scholarly journals The Antimicrobial, Antioxidative, and Anti-Inflammatory Effects of Polycaprolactone/Gelatin Scaffolds Containing Chrysin for Regenerative Endodontic Purposes

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Mahdieh Alipour ◽  
Bahareh Pouya ◽  
Zahra Aghazadeh ◽  
Hossein SamadiKafil ◽  
Marjan Ghorbani ◽  
...  
Keyword(s):  
Antimicrobial Properties ◽  
Control Group ◽  
Bacterial Strains ◽  
Pulp Tissue ◽  
Dapi Staining ◽  
Alizarin Red ◽  
Antimicrobial Effects ◽  
Natural Substance ◽  
Agar Diffusion Test ◽  
Anti Inflammatory

The appropriate endodontic material should eliminate the infection and inflammation to provide a situation for regeneration and healing of pulp tissue besides biomineralization. Chrysin is one of the active ingredients of plant flavonoids, which has significant anti-inflammatory and antimicrobial properties. In the present study, this natural substance was evaluated for antioxidant, anti-inflammatory, and mineralization properties on dental pulp stem cells (DPSCs). SEM, FTIR, and TGA tests were used to determine the successful synthesize of chrysin-loaded scaffolds. The antimicrobial effects of the synthesized scaffold against Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis were assessed by the agar diffusion test and live/dead assay. The proliferation of DPSCs on these scaffolds was determined by the MTT assay, DAPI staining, and DNA extraction. Moreover, the antioxidant and anti-inflammation activity of chrysin-loaded scaffolds on inflamed DPSCs was evaluated. Alkaline phosphatase activity and Alizarin Red S Stain tests were done to evaluate the mineralization of DPSCs seeded on these scaffolds. The chrysin-loaded scaffolds reported antimicrobial effects against evaluated bacterial strains. The proliferation of DPSCs seeded on these scaffolds was increased significantly ( p < 0.05 ). The TNF α and DCF levels in inflamed DPSCs showed a significant decrease in the presence of chrysin-loaded scaffolds ( p < 0.05 ). The ALP activity and formation of mineralized nodules of DPSCs on these scaffolds were significantly increased compared with the control group ( p < 0.05 ). These results indicated that chrysin as an ancient therapeutic agent can accelerate the healing and regeneration of damaged pulp tissue, and this active ingredient can be a potential natural substance for regenerative endodontic procedures.

scholarly journals IL-1/TNF-αInflammatory and Anti-Inflammatory Synchronization Affects Gingival Stem/Progenitor Cells’ Regenerative Attributes

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Fan Zhang ◽  
Misi Si ◽  
Huiming Wang ◽  
Mohamed K. Mekhemar ◽  
Christof E. Dörfer ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Osteogenic Differentiation ◽  
Progenitor Cells ◽  
Type I Collagen ◽  
Basic Medium ◽  
Control Group ◽  
Type I ◽  
Alizarin Red ◽  
Cell Numbers ◽  
Anti Inflammatory

Cytokines play major roles in tissue destruction/repair. The present study investigates proliferative and osteogenic differentiation potentials of gingival mesenchymal stem/progenitor cells (G-MSCs), influenced by IL-1/TNF-αinflammatory/anti-inflammatory conditions. Human G-MSCs were isolated, characterized, and cultured in basic medium (control group, M1), in basic medium with IL-1β, TNF-α, and IFN-γ(inflammatory group, M2) and with IL-1ra/TNF-αi added to M2 (anti-inflammatory group, M3). MTT tests at days 1, 3, and 7 and CFU assay at day 12 were conducted. Osteogenic differentiation was analyzed by bone-specific transcription factors (RUNX2), alkaline phosphatase (ALP), type I collagen (Col-I), osteopontin (OPN), and osteonectin (ON) expression at days 1, 3, 7, and 14 and Alizarin red staining at day 14. At day 3, the control group showed the highest cell numbers. At day 7, cell numbers in inflammatory and anti-inflammatory group outnumbered the control group. At day 12, CFUs decreased in the inflammatory and anti-inflammatory groups, with altered cellular morphology. The anti-inflammatory group demonstrated elevated bone-specific transcription factors at 14 days. After 14 days of osteogenic induction, calcified nodules in the anti-inflammatory group were higher compared to control and inflammatory groups. For regeneration, initial inflammatory stimuli appear essential for G-MSCs’ proliferation. With inflammatory persistence, this positive effect perishes and is followed by a short-term stimulatory one on osteogenesis. At this stage, selective anti-inflammatory intervention could boost G-MSCs’ differentiation.

