scholarly journals Fabrication and Characterization of Chitosan-Polyethylene Glycol (Ch-Peg) Based Hydrogels and Evaluation of Their Potency in Rat Skin Wound Model

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Md Mahmudul Hasan ◽  
Md Forhad Uddin ◽  
Nayera Zabin ◽  
Md Salman Shakil ◽  
Morshed Alam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyethylene Glycol ◽  
3D Structure ◽  
Bound Water ◽  
Skin Wound ◽  
Rat Skin ◽  
Composite Hydrogels ◽  
Wound Model ◽  
Polymeric Networks ◽  
Hplc Grade Water

Thermal burns are a major cause of death and suffering around the globe. They can cause debilitating, life-altering injuries as well as lead to significant psychological and financial consequences. Several research works have been conducted in attempt to find a wound healing therapy that is successful. At present, hydrogels have been widely used in cutting-edge research for this purpose because they have suitable properties. This study aimed to see how therapy with chitosan-polyethylene glycol (Ch-Peg) based hydrogels affected the healing of burn wounds in rats. With the concern of public health, xanthan gum (X), boric acid (B), gelatin (Ge), polyethylene glycol (Peg), chitosan (Ch), glutaraldehyde (G), and HPLC-grade water were prepared using X : Ge : G, X : Ge : Peg : G, X : Ge : Ch : G, X : Ge : Peg : Ch : G, X : Ge : B : Ch : G, X : Ge : B : Peg : G, and X : Ge : B : Peg : Ch : G. The produced composite hydrogels were examined for swelling ability, biodegradability, rheological characteristics, and porosity. The 3D structure of the hydrogel was revealed by scanning electron microscopy (SEM). After that, the structural characterization technique named Fourier-transform infrared spectroscopy (FTIR) was used to describe the composites (SEM). Lastly, in a rat skin wound model, the efficacy of the produced hydrogels was studied. Swelling ability, biodegradability, rheological properties, and porosity were all demonstrated in composite hydrogels that contained over 90% water. Hydrogels with good polymeric networks and porosity were observed using SEM. The existence of bound water and free, intra- and intermolecule hydrogen-linked OH and NH in the hydrogels was confirmed using FTIR. In a secondary burned rat model, all hydrogels showed significant wound healing effectiveness when compared to controls. When compared to other composite hydrogels, wounds treated with X : Ge : Peg : Ch : G, X : Ge : B : Peg : G, and X : Ge : B : Peg : Ch:G recovered faster after 28 days. In conclusion, this research suggests that X : Ge : Peg : Ch : G, X : Ge : B : Peg : G, and X : Ge : B : Peg : Ch : G could be used to treat skin injuries in the clinic.

scholarly journals Fabrication of Xanthan gum: Gelatin (Xnt:Gel) Hybrid Composite Hydrogels for Evaluating Skin Wound Healing Efficacy

2019 ◽  
Vol 13 (3) ◽  
pp. 101 ◽  
Author(s):  
Mohammad Mahfuz Ali Khan Shawan ◽  
Nazmul Islam ◽  
Shahin Aziz ◽  
Nazia Khatun ◽  
Satya Ranjan Sarker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Xanthan Gum ◽  
Physicochemical Characterization ◽  
Bound Water ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Composite Hydrogels ◽  
Ir Studies ◽  
Wound Model ◽  
Polymeric Networks

With the background of snowballing threat of skin wound to public health and economy, this study was undertaken utilizing xanthan gum (Xnt), citric acid (C), gelatin (Gel), glutaraldehyde (G) and HPLC-grade water to fabricate a series of composite hydrogels i.e. Xnt, Xnt:C, Xnt:Gel(3):G, Xnt:C:Gel(3):G, Xnt:Gel(5):G, Xnt:C:Gel(5):G for investigating their wound healing efficacy in experimental rat skin wound model. Physicochemical characterization revealed that all the composite hydrogels contained more than 90% water. The hydrogels displayed swelling ability, biodegradability, good polymeric networks and porosity. Fourier Transform Infrared Spectroscopy (FT-IR) studies confirmed the presence of bound water and free, intra and inter molecular bound hydrogen bonded OH and NH in the hydrogels. All the hydrogels showed significant wound healing potency in experimental deep second degree skin burns in rats compared to controls. 20 days post-application of hydrogels, Xnt:Gel(3):G, Xnt:Gel(5):G and Xnt:C:Gel(5):G-treated wounds showed better recovery compared to other composite hydrogels. We conclude that, Xnt:Gel(3):G, Xnt:Gel(5):G and Xnt:C:Gel(5):G might be effective wound dressing material.

