scholarly journals Anti-Inflammatory Activity and Quality Control of Erysimum cheiri (L.) Crantz

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ghazaleh Mosleh ◽  
Amir Azadi ◽  
Sedigheh Khademian ◽  
Reza Heidari ◽  
Abdolali Mohagheghzadeh
Keyword(s):  
Quality Control ◽  
Diclofenac Sodium ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Drug Candidate ◽  
Aerial Parts ◽  
Stretching Vibration ◽  
Ft Ir ◽  
Anti Inflammatory

Wallflower (Erysimum cheiri) is employed as a popular herbal drug in traditional Persian medicine. Topical formulations including cerates, lotions, sitz baths, and poultices for inflammatory disorders such as arthritis, anal fissure, endometriosis, and mastitis are known. However, there is no monograph in current pharmacopoeia for the wallflower drug. The present study is aimed to screen in vitro anti-inflammatory activity of wallflower and perform quality control and characterization tests for different organs of the herb. In this regard, albumin denaturation activity, macroscopic and microscopic, phytochemical, HPTLC, and FT-IR characteristics were investigated. Wallflower showed strong anti-inflammatory activity compared to diclofenac sodium. The root (1.25, 2.5, and 5 mg/mL) and flower (10 mg/mL) extract exhibited higher anti-inflammatory activities than that of other plant organs at the same concentrations. Moreover, total ash was found higher in aerial parts ( 21.52 ± 0.06 % ) than flower ( 11.01 ± 0.03 % ), root ( 5.03 ± 0.03 % ), and seed ( 6.95 ± 0.06 % ), while water-soluble ash was higher in seed ( 34.89 ± 0.26 % ) than flower ( 5.00 ± 0.03 % ), aerial parts ( 7.16 ± 0.06 % ), and root ( 5.04 ± 0.01 % ). Acid-insoluble ash and sulphated ash were higher in root ( 9.50 ± 0.04 % ) and aerial part ( 28.37 ± 0.57 % ), respectively. In addition, loss on drying was ranged from 2.20 ± 0.20 % in flowers to 6.00 ± 0.10 % in aerial parts. On the other hand, HPTLC analysis verified cardenolide compounds in all organs of the herb, and quercetin was detected in the flavonoid fingerprint of acid hydrolysed flowers. According to FT-IR results, the observed spectral region at ~3500 cm-1 attributed to -OH stretching vibration. Also, C–H (~2900-2950 cm-1), isothiocyanate (~2340 cm-1), -C=O (~1740 cm-1), conjugated C=C of the aromatic ring (~1650 cm-1), and structure of the aromatic group (~1200-1000 cm-1) were monitored. This work is the first study to the best of our knowledge, suggesting wallflower as a potential drug candidate with the basis for a monograph in addition to initial in vitro anti-inflammatory data.

scholarly journals ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

2017 ◽  
Vol 10 (6) ◽  
pp. 160
Author(s):  
Vinueza D ◽  
LÓpez E ◽  
Acosta K ◽  
Abdo S
Keyword(s):  
Freeze Drying ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Hydroalcoholic Extract ◽  
Cytotoxicity Assays ◽  
Aerial Parts ◽  
Controlled Conditions ◽  
Freeze Dried ◽  
Anti Inflammatory

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatoryactivity compared to aspirin, with inflammatory inhibition percent values of 80.138±0.729 to hydroalcoholic extract of B. andicola and 82.117±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference. 

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

2017 ◽  
Vol 9 (6) ◽  
pp. 145
Author(s):  
Thriveni Vasanth Kumar ◽  
Manjunatha H. ◽  
Rajesh Kp
Keyword(s):  
Acetic Acid ◽  
Protective Effect ◽  
Vascular Permeability ◽  
Diclofenac Sodium ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
The Individual

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

scholarly journals Synthesis, Characterization and Evaluation of Anti-inflammatory Activity of Novel Indoline Derivatives

2016 ◽  
Vol 12 (12) ◽  
pp. 4557-4563
Author(s):  
Dhinesh kumar Manoharan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Potent Inhibition ◽  
Ft Ir ◽  
Anti Inflammatory

