scholarly journals Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Steve Endeguele Ekom ◽  
Jean-De-Dieu Tamokou ◽  
Victor Kuete
Keyword(s):  
Antibacterial Activity ◽  
Capsicum Annuum ◽  
Rat Model ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Antibacterial Action ◽  
Total Phenolic ◽  
Infected Wound ◽  
Fruit Extract ◽  
Broth Microdilution Method

The wound healing process is essential to reform the damaged tissue and prevent its invasion by pathogens. The present study aims at evaluating the antibacterial and therapeutic properties of the Capsicum annuum L. (Solanaceae) extract against infected wound in a rat model with its mechanisms of antibacterial action. The fruit extract was prepared by maceration in methanol. The broth microdilution method was used to investigate the antibacterial activity of the methanol extract of C. annuum fruits. The therapeutic effect of the extract gel was performed on an excision wound infected with Staphylococcus aureus using a rat model. The total phenol, flavonoid, and tannin contents as well as the antibacterial mechanisms of action of the extract were determined using spectrophotometric methods. The C. annuum fruit extract showed antibacterial properties which can be linked to its total phenolic, flavonoid, and tannin contents. The antibacterial activity is due to the inhibition of the biofilm formation, ATPases/H+ proton pump, and dehydrogenase activity as well as the alteration of the bacterial cell membrane through the leakage of nucleic acids, reducing sugars and proteins. The extract gel showed a significant ( p < 0.05 ) increase in the percentage of wound closure and eradicated S. aureus at the infection site. The extract gel was nonirritating to the skin and slightly irritating to the eyes and should be used with caution. Overall, the findings of the present study support the traditional use of the studied plant in the treatment of wounds and infectious diseases associated with the tested bacteria.

Efficacy of An Aqueous Morinda citrifolia Fruit Extract-Phytosome Gel in Treating Oral Inflammatory Ulcer in Rabbit Model

2020 ◽  
Vol 21 (15) ◽  
pp. 1699-1710
Author(s):  
Sonsawan Kongpuckdee ◽  
Suwipa Ungphaiboon ◽  
Supreedee Sungkharak ◽  
Narubodee Phadoongsombut ◽  
Sirima Mahattanadul
Keyword(s):  
Ulcer Healing ◽  
Healing Process ◽  
Rabbit Model ◽  
Oral Ulcer ◽  
Poloxamer 407 ◽  
Gelling Agent ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Topical Gel ◽  
Daily Application

Background: Oral inflammatory ulcers are one of the common complaints of patients attending out-patient clinics. Previous in vivo studies had shown that an Aqueous M. citrifolia Fruit Extract (AMFE) possessed anti-inflammatory and ulcer healing activities. Therefore, a standardized topical bioadhesive gel containing AMFE-phytosome was developed and determined for its oral ulcer healing efficacy in a rabbit model. Methods: The AMFE phytosome (AMFE-P) was prepared by a complexation method with the required amount of AMFE: Phosphatidylcholine: Tween 80 to weigh ratio of 2:1:0.2. Poloxamer 407 was used as a gelling agent. The oral ulcer was induced in male New Zealand white rabbits by topical application of acetic acid. Each test compound was applied to the ulcer for 10 days beginning on the second day after the ulcer induction. Complete ulcer healing on the specimen obtained on day 12 was observed histologically using the histological scoring protocol. Results: The optimized gel containing AMFE-P equivalent to AMFE 10%w/w (10%AMFE-P gel) showed the best bioadhesive gel quality, a smooth and homogeneous texture with an optimum viscosity and pH range used in human oral cavity, a good physical and chemical stability and the highest percentage cumulative release of total phenolic and scopoletin content. It was found that a daily application of 10% AMFE-P gel exerted a superior ulcer healing efficacy and a significantly rapid ulcer healing process than a twice daily application of topical gel containing AMFE 10%w/w or chlorhexidine 0.2%. Conclusion: These findings demonstrated that 10% AMFE-P gel has potential as a safe and effective alternative therapeutic agent for oral ulcers.

scholarly journals Eleucine indicaPossesses Antioxidant, Antibacterial and Cytotoxic Properties

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Adel S. Al-Zubairi ◽  
Ahmad Bustamam Abdul ◽  
Siddig Ibrahim Abdelwahab ◽  
Chew Yuan Peng ◽  
Syam Mohan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Human Cancer ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Significant Activity ◽  
Human Cancer Cell Lines

