scholarly journals The Value of Vaginal Microbiome in Patients with Endometrial Hyperplasia

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Hu Zhang ◽  
Qiao Feng ◽  
Zhanpeng Zhu ◽  
Haiyan Dai ◽  
Hua Hu
Keyword(s):  
Endometrial Hyperplasia ◽  
Abnormal Uterine Bleeding ◽  
Rrna Gene ◽  
Vaginal Microbiome ◽  
Bacterial Populations ◽  
Α Diversity ◽  
Significant Difference ◽  
Proliferative Endometrium ◽  
Microbial Dna ◽  
Insight Into

Objective. To investigate the profiles of the vaginal microbiome in patients with endometrial hyperplasia and to explore the potential value of vaginal microbiome in the diagnosis of endometrial hyperplasia. Materials/Methods. 26 patients suffering from abnormal uterine bleeding (AUB) with thickened endometrium revealed by transvaginal ultrasonography were enrolled. Based on pathology, 12 patients with endometrial hyperplasia were classified as the Veh group and 14 patients with proliferative endometrium were classified as the Vne group. The vaginal samples were collected for the presence of microbial DNA by high-throughput next-generation sequencing of the 16S rRNA gene. The α-diversity and ß-diversity of vaginal microbiome were analyzed and compared between bacterial populations. The ROC curve was made to evaluate the feasibility of flora as a biomarker. Results. The diversity of vaginal microbiome in the Veh group was significantly lower than that in the Vne group ( P < 0.05 ). Lactobacillus was the most represented genus in the Veh group. The study’s t-test between the two groups showed that Lactobacillus has the only significant difference in the abundance of the first 15 genera ( P < 0.01 ). ROC analysis of the abundance of Lactobacillus showed that the area of AUC was 0.83, the sensitivity was 93.00%, and the specificity was 75.00%. Conclusion. The study offers insight into the nature of the vaginal microbiome and suggests that surveying the vaginal microbiota might be useful for detection of endometrial hyperplasia.

scholarly journals Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Yacine Amar ◽  
Ilias Lagkouvardos ◽  
Rafaela L. Silva ◽  
Oluwaseun Ayodeji Ishola ◽  
Bärbel U. Foesel ◽  
...  
Keyword(s):  
Defense Mechanisms ◽  
Bacterial Load ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Skin Microbiome ◽  
Mock Community ◽  
Skin Bacteria ◽  
Α Diversity ◽  
Microbial Turnover ◽  
Microbial Dna

Abstract Background The identification of microbiota based on next-generation sequencing (NGS) of extracted DNA has drastically improved our understanding of the role of microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living and dead microorganisms. In environments such as the skin, host defense mechanisms including antimicrobial peptides and low cutaneous pH result in a high microbial turnover, likely resulting in high numbers of dead cells present and releasing substantial amounts of microbial DNA. NGS analyses may thus lead to inaccurate estimations of microbiome structures and consequently functional capacities. Results We investigated in this study the feasibility of a Benzonase-based approach (BDA) to pre-digest unprotected DNA, i.e., of dead microbial cells, as a method to overcome these limitations, thus offering a more accurate assessment of the living microbiome. A skin mock community as well as skin microbiome samples were analyzed using 16S rRNA gene sequencing and metagenomics sequencing after DNA extraction with and without a Benzonase digest to assess bacterial diversity patterns. The BDA method resulted in less reads from dead bacteria both in the skin mock community and skin swabs spiked with either heat-inactivated bacteria or bacterial-free DNA. This approach also efficiently depleted host DNA reads in samples with high human-to-microbial DNA ratios, with no obvious impact on the microbiome profile. We further observed that low biomass samples generate an α-diversity bias when the bacterial load is lower than 105 CFU and that Benzonase digest is not sufficient to overcome this bias. Conclusions The BDA approach enables both a better assessment of the living microbiota and depletion of host DNA reads. Graphical abstract

scholarly journals A retrospective study of endometrial histopathology in abnormal uterine bleeding patients

2018 ◽  
Vol 7 (10) ◽  
pp. 4116
Author(s):  
Neha Varun ◽  
Nidhi Gupta ◽  
Sana Khan
Keyword(s):  
Retrospective Study ◽  
Endometrial Hyperplasia ◽  
Diagnostic Procedure ◽  
Abnormal Uterine Bleeding ◽  
Cost Effective ◽  
Uterine Bleeding ◽  
Age Group ◽  
Medical College ◽  
Different Types ◽  
Proliferative Endometrium

