scholarly journals Interleukin-18: A Novel Participant in the Occurrence, Development, and Drug Therapy of Obliterative Bronchiolitis Postlung Transplantation

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ping Shu ◽  
Wei Zhang ◽  
Yanfei Zhang ◽  
Yanfeng Zhao ◽  
Yuping Li ◽  
...  
Keyword(s):  
Drug Treatment ◽  
Negative Control ◽  
Obliterative Bronchiolitis ◽  
Mouse Strains ◽  
Control Group ◽  
Natural Immunity ◽  
Interleukin 18 ◽  
Blank Control Group ◽  
Tracheal Occlusion ◽  
Ifn Γ

Background. Obliterative bronchiolitis (OB) was a main cause of deterioration of long-term prognosis in lung transplant recipients after the first posttransplant year. Proinflammatory cytokine interleukin-18 (IL-18) strengthened both the natural immunity and acquired immunity and played an important role in organ transplantation. The roles of IL-18 in the occurrence, development, and drug treatment of OB remained unclear. Methods. Small interfering RNA (siRNA) against mouse IL-18 (siRNA-IL-18) was used to silence IL-18 expression. Mouse heterotopic tracheal transplantation model was used to simulate OB. Recipient mice were divided into 5 groups ( n = 12 ) according to donor mouse strains and drug treatment: isograft group, allograft group, allograft+tacrolimus group, allograft+azithromycin group, and allograft+tacrolimus+azithromycin group. The luminal obliteration rates were pathological evaluation. Expressions of cytokines and MMPs were detected by real-time PCR, western blot, and enzyme chain immunosorbent assay (ELISA). Results. The luminal obliteration rates of IL-18 of the siRNA-IL-18 group were significantly lower than those of the negative control group ( p < 0.0001 ) and the blank control group ( p = 0.0002 ). mRNA expressions of IFN-γ, EMMPRIN, MMP-8, and MMP-9 of the siRNA-IL-18 group were significantly lower than those of the negative and blank control groups. No tracheal occlusion occurred in grafts of the isograft group. The rates of tracheal occlusion of the allograft group, allograft+tacrolimus group, allograft+azithromycin group, and allograft+tacrolimus+azithromycin group were 72.17 ± 4.66 %, 40.33 ± 3.00 %, 38.50 ± 2.08 %, and 23.33 ± 3.24 %, respectively. There were significant differences between the 4 groups ( p < 0.001 ). Serum protein expressions of IL-17 ( p = 0.0017 ), IL-18 ( p = 0.0036 ), IFN-γ ( p = 0.0102 ), and MMP-9 ( p = 0.0194 ) were significantly decreased in the allograft+tacrolimus+azithromycin group compared to the allograft group. Conclusions. IL-18 could be a novel molecular involved in the occurrence, development, and drug treatment of OB.

scholarly journals The influence of whole sonicated Pseudomonas aeruginosa antigens on experimental p. aeruginosa arthritis in rabbits

2014 ◽  
Vol 38 (1) ◽  
pp. 1-10
Author(s):  
Ahmed Q. Al-Awadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inflammatory Reaction ◽  
Rabbit Model ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Post Inoculation ◽  
Bacterial Isolation ◽  
Internal Organs ◽  
Ifn Γ

To study the influence of whole sonicated Pseudomonas aeruginosa antigens (WSPAgs) on experimental arthritis induced by this bacteria, 15 rabbits were divided into 3 equal groups. The 1st group was inoculated intraarticular with 0.2 ml of p. aeruginosa suspension (2×108 cfu/ml), the 2nd group was immunized with WSP Ags, and inoculated intraarticular as in the 1st group. The 3rd group was served as negative control group. At 30 day post inoculation the immunized (2nd) group showed increase in the cellular (DTH and IFN-γ) and humeral (IgG) immunity and moderate bacterial isolation from joints, blood and internal organs comparing with other groups. The 1st group showed sever symptoms and inflammatory reaction as well as very obvious gross and microscopical lesions in their joints including supportive reaction, pyogranulomatous lesions, necrosis, pannus reaction and destruction of the articular cartilage and the lesion extended to the subchondral bone leading to osteomyelitis, the 2nd group (immunized group) expressed mild to moderate inflammatory reaction and the microscopic examination indicate that the lesion was confined in the articular capsule. In conclusion the whole Pseudononas aeruginosa sonicated Ags (WSPAgs) protect the joint from the experimental infection by P. aeruginosa in a rabbit model.

