scholarly journals Influence of Geographic Origin and Plant Source on Physicochemical Properties, Mineral Content, and Antioxidant and Antibacterial Activities of Moroccan Propolis

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Naoual El Menyiy ◽  
Meryem Bakour ◽  
Asmae El Ghouizi ◽  
Soukaina El Guendouz ◽  
Badiaa Lyoussi
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Mineral Content ◽  
Biological Activities ◽  
Geographic Origin ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Mineral Contents ◽  
Linear Discriminant

This research is aimed at determining the physicochemical properties (resin, wax, balsams, pH, moisture, ash, and mineral contents) of propolis samples collected from different geographical areas in Morocco, as well as evaluating the antioxidant and antibacterial activities of these samples. The results showed the following values for physicochemical analysis: resin (17.42-58.01%), wax (21.31-70.12%), balsam (0.27-2.12%), pH (3.7-5.3), moisture (1.02-3.65%), and ash (0.72-5.01%). The phenolic and flavone/flavonol contents of samples were ranged between 6.74 mg FAE/g and 149.13 mg FAE/g and between 1.19 mg QE/g and 108.11 mg QE/g, respectively. The sample P3 presented also the strongest radical scavenging activity toward DPPH, ABTS free radicals, and FRAP assay with IC50 values of 0.021, 0.026, and 0.042 mg/mL, respectively. All propolis samples showed significant inhibitory effects against all tested microorganisms with MICs ranging from 0.28 mg/mL to 1.12 mg/mL for Gram-negative strains and from 0.002 mg/mL to 1.12 mg/mL for Gram-positive strains. A strong correlation was found between resin, total phenolic compounds, flavones/flavonols, and antioxidant activity. Linear discriminant analysis revealed that the samples studied were divided into two groups which were differentiated by the data of antioxidant activity, mineral contents, and antibacterial activity. It can be concluded that the physicochemical properties, mineral content, and biological activities of Moroccan propolis depend on their geographical and botanical origin.

scholarly journals Physicochemical Properties and Biological Activity of Active Films Based on Corn Peptide Incorporated Carboxymethyl Chitosan

Coatings ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 604
Author(s):  
Liyan Wang ◽  
Liang Lei ◽  
Kang Wan ◽  
Yuan Fu ◽  
Hewen Hu
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Food Packaging ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Carboxymethyl Chitosan ◽  
Vapor Permeability ◽  
Zone Method ◽  
Oil Resistance

Active films based on carboxymethyl chitosan incorporated corn peptide were developed, and the effect of the concentration of corn peptide on films was evaluated. Physicochemical properties of the films, including thickness, opacity, moisture content, color, mechanical properties, water vapor permeability, and oil resistance, were measured. Biological activities of the films, including the antioxidant and antibacterial activities, were characterized in terms of 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging activity, reducing power, the total antioxidant activity, and the filter disc inhibition zone method. The results indicated that the incorporation of corn peptide caused interactions between carboxymethyl chitosan and corn peptide in Maillard reaction and gave rise to the films light yellow appearance. Compared with the Control, the degree of glycosylation, browning intensity, thickness, opacity, tensile strength, antioxidant activity, and antibacterial activity of films were increased, but the elongation, vapor permeability, and oil resistance of films were decreased. The films based on corn peptide and carboxymethyl chitosan can potentially be applied to food packaging.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

scholarly journals Free-radical Scavenging and Quantitative Estimation of Flavonoids from the Polar Extracts of Corchorus depressus Leaves

2021 ◽  
pp. 210-219
Author(s):  
Khuntia Tapas Kumar ◽  
Nanda Upendra Nath ◽  
Senapati Aswini Kumar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Nitric Oxide Radical ◽  
Polar Extracts