Background: Abnormal uterine bleeding (AUB) is one of the most commonly encountered gynecological problem and almost 33 % of women in gynecological outpatient department presented with abnormal uterine bleeding. Dilatation and curettage (D and C) have been the main diagnostic procedure in the evaluation of abnormal uterine bleeding patients for decades. The objective of the present study was to analyze the different types of endometrial histopathology of patients presented with the abnormal uterine bleeding and its correlation with the different types of abnormal uterine bleedingMethods: This is a retrospective study, conducted in a medical college in the Department of Obstetrics and Gynecology over a period of one year from June ’17 to June ’18. All cases of AUB more than 35 years of the age group who underwent D and C procedure were included in this study. Total 100 patients were analyzed.Results: Total 100 patients were analyzed. Age group ranges from 35-58 years and most common age group presenting with AUB was 35-39 years. The most common presenting complaint was menorrhagia 54% (54/100). Histopathology of endometrium showed non-organic causes in 80% (80/100) of AUB patients and the remaining 20% (20/100) had organic causes. Most common endometrial histopathology among non-organic causes was proliferative endometrium 43.75% (35/80) and the most common organic cause was endometrial polyp 40% (8/20). Endometrial hyperplasia was found in 30% (6/20) and endometrial carcinoma was found in 20% (4/20) of cases among organic causes.Conclusions: D and C is the useful and the cost-effective diagnostic procedure in the evaluation of AUB. Histopathological evaluation of endometrial samples is especially indicated in AUB patients to rule out carcinoma and preneoplastic conditions as histopathology is 100% diagnostic in cases of endometrial hyperplasia and carcinoma.

Diets supplemented with chickpea or its main oligosaccharide component raffinose modify faecal microbial composition in healthy adults

2010 ◽  
Vol 1 (2) ◽  
pp. 197-207 ◽  
Author(s):  
W. Fernando ◽  
J. Hill ◽  
G. Zello ◽  
R. Tyler ◽  
W. Dahl ◽  
...  
Keyword(s):  
16S Rrna ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Rflp Analysis ◽  
Healthy Adults ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Clone Libraries ◽  
Bacterial Populations ◽  
Significant Difference

The effects of diets supplemented with either chickpea or its main oligosaccharide raffinose on the composition of the faecal microbial community were examined in 12 healthy adults (18-65 years) in a randomised crossover intervention study. Subjects consumed their usual diet supplemented with soups and desserts that were unfortified, or fortified with either 200 g/d of canned chickpeas or 5 g/d of raffinose for 3 week periods. Changes in faecal bacterial populations of subjects were examined using 16S rRNA-based terminal restriction fragment length polymorphisms (T-RFLP) and clone libraries generated from the diet pools. Classification of the clone libraries and T-RFLP analysis revealed that Faecalibacterium prausnitzii, reported to be an efficient butyrate producer and a highly metabolically active bacterium in the human intestinal microbiota, was more abundant in the raffinose diet and the chickpea diet compared to the control diet. However, no significant difference was observed in the faecal total short chain fatty acid concentration or in the levels of the components (butyrate, acetate and propionate) with the chickpea diet or the raffinose diet compared to the control diet. Bifidobacterium species were detected by T-RFLP in all three diet groups and quantitative real-time PCR (qPCR) analysis showed a marginal increase in 16S rRNA gene copies of Bifidobacterium with the raffinose diet compared to control (P>0.05). The number of individuals showing TRFs for the Clostridium histolyticum - Clostridum lituseburense groups, which include pathogenic bacteria species and putrefactive bacteria, were lower in the chickpea diet compared to the other two treatments. Diet appeared to affect colonisation by a high ammonia-producing bacterial isolate which was detected in 83%, 92% and 42% of individuals in the control, raffinose and chickpea groups, respectively. Our results indicate that chickpea and raffinose have the potential to modulate the intestinal microbial composition to promote intestinal health in humans.

scholarly journals Correlation of body mass index and abnormal uterine bleeding in premenopausal women

