scholarly journals Exploration of the Characteristics of Intestinal Microbiota and Metabolomics in Different Rat Models of Mongolian Medicine

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Riao Dao ◽  
Dongxing Wu ◽  
Huan Wang ◽  
Habur Jin ◽  
Li Li ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Biosynthesis Pathway ◽  
Microbial Composition ◽  
Rat Models ◽  
Metabolism Pathway ◽  
Phenylpropanoid Biosynthesis ◽  
Underlying Mechanisms

Background. Mongolian medicine is a systematic theoretical system, which is based on the balance among Heyi, Xila, and Badagan. However, the underlying mechanisms remain unclear. This study aimed to explore the characteristics of intestinal microbiota and metabolites in different rat models of Mongolian medicine. Methods. After establishing rat models of Heyi, Xila, and Badagan, we integrated 16S rRNA gene sequencing and metabolomics. Results. Heyi, Xila, and Badagan rats had significantly altered intestinal microbial composition compared with rats in the MCK group. They showed 11, 18, and 8 significantly differential bacterial biomarkers and 22, 11, and 15 differential metabolites, respectively. The glucosinolate biosynthesis pathway was enriched only in Heyi rats; the biosynthesis of phenylpropanoids pathway and phenylpropanoid biosynthesis pathway were enriched only in Xila rats; the isoflavonoid biosynthesis pathway, the glycine, serine, and threonine metabolism pathway, and the arginine and proline metabolism pathway were enriched only in Badagan rats. Conclusions. The intestinal microbiota, metabolites, and metabolic pathways significantly differed among Heyi, Xila, and Badagan rats compared with control group rats.

scholarly journals Ratio of Klebsiella/Bifidobacterium in early life correlates with later development of paediatric allergy

2017 ◽  
Vol 8 (5) ◽  
pp. 681-695 ◽  
Author(s):  
J.S.Y. Low ◽  
S.-E. Soh ◽  
Y.K. Lee ◽  
K.Y.C. Kwek ◽  
J.D. Holbrook ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Allergic Disease ◽  
Early Life ◽  
16S Rrna Gene Sequencing ◽  
Early Infancy ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Healthy Infants ◽  
Increased Risk ◽  
Paediatric Allergy

Several studies have reported that intestinal microbial colonisation patterns differ between non-allergic and allergic infants. However, the microbial signature underlying the pathogenesis of allergies remains unclear. We aim to gain insight into the development of the intestinal microbiota of healthy infants and infants who develop allergy in early life, and identify potential microbiota biomarkers of later allergic disease. Using a case-control design in a Chinese sub-cohort of a Singaporean birth cohort (GUSTO), we utilised 16S rRNA gene sequencing to assess intestinal microbial composition and diversity of 21 allergic and 18 healthy infants at 3 weeks, 3 months and 6 months of age, and correlated the microbiota with allergy at ages 18 and 36 months. Pronounced differences in intestinal microbiota composition between allergic and healthy infants were observed at 3 months of age. The intestine of healthy infants was colonised with higher abundance of commensal Bifidobacterium. Conversely, Klebsiella, an opportunistic pathogen, was significantly enriched in the allergic infants. Interestingly, infants with a high Klebsiella/Bifidobacterium (K/B) ratio (above the population median K/B ratio) at age 3 months had an odds ratio of developing allergy by 3 years of age of 9.00 (95% confidence interval 1.46-55.50) compared to those with low K/B ratio. This study demonstrated a relationship between the ratio of genera Klebsiella and Bifidobacterium during early infancy and development of paediatric allergy in childhood. Our study postulates that an elevated K/B ratio in early infancy could be a potential indicator of an increased risk of allergy development. This line of research might enable future intervention strategies in early life to prevent or treat allergy. Our study provides new insights into microbial signatures associated with childhood allergy, in particular, suggests that an elevated K/B ratio could be a potential early-life microbiota biomarker of allergic disease.

scholarly journals The Effect of Xuebijing on the Gut Microbiota and Metabolic of Heat 1 Stroke Rat 2