Series of indoline derivatives were synthesized using N-(4-aminophenyl) indoline-1-carbothiamide as a precursor. The structures of synthesized compounds were confirmed by   FT-IR, 1H-NMR, 13C-NMR and LC-MS. The in vitro anti-inflammatory activity of synthesized indoline derivatives were examined by standard anti-denaturation assay. The compounds 4a (IC50 = 62.2 µg/ml) and 4b (IC50 = 60.7 µg/ml) showed potent inhibition on protein denaturation. The compounds 5a (IC50 = 97.8 µg/ml) exhibits moderate inhibition on protein denaturation

Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

2021 ◽  
pp. 169-173
Author(s):  
K.P. Jaiganesh ◽  
T.J. Jasna ◽  
A.C. Tangavelou
Keyword(s):  
Tamil Nadu ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Traditional System ◽  
Antimicrobial Potential ◽  
Biochemical Compounds ◽  
Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

IN VITRO ANTIARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF PETETHER EXTRACT OF HYPTIS SUAVEOLENS

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (09) ◽  
pp. 60-62
Author(s):  
M. R Elayaraja Krishnan ◽  
◽  
R. Vijaya Kanth ◽  
S. Muthu Pandi
Keyword(s):  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
In Vitro Methods ◽  
Whole Plant ◽  
Hyptis Suaveolens ◽  
Excellent Activity ◽  
Anti Inflammatory ◽  
The Stability

In our present study, various concentrations of pet ether extract of whole plant of Hyptis suaveolens were prepared and subjected for anti arthritic and anti-inflammatory activity by in vitro methods. The antiarthritic activity was carried out by bovine serum albumin denaturation paradigm. The results showed that the pet ether extract (44.51%) showed excellent arthritic activity when compared to the standard diclofenac sodium (31.44%) at 2000μg/mL. Meanwhile, in the anti-inflammatory paradigm, the same extract (99.10%) showed excellent activity than the standard diclofenac sodium (73.80%) at 2000μg/ mL by restoring the stability of degraded red blood corpuscles. In both the paradigms, increase in concentration of the extract increases the action potential against the methanol degradation of bovine albumin and HRBC.

scholarly journals Formulation and Evaluation of Liposome by Thin Film Hydration Method

2021 ◽  
Vol 11 (1) ◽  
pp. 72-76
Author(s):  
Mahesh Umbarkar ◽  
Swapnil Thakare ◽  
Tanaji Surushe ◽  
Amol Giri ◽  
Vaibhav Chopade
Keyword(s):  
Thin Film ◽  
Diclofenac Sodium ◽  
Entrapment Efficiency ◽  
Repeated Administration ◽  
Liposomal Formulation ◽  
Inflammatory Activity ◽  
In Vitro Drug Release ◽  
Controlled Drug Delivery System ◽  
Anti Inflammatory

Liposomes are the most advance formulation for targeting and controlled drug delivery system. These liposomes are generally administered by intra-venous route. In this work the liposome was prepared by using thin film hydration method. The formulated liposome is evaluated or characterised by using zeta sizer, Encapsulation efficiency, Entrapment efficiency, In vitro drug release. Main things are drug which are used for formulation of liposome was Diclofenac sodium, it having anti-inflammatory and anti-pyretic effect. The Diclofenac sodium having several adverse effects, such as depression of renal function, Liver failure for repeated administration, Local mucosal irritation, gastritis. To avoid this adverse effect Diclofenac sodium are incorporate in liposomal formulation. By formulating liposomal formulation, the bioavailability of Diclofenac sodium increase. In conventional dosage form bioavailability of diclofenac sodium is 50℅. But in liposomal formulation bioavailability of this drug increase. The final result includes that diclofenac liposome formulation shows more sustained and prolong anti-inflammatory activity. Keywords: Diclofenac sodium, Liposome, Anti-inflammatory activity.

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT AND DIFFERENT FRACTIONS OF CENTELLA ASIATICA LEAVES BY PROTEIN DENATURATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 86-89
Author(s):  
S Sharma ◽  
◽  
R. Trivedi ◽  
N. K. Choudhary
Keyword(s):  
Petroleum Ether ◽  
Skin Diseases ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation might be a complex organic reaction to a hazardous stimulant such as pathogens, or injured tissues and mainly causes itching, swelling, skin redness, warm and slight pain. Herbal drugs are widespread in India for their effectiveness, easy availability at low cost and provide low toxicity as compared to modern drugs. Centella asiatica is one of the oldest Ayurvedic medicinal plants, used in treatment of various skin diseases. The aim of our present research was to evaluate the in vitro anti-inflammatory activity of methanolic extract and different fractions of C. asiatica leaves. In protein denaturation method, the percentage inhibition for methanolic extract was observed to be 40.22%. The petroleum ether and n-butanol fraction of methanolic extract of C. asiatica were observed to exhibits 54.12 and 44.42% inhibition, respectively. Diclofenac sodium was used as a standard drug. In comparison with other fractions petroleum ether and n-butanol fractions showed best activity. The preliminary phytochemical studies of n-butanol fractions and n-butanol fractions showed the presence of terpenoids, flavonoids etc., which are used in the treatment of inflammation. Thus, we can call the latter as intense anti-inflammatory agent.

