scholarly journals Histological Analysis of Bone Callus in Delayed Union Model Fracture Healing Stimulated with Pulsed Electromagnetic Fields (PEMF)

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
U. Umiatin ◽  
Ismail Hadisoebroto Dilogo ◽  
Puji Sari ◽  
Sastra Kusuma Wijaya
Keyword(s):  
Fracture Healing ◽  
Histological Analysis ◽  
Healing Process ◽  
Fibrous Tissue ◽  
Delayed Union ◽  
Control Group ◽  
Left Femur ◽  
Physical Methods ◽  
Bone Callus ◽  
Pulsed Electromagnetic

Delayed union and nonunion fractures are clinical challenges for orthopedic surgeons. The development of fracture complications, such as delayed union and nonunion fractures, is still difficult to predict. Various methods are being investigated to improve fracture healing and prevent complications in patients. There are various methods to promote fracture healing, broadly divided into biological, chemical, and physical methods. One of the most widely used physical methods to promote fracture healing is the pulsed electromagnetic field (PEMF). This study aimed to evaluate the healing process of delayed union fracture after being stimulated by PEMF. Twenty-four rats were randomly divided into two groups: the control group (n = 12) and the PEMF group (n = 12). Delayed union fracture was performed on the left femur of all rats. Subsequently, the PEMF group was given PEMF stimulus with a magnetic field intensity of 1.6 mT and a frequency of 50 Hz for 4 hours/day and 7 days/week. The fracture healing process was evaluated on days 5, 10, 18, and 28 based on the bone callus histology using safranin O fast green (SOFG) staining. The results of the histological analysis showed that bone cartilage was higher in the PEMF group than in the control group throughout the observation period. In addition, the PEMF group had less fibrous tissue at the beginning of the healing. This finding indicates PEMF stimulation has an effect on inducing osteogenesis on fracture healing and reducing the risk of delayed union.

scholarly journals EXTREMELY LOW-FREQUENCY-PULSED ELECTROMAGNETIC FIELD EXPOSURE IN THE HEALING PROCESS OF SPRAGUE-DAWLEY RATS WITH DELAYED-UNION FEMUR FRACTURE: A STUDY OF THE FAILURE LOAD OF AXIAL FORCE

2019 ◽  
pp. 17-19
Author(s):  
ANDIKA D. DJAJA ◽  
ERWIN A. NOOR ◽  
ISMAIL H. DILOGO
Keyword(s):  
Electromagnetic Field ◽  
Fracture Healing ◽  
Failure Load ◽  
Healing Process ◽  
Low Frequency ◽  
Delayed Union ◽  
Pulsed Electromagnetic Field ◽  
Extremely Low Frequency ◽  
Significant Difference ◽  
Pulsed Electromagnetic

Objective: Under normal conditions, fractures can heal, but under some conditions, complications can occur, such as delayed union or nonunion. Interaction between the processes of angiogenesis and osteogenesis (the interaction of osteoblast and osteoclast) is the determining factor in the healing process. Exposure to an electromagnetic field, as a physical stimulus, affects osteogenesis both in the developmental stage of the embryo and in the fracture healing process. This study was conducted to determine the healing of delayed-union fractures through exposure to an extremely low-frequency-pulsed electromagnetic field (ELF-PEMF), comparing the failure load scores in experimental animals. Methods: The study was conducted in the Faculty of Medicine, Universitas Indonesia, with 56 experimental rats during August and September 2018. Results: There was a significant difference in the failure load score in both groups in the fourth and fifth weeks of the study. There were no differences in clinical improvement in the two groups. Conclusion: This study concluded that there was an improvement in delayed-union fracture healing after the administration of ELF-PEMF, as seen in the difference in failure load scores.

scholarly journals The effect of Extreme Low Frequency-Pulsed Electromagnetic Field exposure in the healing process of Sprague Dawley mouse delayed union femur fracture: study of Rust Radiology Score

2020 ◽  
Vol 4 (1) ◽  
pp. 10
Author(s):  
Andika Djaja
Keyword(s):  
Electromagnetic Field ◽  
Fracture Healing ◽  
Healing Process ◽  
Low Frequency ◽  
Development Stage ◽  
Delayed Union ◽  
Sprague Dawley ◽  
Pulsed Electromagnetic Field ◽  
Field Exposure ◽  
Pulsed Electromagnetic