Background: The investigation of total flavonoids and antioxidant activity of polar extracts of Corchorus depressus is the major aim of this study. As observed from ancient literatures and folkloric claims the plant Corchorus depressus worshipped by the married women of Odisha, India, in the rituals called as “Jama Jutia”, possesses different biological activities including antioxidant property. Methods: The diphenyl picryl hydrazine, hydroxyl radical and nitric oxide radical scavenging methods were performed for measurement of the antioxidant activity at different extracts. The flavonoid and phenolic content of the extracts were determined by using aluminium chloride and Folin-Ciocalteau’s reagent (FCR) methods respectively. Results: The results for estimation of total phenolic content (mg/ 100 g) expressed as gallic acid equivalent (GAE) and total flavonoid (mg/ 100 g) in weight of quercetin equivalent (QE) was highest in methanolic extract 78.46 and  21.2 respectively, followed by 18.18 mg/100 g in GAE and 1.80 mg/100 g in QE for aqueous extract. Conclusion: The methanolic extract of C. depressus at 100µg/ml showed highest DPPH, hydroxyl and nitric oxide radical scavenging activity and this activity may be attributed to the presence of saponins and flavonoids as detected in the extract.

scholarly journals Antioxidant, Cytotoxic, and Antibacterial Activities of the Selected Tibetan Formulations Used in Gandaki Province, Nepal

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Madan Dhakal ◽  
Prakash Poudel ◽  
Upma Jha ◽  
Suresh Jaiswal ◽  
Khem Raj Joshi
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Effect ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Tibetan Medicine ◽  
Root Tip ◽  
Standard Drug ◽  
Traditional Medicines

Background and Objective. Tibetan medicine is one of the earliest-known traditional medicines. This study aimed to evaluate the antioxidant, cytotoxic, and antibacterial potential of ethanolic extracts of nine common Tibetan formulations. Materials and Methods. An open-ended and semistructured questionnaire was used for an ethnomedicinal survey of the Tibetan formulations practiced in four Tibetan refugee settlements in Gandaki Province, Nepal. Based on the ethnomedicinal survey data, commonly used nine formulations were selected (Aru-18, Basam, Dadue, Dashel, Mutik-25, Raab Ga Yangzin Tea, Serdok-11, Sugmel-10, and Yungwa-4) to test biological activities. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging method. The cytotoxicity was examined by using the Allium cepa L. root tip meristem model. Similarly, the antibacterial effect was assessed by using well diffusion and broth dilution methods. Results. An ethnomedicinal survey showed a total of 52 Tibetan formulations were generally used by respondents for common diseases such as stomach disorders, diabetes, and migraine. From the antioxidant activity test, Sugmel-10 showed the highest DPPH free-radical-scavenging activity (IC50 1.8 μg/ml) and Yungwa-4 showed the lowest activity (IC50 5.2 μg/ml). Also, from the cytotoxic activity, the A. cepa root meristem model exhibited significant dose- and time-dependent growth suppression in Basam, Dadue, Mutik-25, and Serdok-11 as compared with cyclophosphamide standard drug. Similarly, Basam showed a good antibacterial effect having MIC 20 mg/ml and MBC 100 mg/ml against Enterococci faecalis. Conclusion. Research showed that Tibetan people preferred Tibetan formulations for the treatment and mitigation of several diseases. The result of antioxidant, cytotoxic, and antibacterial activities experimentally justified the ethnomedicinal value of nine common formulations (Aru-18, Basam, Dadue, Dashel, Mutik-25, Raab Ga Yangzin Tea, Serdok-11, Sugmel-10, and Yungwa-4). To the best of our knowledge, this study was performed for the first time in Nepal. Results from this preliminary study open the door to the scientific world to perform extensive pharmacological studies for designing and developing new therapeutic agents.

Antioxidant and antifungal activities of Myrianthus arboreus P. Beauv. (Moraceae), Allanblackia gabonensis Pellegr. (Clusiaceae) and three other Cameroonian medicinal plants

2021 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Aimé G. Fankam ◽  
Serge S. Atsafack ◽  
Guy S. S. Njateng ◽  
Jules R. Kuiate
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Medicinal Plants ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Bark Extract ◽  
Total Phenolic ◽  
Antifungal Activities ◽  
Allanblackia Gabonensis