scholarly journals Effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 1019-1027
Author(s):  
Lijie He ◽  
Jing Wang ◽  
Dandan Chang ◽  
Dandan Lv ◽  
Haina Li ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Luciferase Reporter ◽  
Control Group ◽  
Rt Pcr ◽  
Blank Control Group ◽  
Cervical Cancer Cells ◽  
Mrna And Protein Expression

AbstractObjectiveThis article aims to investigate the effect of miRNA-200b on the proliferation and apoptosis of cervical cancer cells by targeting RhoA.MethodsHeLa cells of cervical cancer were divided into five groups: blank control group, negative control group (miRNA-200b mimic NC), miRNA-200b mimic group, RhoA-negative control group, and RhoA overexpression group. Cells were collected 48 h after transfection. The expression levels of miRNA-200b were detected by RT-PCR. Target relationship between miRNA-200b and RhoA was verified by the dual-luciferase reporter assay. RhoA mRNA and protein expression were detected by western blot and RT-PCR methods. Flow cytometry was used to detect the apoptosis of cells in each group, and the CCK8 method was used to detect the proliferation of cells in each group. The mRNA and protein expression of Bax and cyclin D1 were detected by RT-PCR and western blot.ResultsThe results of the dual luciferase reporter assay showed that RhoA was the target gene of microRNA 200b. Compared with the blank control group and the miRNA-200b mimic-NC group, the proportion of apoptotic cells increased significantly in the miRNA-200b mimic group, and the proliferation of cells was inhibited (P < 0.05). After overexpression of RhoA, the percentage of apoptotic cells decreased and the ability of cell proliferation increased significantly (P < 0.05).ConclusionmiRNA-200b can inhibit the proliferation and promote the apoptosis of cervical cancer cells by targeting the RhoA gene.

H19/miR-675-5p Targeting SFN Enhances the Invasion and Metastasis of Nasalpharyngeal Cancer Cells

2019 ◽  
Vol 12 (4) ◽  
pp. 324-333 ◽  
Author(s):  
Ting Zhang ◽  
Fanghong Lei ◽  
Tao Jiang ◽  
Lisha Xie ◽  
Pin Huang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cells ◽  
Carcinoma Cell ◽  
Negative Control ◽  
Control Group ◽  
Invasion And Metastasis ◽  
Blank Control Group ◽  
Nasopharyngeal Carcinoma Cell ◽  
Significant Difference ◽  
Metastatic Nasopharyngeal Carcinoma

<P>Aims: The aim is to study the role of miR-675-5p coded by long non-coding RNA H19 in the development of Nasopharyngeal Cancer (NPC) and whether miR-675-5p regulates the invasion and metastasis of NPC through targeting SFN (14-3-3&#963;). The study further validated the relationship between H19, miR-675-5p and SFN in NPC and their relationship with the invasion and metastasis of NPC. </P><P> Methods: Western blot was used to detect the expression of 14-3-3&#963; protein in immortalized normal nasopharyngeal epithelial cells NP69 and different metastatic potential NPC cells, 6-10B and 5-8F. At the same time, to find out the relationship between 14-3-3&#963; protein and the expression of H19 and miR-675-5p, the expression of H19 and miR-675-5p in normal nasopharynx epithelial cells NP69 and varied nasopharyngeal carcinoma cells 6-10B and 5-8F were quantified by real-time PCR. MiR-675-5p mimic and inhibitor were transfected into NPC 6-10B to over-express and down-express miR-675-5p; miR-675-5p mimic negative control and inhibitor negative control were transfected into NPC 6-10B as control groups. The effect of over-expression and down-expression by miR-675-5p on the expression of 14-3-3&#963; protein was detected by Western blotting. The 3’-UTR segments of SFN, containing miR-675-5p binding sites were amplified by PCR and the luciferase activity in the transfected cells was assayed to detect whether SFN is the direct target of miR-675-5p. Transwell and scratch assays were used to verify the changes in NPC invasion and metastasis ability of mimics and inhibitors transfected with miR-675-5p. </P><P> Results: The expression of 14-3-3&#963; protein in normal nasopharynx epithelial cells NP69 is significantly higher than in varied nasopharyngeal carcinoma cells, 6-10B and 5-8F (P<0.05), and the 14-3-3&#963; protein levels in low-metastatic nasopharyngeal carcinoma cell 6-10B is higher than in high-metastatic nasopharyngeal carcinoma cell 5-8F. The expression of H19 and miR-675-5p are significantly higher in NPC cells than in NP69 cell (P<0.05). The expression of H19 and miR-675-5p in high-Metastatic nasopharyngeal carcinoma cell 5-8F was higher than in low-Metastatic nasopharyngeal carcinoma cell 6-10B. The expression of 14-3-3&#963; protein in miR-675-5p mimic cells was significantly lower than in mimic NC (negative control) group and blank control group. However, compared with the blank control group, mimic NC showed no significant difference in 14-3-3&#963; protein between the two groups. The miR-675-5p inhibitor group was significantly higher than the inhibitor NC group and the blank control group (p<0.05), but there was no significant difference in the expression of 14-3-3&#963; protein in the inhibitor NC group and the blank control group (p>0.05). Dual-luciferase reporter assay system shows the 3’-UTR segments of SFN containing miR-675-5p binding sites. SFN was the target gene of miR-675-5p. </P><P> Conclusion: 14-3-3&#963; is downregulated in NPC and is involved in the development of NPC. H19 and miR- 675-5p are upregulated in NPC, which is related to the development of NPC. The over-expression of miR- 675-5p inhibits the expression of 14-3-3&#963; protein. SFN is the target gene of miR-675-5p. MiR-675-5p targets SFN, downregulates its protein expression and promotes the invasion and metastasis of NPC.</P>

