scholarly journals Preparation and Characterization of Polyamidoamine G2.0-Hematin as a Biocatalyst for Fabricating Catecholic Gelatin Hydrogel

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Tan Phuoc Ton ◽  
Thi Yen Nhi Tran ◽  
Le Hang Dang ◽  
Kim Thi Hoang Nguyen ◽  
Phuong Doan ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
In Vitro Study ◽  
Pamam Dendrimer ◽  
Relative Activity ◽  
Oxidation Reactions ◽  
Enzyme Mimicking ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

In this study, we report that an enzyme-mimicking biocatalyst polyamidoamine (PAMAM) dendrimer G2.0-hematin (G2.0-He) was fabricated successfully. The chemical structure of G2.0-He was verified by 1H NMR and FT-IR spectroscopy. G2.0-He exhibited a size distribution from 11.6 ± 1.7   nm to 12.5 ± 2.9   nm and a zeta potential from 32.5 mV to 25.6 mV along with the enhancement of the hematin conjugation degree. The relative activity of G2.0-He was evaluated based on pyrogallol oxidation reactions at pH = 7 . The results showed that G2.0-He was more stable than horseradish peroxidase (HRP) enzyme in high H2O2 concentrations. The HRP-mimic ability of G2.0-He was also confirmed by the catalyzation when preparing catecholic gelatin hydrogels under mild conditions. Moreover, our results also revealed that these hydrogels performed with excellent cytocompatibility in an in vitro study and could be used as a potential scaffold for adhesion and proliferation of fibroblast cells. The obtained results indicated that G2.0-He is a suitable platform for altering the HRP enzyme in several biomedical applications.

scholarly journals Characterization of Chitosan from Shell of Snail (Achatina Fulica F) and Its Antibacterial Activity againts Staphylococcus aureus

Biota ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 22-31
Author(s):  
Umar Umar ◽  
Surahmaida Surahmaida ◽  
Rio Alta ◽  
Rizka Surya Ningrum
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Deacetylation Degree ◽  
Ft Ir ◽  
Amine Groups ◽  
One Way Anova ◽  
Test Result ◽  
Ft Ir Spectroscopy

The shell of snail contains chitin that can be transformed into chitosan through four stages, which are deproteination, demineralization, depigmentation, and deacetylation. Chitosan can be utilized as an antibacterial agent because it has amine groups. In this research, chitosan that prepared from the snail shell will be analyzed using FT-IR spectroscopy to determine the deacetylation degree (DD). The antibacterial activity test result of Staphylococcus aureus will be carried out on chitosan with various concentrations using in-vitro Kirby Bauer method and will be analyzed using One Way ANOVA with confidence level at 95%. The variations concentration of chitosan used is 300, 400, 500, 600, and 700 ppm. The result shows that the deacetylation degree of chitosan is 63.09% and the optimum concentration of chitosan that can inhibit the Staphylococcus aureus bacteria is 500 ppm with diameter inhibition is 34.33 mm.

An In Vitro Study on Inhibiting Pathogenic Activity by Biosynthesized Silver Nanoparticles using Dioscorea Pentaphylla

2021 ◽  
Vol 14 (2) ◽  
pp. 5412-5423
Author(s):  
Muniyappan N ◽  
Murugan Murugapandi
Keyword(s):  
Silver Nanoparticles ◽  
In Vitro Study ◽  
Nano Particles ◽  
Biological Synthesis ◽  
Morphological Studies ◽  
Nanoparticles Concentration ◽  
Phenolic Group ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Development of biologically stimulated experimental processes for the biosynthesis of nanoparticles is an essential branch of nanobiotechnology. Biocompatible silver nanoparticles have  acknowledged  significant notice in recent years because of their capable applications in bioimaging, biosensors, biolabels, and biomedicine. This study reports a green chemistry approach for the biological synthesis of silver nanoparticles by using the rhizomes extract of Dioscorea Pentaphylla. The properties of synthesis nanoparticles were characterized by UV-Visible spectroscopy, FT-IR, SEM, TEM and EDS. Surface Plasmon Resonance (SPR) spectrum for silver nano-particles is obtained at 426 with dark brown color. FT-IR spectroscopy revealed   that silver nanoparticles were functionalized with bio- molecules that have primary amine group (NH2), carbonyl phenolic group (-OH) and other stabilizing functional groups. The Morphological studies revealed that the particles are spherical in shape and in the size ranged from 20 ± 1nm. The effects of silver nanoparticles concentration and extract concentration  were investigated and DPR-AgNPs showed the better pharma- cological applications.