2020 ◽  
Vol 9 (11) ◽  
pp. 4640
Author(s):  
Kumarasamy Akalyaa ◽  
Pattacheravanda Nanaiah Shakuntala ◽  
Ramaiah Renuka
Keyword(s):  
Body Mass Index ◽  
Endometrial Cancer ◽  
Body Mass ◽  
Endometrial Hyperplasia ◽  
Premenopausal Women ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Significant Difference ◽  
Menstrual Patterns ◽  
The Mean

Background: As the rate of obesity is increasing in women in the recent years, the incidence of endometrial cancer increases as the body mass index (BMI) increases. Despite the clear evidence linking endometrial cancer and obesity, there is limited public awareness of this relationship. This study was undertaken to evaluate the association of BMI and endometrial pathology in premenopausal women with abnormal uterine bleeding (AUB).Methods: An analytical case control study was conducted in 100 women between the age group of 40 to 55, with AUB in the Department of Obstetrics and Gynecology, ESIC-PGIMSR Bangalore between January 2018 and June 2019. The menstrual patterns and endometrial pattern by histopathology were analysed in women with BMI of 18.5 to 24.99 and ≥25.Results: The mean age of women participated in the study group of between 40- 55 years was 44.83. The mean duration of symptoms was 10.18 months in the cases group and 8.52 months in the control group. The menstrual patterns were comparable and there was no significant difference in both the groups. The mean endometrial thickness, mean BMI, hyperplasia with or without atypia were all higher in the cases group. The frequency of occurrence of atypical endometrial hyperplasia was higher in women with increasing BMI.Conclusions: We found increased BMI to be an important independent risk factor for the development of endometrial hyperplasia with atypia which is a precursor to endometrial carcinoma in premenopausal women with AUB. 

Sputum neutrophil elastase associates with microbiota and Pseudomonas aeruginosa in bronchiectasis

2020 ◽  
Vol 56 (4) ◽  
pp. 2000769 ◽  
Author(s):  
Martina Oriano ◽  
Andrea Gramegna ◽  
Leonardo Terranova ◽  
Giovanni Sotgiu ◽  
Imran Sulaiman ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Shannon Entropy ◽  
Real Time Pcr ◽  
Neutrophil Elastase ◽  
Stable State ◽  
Rrna Gene ◽  
Respiratory Pathogens ◽  
Α Diversity ◽  
Significant Difference

IntroductionNeutrophilic inflammation is a major driver of bronchiectasis pathophysiology, and neutrophil elastase activity is the most promising biomarker evaluated in sputum to date. How active neutrophil elastase correlates with the lung microbiome in bronchiectasis is still unexplored. We aimed to understand whether active neutrophil elastase is associated with low microbial diversity and distinct microbiome characteristics.MethodsAn observational, cross-sectional study was conducted at the bronchiectasis programme of the Policlinico Hospital in Milan, Italy, where adults with bronchiectasis were enrolled between March 2017 and March 2019. Active neutrophil elastase was measured on sputum collected during stable state, microbiota analysed through 16S rRNA gene sequencing, molecular assessment of respiratory pathogens carried out through real-time PCR and clinical data collected.ResultsAmong 185 patients enrolled, decreasing α-diversity, evaluated through the Shannon entropy (ρ −0.37, p<0.00001) and Pielou's evenness (ρ −0.36, p<0.00001) and richness (ρ −0.33, p<0.00001), was significantly correlated with increasing elastase. A significant difference in median levels of Shannon entropy as detected between patients with neutrophil elastase ≥20 µg·mL−1 (median 3.82, interquartile range 2.20–4.96) versus neutrophil elastase <20 µg·mL−1 (4.88, 3.68–5.80; p<0.0001). A distinct microbiome was found in these two groups, mainly characterised by enrichment with Pseudomonas in the high-elastase group and with Streptococcus in the low-elastase group. Further confirmation of the association of Pseudomonas aeruginosa with elevated active neutrophil elastase was found based on standard culture and targeted real-time PCR.ConclusionsHigh levels of active neutrophil elastase are associated to low microbiome diversity and specifically to P. aeruginosa infection.

scholarly journals Endometrial study by Ultrasonography and its correlation with Histopathology in Abnormal uterine bleeding