Intra-Ocular Pressure and Morphological Changes of the Trabecular Meshwork Cells After Ultraviolet A/ Riboflavin Corneal Crosslinking

2019 ◽  
Vol 9 (12) ◽  
pp. 1712-1717
Author(s):  
Li Ren ◽  
Changxia Cui ◽  
Bing Wang ◽  
Zhiwei Li ◽  
Yuzhen Liu ◽  
...  
Keyword(s):  
Cell Density ◽  
Trabecular Meshwork ◽  
Statistical Significance ◽  
Negative Control ◽  
Control Group ◽  
Ultraviolet A ◽  
Blank Control Group ◽  
Corneal Crosslinking ◽  
Intra Ocular Pressure ◽  
Ocular Pressure

The current study aimed to investigate the intra-ocular pressure (IOP) and morphological variations in trabecular meshwork (TM) cells from rabbit eye followed being treated with ultraviolet A/riboflavin corneal crosslinking (CXL) onto the TM area, by which to further verify the possibility of applying TM CXL onto the treatment of glaucoma in clinics in the future. Rabbits were randomly classified into the 3 groups, such as blank control, negative control, and treated groups. Afterwards, we carried out the measurement of IOP with schiotz tonometer and morphological evaluation of the TM cells via light microscopy and transmission electron microscopy before and after CXL within 12 weeks. No statistical significance was found between blank control group and negative control group as for the interspace area of TM cells, cell density of TM cells or IOP in the current study. In comparison to blank control group, interspace area in TM cells declined on day (D) 1 after CXL, followed by a significant increase within the following follow-ups from week (W) 1 to W12, and reached a stable situation on W2. Compared to the blank control group, the cell density of TM cells declined on D1 after CXL, followed by a slight increase within the following follow-ups from W1 to W12, and reached a stable situation on W2. Meanwhile, compared with the blank control group, the IOP increased on D1 after CXL, followed by a slight decrease within the following follow-ups from W1 to W12, and reached a stable situation on W2. In conclusion, the present study revealed an important decrease on IOP and predominant enlargement in the interspace area of TM cells after CXL when in comparison to blank control group, which implies the possibility of applying CXL for the treatment of glaucoma in clinics.

scholarly journals Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap(d41) protein and analysis of its immunogenicity in mice and swine

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Ya-Yi Chen ◽  
Wei-Chen Yang ◽  
Yu-Kang Chang ◽  
Chi-Young Wang ◽  
Wei-Ru Huang ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Surface Display ◽  
Recombinant Baculovirus ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Challenge Study ◽  
Ifn Γ ◽  
Cellular Immune

