scholarly journals Screening of Novel Source for Genistein by Rapid and Sensitive UPLC-APCI-TOF Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Aparna Bettaiah ◽  
Hema Bommanamane Prabhushankar
Keyword(s):  
Extraction Method ◽  
Research Work ◽  
Solvent Mixture ◽  
Food Sources ◽  
The Novel ◽  
High Concentration ◽  
Simple Method ◽  
Limited Food ◽  
Solid Liquid ◽  
Tof Ms

Genistein has been shown to have a broad spectrum of health advantages. Only legumes were reported to have a significant amount of genistein with the highest concentration in Soybean. Soybean was found to cause allergies in children with atopic dermatitis and in adults. Limited food sources have hindered the use of genistein in daily diets, medications, and nutraceuticals. The main objective of the current research work was to discover the novel source for genistein by the simple method of extraction and quantification. Genistein was extracted by solid-liquid extraction technique. Extraction parameters were optimized by a single factor test. Identification and quantification of genistein from the selected seeds of Apiaceae were carried out using UPLC-APCI-TOF-MS. UPLC-APCI-TOF-MS method was successfully developed, validated (linearity ( R 2 = 0.999 ), precision (R.S.D. <5%), and accuracy (107.23%)), and used for the study. Remarkably, a high concentration of the genistein (811.57 μg/g) was found in the Cuminum cyminum. Solvent mixture (50 mL Methanol+25 mL Dimethyl sulphoxide+25 mL Water ( v / v / v )), temperature (80°C), and time (1 h) were found to be the optimum extraction conditions. The concentration of genistein before optimization was 226.67 μg/g and after optimization is 811.57 μg/g. This shows the efficiency of the extraction method in the extraction of genistein without the need for hydrolysis. Novel source for genistein is identified in regular human food can be consumed in a regular diet which increases wellness of human health along with enhancing the taste of the food. The developed extraction method coupled with high throughput, sensitive, and selective UPLC-APCI-TOF-MS technique facilitates rapid quantification (8 minutes of run time) without primary purification of complex extract.

scholarly journals Surface Modification of Magnetic Nanoparticles by Carbon-Coating Can Increase Its Biosafety: Evidences from Biochemical and Neurobehavioral Tests in Zebrafish

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2256
Author(s):  
Nemi Malhotra ◽  
Gilbert Audira ◽  
Jung-Ren Chen ◽  
Petrus Siregar ◽  
Hua-Shu Hsu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Photothermal Therapy ◽  
Research Work ◽  
Living Organism ◽  
The Novel ◽  
Adult Zebrafish ◽  
High Concentration ◽  
Biochemical Tests ◽  
Transmission Electron ◽  
Waterborne Exposure

Recently, magnetic nanoparticles (MNPs) have gained much attention in the field of biomedical engineering for therapeutic as well as diagnostic purposes. Carbon magnetic nanoparticles (C-MNPs) are a class of MNPs categorized as organic nanoparticles. C-MNPs have been under considerable interest in studying in various applications such as magnetic resonance imaging, photothermal therapy, and intracellular transportof drugs. Research work is still largely in progress for testing the efficacy of C-MNPs on the theranostics platform in cellular studies and animal models. In this study, we evaluated the neurobehavioral toxicity parameters on the adult zebrafish (Danio rerio) at either low (1 ppm) or high (10 ppm) concentration level of C-MNPs over a period of two weeks by waterborne exposure. The physical properties of the synthesized C-MNPs were characterized by transmission electron microscopy, Raman, and XRD spectrum characterization. Multiple behavior tests for the novel tank, mirror biting, predator avoidance, conspecific social interaction, shoaling, and analysis of biochemical markers were also conducted to elucidate the corresponding mechanism. Our data demonstrate the waterborne exposure of C-MNPs is less toxic than the uncoated MNPs since neither low nor high concentration C-MNPs elicit toxicity response in behavioral and biochemical tests in adult zebrafish. The approach combining biochemical and neurobehavioral approaches would be helpful for understanding C-MNPs association affecting the bioavailability, biosafety, interaction, and uptake of these C-MNPs in the living organism.

scholarly journals 4′-O-methylpyridoxine: Preparation from Ginkgo biloba Seeds and Cytotoxicity in GES-1 Cells

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 95
Author(s):  
Jin-Peng Zhu ◽  
Hao Gong ◽  
Cai-E Wu ◽  
Gong-Jian Fan ◽  
Ting-Ting Li ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Extraction Method ◽  
Mitochondrial Membrane ◽  
Gastric Epithelial Cells ◽  
Inhibition Rate ◽  
Liquid Ratio ◽  
High Concentration ◽  
Ic50 Value ◽  
G2 Phase ◽  
Solid Liquid