2021 ◽  
Author(s):  
Qiang wen ◽  
Xuan He ◽  
Yu Shao ◽  
Lun Peng ◽  
Li Zhao ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gut Microbiome ◽  
Heat Stroke ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Metabolism Pathway ◽  
Rrna Gene Sequencing

Abstract The goal of the present study was to evaluate the fecal microbiome and serum metabolites in 16 Xuebijing (XBJ)-injected rats after heat stroke using 16S rRNA gene sequencing and gas chromatography-mass spectrometry (GC-MS) metabolomics. Eighteen rats were divided into the control group (CON), heat stroke group (HS), and XBJ group. The 16S rRNA gene sequencing results revealed that the abundance of Bacteroidetes was overrepresented in the XBJ group compared to the HS group, while Actinobacteria was underrepresented. Metabolomic profiling showed that the pyrimidine metabolism pathway, pentose phosphate pathway, and glycerophospholipid metabolism pathway were upregulated in the XBJ group compared to the HS group. Taken together, these results demonstrated that heat stroke not only altered the gut microbiome community structure of rats but also greatly affected metabolic functions, leading to gut microbiome toxicity.

scholarly journals Effect of Dietary Tryptophan on Growth, Intestinal Microbiota, and Intestinal Gene Expression in an Improved Triploid Crucian Carp

2021 ◽  
Vol 8 ◽  
Author(s):  
Yawei Fu ◽  
Xiaoxiao Liang ◽  
Donghua Li ◽  
Hu Gao ◽  
Yadong Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
16S Rrna ◽  
Intestinal Microbiota ◽  
Crucian Carp ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Ampk Signaling

Tryptophan (Trp) has received increasing attention in the maintenance of intestinal function. In this study, improved triploid crucian carp (ITCC) fed diets containing 6.35 g kg−1 Trp had higher average daily gain (ADG) and improved villus height (VH) and crypt depth (CD) in the intestine compared to the control group. To elucidate the potential mechanisms, we used RNA sequencing (RNA-seq) to investigate changes in the intestinal transcriptome and 16S rRNA gene sequencing to measure the intestinal microbiota in response to 6.35 g kg−1 Trp feeding in ITCC. Dietary Trp altered intestinal gene expression involved in nutrient transport and metabolism. Differentially expressed transcripts (DETs) were highly enriched in key pathways containing protein digestion and absorption and the AMPK signaling pathway. 16S rRNA sequencing showed that 6.35 g kg−1 Trp significantly increased the abundance of the genus Cetobacterium, and the Firmicutes/Bacteroidetes ratio at the phylum level (P < 0.05). In addition, bacterial richness indices (Simpson index) significantly increased (P < 0.05) community evenness in response to 6.35 g kg−1 Trp. In conclusion, appropriate dietary Trp improves the growth performance, and influences the intestinal flora of ITCC. This study might be helpful to guide the supply of dietary exogenous Trp in ITCC breeding.

scholarly journals The Impact of Cholecystectomy on the Gut Microbiota: A Case-Control Study

2019 ◽  
Vol 8 (1) ◽  
pp. 79 ◽  
Author(s):  
Won Yoon ◽  
Han-Na Kim ◽  
Eunkyo Park ◽  
Seungho Ryu ◽  
Yoosoo Chang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Enterohepatic Circulation ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Comprehensive Health ◽  
The Difference ◽  
Rrna Gene Sequencing ◽  
The Impact