Abstract To increase expression levels of the PCV2 Cap(d41) protein, novel baculovirus surface display vectors with multiple expression cassettes were constructed to create recombinant baculoviruses BacSC-Cap(d41), BacDD-2Cap(d41), BacDD-3Cap(d41), and BacDD-4Cap(d41). Our results reveal that the recombinant baculovirus BacDD-4Cap(d41) was able to express the highest levels of Cap(d41) protein. Optimum conditions for expressing the PCV2 Cap(d41) protein were determined, and our results show that 107 of Sf-9 infected with the recombinant baculovirus BacDD-4Cap(d41) at an MOI of 5 for 3 days showed the highest level of protein expression. Mice immunized with the 4Cap(d41) vaccine which was prepared from the recombinant baculovirus-infected cells (107) elicited higher ELISA titers compared to the Cap (d41) vaccine. The 4Cap(d41) vaccine could elicit anti-PCV2 neutralizing antibodies and IFN-γ in mice, as confirmed by virus neutralization test and IFN-γ ELISA. Moreover, the swine lymphocyte proliferative responses indicated that the 4Cap(d41) vaccine was able to induce a clear cellular immune response. Flow cytometry analysis showed that the percentage of CD4+ T cells and CD4+/CD8+ ratio was increased significantly in SPF pigs immunized with the 4Cap(d41) vaccine. Importantly, the 4Cap(d41) vaccine induced an IFN-γ response, further confirming that its effect is through cellular immunity in SPF pigs. An in vivo challenge study revealed that the 4Cap(d41) and the commercial vaccine groups significantly reduce the viral load of vaccinated pigs as compared with the CE negative control group. Taken together, we have successfully developed a 4Cap(d41) vaccine that may be a potential subunit vaccine for preventing the disease associated with PCV2 infections.

scholarly journals Evaluation of Adjuvant Effectiveness of Alum-Propranolol Mixture on the Immunogenicity of Excreted/Secreted Antigens of Toxoplasma gondii RH Strain

2020 ◽  
Vol 11 (3) ◽  
pp. 570-577
Author(s):  
Elyar Meshkini ◽  
Arash Aminpour ◽  
Khosrow Hazrati Tappeh ◽  
Shahram Seyyedi ◽  
Meysam Shokri
Keyword(s):  
Toxoplasma Gondii ◽  
Immune Responses ◽  
Negative Control Group ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Control Group ◽  
Cellular Immune Responses ◽  
Ifn Γ ◽  
Cellular Immune ◽  
Phosphate Buffered Saline

Purpose: The introduction of novel adjuvants is an important step in attempts to develop a safe and more efficient vaccine. The present study was performed to determine whether the use of a mixed beta-adrenergic receptor antagonist propranolol (PRP) and aluminum (alum), as an adjuvant, have efficacy for Toxoplasma gondii (T. gondii) vaccine to induce protective immunity in a mouse model. Methods: Female BALB/c mice divided into five groups were immunized with excretory-secretory antigens (ESA) vaccine, alum-ESA vaccine, PRP-ESA vaccine, and alum-PRP ESA vaccine, as well as with phosphate buffered saline (PBS), as a negative control group. The immune responses were evaluated by lymphocyte proliferation assay for measuring delayed-type hypersensitivity (DTH) response and by cytokine assay for evaluating IFN-γ and IL-5 levels. The survival rate of mice in all groups was assessed during a three-week monitoring period after an intraperitoneal challenge with T. gondii tachyzoites. Results: The results showed that mice immunized with PRP, as an adjuvant, could secret a higher level of IFN-γ, which was significant in comparison to other groups. However, mice vaccinated with alum-precipitated ESA antigen had ability to produce an elevated level of IL-5 compared to other mouse groups (p≤0.05). Moreover, alum-PRP co-administration together with ESA vaccine resulted in the longer survival of mice. Conclusions: The findings of this study revealed that the combination of alum-PRP adjuvants and ESA vaccine of T. gondii elicits both humoral and cellular immune responses, which are comparable to either alum or PRP alone.

scholarly journals Therapeutic effect of nanoliposomal PCSK9 vaccine in a mouse model of atherosclerosis

BMC Medicine ◽  
2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Amir Abbas Momtazi-Borojeni ◽  
Mahmoud Reza Jaafari ◽  
Ali Badiee ◽  
Maciej Banach ◽  
Amirhossein Sahebkar
Keyword(s):  
Therapeutic Effect ◽  
Plasma Concentrations ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Poloxamer 407 ◽  
Control Group ◽  
Igg Antibody ◽  
Lipid Film ◽  
Ifn Γ