Ginkgo biloba seeds are wildly used in the food and medicine industry. It has been found that 4′-O-methylpyridoxine (MPN) is responsible for the poisoning caused by G. biloba seeds. The objective of this study was to explore and optimize the extraction method of MPN from G. biloba seeds, and investigate its toxic effect on human gastric epithelial cells (GES-1) and the potential related mechanisms. The results showed that the extraction amount of MPN was 1.933 μg/mg, when extracted at 40 °C for 100 min, with the solid–liquid ratio at 1:10. MPN inhibited the proliferation of GES-1 cells, for which the inhibition rate was 38.27% when the concentration of MPN was 100 μM, and the IC50 value was 127.80 μM; meanwhile, the cell cycle was arrested in G2 phase. High concentration of MPN (100 μM) had significant effects on the nucleus of GES-1 cells, and the proportion of apoptotic cells reached 43.80%. Furthermore, the Western blotting analysis showed that MPN could reduce mitochondrial membrane potential by increasing the expression levels of apoptotic proteins Caspase 8 and Bax in GES-1 cells. In conclusion, MPN may induce apoptosis in GES-1 cells, which leads to toxicity in the human body.

Soft Malleable Vesicles: versatile carriers for efficient topical delivery of fungal therapeutics

Drug Research ◽  
2020 ◽  
Author(s):  
Preeti Gupta ◽  
Antesh Kumar Jha ◽  
Mahesh Prasad ◽  
Poonam Kushwaha
Keyword(s):  
Topical Treatment ◽  
Healthcare Professionals ◽  
Fungal Infections ◽  
Topical Delivery ◽  
Systemic Circulation ◽  
Skin Permeability ◽  
Low Permeability ◽  
The Novel ◽  
High Concentration ◽  
Efficient Delivery

AbstractFungal infections have become a subject of great concern and the incidence of fungal infections is increasing, presenting an enormous challenge to healthcare professionals. Since most of the fungal infections are occurring over the skin, the treatment option of these infections always involves topical application. However, in topical delivery drug reaches into systemic circulation through different barriers of skin. Nevertheless, due to the low permeability, skin restricts the movement of many drugs. Hence, a delivery system is required, which deliver the medicament into the skin layers or through the skin and into the systemic circulation. Ethosomes or Soft malleable vesicles are the novel lipid vesicular carrier that offer improved skin permeability and efficient delivery due to their structure and composition. They contain high concentration of ethanol, which increases the fluidity of the skin. Therefore, in the present paper, we have explored the utility of ethosomal systems in the topical treatment of fungal infections. Structure, compositions types, mechanism and techniques of preparation of ethosome also discussed in the paper.

Utilizing an Adaptive Controller (Azadi Controller) for Trajectory Planning of PUMA 560 Robot

2011 ◽  
Vol 403-408 ◽  
pp. 4880-4887
Author(s):  
Sassan Azadi
Keyword(s):  
Steady State ◽  
Trajectory Planning ◽  
Positive Feedback ◽  
Fuzzy Controller ◽  
Research Work ◽  
Adaptive Controller ◽  
The Novel ◽  
Transient Responses ◽  
Simulation Results ◽  
Good Substitute

This research work was devoted to present a novel adaptive controller which uses two negative stable feedbacks with a positive unstable positive feedback. The positive feedback causes the plant to do the break, therefore reaching the desired trajectory with tiny overshoots. However, the two other negative feedback gains controls the plant in two other sides of positive feedback, making the system to be stable, and controlling the steady-state, and transient responses. This controller was performed for PUMA-560 trajectory planning, and a comparison was made with a fuzzy controller. The fuzzy controller parameters were obtained according to the PSO technique. The simulation results shows that the novel adaptive controller, having just three parameters, can perform well, and can be a good substitute for many other controllers for complex systems such as robotic path planning.

Directional manipulation of diffusio-osmosis flow by design of solute-wall and solvent-wall interactions

2021 ◽  
Author(s):  
Xin Wang ◽  
Dengwei Jing
Keyword(s):  
Critical Role ◽  
Liquid Interface ◽  
High Concentration ◽  
Interfacial Layers ◽  
Fluidic Devices ◽  
Wall Interaction ◽  
Dynamics Simulations ◽  
External Forces ◽  
Solid Liquid Interface ◽  
Solid Liquid

Abstract Understanding of the diffusio-osmosis, the flow induced by a solute gradient acting in narrow interfacial layers at nanoscale solid-liquid interface, is of great value in view of the increasing importance of micro- and nano-fluidic devices and self-propelling particle. Here, using molecular dynamics simulations, we develop a numerical method for direct simulation of diffusio-osmosis flows mimicking the realistic experiment without any assumed external forces. It allows us to obtain reliable flow details which is however hard to get in experiments. We found that the solvent-wall interaction, previously overlooked in classical paradigm, plays a critical role in diffusio-osmosis process. In particular, diffusio-osmosis is controlled by the interaction difference between solute-wall and solvent-wall. When solute-than solvent-wall, a surface excess (depletion) of solute particles on solid-liquid interface is formed which induces diffusio-osmosis flow towards low (high) concentration. We modified the classical Derjaguin expression to include the effect of nanoscale hydrodynamics boundary conditions for the accurate prediction of diffusio-osmosis characteristics. Overall, our results provide the clear guidance for controlling fluids flow and manipulating motion of colloids under tunable solute concentrations.