Cholecystectomy alters the bile flow into the intestine and the enterohepatic circulation of the bile acids; this may affect the gut microbiota. We assessed the gut microbiota composition of patients who had undergone cholecystectomy and compared with those who had not. From a cohort of 1463 adult participants who underwent comprehensive health screening examinations, 27 subjects who had undergone cholecystectomy (cholecystectomy group) and 81 age- and sex-matched subjects who had not (control group) were selected. Clinical parameters were collected and compared. Microbial composition was determined by 16S rRNA gene sequencing of DNA extracted from fecal samples. We evaluated differences in the overall microbial composition and in the abundance of taxa. The two groups were comparable with respect to clinical characteristics and laboratory results. The actual number of taxa observed in a sample (observed features) was significantly lower in the cholecystectomy group than in the control group (p = 0.042). The beta diversity of Jaccard distance index was significantly different between the two groups (p = 0.027). Blautia obeum and Veillonella parvula were more abundant in the cholecystectomy group. The difference in the diversity of the gut microbiota between the cholecystectomy and control groups was subtle. However, B. obeum and V. parvula, which have azoreductase activity, were more abundant in the cholecystectomy group. The impact of such changes in the gut microbiota on health remains to be determined.

scholarly journals Combined Intestinal Metabolomics and Microbiota Analysis for Acute Endometritis Induced by Lipopolysaccharide in Mice

2021 ◽  
Vol 11 ◽  
Author(s):  
Yuqing Dong ◽  
Yuan Yuan ◽  
Yichuan Ma ◽  
Yuanyue Luo ◽  
Wenjing Zhou ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Bacterial Infections ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Chronic Infections ◽  
Intestinal Microbes ◽  
Specific Pathogen ◽  
Rrna Gene Sequencing ◽  
And Function

Endometritis is generally caused by bacterial infections, including both acute and chronic infections. In the past few decades, accumulated evidence showed that the occurrence of diseases might be related to gut microbiota. The progression of diseases is previously known to change the composition and diversity of intestinal microbiota. Additionally, it also causes corresponding changes in metabolites, primarily by affecting the physiological processes of microbiota. However, the effects of acute endometritis on intestinal microbiota and its metabolism remain unknown. Thus, the present study aimed to assess the effects of acute endometritis on intestinal microbes and their metabolites. Briefly, endometritis was induced in 30 specific pathogen-free (SPF) BALB/c female mice via intrauterine administration of lipopolysaccharide (LPS) after anesthesia. Following this, 16S rRNA gene sequencing and liquid chromatogram-mass spectrometry (LC-MS) were performed. At the genus level, the relative abundance of Klebsiella, Lachnoclostridium_5, and Citrobacter was found to be greater in the LPS group than in the control group. Importantly, the control group exhibited a higher ratio of Christensenellaceae_R−7_group and Parasutterella. Furthermore, intestinal metabolomics analysis in mice showed that acute endometritis altered the concentration of intestinal metabolites and affected biological oxidation, energy metabolism, and biosynthesis of primary bile acids. The correlation analysis between microbial diversity and metabolome provided a basis for a comprehensive understanding of the composition and function of the microbial community. Altogether, the findings of this study would be helpful in the prevention and treatment of acute endometritis in the future.

Difference of colorectal cancer microbial community by metagenomics and culture-based methods.

2019 ◽  
Vol 37 (4_suppl) ◽  
pp. 489-489
Author(s):  
Dong Ho Lee ◽  
Mijin Seol ◽  
Yu Ra Lee ◽  
Young Soo Park ◽  
Cheol Min Shin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Intestinal Microbiota ◽  
Agar Plate ◽  
Control Group ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Metagenome Analysis ◽  
The 16S Rrna Gene