Fractures usually heal normally. In some conditions, the healing process do not occur normally, but become delayed union or non union as a complication. Successful healing of fractures is a complex interaction between the process of angiogenesis and osteogenesis (the interaction of osteoblasts and osteoclast). Physical stimuli such as exposure of EMF (electromagnetic fields) influences of the osteogenesis process both in the development stage of embryo reinforcement and the fracture healing stage. The aim of this study is to determine the healing of delayed union fractures in experimental animals due to the expossure of Extreme Low Frequency-Pulsed Electromagnetic Field (ELF-EMF) by comparing the RUST scores. The experimental study was conducted Department of Nutrition Animal Laboratory, Faculty of Medicine, Universitas Indonesia with 56 experimental rats during August-September 2018. There were no differences of animal characteristics in the study. It was found that there were significant differences in Rust Score in the treatment and control groups in each examination week. There were no differences in clinical improvement in the two groups.  This study concluded that there was an improvement in delayed union fracture healing after the administration of ELF-EMF as seen from the difference in Rust score.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals The Effect of Strontium Ranelate on Fracture Healing: An Animal Study

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ourania I. Koukou ◽  
Lampros D. Pappas ◽  
Pelagia Chloropoulou ◽  
Maria A. Kouroupi ◽  
Konstantinos I. Koukos ◽  
...  
Keyword(s):  
Treatment Group ◽  
Fracture Healing ◽  
Strontium Ranelate ◽  
Fibrous Tissue ◽  
Rabbit Model ◽  
Control Group ◽  
Biomechanical Assessment ◽  
Osteoclast Number ◽  
Histopathological Studies ◽  
Area Percentage

Background. Strontium ranelate (StR) is an antiosteoporotic agent previously utilized for the enhancement of fracture union. We investigated the effects of StR on fracture healing using a rabbit model. Methods. Forty adult female rabbits were included in the study and were divided in 2 equal groups, according to StR treatment or untreated controls. All animals were subjected to osteotomy of the ulna, while the contralateral ulna remained intact and served as a control for the biomechanical assessment of fracture healing. Animals in the study group received 600 mg/kg/day of StR orally. All animals received ordinary food. At 2 and 4 weeks, all animals were euthanatized and the osteotomy sites were evaluated for healing through radiological, biomechanical, and histopathological studies. Results. The treatment group presented statistically significant higher callus diameter, total callus area, percentage of fibrous tissue ( p < 0.001 ), vessels/mm2, number of total vessels, and lower osteoclast number/mm2 ( p < 0.05 ) than the control group at 2 weeks. Additionally, the treatment group presented significantly higher percentages of new trabecular bone, vessels/mm2, osteoclast number/mm2, and lower values for callus diameter, as well as total callus area ( p < 0.05 ), than the control group at 4 weeks. At 4 weeks, in the treatment group, force applied ( p = 0.003 ), energy at failure ( p = 0.004 ), and load at failure ( p = 0.003 ) were all significantly higher in the forearm specimens with the osteotomized ulnae compared to those without. Radiological bone union was demonstrated for animals receiving StR at 4 weeks compared with controls ( p = 0.045 ). Conclusion. StR appears to enhance fracture healing but further studies are warranted in order to better elucidate the mechanisms and benefits of StR treatment.

scholarly journals Endogenous Production of n-3 Polyunsaturated Fatty Acids Promotes Fracture Healing in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Yuhui Chen ◽  
He Cao ◽  
Dawei Sun ◽  
Changxin Lin ◽  
Liang Wang ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Fracture Healing ◽  
Histological Analysis ◽  
Callus Formation ◽  
Healing Process ◽  
Proximal Femoral Fracture ◽  
Fracture Repair ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
X Ray