The use of evidence-based complementary and alternative medicine is increasing rapidly.Eleucine indica(EI) is traditionally used in ailments associated with liver and kidneys. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Therefore, the aim of this study was to screen the hexane, dicholoromethane, ethyl acetate (EA) and methanol extracts (MeTH) of EI for their antioxidant, antibacterial and anti-cancer effects using total phenolic contents (TPCs) and DPPH, disc diffusion method and MTT cytotoxicity assays, respectively. The MeTH was showed to have the highest TPC and scavenging activity (77.7%) on DPPH assay, followed by EA (64.5%), hexane (47.19%) and DCM (40.83%) extracts, whereas the MeTH showed no inhibitory effect on all tested bacteria strains. However, the EA extract exhibited a broad spectrum antibacterial activity against all tested bacteria exceptBacillus subtilis, in which this bacterium was found to be resistant to all EI extracts. Meanwhile, hexane extract was demonstrated to have a remarkable antibacterial activity against methicillin resistantStaphylococcus aureus(MRSA) andPseudomonas aeruginosa, while the dicholoromethane extract did not exhibit significant activity againstP. aeruginosa. None of the extracts showed significant cytotoxic activity towards MCF-7, HT-29 and CEM-SS human cancer cell lines after 72 h incubation time (IC50> 30 μg/ml). These results demonstrate that the extract prepared from the EI possesses antioxidant activityin vitroin addition to antibacterial properties. Further investigations are needed to verify the antioxidant effectsin vitroandin vivo.

Antibacterial Activities of Biosynthesized ZnO Nanoparticles Using Leaf and Fruit Extracts of Neolamarckia cadamba

2021 ◽  
Vol 21 (12) ◽  
pp. 6168-6182
Author(s):  
Saee Gharpure ◽  
Rachana Yadwade ◽  
Shuana Mehmood ◽  
Balaprasad Ankamwar
Keyword(s):  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Fruit Extract ◽  
E Coli ◽  
Fruit Extracts ◽  
Before And After ◽  
Micro Organisms ◽  
Antibacterial Potential

Zinc oxide nanoparticles have been biosynthesized with the help of Neolamarckia cadamba leaf and fruit extracts. ZnO nanoparticles were tested for antibacterial activity before and after calcination against Gram positive (Staphylococcus aureus, Bacillus subtilis) as well as Gram negative micro-organisms (Escherichia coli, Pseudomonas aeruginosa) within the concentration range 0.625–10 mg/mL with the help of well diffusion technique. Higher antibacterial potential has been observed in ZnO nanoparticles synthesized using leaf extract in comparison with those synthesized using fruit extract. Increased antibacterial activity was observed before calcination as compared to after calcination. ZnO synthesized using leaf extract were observed to show significant antibacterial potential against E. coli, S. aureus along with P. aeruginosa before calcination as well as against E. coli after calcination. Similarly, ZnO nanoparticles synthesized using fruit extract exhibited antibacterial activity against E. coli and P. aeruginosa before calcination and against E. coli after calcination. Both the ZnO nanoparticles before and after calcination did not show any antibacterial activity against B. subtilis. Thus, ZnO nanoparticles can serve a dual purpose by its application as an antibacterial agent against susceptible micro-organisms as well as biocompatible carrier system for drug delivery applications in case of non-antibacterial properties by virtue of its inertness as well as easy disposal.

scholarly journals New Insights for Red Propolis of Alagoas—Chemical Constituents, Topical Membrane Formulations and Their Physicochemical and Biological Properties

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5811
Author(s):  
Valdemir C. Silva ◽  
Abiane M. G. S. Silva ◽  
Jacqueline A. D. Basílio ◽  
Jadriane A. Xavier ◽  
Ticiano G. do Nascimento ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Healing Process ◽  
Biological Properties ◽  
Biologically Active ◽  
Superior Performance ◽  
Total Phenolic ◽  
Wound Healing Process ◽  
Antioxidant Power ◽  
Hydrogel Membranes

The main objectives of this study were to evaluate the chemical constitution and allergenic potential of red propolis extract (RPE). They were evaluated, using high performance liquid chromatography (HPLC) and the release of β-hexosaminidase, respectively. A plethora of biologically active polyphenols and the absence of allergic responses were evinced. RPE inhibited the release of β-hexosaminidase, suggesting that the extract does not stimulate allergic responses. Additionally, the physicochemical properties and antibacterial activity of hydrogel membranes loaded with RPE were analyzed. Bio-polymeric hydrogel membranes (M) were obtained using 5% carboxymethylcellulose (M1 and M2), 1.0% of citric acid (M3) and 10% RPE (for all). Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenolic content, phenol release test and, antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric Reducing Antioxidant Power (FRAP). The latter appointed to the similar antioxidant capacity of the M1, M2 and M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The incorporation of RPE into the matrices and the crosslinking of M3 were evinced by FTIR. There were differences in the release of phenolic compounds, with a higher release related to M1 and lower in the strongly crosslinked M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The antibacterial activity of the membranes was determined using the disc diffusion assay, in comparison with controls, obtained in the same way, without RPE. The membranes elicited antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis, with superior performance over M3. The hydrogel membranes loaded with RPE promote a physical barrier against bacterial skin infections and may be applied in the wound healing process.