489 Background: Dysbiosis of intestinal microbiota is promoting the development of colorectal cancer (CRC). We confirmed the intestinal microbiota composition from fecal sample of Korean CRC patients. Metagenomic analysis was performed and we isolated single microbes through culture-based method. Methods: CRC fecal samples were collected from 12 individuals. Metagenome Sequencing was based on the 16S rRNA gene amplicon on the Illumina MiSeq platform. The bacteria strains were subcultivated on the agar plate medium in aerobic and anaerobic and further identified by using the 16s rRNA gene sequencing. Results: Bacteria diversity by metagenome analysis was decreased in CRC group compared to control group. In CRC group, relative abundance of Firmicutes and Bacteroidetes were increased while the prevalence of Proteobacteria was decreased. The difference of microbial composition between control and CRC group was found at the genus level. Bacteroides, Parabacteroides of Bacteroidetes have increased and Acinetobacter, Pseudomonas of Proteobacteria have significantly decreased in CRC compared to control group. Using culture method, we isolated diverse bacteria of species level including five strains of Bacteroides; B. ovatus, B. uniformis, B. salyersiae, B. vulgatus and B. xylanisolvens and two strains of Fusobacterium; F. gonidiaformans and F. necrophorum from CRC patients. Conclusions: Metagenome analysis showed the genus Bacteroides, Parabacteroides of the phylum Bacteroidetes has increased and the genus Acinetobacter, Pseudomonas of Proteobacteria decreased in CRC group compared to control group. In addition to, we have isolated various strains associated with CRC by culture-based method.

scholarly journals The Effect of Dietary Fiber Modifications in Purified Diets Relative to Grain-Based Diets on Gastrointestinal Anatomy and Intestinal Microbial Communities in Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 685-685
Author(s):  
Elaine Glenny ◽  
Jintong Liu ◽  
Zorka Djukic ◽  
Michael Pellizzon ◽  
Ian Carroll
Keyword(s):  
Microbial Communities ◽  
Intestinal Microbiota ◽  
Undergraduate Research ◽  
16S Rrna Gene Sequencing ◽  
Published Data ◽  
Rrna Gene ◽  
Soluble Fiber ◽  
Microbial Composition ◽  
Additional Reference ◽  
The Impact

Abstract Objectives The use of compositionally defined purified diets (PDs)—diets with known sources and quantities of all nutrients—permits investigators to control this major environmental factor in rodent studies. However, mice fed a standard PD exhibit abnormal gastrointestinal (GI) anatomy compared to mice fed Purina 5001, a grain-based diet (GBD). Interestingly, the addition a soluble fiber (inulin) to PDs (typically only containing cellulose, an insoluble fiber) ameliorates these adverse effects. The impact of PDs on the intestinal microbiota has not yet been investigated. We therefore sought to identify PD-supplemented fiber(s) that best recapitulate the GI health and intestinal microbiota of mice fed a GBD, while also including an additional reference GBD (Teklad 2020SX). Methods 7-week-old C57BL/6J male mice were individually housed and randomly assigned to a diet (two GBDs and four PDs with varying fiber composition) for 28 days. To assess changes in GI anatomy, small intestinal and colon lengths and colon and cecal weights were recorded at tissue harvest. Cecal contents, colon contents, and fecal pellets were collected for 16S rRNA gene sequencing to compare microbial profiles across different GI niches and between diets using the Divisive Amplicon Denoising Algorithm (DADA2) pipeline. Results Consistent with published data, GI anatomy was altered in mice consuming PDs compared to the Purina GBD. However, there were no significant anatomical differences between mice consuming PDs and the Teklad GBD. Characterization of microbial communities revealed that the GI niche (cecum, colon, or feces) dictated microbial composition (P < 0.001, ANOSIM). Microbiotas from mice fed any PD significantly differed from mice consuming either GBD (P < 0.05, ANOSIM). Microbiotas were also distinct between mice fed either Purina 5001 or Teklad 2020SX (P < 0.01, ANOSIM). Conclusions These data suggest that Purina 5001 does not represent all GBDs and that PDs may not significantly alter rodent GI anatomy compared to GBDs. As each diet tested significantly altered the microbial community, future work will seek to determine whether a specific PD-associated gut microbiota is beneficial to GI health. Funding Sources The NIH, the Honors Carolina Sarah Steele Danhoff Undergraduate Research Fund, and Research Diets, Inc.

scholarly journals Dynamic Observation of the Effect of Maternal Caries on the Oral Microbiota of Infants Aged 12–24 Months