2018 ◽  
Vol 9 (2) ◽  
pp. 31-35
Author(s):  
Pravin Shrestha ◽  
Smita Shrestha ◽  
Vibha Mahato
Keyword(s):  
Endometrial Hyperplasia ◽  
Endometrial Thickness ◽  
Premenopausal Women ◽  
Abnormal Uterine Bleeding ◽  
Endometrial Biopsy ◽  
Uterine Bleeding ◽  
Common Finding ◽  
Proliferative Endometrium ◽  
Group 2 ◽  
Histopathological Workup

Background: Abnormal Uterine Bleeding is defined as any deviation from a normal menstrual pattern. It is one of the common presentation in extremes of ages. However endometrial hyperplasia and carcinoma are commoner in perimenopausal and postmenopausal women warranting investigations like ultrasonography and endometrial biopsy.Aims and Objective: The aim of the study was to note the endometrial thickness by transabdominal ultrasonography and observe the histopathological pattern in women presenting with abnormal Uterine Bleeding.Material and Methods: Premenopausal women more than 45 years of age and the postmenopausal patients, without any pelvic pathology were included in the study. Endometrial thickness was measured by transabdominal sonography and endometrial biopsy was done. Tissue obtained was sent for histopathological examination.Results: A total of 105 patients were studied. Majority (92%) of patients were premenopausal. Proliferative Endometrium (32%) was the most common finding in premenopausal and atrophic endometrium (37.5%) in postmenopausal group. Malignancy was higher in a postmenopausal group (12.5%) as compared to the premenopausal group (2%). Malignancy was not seen when endometrial thickness was less than 11mm in the premenopausal age group. Endometrial hyperplasia was also more common when the thickness was more than 11mm.In postmenopausal group12.5% of patients, had complex hyperplasia.25% had simple hyperplasia and malignancy was seen in 12.5% of patients. When endometrial thickness was less than 5 mm, hyperplasia and malignancy was not seen.Conclusion: Measurement of Endometrial thickness and histopathological workup in patients above 45 years presenting with abnormal uterine bleeding will be helpful in detecting endometrial hyperplasia and carcinoma.Asian Journal of Medical Sciences Vol.9(2) 2018 31-35

scholarly journals Potential Contribution of the Uterine Microbiome in Early Missed Abortion

2021 ◽  
Author(s):  
junjun shu ◽  
ShiXin Lin ◽  
Yu Wu ◽  
Dan Gong ◽  
Xia Zou ◽  
...  
Keyword(s):  
16S Rdna ◽  
Uterine Cavity ◽  
Normal Pregnancy ◽  
Female Reproductive Tract ◽  
Potential Contribution ◽  
Missed Abortion ◽  
Bacterial Populations ◽  
Unifrac Distance ◽  
Α Diversity ◽  
Significant Difference

Abstract BackgroundMissed abortion is a kind of pregnancy failure caused by various reasons. The etiology is complicated, and the incidence of miscarriage is increasing in recent years. Previous studies shown microbiota contributes to multi-systemic function, whereas the relationship between microbiota and early missed abortion remains unknown. This study aimed to explore the composition of uterine microbiota in missed abortion and the potential role.MethodsWe enrolled 19 patients diagnosed with missed abortion and 12 healthy pregnant who subsequently had 6-8th week pregnant. All samples were taken from the endometrial fluid by a special disposable endometrial sampler. After samples were collected, DNA was extracted and amplified. The high-throughput next-generation sequencing (MiSeq) of the 16S rDNA V3-V4 region was used to identify the present of microbiota. The α-diversity of microbiota data was used to reflect species richness and evenness within bacterial populations, β-diversity was used to reflect the shared diversity between bacterial populations, and Nonmetric Multidimensional Scaling based on Weighted Unifrac distance. Statistical was determined by use of multiple testing, including the generalized mixed-effects model.ResultsThe microbiome sequencing (16S rDNA V3-V4 region) revealed that low abundance microbiome was detected in uterine cavity of patients with missed abortion and normal pregnancy. The diversity of intrauterine microflora in patients with missed abortion was higher than that in patients with induced abortion in normal pregnancy. There was no significant difference in alpha diversity between the two groups, but a significant difference was observed in beta diversity. PCoA moment array analysis did not show significant differences. Proteobacteria and Firmicutes in missed abortion patients were significantly more than those in normal pregnancy group.ConclusionThere are low abundance of microflora in uterine cavity of missed abortion patients, and the diversity of microflora is higher than that of normal pregnancy patients. Also, Proteobacteria and Firmicutes may be potential biological markers in missed abortion. New observations may prompt further investigations to understand the potential mechanism of microbiology on pathologic human pregnancy in the future.SubjectsMicrobiology, Molecular Biology, Gynecology and Obstetrics, missed abortion, Female Reproductive Tract,