scholarly journals Comparison extraction methods for evaluating antioxidant and antibacterial properties of Vernonia amygdalina leaves extract

2020 ◽  
Vol 10 (8) ◽  
pp. 775
Author(s):  
Muharni Muharni ◽  
Elfita Elfita ◽  
Heni Yohandini ◽  
Chika Valenta
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Extraction Methods ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Flavonoid Content

In this study, we were interested in comparing the influence of different extraction methods on antioxidant and antibacterial activities of <em>V.amygdalina</em> leaves extracts. The extracts were also analyzed for their total phenolic and flavonoid content.  The extraction methods used maceration, soxhlet, and fractionation, the evaluation of antioxidant activity using DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and antibacterial activity by agar diffusion method. The total phenolic and flavonoids are determined by the spectrophotometric method.  Ethanol extract by fractionating on this method showed the highest antioxidant properties compared to other extracts with IC<sub>50 </sub>170 μg/mL in category potent antioxidant with total phenolic content 28.83 ± 2.62 mg GAE/g, and the total flavonoid content 18.78 ± 0.15 mg QE/g. Evaluation of antibacterial properties the extracts of <em>V. amyigdalina</em> showed moderate antibacterial activity against all bacteria tests with inhibition zone  6.1 ± 0.1 to  9.4 ± 0.9 mm at variation concentration 62.5 – 1000 μg/mL. The fractionation method using ethanol solvent is the best for the extraction of antioxidant compounds from <em>V. amygdalina</em>.

scholarly journals In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jamila Daraghmeh ◽  
Hamada Imtara
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Total Phenolic

The present work reveals, for the first time, the antioxidant and antibacterial properties of propolis samples collected from different regions of Palestine. The content on bioactive compounds has been estimated by total phenolic and flavone and flavonol content, while their antioxidant activity has been determined by radical scavenging methods of 1,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS), and ferric reducing power assay (FRAP). The disc diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods were carried out to evaluate the antibacterial activity of Palestinian propolis against multidrug-resistant clinical isolates, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus faecalis. The results showed that the total phenolic content ranged from 9.62 mg to 124.94 mg gallic acid equivalent GAE/g of propolis, and the flavone and flavonol content ranged from 1.06 to 75.31 mg quercetin equivalent QE/g of propolis. The samples S6 from Al-Khalil presented the strongest radical scavenging activity toward DPPH, ABTS free radicals, and FRAP assay with IC50 values of 0.02, 0.03, and 0.05 mg/mL, respectively. The results of antibacterial activity indicated that the propolis samples inhibit the growth of Gram-positive strains better than Gram-negative ones. In addition, a strong correlation was observed between the pH, resin, balsam, total phenolic, flavones and flavonol, and total antioxidant capacity (TAC) from one side and the antibacterial activity of propolis samples except on Pseudomonas aeruginosa.

Antioxidant and antibacterial activities of Pterocarya stenoptera bark extract and its mechanism on Staphylococcus aureus through cell membrane damage

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3771-3782
Author(s):  
Xuanru Li ◽  
Xiaoxi Zhang ◽  
Mengyu Xing ◽  
Ruyi Chen ◽  
Xinyu Zhao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cell Membrane ◽  
Chemical Constituents ◽  
Membrane Damage ◽  
Antibacterial Properties ◽  
Bark Extract ◽  
Total Phenolic ◽  
Pterocarya Stenoptera