Bone fracture is a global healthcare issue for high rates of delayed healing and nonunions. Although n-3 polyunsaturated fatty acid (PUFA) is considered as a beneficial factor for bone metabolism, only few studies till date focused on the effects of n-3 PUFAs on fracture healing. In this study, we investigated the effect of endogenous n-3 PUFAs on fracture healing by measuring femur fracture repair in bothfat-1transgenic mice and WT mice. Proximal femoral fracture model was established infat-1transgenic mice and WT mice, respectively, and then the fracture was analyzed by using X-ray, micro-computed tomography (micro-CT), and histological assessment at 7, 14, 21, 28, and 35 days after fixation. The results showed that compared with WT mice,fat-1mice exhibited acceleration in fracture healing through radiographic and histological analysis (18–21 days versus 21–28 days postfracture). Meanwhile, X-ray and micro-CT analysis that showed better remodeling callus formation were in thefat-1group compared to WT group. Furthermore, histological analysis revealed that endogenous n-3 PUFAs promoted local endochondral ossification and accelerated the remodeling of calcified calluses after fracture. In conclusion, the present study indicated that endogenously produced n-3 PUFAs promote fracture healing process and accelerate bone remodeling in mice, and supplementation of n-3 PUFAs was positively associated with fracture healing.

PREDICTION OF THE TREND OF BONE FRACTURE HEALING BASED ON THE RESULTS OF THE EARLY STAGES SIMULATIONS: A FINITE ELEMENT STUDY

2019 ◽  
Vol 19 (05) ◽  
pp. 1950021
Author(s):  
JALIL NOURISA ◽  
GHOLAMREZA ROUHI
Keyword(s):  
Finite Element ◽  
Fracture Healing ◽  
Bone Fracture ◽  
Mechanical Stability ◽  
Early Stage ◽  
Healing Process ◽  
Fibrous Tissue ◽  
Fe Model ◽  
Bone Fracture Healing ◽  
Early Stages

To date, several studies have implied the importance of early stage mechanical stability in the bone fracture healing process. This study aimed at finding a correlation between the predicted different tissue phenotypes in the early stages of healing and the ultimate healing outcome. For this purpose, the process of fracture healing was numerically simulated employing an axisymmetric bi-phasic finite element (FE) model for three initial gap sizes of 1, 3 and 6[Formula: see text]mm and four initial interfragmentary strains (IFS) of 7%, 11%, 15% and 19%. The model was validated with experimental and other numerical studies from the literature. Results of this study showed that the amount of cartilage and fibrous tissue observed in the early stage after fracture can be used to qualitatively assess the outcome of complete bone healing process. Greater amount of cartilage in early stage of healing process yielded faster callus maturation, and delayed maturation of callus was predicted in the case of high fibrous tissue production. Results of this study can be used to provide an estimation of the performance of different fixation systems by considering the amounts of cartilage and fibrous tissues observed in the early stage of healing.

THE EFFECTS OF NICOTINE ON FRACTURE HEALING: AN EXPERIMENTAL STUDY

2002 ◽  
Vol 06 (02) ◽  
pp. 119-125
Author(s):  
Mustafa Agca ◽  
Ibrahim Tuncay ◽  
Nihat Tosun ◽  
Fuat Akpinar
Keyword(s):  
Fracture Healing ◽  
Healing Process ◽  
Final Analysis ◽  
Treated Group ◽  
Control Group ◽  
Significant Difference ◽  
Negative Effect ◽  
Treatment Research ◽  
Group B ◽  
Transverse Fractures

This study is aimed to evaluate treatment research the effects of nicotine on fracture healing. Thirty rats were used. Nicotine was injected subcutanously at a dosage of 2 mg/kg/day to group A and 4 mg/kg/day to group B. Control group (group C) received 0.5 ml/kg/day saline solution. Three weeks later, transverse fractures were performed on to the left ulnar and radial bones for each animal. Animals were designed to one of two follow-up groups which were euthanized either 2 or 3 weeks. On the 14th day of fracture, randomly chosen five rats from each group of A, B and C were sacrificed. At the 21st day of fracture, same procedure performed for all remaining rats. Both had surgical and histopathological evaluation were performed. Transverse fractures performed on the 21st day of study were confirmed by direct radiograms. One rat died spontaneously before completing the study was therefore excluded from the final analysis. The results indicate that healing process proceeds in each group. There was no significant difference in fracture healing between groups A and B two weeks after the fracture. However, fracture healing was significantly delayed in nicotine treated group comparing to control group. We continue to support the notion that nicotine has a negative effect on fracture healing.

scholarly journals Histological Observation Related to the Use of Laser and Ultrasound on Bone Fracture Healing