Study of the wound healing effects of a gun oil sample on a rat skin wound model

2020 ◽  
Vol 69 (8) ◽  
pp. 43-49
Author(s):  
Ekaterina Igorevna Trofmets ◽  
Yaroslav Aleksandrovich Gushchin ◽  
Kirill Leonidovich Kryshen ◽  
M.N. Makarova ◽  
Sergei Mikhailovich Mamykin
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Rat Skin ◽  
Wound Model

In Vivo and ex Vivo Approaches to Studying the Biomechanical Properties of Healing Wounds in Rat Skin

2013 ◽  
Vol 135 (10) ◽  
Author(s):  
Clare Y. L. Chao ◽  
Gabriel Y. F. Ng ◽  
Kwok-Kuen Cheung ◽  
Yong-Ping Zheng ◽  
Li-Ke Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ex Vivo ◽  
Normal Skin ◽  
Biomechanical Properties ◽  
Skin Wound ◽  
Sprague Dawley ◽  
Rat Skin ◽  
Mechanical Stiffness ◽  
Air Jet

An evaluation of wound mechanics is crucial in reflecting the wound healing status. The present study examined the biomechanical properties of healing rat skin wounds in vivo and ex vivo. Thirty male Sprague-Dawley rats, each with a 6 mm full-thickness circular punch biopsied wound at both posterior hind limbs were used. The mechanical stiffness at both the central and margins of the wound was measured repeatedly in five rats over the same wound sites to monitor the longitudinal changes over time of before wounding, and on days 0, 3, 7, 10, 14, and 21 after wounding in vivo by using an optical coherence tomography-based air-jet indentation system. Five rats were euthanized at each time point, and the biomechanical properties of the wound tissues were assessed ex vivo using a tensiometer. At the central wound bed region, the stiffness measured by the air-jet system increased significantly from day 0 (17.2%), peaked at day 7 (208.3%), and then decreased progressively until day 21 (40.2%) as compared with baseline prewounding status. The biomechanical parameters of the skin wound samples measured by the tensiometer showed a marked reduction upon wounding, then increased with time (all p < 0.05). On day 21, the ultimate tensile strength of the skin wound tissue approached 50% of the normal skin; while the stiffness of tissue recovered at a faster rate, reaching 97% of its prewounded state. Our results suggested that it took less time for healing wound tissues to recover their stiffness than their maximal strength in rat skin. The stiffness of wound tissues measured by air-jet could be an indicator for monitoring wound healing and contraction.

scholarly journals Adenosine diphosphate contributes to wound healing in diabetic mice through P2Y1 and P2Y12 receptors activation

2020 ◽  
Author(s):  
Paula A. Borges ◽  
Ingrid Waclawiak ◽  
Janaína L. Georgii ◽  
Janaína F. Barros ◽  
Vanderlei S. Fraga-Junior ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Diseases ◽  
Purinergic Signaling ◽  
Adenosine Diphosphate ◽  
Treg Cells ◽  
Skin Wound ◽  
Diabetic Patients ◽  
Dependent Manner ◽  
Keratinocyte Proliferation ◽  
Wound Model

AbstractSeveral studies have shown the importance of purinergic signaling in various inflammatory diseases. In diabetes mellitus, there is an increase in the activity of some nucleotidases suggesting that this signaling may be affected in the diabetic skin. Thus, the aim of our study was to investigate the effect of ADP on wound healing in diabetic skin. Swis and C57BL/6 mice were pharmacologic induced to type 1 diabetes and submitted to a full-thickness excisional wound model to evaluate the effect of ADP as a topic treatment. Adenosine diphosphate accelerated cutaneous wound healing, improved the new tissue formation, and increased collagen deposit by positively modulating P2Y1 and P2Y12 and TGF-β production. In parallel, ADP reduced reactive oxygen species production and TNF-α levels, while increased IFNγ, IL-10 and IL-13 levels in the skin. Also, ADP induced the migration of neutrophils, eosinophils, mast cells, TCRγ4+, and TCRγ5+ cells while reduced Treg cells towards the lesion at day 7. In accordance, ADP increased the proliferation and migration of fibroblast, induced myofibroblast differentiation and keratinocyte proliferation in a P2Y12-dependent manner. We provide the first evidence of ADP acting as a potent mediator on skin wound resolution and a possible therapeutic approach for diabetic patients worldwide.

