scholarly journals Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Takenori Suga ◽  
Michihiko Usui ◽  
Satoru Onizuka ◽  
Kotaro Sano ◽  
Tsuyoshi Sato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcriptome Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Negative Control Group ◽  
Negative Control ◽  
Histone Deacetylation ◽  
Control Group ◽  
Spheroid Culture

A spheroid is known as a three-dimensional culture model, which better simulates the physiological conditions of stem cells. This study is aimed at identifying genes specifically expressed in spheroid-cultured human periodontal ligament mesenchymal stem cells (hPDLMSCs) using RNA-seq analysis to evaluate their functions. Transcriptome analysis was performed using spheroid and monolayer cultures of hPDLMSCs from four patients. Cluster and Gene Ontology analyses revealed that genes involved in cell-cell adhesion as well as the G2/M and G1/S transitions of mitotic cell cycles were strongly expressed in the monolayer culture group. However, genes involved in the negative regulation of cell proliferation, histone deacetylation, and bone morphogenetic protein signaling were strongly expressed in the spheroid culture group. We focused on the transcription factor nuclear receptor subfamily 4 group A member 2 (NR4A2) among the genes that were strongly expressed in the spheroid culture group and analyzed its function. To confirm the results of the transcriptome analysis, we performed real-time polymerase chain reaction and western blotting analyses. Interestingly, we found that the mRNA and protein expressions of NR4A2 were strongly expressed in the spheroid-cultured hPDLMSCs. Under osteogenic differentiation conditions, we used siRNA to knock down NR4A2 in spheroid-cultured hPDLMSCs to verify its role in osteogenesis. We found that NR4A2 knockdown significantly increased the levels of mRNA expression for osteogenesis-related genes alkaline phosphatase (ALP), Osteopontin (OPN), and type 1 collagen (COL1) (Student’s paired t -test, p < 0.05 ). ALP activity was also significantly increased when compared to the negative control group (Student’s paired t -test, p < 0.05 ). Additionally, spheroid-cultured hPDLMSCs transfected with siNR4A2 were cultured for 12 days, resulting in the formation of significantly larger calcified nodules compared to the negative control group (Student’s paired t -test, p < 0.05 ). On the other hand, NR4A2 knockdown in hPDLMSC spheroid did not affect the levels of chondrogenesis and adipogenesis-related genes under chondrogenic and adipogenic conditions. These results suggest that NR4A2 negatively regulates osteogenesis in the spheroid culture of hPDLMSCs.

Impact of bone-marrow-derived mesenchymal stem cells on adriamycin-induced chronic nephropathy

2014 ◽  
Vol 92 (9) ◽  
pp. 733-743 ◽  
Author(s):  
Mohamed Sarhan ◽  
Hanaa El Serougy ◽  
Abdelaziz M. Hussein ◽  
Mohamed El-dosoky ◽  
Mohamed A. Sobh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Survival Rate ◽  
Caspase 3 ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Intravenous Injections ◽  
Tubulointerstitial Damage

Objectives: To study the effects of bone-marrow-derived mesenchymal stem cells (BM-MSCs) on adriamycin (ADR)-induced chronic nephropathy in rats. Methods: 60 male Sprague–Dawley rats were distributed among 3 groups (20 rats each): (i) the negative control group, which was normal rats that received saline (vehicle); (ii) the positive control (ADR) group, which was rats that received 2 intravenous injections of ADR into the penile vein at 14 day intervals without treatment, and (iii) the MSC group, which were rats treated as for the ADR group that were also given 2 intravenous injections of MSCs (5 days after each ADR injection). Results: ADR caused a significant reduction in animal body mass, survival rate, hemoglobin (Hb) content, serum albumin, and renal GSH, and significantly increased serum levels of triglycerides, cholesterol, urinary protein excretion and kidney injury molecule-1 (KIM-1), renal MDA, as well as caspase-3 expression and glomerular and tubulointerstitial damage compared with the negative control group. MSC treatment failed to improve animal survival rate, body mass, Hb level, proteinuria, or hypoalbuminemia; however, it mildly improved the serum BUN, hyperlipidemia, caspase-3 expression, urinary levels of KIM-1, renal oxidative stress markers, and glomerular and tubulointerstitial damage score. Conclusion: administration of BM-MSCs during induction of ADR nephropathy provides partial protection, which could be due to improvements in the levels of of endogenous antioxidants, reduction of apoptosis, and maintenance of the integrity of the glomerular membrane.