Background: Biological activities of medicinal plants make them attractive alternative complementary medicines. Therefore, this study aimed to assess the antioxidant and antifungal activities of the methanol extract of Myrianthus arboreus, Allanblackia gabonensis, Gladiolus quartinianus, Peperomia fernandopoiana and Vepris soyauxii. Methods: Total phenolic contents (TPC) and flavonoid contents (FC) were evaluated using standard methods. The antioxidant activity of the extracts was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing power assays. Antifungal activity was evaluated by the broth micro-dilution method. Results: TPC of extracts ranging from 67.89 to 196.01 mgGAE/g TPC were very higher in P. fernandopoiana whole plant (PFW) extract (196.01±10.25 mgGAE/g) and MAL (165.71±10.68 mgGAE/g) whereas the A. gabonensis fruit extract (AGF) and M. arboreus bark extract (MAB) had the highest flavonoid content (66.54 ± 2.12 and 48.13 ± 1.65 mgEC/g of extract, respectively). Apart from M. arboreus leave extract (MAL), all extracts showed important DPPH radical scavenging activity (IC50 < 20 μg/mL). AGFl and PFW exhibited significant ferric reducing power compared to ascorbic acid and other extracts (P<0.05). Each of the tested extracts showed antifungal activity against atleast one of the five pathogenic fungi, with MICs ranging from 128 to 1024 µg/mL. MAL was active on all the tested fungi whereas MAB showed the highest antifungal activity (MIC=128 µg/mL). Conclusion: The findings of this study emphasize the evidence that the methanol extracts of the leave and bark of Myrianthus arboreus could be an alternative to fight against oxidative stresses and infections due to opportunistic yeast pathogens. Keywords: Oxidative stress; fungal infection; medicinal plant; antioxidant activity; antifungal activity.

An investigation on Trakya region Oak (Quercus spp.) honeys of Turkey: their physico-chemical, antioxidant and phenolic compounds properties

2018 ◽  
Vol 43 (4) ◽  
pp. 362-374 ◽  
Author(s):  
Sevgi Kolayli ◽  
Zehra Can ◽  
Hilal Ebru Çakir ◽  
Onur Tolga Okan ◽  
Oktay Yildiz
Keyword(s):  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Optical Rotation ◽  
Radical Scavenging ◽  
High Ratio ◽  
Total Phenolic ◽  
Mineral Contents ◽  
Antioxidant Power ◽  
Phenolic Components ◽  
Ferric Reducing Antioxidant Power

Abstract Objective The purpose of this study was therefore to investigate various physicochemical properties, phenolic composition and antioxidant capacities of the oak honey, large quantities of which are produced in the Thrace region of Northwest Turkey. Methods Hunter color values (Lab), optical rotation, electrical conductivity, moisture and ash were measured as physicochemical determinants. Sugar, proline content, mineral, phenolic acids, total phenolic flavonoids contents were evaluated as chemical parameters. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging were used as antioxidant determinants. Results The results determined that dark-colored honeys (mean 24.95±8.35 L) have proline levels (649.82±203.90 mg/kg), total phenolic content (TPC) (67.29±13.10 mgGAE/100 g) and total flavonoid content (TFC) (10.14±4.78 mgQE/100 g), a high ratio of fructose/glucose (1.65±0.18). Rutin (11.14±8.50 μg/g), p-coumaric acid (6.97±5.80 μg/g) and protocatechuic acid (6.12±4.39 μg/g) were the major phenolic components, and quercetin was also detected in varying amounts in all samples. Potassium (2523±0.28 mg/kg), calcium (63.40±3.20 mg/kg) and magnesium (38.88±0.65 mg/kg) were the major minerals in the honeys. Conclusion Our results showed that physicochemical properties, phenolic compounds, antioxidant capacity, sugar and mineral contents successfully distinguish the geographical origins of oak honey. These findings will contribute to a more accurate evaluation of oak honey in the literature.

scholarly journals Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4589
Author(s):  
Soumaya Touzani ◽  
Hamada Imtara ◽  
Shankar Katekhaye ◽  
Hamza Mechchate ◽  
Hayat Ouassou ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

scholarly journals Chemical characterization, fatty acid profile and antioxidant activity of Gustavia macarenensis fruit mesocarp and its oil from the Amazonian region of Ecuador as an unconventional source of vegetable oil

2019 ◽  
Vol 70 (2) ◽  
pp. 298
Author(s):  
J. J. Reyes Mera ◽  
R. Abreu-Naranjo ◽  
J. M. Alvarez-Suarez ◽  
D. Viafara
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Total Phenolic ◽  
Linear Discriminant ◽  
Chromatography Analysis