scholarly journals Ischemic Non-Healing Skin Wound Model: Cell Death and Wound Healing Mechanisms

2018 ◽  
Vol 10 (4) ◽  
pp. 69
Author(s):  
E.I. Morgun ◽  
O.S. Rogovaya ◽  
E.A. Vorotelyak
Keyword(s):  
Wound Healing ◽  
Cell Death ◽  
Skin Wound ◽  
Wound Model ◽  
Model Cell

Fabrication of Xanthan Gum: Gelatin (Xnt:Gel) Hybrid Composite Hydrogels for Investigating Skin Wound Healing Efficacy

2021 ◽  
pp. 130-143
Author(s):  
Mohammad Mahfuz Ali Khan Shawan ◽  
Nazmul Islam ◽  
Shahin Aziz ◽  
Nazia Khatun ◽  
Satya Ranjan Sarker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hybrid Composite ◽  
Xanthan Gum ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Composite Hydrogels

scholarly journals Amnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model

2019 ◽  
Vol 9 (1) ◽  
pp. 80-92 ◽  
Author(s):  
Sean V. Murphy ◽  
Aleksander Skardal ◽  
Ronald A. Nelson ◽  
Khiry Sunnon ◽  
Tanya Reid ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Full Thickness ◽  
Porcine Skin ◽  
Accelerate Wound Healing ◽  
Wound Model ◽  
Amnion Membrane

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin&nbsp;14, &alpha;-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

scholarly journals An mRNA sequencing analysis of the healing-promoting role of electroacupuncture a in rat skin wound model

2020 ◽  
Vol 9 (4) ◽  
pp. 1462-1475
Author(s):  
Weibin Du ◽  
Guanai Bao ◽  
Huahui Hu ◽  
Rongliang Chen ◽  
Fuxiang Shen ◽  
...  
Keyword(s):  
Skin Wound ◽  
Sequencing Analysis ◽  
Rat Skin ◽  
Mrna Sequencing ◽  
Wound Model

Tofu-Based Hybrid Hydrogels with Antioxidant and Low Immunogenicity Activity for Enhanced Wound Healing

2019 ◽  
Vol 15 (7) ◽  
pp. 1371-1383 ◽  
Author(s):  
Jun Huang ◽  
Long Chen ◽  
Qijuan Yuan ◽  
Zhipeng Gu ◽  
Jun Wu
Keyword(s):  
Wound Healing ◽  
Free Radicals ◽  
Antioxidant Activities ◽  
Skin Wound ◽  
Hybrid Hydrogel ◽  
Wound Model ◽  
Hybrid Hydrogels ◽  
Thickness Skin ◽  
Regeneration Effect

Free radicals and inflammation in the skin suffering from trauma cause oxidative damage and delayed healing, leading to adverse wound conditions. To adequately investigate the effects of free radicals and controlled immunogenicity for wound healing, we propose a tofu-based hybrid hydrogel with antioxidant and low immunogenicity properties that can be used for wound healing. Tofu, a food source material, was introduced for the first time into gelatin methacryloyl (GelMA) hydrogels by the photo-crosslinking method. The results demonstrated that the incorporation of tofu influenced the pores, swelling, water vapor transmission and compressive properties of hydrogels greatly. The antioxidant activities of hydrogels had been enhanced with increasing rations of tofu, and the fibroblast culture showed good proliferation on the hybrid hydrogels, as well as slight immunogenicity, thereby inducing the M2 differentiation of macrophages. Further, a full-thickness skin wound model was created to study the healing effect of hybrid hydrogels. In vivo results confirmed that the antioxidant activity and slight immunological stimulation properties of tofu hydrogels could accelerate the wound healing rate and improve the skin tissue regeneration effect. The present study validates that the tofu-based hybrid hydrogels have multiple bioactivities and could be potential antioxidant and immunoregulation hydrogels in wound healing applications.

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