Gene Expression Regulation underlying Osteo-, Adipo-, and Chondro-Genic Lineage Commitment of Human Mesenchymal Stem Cells

2013 ◽  
pp. 76-94 ◽  
Author(s):  
Ana M. Sotoca ◽  
Michael Weber ◽  
Everardus J. J. van Zoelen
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Expression Regulation ◽  
Expression Profiles ◽  
Human Mesenchymal Stem Cells ◽  
Gene Expression Profiles ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Lineage Commitment

Human mesenchymal stem cells have a high potential in regenerative medicine. They can be isolated from a variety of adult tissues, including bone marrow, and can be differentiated into multiple cell types of the mesodermal lineage, including adipocytes, osteocytes, and chondrocytes. Stem cell differentiation is controlled by a process of interacting lineage-specific and multipotent genes. In this chapter, the authors use full genome microarrays to explore gene expression profiles in the process of Osteo-, Adipo-, and Chondro-Genic lineage commitment of human mesenchymal stem cells.

Gene expression profiles of early adipogenesis in human mesenchymal stem cells

Gene ◽  
2004 ◽  
Vol 340 (1) ◽  
pp. 141-150 ◽  
Author(s):  
Shih-Chieh Hung ◽  
Ching-Fang Chang ◽  
Hsiao-Li Ma ◽  
Tain-Hsiung Chen ◽  
Larry Low-Tone Ho
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Expression Profiles ◽  
Human Mesenchymal Stem Cells ◽  
Gene Expression Profiles

Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

2019 ◽  
Vol 120 (7) ◽  
pp. 11842-11852 ◽  
Author(s):  
Simone Ortiz Moura Fideles ◽  
Adriana Cassia Ortiz ◽  
Amanda Freire Assis ◽  
Max Jordan Duarte ◽  
Fabiola Singaretti Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteoblast Differentiation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Source

scholarly journals Gene Expression Profiles of Human Adipose Tissue-Derived Mesenchymal Stem Cells Are Modified by Cell Culture Density

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83363 ◽  
Author(s):  
Dae Seong Kim ◽  
Myoung Woo Lee ◽  
Keon Hee Yoo ◽  
Tae-Hee Lee ◽  
Hye Jin Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Adipose Tissue ◽  
Culture Density

scholarly journals Identification of immune-infiltrated hub genes as potential biomarkers of Moyamoya disease by bioinformatics analysis

2021 ◽  
Author(s):  
Fa Jin ◽  
Chuanzhi Duan
Keyword(s):  
Moyamoya Disease ◽  
Expression Profiles ◽  
Molecular Transport ◽  
Neutrophil Infiltration ◽  
Negative Control Group ◽  
Negative Control ◽  
Functional Enrichment ◽  
Control Group ◽  
Hub Genes ◽  
Immune Infiltration

Abstract Background Moyamoya disease (MMD) is a unique chronic progressive cerebrovascular disease. The molecular mechanism behind pathophysiology is still elusive. This study aims to determine the key genes and their roles in the immune infiltration of MMD.Methods We download raw gene expression profiles (GSE157628, GSE141024) of cerebrovascular tissue from GEO database. Identify differentially expressed genes (DEGs) and perform functional enrichment analysis. The CIBERSORT deconvolution algorithm was used to analyze the proportion of immune cell infiltration between MMD and negative control group. We screened for neutrophil-associated DEGs, constructed a protein-protein interaction network (PPI) using STRING, and clarified hub genes using the Cytoscape plugin MCODE analysis. The receiver operating characteristic (ROC) curve is applied to test and filter the best gene signature.Results A total of 570 DEGs were detected, including 212 downregulated and 358 up-regulated genes. Reactome and KEGG enrichment revealed that DEGs are involved in the cell cycle, molecular transport, and metabolic pathways. The immune infiltration profile demonstrates that MMD cerebrovascular tissues contained a higher proportion of neutrophils, monocytes, and NK cells than negative control group. PPI network and MCODE cluster identified 9 DEGs (UNC13D, AZU1, PYCARD, ELANE, SDCBP, CCL11, CCL15, CCL20, and CXCL5) associated with neutrophil infiltration. ROC results showed that UNC13D has good specificity and sensitivity (AUC = 0.7846).Conclusions The characteristics of immune infiltration in the cerebrovascular tissues of MMD patients and abnormal expression of hub genes provide new insights for understanding MMD progression. UNC13D is promising to be one of the candidate molecules to determine neutrophil infiltration characteristics in MMD.

Gene expression profiles of differentiated and undifferentiated adipose derived mesenchymal stem cells dynamically seeded onto a processed nerve allograft

Gene ◽  
2020 ◽  
Vol 724 ◽  
pp. 144151 ◽  
Author(s):  
Femke Mathot ◽  
Nadia Rbia ◽  
Roman Thaler ◽  
Allen T. Bishop ◽  
Andre J. Van Wijnen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles
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