scholarly journals Assessment of In vitro Anti-inflammatory Activity of Ginger and Diclofenac sodium combination

2020 ◽  
pp. 442-447
Author(s):  
Mousmi D. Thakur ◽  
Navin R. Sheth ◽  
Mihir K. Raval
Keyword(s):  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Research Work ◽  
Pharmacological Action ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Ginger Extract ◽  
Anti Inflammatory

The present research work aimed at evaluating the anti-inflammatory activity of Zingiber officinalis with Diclofenac sodium by HRBC membrane stabilization & Protein denaturation. The precluding of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization at different concentrations was performed for methanolic, hydro-methanolic ginger extract and diclofenac sodium. At a dose of 50µg/ml the maximum membrane stabilization 86.34% was found for Ginger extract(test) and at a dose of 500 mcg/ml membrane stabilization was found 91.16% for diclofenac sodium(standard) and the membrane stabilization for combination (ginger with diclofenac sodium) at a dose of 50µg/ml was recorded 86.43%, as the concentration increase(1000 mcg/ml) for combination(ginger with diclofenac sodium) the percentage protection was decreased. In vitro protein denaturation was performed by using egg albumin method. Maximum inhibition was observed in case of methanolic extract of ginger at concentration 1000mcg/ml and it was 78.83±5.17 and in hydro methanolic extract for Diclofenac sodium at concentration 1000mcg/ml and it was 63.37±2.78.Minimum inhibition observed in combination of methanolic extract of ginger and diclofenac sodium at concentration 1000mcg/ml and it was 25.27±1.76 and in combination of hydro-methanolic extract of ginger and Diclofenac sodium at concentration 1000mcg/ml and it was 28.23±3.14. The results of this study divulge that low dose combination of ginger and diclofenac sodium has higher anti-inflammatory activity than diclofenac sodium and ginger alone. With this initial study, research work could be extended further; therefore, the particular pharmacological action for the combination of ginger with diclofenac sodium could be discovered.

Phytochemical, Quantitative Proximate and In vitro Anti-inflammatory Study of the Crude Methanol Extract of Stachytarpheta indica Leaves (Verbenaceae)

2019 ◽  
pp. 153-162
Author(s):  
Cyril Ogbiko ◽  
Dickson Achimugu Musa ◽  
Musa Usman Dabai ◽  
Ibeabuchi Jude Ali ◽  
Abubakar Sani Yelwa ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Methanol Extract ◽  
Proximate Analysis ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Crude Methanol Extract

Herbal medicine despite its increase usage globally is being plagued by inadequate research on their effectiveness. Stachytarpheta indica commonly known as snakeweed is used locally in the management of asthma, headache, alopecia, bronchitis, bruise, chest cold, constipation, itch, diarrhea, skin sore, vermifuge, dysentery, dysmenorrhea among others. This study investigated the phytochemical composition, quantitative proximate parameters and in vitro anti-inflammatory activity of the leaves of S. indica using established methods. Results confirmed the presence of alkaloids, saponins, carbohydrate, cardiac glycosides, terpenoid, tannin, anthraquinones, phenolics and flavonoid. Moisture content (9.16 ± 0.04%), total ash (19.42 ± 0.15%), water insoluble ash (7.17 ± 0.22%), acid insoluble ash (3.63 ± 0.26%), alcohol soluble extractive (0.99 ± 0.06%) and water-soluble extractive (0.36 ± 0.02%) values were obtained from the quantitative proximate analysis. An infusion of the methanol leaf extract of Stachytarpheta indica demonstrated significant anti-inflammatory activity in a dose dependent manner comparable to that of indomethacin standard.

Sign in / Sign up

Export Citation Format

Share Document