Pterocarya stenoptera bark extract (PSBE) was assessed for its chemical constituents, antioxidant activity, and antibacterial activity. The total phenolic content (TPC) in the PSBE was 272.92 ± 3.22 mg gallic acid equivalent (GAE)/g. Three phenolic compounds (quercetin-3-O-(2″-O-galloyl)-rhamnoside, quercetin, and juglone) were identified in the PSBE via ultraperformance liquid chromatography/mass spectroscopy (UPLC/MS) analysis. The PSBE possessed remarkable antioxidant activity in the DPPH and ABTS+ radicals scavenging system, with IC50 values of 96.25 ± 3.82 μg/mL and 158.26 ± 6.08 μg/mL, respectively. Moreover, the PSBE had high antibacterial activity, which was especially sensitive to Staphylococcus aureus. The antibacterial mechanism of the PSBE on the S. aureus was related to the deformation of the cell morphology and the destruction of the cell membrane structure, which was confirmed using a BacLight Viability Kit and scanning electron microscope (SEM) observations. These findings suggest that the strong antioxidant and antibacterial properties of the bark extract from P. stenoptera make the tree species suitable as a natural additive in the pharmaceutical, food, and cosmetic industries.

scholarly journals Chitosan-Coated Poly(lactic acid) Nanofibres Loaded with Essential Oils for Wound Healing

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2582
Author(s):  
Giulia Milanesi ◽  
Barbara Vigani ◽  
Silvia Rossi ◽  
Giuseppina Sandri ◽  
Elisa Mele
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Essential Oils ◽  
Cellular Proliferation ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Poly Lactic Acid ◽  
Mechanical Resistance

Chronic skin wounds are characterised by a non-healing process that makes necessary the application of wound dressings on the damaged area to promote and facilitate the recovery of skin’s physiological integrity. The aim of the present work is to develop a bioactive dressing that, once applied on the injured tissue, would exert antibacterial activity and promote adhesion and proliferation of fibroblasts. Nanofibres consisting of poly(lactic acid) (PLA) and essential oils (EOs) were electrospun and coated with a medium molecular weight chitosan (CS). Black pepper essential oil (BP-EO) or limonene (L), well-known for their antibacterial properties, were added to the PLA/acetone solution before electrospinning; phase separation phenomena occurred due to the poor solubility of the EOs in the PLA solution and led to fibres having surface nano-pores. The porous electrospun fibres were coated with CS to produce hydrophilic membranes that were easy to handle, biocompatible, and suited to promote cellular proliferation. The fibrous scaffolds were tested in terms of mechanical resistance, wettability, antibacterial activity, in-vitro cytotoxicity, and ability to promote fibroblasts’ adhesion and proliferation. The results obtained proved that the CS coating improved the hydrophilicity of the fibrous mats, enhanced EO’s antibacterial potential, and promoted cell adhesion and proliferation.

scholarly journals 1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4558
Author(s):  
Virpi Virjamo ◽  
Pia Fyhrquist ◽  
Akseli Koskinen ◽  
Anu Lavola ◽  
Katri Nissinen ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Picea Abies ◽  
Solid Phase ◽  
Antibacterial Properties ◽  
Model Organism ◽  
Phase Partitioning ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Streptococcus Equi ◽  
Full Picture

Knowledge about the defensive chemistry of coniferous trees has increased in recent years regarding a number of alkaloid compounds; in addition to phenolics and terpenes. Here, we show that Norway spruce (Picea abies (L.) H. Karst.), an important boreal zone tree species; accumulates 1,6-dehydropinidine (2-methyl-6-(2-propenyl)-1,6-piperideine) in its needles and bark. We reanalyzed previously published GC-MS data to obtain a full picture of 1,6-dehydropinidine in P. abies. 1,6-dehydropinidine appeared to especially accumulate in developing spring shoots. We used solid-phase partitioning to collect the alkaloid fraction of the sprouts and thin-layer chromatography to purify 1,6-dehydropinidine. The antibacterial properties of the 1,6-dehydropinidine fraction were tested using a broth microdilution method; with Streptococcus equi subsp. equi as a model organism. Based on our results 1,6-dehydropinidine is common in alkaloid extractions from P. abies (0.4 ± 0.03 mg g−1 dw in mature needles) and it is especially abundant in young spruce shoots (2.7 ± 0.5 mg g−1 dw). Moreover; 1,6-dehydropinidine extracted from P. abies sprouts showed mild antibacterial potential against Streptococcus equi subsp. equi (MIC 55 µg mL−1). The antibacterial activity of a plant compound thought of as an intermediate rather than an end-product of biosynthesis calls for more detailed studies regarding the biological function of these coniferous alkaloids

scholarly journals Bioactive properties of Persea lingue Ness (Lauraceae) fruit and leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Lillo ◽  
F. Carvajal-Caiconte ◽  
W. Vital ◽  
P. I. Silva Junior ◽  
E. J. Paredes-Gamero ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Leaf Extract ◽  
Leukemia Cell ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
South Central

Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent.

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