COMPARATIVE LITERATURE: EAST AND WEST STUDIES

2021 ◽  
Vol 02 (09) ◽  
pp. 8-14
Author(s):  
Aziza Komilovna Akhmedova ◽  
Keyword(s):  
Comparative Literature ◽  
Professional Ethics ◽  
Research Work ◽  
Literary Studies ◽  
The Novel ◽  
World Science ◽  
History Of ◽  
The Aesthetic ◽  
Aesthetic Ideal ◽  
The Ideal

The article analyzes the results of the research on the representation of the aesthetic ideal through the image of the ideal hero in two national literatures. For research purposes, attention was paid to highlighting the category of the ideal hero as an expression of the author's aesthetic views. In Sinclair Lewis’s “Arrowsmith” and Pirimkul Kodirov's “The Three Roots”, the protagonists artistically reflect the authors' views on truth, virtue, and beauty. In these novels, professional ethics is described as a high noble value. The scientific novelty of the research work includes the following: in the evolution of western and eastern poetic thought, in the context of the novel genre, the skill, common and distinctive aspects of the creation of an ideal hero were revealed by synthesis of effective methods in world science with literary criteria in the history of eastern and western literary studies, in the example of Sinclair Lewis and Pirimkul Kodirov.

Introduction and Preliminary Investigation of Buoyancy Assisted Robots That Are Cheap, Safe, and Will Never Fall Down

2021 ◽  
Author(s):  
Matthew David Williams ◽  
Dennis Hong
Keyword(s):  
Genetic Algorithm ◽  
Preliminary Analysis ◽  
Preliminary Investigation ◽  
Research Work ◽  
Legged Robots ◽  
Dynamical Analysis ◽  
Future Research ◽  
The Novel ◽  
Drag Forces ◽  
New Family

Abstract We introduce and define a new family of mobile robots called BAR (Buoyancy Assisted Robots) that are cheap, safe, and will never fall down. BARs utilize buoyancy from lighter-than-air gases as a way to support the weight of the robot for locomotion. A new BAR robot named BLAIR (Buoyant Legged Actuated Inverted Robot) whose buoyancy is greater than its weight is also presented in this paper. BLAIRs can walk “upside-down” on the ceiling, providing unique advantages that no other robot platforms can. Unlike other legged robots, the mechanics of how BARs walk is fundamentally different. We also perform a preliminary investigation for BARs. This includes comparing safety, cost, and energy consumption with other commercially available robots. Additionally, the preliminary investigation also includes analyzing previous works relating to BARs. A dynamical analysis is performed on the novel robot BLAIR. This is presented to show the impacts of buoyant and drag forces on BLAIRs. Preliminary analysis with the prevalence of drag is presented with simulations using a genetic algorithm and simulations. Results show that BARs with different mechanisms prefer different styles of walking gaits such as prancing or skipping. This work lays the foundation for future research work on the gaits for BARs.

scholarly journals An efficient DNA extraction method from bone and tooth samples by complete demineralization followed by the use of silica-based columns

2014 ◽  
Vol 23 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Md Mahamud Hasan ◽  
Tania Hossain ◽  
Ashish Kumar Majumder ◽  
Pilu Momtaz ◽  
Tarana Sharmin ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Extraction Method ◽  
Mechanical Grinding ◽  
High Concentration ◽  
Building Collapse ◽  
Dna Yield ◽  
Dna Extraction Method ◽  
Spin Column ◽  
Cryogenic Method ◽  
Dna Profiles

A highly efficient strategy for recovery of genomic DNA from bone and tooth samples is presented by complete demineralization of the bone pieces or intact tooth with high concentration of EDTA followed by spin column treatment without the need of mechanical grinding or cryogenic method for pulverizing the samples. The DNA yield was between 8 and 12 ng/?l from approximately 1 ?2 g of the starting material. Completed DNA profiles were obtained from of all the bones (52) and tooth (270) samples received from the unidentified victims from a recent building collapse, the Rana Plaza disaster in Dhaka, Bangladesh. DOI: http://dx.doi.org/10.3329/dujbs.v23i2.20089 Dhaka Univ. J. Biol. Sci. 23(2): 101-107, 2014

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