in all age groups was equal. The content of specific IgG to the COVID-19 nucleocapsid was determined by the enzyme-linked immunosorbent assay using a test system manufactured by the FBIS SRCAMB according to the manufacturer's instructions. Results. The results of the study showed that the collective immunity of the total population of the Moscow Region was 21.0%. The maximum level of population immunity was established in children aged 14–17 years (30.7%) and 1–6 years old (25.2%). There were no statistically significant differences in the level of seroprevalence between men and women. As a result of seroepidemiological research, it was shown that in the medical center, in the presence of contacts with patients with COVID-19, the risk of infection increases by 2.5 times. After infection with COVID-19, antibodies were detected in 78.7% of cases. In persons with a positive result of the PCR analysis obtained earlier, antibodies were detected in 82.8% of cases. A high proportion of asymptomatic infection among seropositive volunteers was found, which amounted to 83.4%. Key words: coronaviruses, SARS-CoV-2, incidence, seroprevalence, the Moscow Region, population, COVID-19

The first case of fungal endogenous endophthalmitis caused by Aspergillus nidulans: Diagnostic and therapeutic challenge

2020 ◽  
pp. 112067212093208
Author(s):  
Lucia Mata-Moret ◽  
Clara Monferrer-Adsuara ◽  
Laura Hernández-Bel ◽  
Marisa Hernández-Garfella ◽  
Miriam Torrecillas-Muelas ◽  
...  
Keyword(s):  
Vision Loss ◽  
Visual Outcome ◽  
Endogenous Endophthalmitis ◽  
Pcr Analysis ◽  
Causative Organism ◽  
Systemic Corticosteroids ◽  
First Case ◽  
Dismal Prognosis ◽  
History Of ◽  
High Index Of Suspicion

Significance: Fungal endogenous endophthalmitis is an uncommon and potentially blinding infection. Aspergillus is a causative organism in immunocompromized although is virulent enough to afflict immunocompetents. Their propensity to affect macula usually results in a dismal prognosis; thus, improving visual outcome has always been challenging to clinicians. A. nidulans has only rarely been implicated in exogenous endophthalmitis. Purpose: To report the first case of A. nidulans endogenous endophthalmitis. Case report: An asthmatic 42-year-old female presented with sudden unilateral vision loss due to a submacular abscess that progressively worsened in a matter of days. Vitreous PCR analysis after an urgent vitrectomy was positive for A. nidulans with no active systemic foci found. Oral and intravitreal Voriconazole was prescribed but multiple reactivations led to three vitrectomies in total alongside with subretinal Voriconazole, abscess aspiration, and endolaser. There was complete resolution of the infection and, although visual acuity was poor due to macular scar, enucleation was avoided. Conclusion: Although uncommon, we must consider Aspergillus as the causative organism in apparently immunocompetent patients with history of recent systemic corticosteroids treatment, especially if they suffer a broncopulmonary disorder. Aspergillus is an aggressive organism so a high index of suspicion along with early diagnosis and prompt treatment is the key for better outcomes. We highlight A. nidulans as the causative agent as there are no other reported cases.

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