Abstract Background Proprotein convertase subtilisin/kexin 9 (PCSK9) is an important regulator of low-density lipoprotein receptor (LDLR) and plasma levels of LDL cholesterol (LDL-C). PCSK9 inhibition is an efficient therapeutic approach for the treatment of dyslipidemia. We tested the therapeutic effect of a PCSK9 vaccine on dyslipidemia and atherosclerosis. Methods Lipid film hydration method was used to prepare negatively charged nanoliposomes as a vaccine delivery system. An immunogenic peptide called immunogenic fused PCSK9-tetanus (IFPT) was incorporated on the surface of nanoliposomes using DSPE-PEG-maleimide lipid (L-IFPT) and adsorbed to Alhydrogel® (L-IFPTA+). The prepared vaccine formulation (L-IFPTA+) and empty liposomes (negative control) were inoculated four times with bi-weekly intervals in C57BL/6 mice on the background of a severe atherogenic diet and poloxamer 407 (thrice weekly) injection. Antibody titers were evaluated 2 weeks after each vaccination and at the end of the study in vaccinated mice. Effects of anti-PCSK9 vaccination on plasma concentrations of PCSK9 and its interaction with LDLR were determined using ELISA. To evaluate the inflammatory response, interferon-gamma (IFN-γ)- and interleukin (IL)-10-producing splenic cells were assayed using ELISpot analysis. Results L-IFPTA+ vaccine induced a high IgG antibody response against PCSK9 peptide in the vaccinated hypercholesterolemic mice. L-IFPTA+-induced antibodies specifically targeted PCSK9 and decreased its plasma consecration by up to 58.5% (− 164.7 ± 9.6 ng/mL, p = 0.0001) compared with the control. PCSK9-LDLR binding assay showed that generated antibodies could inhibit PCSK9-LDLR interaction. The L-IFPTA+ vaccine reduced total cholesterol, LDL-C, and VLDL-C by up to 44.7%, 51.7%, and 19.2%, respectively, after the fourth vaccination booster, compared with the control group at week 8. Long-term studies of vaccinated hypercholesterolemic mice revealed that the L-IFPTA+ vaccine was able to induce a long-lasting humoral immune response against PCSK9 peptide, which was paralleled by a significant decrease of LDL-C by up to 42% over 16 weeks post-prime immunization compared to control. Splenocytes isolated from the vaccinated group showed increased IL-10-producing cells and decreased IFN-γ-producing cells when compared with control and naive mice, suggesting the immune safety of the vaccine. Conclusions L-IFPTA+ vaccine could generate long-lasting, functional, and safe PCSK9-specific antibodies in C57BL/6 mice with severe atherosclerosis, which was accompanied by long-term therapeutic effect against hypercholesterolemia and atherosclerosis.

scholarly journals Transfection of STAT3 Overexpression Plasmid Mediated by Recombinant Lentivirus Promotes Differentiation of Bone Marrow Mesenchymal Stem Cells Into Neural Cells in Fetal Rats With Spina Bifida Aperta

2021 ◽  
Author(s):  
mingyu Jiang ◽  
Ji-cheng Dai ◽  
Ming-ying Yin ◽  
Ming-yong Ren
Keyword(s):  
Spina Bifida ◽  
Neuron Specific Enolase ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Caspase 8 ◽  
Neural Cells ◽  
Blank Control Group ◽  
Spina Bifida Aperta ◽  
Experimental Group

Abstract Background To investigate the influence of signal transducer and activator of transcription-3 (STAT3) on spinal cord tissue grafts of rat fetuses with spina bifida aperta. In particular, we wished to determine if STAT3 overexpression plasmid transfection would increase survival of spinal cord transplantation to improve therapeutic efficiency. Methods The fetal rat model of spina bifida aperta was established by retinoic acid and treated by microsurgical injection of stem cells. They were divided into blank control group (n = 12), negative control group (n = 10) and experimental group (n = 11). The optical density (OD) value of BMSCs viability was determined by Cell Counting Kit-8 (CCK-8). The expression of STAT3, pSTAT3, nerve markers (Glial fibrillary acidic protein, Neuron-specific enolase, Neurofilament and Nestin) and apoptosis related factors (Caspase 8 and Bcl-2) were tested by the method of real-time PCR and Western blot. All statistical analyses were performed using SPSS version 20.0 software. One-way analysis of variance was used to analyze differences among three or more groups. The data are presented as the mean ± standard deviation from at least three independent experiments. P < 0.05 was considered statistically significant. Results OD value in experimental group was the highest at 8 hours after transplantation by CCK-8, and then gradually decreased, which was statistically significant compared with blank control group and negative control group (P < 0.05). There was no statistical difference in OD peak time and value between blank control group and negative control group. The expression levels of pSTAT3, Glial fibrillary acidic protein, Neuron-specific enolase, Neurofilament and Nestin in experimental group were remarkably higher than those in blank control group and negative control group (P < 0.05), but STAT3 expression in experimental group were statistically decreased (P < 0.05). The relative expression levels of Caspase-8 and Bcl-2 in experimental group were tremendously lower than those in blank control group and negative control group (P < 0.05). Conclusion Transfection of recombinant lentivirus mediated STAT3 overexpression plasmid with BMSCs can improve the efficiency of transforming into neural cells and provide new seed cells for the treatment of congenital spina bifida aperta.