P–385 The relationship between systemic oestradiol and vaginal microbiota composition in miscarriage and normal pregnancy

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
H Fourie ◽  
M Al-Memar ◽  
A Smith ◽  
S Ng ◽  
Y Lee ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Species Level ◽  
Lactobacillus Species ◽  
Rrna Gene ◽  
Vaginal Microbiome ◽  
Microbial Composition ◽  
Bacterial Composition ◽  
Bacterial Dna ◽  
Significant Difference ◽  
Eventual Outcome

Abstract Study question Is there an association between serum oestradiol, vaginal microbial composition and pregnancy outcome in the early first trimester? Summary answer In women with a vaginal microbiome deplete of Lactobacillus species at the time of Pregnancy of Uncertain Viability (IPUV), higher serum oestradiol associates with livebirth. What is known already During pregnancy, oestradiol mediates vaginal mucosal properties and increases glycogen deposition in epithelial cells which is thought to support colonisation of Lactobacillus species. Low levels of Lactobacillus associates with adverse outcomes such as miscarriage and preterm birth. The direct relationship between systemic oestradiol and the vaginal microbiome has never been studied in pregnancy. However studies have shown a positive correlation between serum oestrone, vaginal glycogen and Lactobacillus abundance in menopausal women. Study design, size, duration This was a prospective cohort study where one-hundred women were recruited in early pregnancy at the time of IPUV and donated paired blood and vaginal samples. 40 women had an eventual miscarriage, 58 had a livebirth and two pregnancies were terminated. All 100 women donated one paired serum and vaginal sample at this time point, and 22 women with Lactobacillus depletion at the time of IPUV donated further longitudinal vaginal samples. Participants/materials, setting, methods Participants were recruited from an Early Pregnancy Unit and underwent transvaginal ultrasound assessment of their pregnancy. Serum samples were analysed with an immunoassay on a ROCHE COBAS E411 analyser for Oestradiol (pg/ml) and Progesterone (ng/ml). Bacterial DNA was extracted from paired vaginal swabs and sequenced using Illumina MiSeq sequencing of 16S rRNA gene amplicons. Main results and the role of chance Lactobacillus dominance of the vagina was associated with higher serum levels of E2 and progesterone compared to depletion (E2=398pg/ml vs 302pg/ml(p = 0.02), P4=23.1ng/ml vs 17ng/ml(p = 0.02)). E2 and P4 were positively correlated (r = 0.6, p &lt; 0.05). At species level, L. crispatus dominance associated with significantly higher levels of E2 compared to high-diversity communities (468pg/ml vs 302pg/ml(p = 0.03) but no such relationship was observed for P4. Both E2 and P4 levels were lower in women who eventually miscarried. However there was no significant difference in the vaginal bacterial composition at genera or species level at this early gestational age (P = 0.08) regardless of per vaginal bleeding. However in women with Lactobacillus depleted microbiota, livebirth was associated with significantly higher E2 levels compared to women suffering miscarriage (212pg/ml in miscarriage vs 395pg/ml in livebirth, p = 0.003) (OR = 22.4 P = 0.004). In 22 women who had Lactobacillus depletion at the time of IPUV (7 with an eventual outcome of miscarriage, and 15 with an eventual outcome of livebirth), longitudinal vaginal bacterial DNA sequencing was performed. In 7/15 women with livebirth, and higher E2 levels, the microbial composition changed to become more Lactobacillus dominant during pregnancy, whereas in those with miscarriage, only 1/7 changed to become Lactobacillus dominant. Limitations, reasons for caution In this study, serum oestradiol levels were compared to the local vaginal bacterial environment. The ideal would be to study local vaginal oestradiol, glycogen and the bacterial composition. Wider implications of the findings: In contrast to previous studies in menopause where low oestrogen levels associate with the vaginal microbial composition, this study uses the high oestradiol environment of early pregnancy to study the mechanistic relationship between oestradiol and vaginal Lactobacillus abundance. Trial registration number NA

scholarly journals Histopathological spectrum of endometrium in abnormal uterine bleeding