Preparation and Characterization of Nano-β-Tricalcium Phosphate/Silk Fibroin Scaffold for Tissue Engineering Applications

2011 ◽  
Vol 343-344 ◽  
pp. 882-888
Author(s):  
Jun Ou ◽  
Yu Min Jiang ◽  
Zhan He Zhang
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Tricalcium Phosphate ◽  
Biomedical Applications ◽  
3D Scaffold ◽  
Ft Ir ◽  
Ir Analysis ◽  
Cultivation Experiment

Silk fibroin (SF) and β-tricalcium phosphate (β-TCP) had been used in biomedical applications for these years. The potential of silk and β-TCP for application in tissue engineering is currently being explored. The purpose of this study was to prepare and characterize a 3D scaffold consisting of nano-β-TCP/SF composite. XRD and FT-IR analysis showed that predominant crystalline phase of calcium phosphate was β-TCP; a chelate effect between SF and Ca2+ was happened at complexing period of SF and β-TCP. The compressive strength of nano-β-TCP/SF composite was 42 MPa ± 0.12 MPa. In vitro cell cultivation experiment showed that the composite was a good matrix for the growth of osteoblasts. Conclusion: the incorporation of SF into nano-β-TCP can enhance both mechanical strength and bioactivity of the scaffold, which suggests that the β-TCP/SF composite may be a potential biomaterial for tissue engineering.

Photoacoustic FT-IR Spectroscopy of Natural Copper Patina

1995 ◽  
Vol 49 (10) ◽  
pp. 1516-1524 ◽  
Author(s):  
Alex O. Salnick ◽  
Werner Faubel
Keyword(s):  
Depth Distribution ◽  
Depth Profiling ◽  
Beam Deflection ◽  
City Hall ◽  
Spectral Bands ◽  
Special Sample Preparation ◽  
Ft Ir ◽  
Infrared Photoacoustic Spectroscopy ◽  
Ft Ir Spectroscopy

Fourier transform infrared photoacoustic spectroscopy (FT-IR/PAS) has proved to be a useful tool for nondestructive testing of copper corrosion layer (patina) formed in the atmosphere. The samples cut from a piece of the roof of the Stockholm City Hall were examined without any additional pretreatment. The components of the patina—brochantite Cu4(OH)6SO4, antlerite Cu3(OH)4SO4, and basic cupric carbonate Cu2CO3(OH)6 · H2O—as well as some other minerals were identified. The photothermal beam deflection (PBD) method was used for independent photoacoustic characterization of the samples. The depth profiling capability of FT-IR/PAS was used to determine the degree of photoacoustic saturation of the spectral bands and to evaluate the depth distribution of the main patina components. The technique thus compares favorably with more common approaches of patina examination which are more expensive and require special sample preparation.

Nano-Sized [60]Fullerene-Cyclodextrin Molecules

2001 ◽  
Vol 675 ◽  
Author(s):  
Jeong-Seo Park ◽  
Han-Chang Kang ◽  
Kurt E. Geckeler
Keyword(s):  
Biomedical Applications ◽  
Addition Reaction ◽  
Water Soluble ◽  
Separation And Purification ◽  
Subsequent Reaction ◽  
Fullerene Derivatives ◽  
Covalently Bonded ◽  
Scattering Measurements ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

ABSTRACTAs [60]fullerene is a very hydrophobic macromolecule, there have been a number of attempts to make it more hydrophilic for biomedical applications. By attaching hydrophilic moieties such as poly(oxyethylene)(POE) chains and cyclodextrin molecules to [60]fullerene, novel water-soluble and biocompatible materials have been successfully prepared [1,2].The synthesis of novel macrocyclic fullerene conjugates which are water-soluble is reported. The telechelic fullerene derivatives have been prepared via addition reaction of POE-based arms with covalently bonded β-cyclodextrin (CD) to [60]fullerene. To this end, a mono-tosylated CD derivative has been prepared in pyridine and then reacted with an amino-functional POE in the presence of triethylamine. The subsequent reaction of [60]fullerene with the hydrophilic POE-conjugated CD-derivative yielded the macrofullerene after separation and purification procedures.The macrocyclic [60]fullerene derivatives obtained were soluble in water and characterized by UV-VIS and FT-IR spectroscopy as well as light scattering measurements and thermogravimetric analysis.

FT-IR spectroscopy characterization of schwannoma: a case study

2016 ◽  
Author(s):  
Isabelle Ferreira ◽  
Lazaro P. M. Neto ◽  
Maurilio José das Chagas ◽  
Luís Felipe C. S. Carvalho ◽  
Laurita dos Santos ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Ft Ir ◽  
Ft Ir Spectroscopy
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