A tablet derived from Andrographis paniculata complements dihydroartemisinin-piperaquine treatment of malaria in pregnant mice

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bastiana ◽  
Aty Widyawaruyanti ◽  
Hilkatul Ilmi ◽  
Lidya Tumewu ◽  
Budi Prasetyo ◽  
...  
Keyword(s):  
Negative Control ◽  
Andrographis Paniculata ◽  
Lower Percentage ◽  
Control Group ◽  
Suitable Alternative ◽  
Tnf Α ◽  
Malaria During Pregnancy ◽  
Ifn Γ

Abstract Objectives The use of standard antimalarial drugs, such as dihydroartemisinin-piperaquine (DHP) for the treatment of malaria during pregnancy is limited due to the risk of teratogenicity. The alternative is therefore required although few exist. Here we show a phytopharmaceutical drug derived from Andrographis paniculata (AS201-01), which is effective as herbal antimalarial both in vitro and in vivo and may be a suitable alternative when used in complementary treatment with DHP. Methods Plasmodium berghei infected pregnant BALB/c mice were divided into four groups: G1 (negative control), G2 (AS201-01), G3 (DHP), and G4 (combination of DHP and AS201-01). Pheripheral blood was collected during therapy for counting parasitemia. Placental samples were analyzed for the expression of IFN-γ, TNF- α, IL-10, placental parasite counts and foetal morphology. Results Groups G4 and G3 both showed a 100% inhibition of peripheral parasitemia. However, the treatment in G4 was found to be less effective than that in G2 and G3 in preventing placental parasitemia. The G4 treatment was able to reduce the expression of IFN-γ and IL-10, whereas TNF-α was not significantly different from the control group. Foetal morphologic abnormalities were observed in all groups except G2; G4 showed lower percentage of abnormalities compared to G3 and G1. Conclusions A combination of A. paniculata tablet (AS201-01) with DHP has the potential to reduce the toxicity of DHP in malaria treatment.

Effects of Radix Platycodonis Polysaccharide on Expressions of IFN-γ, IL-4 and IL-5 in the Bronchoalveolar Lavage Fluid and Nf-κВ in the Lung Tissue of Asthmatic Rats

2013 ◽  
Vol 750-752 ◽  
pp. 1549-1554
Author(s):  
Xiao Dong Huang ◽  
Xing Yu Zhao ◽  
Yan Chun Wang ◽  
Jian Ying
Keyword(s):  
Lavage Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Dose ◽  
Model Group ◽  
Blank Control Group ◽  
Asthma Model ◽  
Ifn Γ ◽  
Group 1

Objective: To investigate the effect of Radix playtycodoni polysaccharide (RPPS) on rats with ovalbumin-induced asthma and its mechanisms. Methods: 48 Wistar rats were randomly divided into blank control group, asthma model group, dexamethasone control group (1 mg / kg), and high-dose RPPS group (40 mg / kg), moderate-dose RPPS group (20 mg / kg) and low-dose RPPS group (10 mg / kg). The ovalbumin sensitization method was used to establish the asthma model in rats. The rats were given it in the atomization administration for two weeks and then were sacrificed. The BALF was collected for the count of eosinophils (EOS); enzyme-linked immunosorbent assay (ELISA) method was employed for the determination of IL-4, IL-5, and IFN-γ levels in BALF; the expression of NF-κВ in the lung tissues was measured by Western blot. Results: Compared with the model group, RPPS could reduce the number of EOS in blood and BALF, increase the content of IFN-γ in BALF, lower IL-4 and IL-5 contents, and down-regulate the expression of NF-κВ protein in rats with asthma. Conclusion: RPPS can inhibit the airway inflammation in asthma, down-regulate the expression level of IL-4 and IL-5, up-regulate the IFN-γ level to regulate the abnormal immune status induced by the imbalance of TH1/TH2 in asthma and inhibit the expression of NF-κВ, which may be one of the mechanisms of its anti-inflammatory activity.

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