2018 ◽  
Vol 7 (9) ◽  
pp. 3633 ◽  
Author(s):  
Simridhi Bindroo ◽  
Monika Garg ◽  
Tajinder Kaur
Keyword(s):  
Endometrial Hyperplasia ◽  
Histopathological Examination ◽  
Age Groups ◽  
Healthy Woman ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Age Group ◽  
Proliferative Endometrium ◽  
Histopathological Workup ◽  
Endometrial Carcinomas

Background: Abnormal uterine bleeding (AUB) interferes with the quality of life of an otherwise healthy woman. Until the pathology underlying menorrhagia is, accurately diagnosed, proper therapy is hardly possible. The objective of the study was to analyze different histopathological patterns of endometrium in AUB and observe the incidence of various pathologies in different age groups and their relation to parity.Methods: This two-year prospective studywas done in the department of pathology in atertiary care centre, which included 250 cases of clinically diagnosed AUB patients were evaluated. Histopathological examination of endometrial biopsies and hysterectomy specimens were done, followed by clinical correlation.Results: Out of 250 cases of AUB, Premenopausal bleeding was seen in 216 cases (86.4%) and 34 cases (13.6%) had postmenopausal bleeding. The commonest finding observed in the study was proliferative phase endometrium (37.2%), followed by secretory endometrium (34%) and endometrial hyperplasia (16%). Disordered proliferative endometrium was seen in 2.4% of patients. Endometrial carcinoma was seen in 4 (1.6%) cases. Endometrial hyperplasia was seen mostly in the age group 41-50 years (27 cases). Two cases of endometrial carcinomas were presented after age 60 years.Conclusions: Our study revealed the highest incidence of AUB in the perimenopausal age group (41-50 years). Hence a thorough histopathological workup and clinical correlation are mandatory in cases of abnormal uterine bleeding.

scholarly journals The Rhinobiome of Exacerbated Wheezers and Asthmatics: Insights From a German Pediatric Exacerbation Network

2021 ◽  
Vol 2 ◽  
Author(s):  
Malik Aydin ◽  
Cornelius Weisser ◽  
Olivier Rué ◽  
Mahendra Mariadassou ◽  
Sandra Maaß ◽  
...  
Keyword(s):  
Bacterial Abundance ◽  
Amplicon Sequencing ◽  
Nasopharyngeal Swab ◽  
Rrna Gene ◽  
Cell Integrity ◽  
Α Diversity ◽  
Children With Asthma ◽  
16 S Rrna ◽  
Nasal Microbiota ◽  
Insight Into

Although the nose, as a gateway for organism–environment interactions, may have a key role in asthmatic exacerbation, the rhinobiome of exacerbated children with asthma was widely neglected to date. The aim of this study is to understand the microbiome, the microbial immunology, and the proteome of exacerbated children and adolescents with wheeze and asthma. Considering that a certain proportion of wheezers may show a progression to asthma, the comparison of both groups provides important information regarding clinical and phenotype stratification. Thus, deep nasopharyngeal swab specimens, nasal epithelial spheroid (NAEsp) cultures, and blood samples of acute exacerbated wheezers (WH), asthmatics (AB), and healthy controls (HC) were used for culture (n = 146), 16 S-rRNA gene amplicon sequencing (n = 64), and proteomic and cytokine analyses. Interestingly, Proteobacteria were over-represented in WH, whereas Firmicutes and Bacteroidetes were associated with AB. In contrast, Actinobacteria commonly colonized HCs. Moreover, Staphylococcaceae, Enterobacteriaceae, Burkholderiaceae, Xanthobacteraceae, and Sphingomonadaceae were significantly more abundant in AB compared to WH and HC. The α-diversity analyses demonstrated an increase of bacterial abundance levels in atopic AB and a decrease in WH samples. Microbiome profiles of atopic WH differed significantly from atopic AB, whereby atopic samples of WH were more homogeneous than those of non-atopic subjects. The NAEsp bacterial exposure experiments provided a disrupted epithelial cell integrity, a cytokine release, and cohort-specific proteomic differences especially for Moraxella catarrhalis cultures. This comprehensive dataset contributes to a deeper insight into the poorly understood plasticity of the nasal microbiota, and, in particular, may enforce our understanding in the pathogenesis of asthma exacerbation in childhood.

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