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Al-Habib MF ◽  
Salman MO ◽  
Faleh FW ◽  
Al-Ani IM
Keyword(s):  
Fracture Healing ◽  
Bone Fracture ◽  
Nd:Yag Laser ◽  
Healing Process ◽  
Complete Healing ◽  
Control Group ◽  
Albino Rats ◽  
Bone Fracture Healing ◽  
Partial Fracture ◽  
Haversian System

Objective: To study the effect of both laser and ultrasound radiation on bone fracture healing process. Materials and Methods: Nd:YAG laser (1064 nm wavelength, 135 mW power, 16 joules energy) and ultrasound (1 MHz frequency, 50 mW/cm2 power intensity) were used in this work. Fifteen mature, male, albino rats, were divided into three groups and subjected to a partial fracture on the lateral aspect of femur by a sharp blade. The fi rst group of these animals served as control group. The second group was illuminated by the Nd:YAG laser for two minutes; the fi rst dose was given immediately after surgical fracture induction; the other doses were given on days two, three, six and then one dose weekly for the next three weeks while the third group were treated by the addition of the CW ultrasound perpendicular to the laser treatment in the second group. Results: The present study showed that ultrasound increases the penetration of laser power through the tissue. The histological assessments at day 28 after the fracture of fi rst group showed incomplete healing of the bone with disfi guration and disarrangement of Haversian system and the periosteum was not yet well developed. Treatment with laser showed irregularity and lack of Haversian system formation in bone healing of the second group. The laser and ultrasound treated group (third group) expressed a complete healing at the site of fracture with a complete layer of periosteum and a well arranged Haversian system. Conclusion: Combination of laser and ultrasound in therapy can enhance healing process of a fractured bone more than laser therapy alone, as ultrasound increases the depth of laser penetration in tissue.

scholarly journals CLINICAL APPLICATION OF BONE MORPHOGENETIC PROTEIN ON FRACTURE HEALING PROCESS

2019 ◽  
Vol 6 (2) ◽  
pp. 48
Author(s):  
Riko Febrian Kunta Adjie
Keyword(s):  
Fracture Healing ◽  
Clinical Application ◽  
Bone Morphogenetic Proteins ◽  
Bone Healing ◽  
Healing Process ◽  
Delayed Union ◽  
Non Union ◽  
Morphogenetic Protein ◽  
Number Of Patients ◽  
And Function

Delayed union or non-union often occurs in bone healing fracture process. The clinical application of Bone Morphogenetic Proteins (BMPs) has helped an increasing number of patients achieve bone regeneration in clinical area lacking simple solutions for difficult bone healing situations. Many researches has proved that BMPs improved fracture healing regeneration. In this review article, introduction and current clinical issues are summarized on efficacy and function of BMPs. Currently, rhBMP-2 and rhBMP-7 have shown clinical significance but other subfamily members of BMPs still lacked of evidence.

scholarly journals The Relation between Malondialdehyde (MDA) and Histopatological Appearance in male Wistar Rats Model

1970 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Febrina Sylva Fridayanti ◽  
Erma Sulistyaningsih ◽  
Elly Nurus Sakinah
Keyword(s):  
Oxidative Stress ◽  
Fracture Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Control Group ◽  
Oxidation Stress ◽  
Treatment Groups ◽  
Male Wistar Rats

Fractures are a serious health problem in Indonesia due to increasing prevalence. The healing process of fracture is disturbed by the oxidative stress that caused by imbalance quantity of free radical and antioxidant. An antioxidant such as polyphenol, which can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of the ethanolic extract of cacao on fracture healing process in a rat model through MDA concentration and histopatological appearance. This study is in vivo experimental study with post-test only controlled group design. 30 male Wistar rats were randomized and divided into 5 groups. 1 group was rats without fractured. The negative control and three treatment groups were rats with fractured manually on left tibia under anesthesia and immobilized by bandage. The treatment groups treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW orally for 21 days. The result showed that there was a significant different between the treatment groups and the negative control group on MDA concentration and histopatological appearance (p>0,05). The corelation between them were strong and had negative direction (R=-0,771). The study concluded that cocoa ethanolic extract had a positive effect to supress oxidation stress and increases the number of osteoblast on fracture healing process. Key words: cocoa ethanolic extract, polyphenol, fracture healing process, oxidative stress

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