To our knowledge, this study is the first to report on the nutritional characterization, bioactive compounds and antioxidant activity of Amazonian G. macarenensis fruit. The fatty acid profile was determined using a high performance liquid chromatography analysis. The total phenolic content and antioxidant activity were determined using Folin Ciocalteu’s method and by radical scavenging activity, respectively. Moreover, a cluster analysis was carried out in order to classify the G. macarenensis fruit oil according to its fatty acid profile. Seven Fisher linear discriminant functions were obtained from the discriminant analysis. These models allow one to classify new fruits on the basis of their fatty acid profile. A high value for total lipids was obtained (53.57%). Its main components were palmitic and oleic acid. The TPC value (156.49 ± 2.62 mg GAE/Kg of oil) obtained from the G. macarenensis fruit pulp oil is higher than what was reported for some olive oil and Brazilian mango oil varieties.

scholarly journals Extraction of Anthocyanins from Borage (Echium amoenum) Flowers Using Choline Chloride and a Glycerol-Based, Deep Eutectic Solvent: Optimization, Antioxidant Activity, and In Vitro Bioavailability

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 134
Author(s):  
Oscar Zannou ◽  
Hojjat Pashazadeh ◽  
Mohamed Ghellam ◽  
Salam A. Ibrahim ◽  
Ilkay Koca
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Choline Chloride ◽  
Radical Scavenging ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Power ◽  
Echium Amoenum

Borage flower (Echium amoenum), an annual herb native to the Mediterranean region, is an excellent source of anthocyanins and is widely used in various forms due to its biological activities. In the present study, a choline chloride and glycerol (CHGLY)-based natural deep eutectic solvent (NADES) was applied in order to extract the anthocyanins from borage flowers. The traditional solvents, including water, methanol, and ethanol, were used to evaluate the efficiency of CHGLY. The results showed that CHGLY was highly efficient compared to the traditional solvents, providing the highest amounts of the total anthocyanin content (TAC), total phenolic content (TPC), total flavonoid content (TFC), individual anthocyanins, and antioxidant activity (DPPH radical scavenging (DPPH) and ferric-reducing antioxidant power (FRAP) assays). The most dominant anthocyanin found in studied borage was cyanidin-3-glucoside, followed by cyanin chloride, cyanidin-3-rutinoside, and pelargonidin-3-glucoside. The bioavailability % was 71.86 ± 0.47%, 77.29 ± 0.57%, 80.22 ± 0.65%, and 90.95 ± 1.01% for cyanidin-3-glucoside, cyanidin-3-rutinoside, by pelargonidin-3-glucoside and cyanin chloride, respectively. However, cyanidin-3-glucoside was the anthocyanin compound showing the highest stability (99.11 ± 1.66%) in the gastrointestinal environment. These results suggested that choline chloride and glycerol-based NADES is not only an efficient, eco-friendly solvent for the extraction of anthocyanins but can also be used to increase the bioavailability of anthocyanins.

scholarly journals Biological Value of Centaurea damascena: Minireview

2019 ◽  
Vol 5 (2) ◽  
pp. 99-106
Author(s):  
Mohammad Jaafreh ◽  
Haitham Qaralleh ◽  
Muhamad Al-limoun
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antibacterial Activities ◽  
Essential Oil Composition ◽  
Total Phenolic ◽  
Oil Composition ◽  
Phenolic Contents ◽  
The Family

The family Asteraceae include large number of Centaurea species which have been applied in folk  medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial activity. The aim of the study was to discuss antibacterial activities of essential oil composition and methanolic extract of the same plant aerial part leaves.  Thirty-seven components were characterized with 86% of oxygenated terpenes. The composition was dominated by 11.45% Fokienol, 8.8% thymol, 8.21% Alpha Terpineol, 7.24% Chrysanthemumic acid, 7.13% Terpinen-4-ol and 6.59% Borneol with a high degree of polymorphism in the occurrence of these compounds as compared with the different species of centaurea.. Free radical scavenging capacity of the C. damascna methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent. 

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