2021 ◽  
Vol 11 ◽  
Author(s):  
Fei Li ◽  
Ding Fu ◽  
Danying Tao ◽  
Xiping Feng ◽  
May Chun Mei Wong ◽  
...  
Keyword(s):  
Dental Caries ◽  
16S Rrna Gene Sequencing ◽  
Control Group ◽  
Rrna Gene ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Mothers And Children ◽  
Group A ◽  
Rrna Gene Sequencing ◽  
Dynamic Description

AimTo provide a dynamic description of the oral microbial composition in mothers with and without dental caries and their children aging 12-24 months.MethodologyA total of 20 pairs of mothers and their children aged 12 months were included and followed up at 18 and 24 months of age. Ten mothers with dental caries(MEG) and their children(CEG) were in the exposure group, and ten caries-free mothers(MCG) and their children(CCG)in control group. Supragingival plaque biofilm samples were collected and DNA was extracted for bacterial 16S rRNA gene sequencing.ResultsA total of 18 pairs completed follow-ups. At a 3% divergence level, the number of common operational taxonomic units found between the mothers and children increased as the children aged. Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria accounted for more than 80% phyla of each group. A microbial community structure analysis showed that the differences between mothers and children were significant in all groups except for the MEG24 and CEG24 groups.ConclusionsOral microbiota of children was more like their mothers’ with increasing age, regardless of whether the mothers had dental caries. Mothers with dental caries may have a greater influence on the oral microbiota of children’s than those without dental caries as children age.

scholarly journals Necrotizing Enterocolitis and Intestinal Microbiota: The Timing of Disease and Combined Effects of Multiple Species

2021 ◽  
Vol 9 ◽  
Author(s):  
Xin Fu ◽  
Siwen Li ◽  
Yanfang Jiang ◽  
Xintong Hu ◽  
Hui Wu
Keyword(s):  
Necrotizing Enterocolitis ◽  
Intestinal Microbiota ◽  
Premature Infants ◽  
Metabolic Pathways ◽  
Microbial Colonization ◽  
Control Group ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Linear Discriminant ◽  
Multiple Species

Background: The purpose of this study was to investigate the relationship between intestinal microbiota and necrotizing enterocolitis (NEC).Methods: 16S rRNA gene sequencing was used to compare the microbial composition of feces. The first sample was collected within 48 h after birth, then once per week until the NEC diagnosis, and finally 1–2 weeks after treatment or 28 days after birth.Results: The alpha diversity of the microbiota in the NEC group was higher than that in the control group. Beta diversity analysis showed that the control group had a higher similarity at the onset of NEC, while the NEC group was distributed in subgroups. Linear discriminant analysis effect size and taxonomic composition analyses indicated that the abundance of Bacteroides and Actinobacteria in NEC infants at birth was much higher than that in the control group, and this trend continued until NEC occurred. At this time, Rhizobiales, Dysgonomonas, Ochrobactrum, Ralstonia, Pelomonas, Acinetobacter, etc., were also more abundant in NEC infants. The upregulated different metabolic pathways in the NEC group were mainly concentrated on degradation/utilization/assimilation, biosynthesis, and generation of precursor metabolites and energy.Conclusions:1. The microbial community differs according to the time of NEC diagnosis (bounded by 20 days).2. No single microorganism is related to NEC, and the combined effect of multiple species is of great significance in the occurrence of NEC. Premature infants are easily affected by bacteria living in the environment, and compared with ordinary premature infants, NEC infants have a higher abundance of waterborne bacteria. Therefore, attention should be paid to the contamination of water sources and various ventilator pipelines for premature infants hospitalized in the neonatal intensive care unit.3. An in-depth study of the mode of microbial colonization in premature infants combined with the different functions of various metabolic pathways involved in different microorganisms may be able to identify the cause of NEC.

scholarly journals Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 254
Author(s):  
Ying Wang ◽  
Jianqing Zhu ◽  
Jie Fang ◽  
Li Shen ◽  
Shuojia Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Adult Survival ◽  
Rrna Gene ◽  
Life Stages ◽  
Microbial Composition ◽  
Significant Difference ◽  
Functional Inference ◽  
Rrna Gene Sequencing ◽  
Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

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