Comparative Evaluation of 0.1% Turmeric Mouthwash with 0.2% Chlorhexidine Gluconate in Prevention of Plaque and Gingivitis: A Clinical Study

2016 ◽  
Vol 8 (1) ◽  
pp. 16-20
Author(s):  
Divya Nagunuri ◽  
GA Babitha
Keyword(s):  
Clinical Study ◽  
Dental Plaque ◽  
Comparative Evaluation ◽  
Periodontal Diseases ◽  
Antimicrobial Properties ◽  
Chlorhexidine Gluconate ◽  
Control Group ◽  
Clinical Parameters ◽  
Intergroup Comparison ◽  
Anti Inflammatory

ABSTRACT Introduction Dental plaque has been proved by extensive research to be a paramount factor in initiation and progression of gingival and periodontal diseases. Among chemical means, chlorhexidine mouthwash is regarded as the gold standard in dentistry in prevention of dental plaque. Chlorhexidine mouthwash, though effective, has certain side effects, such as brown discoloration of teeth, oral mucosal erosion, etc. Hence, there is need of an alternative medicine enmeshed within precious traditional Indian herbal therapy which is efficient, safe, and economical. Turmeric possesses anti-inflammatory, antioxidant, antimicrobial properties along with hepatoprotective, immune stimulant, antiseptic, antimutagenic properties, etc. Objectives This study was conducted to explore and prove the clinical efficacy of 0.1% turmeric mouthwash in mild and moderate gingivitis patients. Materials and methods Sixty patients with moderate gingivitis were included and divided into two equal groups: Experimental (0.1% turmeric mouthwash) and control group (0.2% chlorhexidine gluconate). The clinical parameters evaluation was done on day 0 and day 21. Results On intragroup comparison, statistically significant reduction in clinical parameters (plaque index, gingival index, gingival bleeding index) was seen in both groups on day 21, but on intergroup comparison, no statistically significant reduction in clinical parameters was seen on day 21 between the groups. Conclusion Hence, it can be concluded that 0.1% turmeric mouthwash possesses antiplaque and anti-inflammatory properties which has been proven through clinical evaluation and it was almost equally effective when compared with 0.2% chlorhexidine gluconate mouthwash in moderate gingivitis patients. How to cite this article Nagunuri D, Babitha GA, Prakash S. Comparative Evaluation of 0.1% Turmeric Mouthwash with 0.2% Chlorhexidine Gluconate in Prevention of Plaque and Gingivitis: A Clinical Study. CODS J Dent 2016;8(1):16-20.

scholarly journals Environmentally benign fabrication of SnO2-CNT nanohybrids and their multifunctional efficiency as an adsorbent, catalyst and antimicrobial agent for water decontamination

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Md. Ahmaruzzaman ◽  
Dipyaman Mohanta ◽  
Abhijit Nath
Keyword(s):  
Arsenic Concentration ◽  
Antimicrobial Properties ◽  
Water Remediation ◽  
Chemical Compositions ◽  
Bacterial Strains ◽  
Alizarin Red ◽  
Water Decontamination ◽  
Treatment Processes ◽  
One Stop ◽  
Major Treatment

Abstract Herein, we described a biogenic, additive fee, eco-friendly synthesized SnO2-CNT nanohybrid as an efficient, re-collectable and reusable material for onsite water remediation. We demonstrated that the SnO2-CNTs can provide a one stop solution for water remediation as it effectively accomplished the major treatment tasks like adsorption, catalytic transformation/degradation and disinfection. The structural, morphological, surface chemical compositions of the nanocomposite and the adsorption, catalytic and antimicrobial properties were investigated using common characterization and instrumental techniques. The results revealed the brilliant efficiency of SnO2-CNT nanoadsorbent towards As (III) and a maximum Langmuir adsorption capacity of 106.95 mg/g was observed at high arsenite concentration (C0 = 1 mg/L). The nanoadsorbent was also found to be equally efficient in low arsenite concentration ranges (C0 = 100 μg/L) as it could bring down the arsenic concentration below maximum permissible limit. Moreover, using model pollutants like p-nitrophenol, Alizarin red S, Metronidazole, bacterial strains (Bacillus subtilis, Escherichia coli, Streptococcus pneumonia etc.), and fungal strains (Aspergillus niger and Candida albicans), the multifunctional capability of SnO2-CNT towards water decontamination has been established. Our results suggested the promising potential of hierarchical nano-heterojunctions for engineering efficient water treatment processes.

scholarly journals Rat dental pulp tissue reaction after capped with propolis derived nonflavonoid extract

2011 ◽  
Vol 23 (1) ◽  
Author(s):  
Ardo Sabir
Keyword(s):  
Dental Pulp ◽  
Chemical Substance ◽  
Tissue Reaction ◽  
Control Group ◽  
Pulp Tissue ◽  
Group I ◽  
Pulp Inflammation ◽  
Dental Pulp Tissue ◽  
Anti Inflammatory ◽  
Group Ii

Propolis is a resinous material collected by honey bees from various plants. Many types of research have shown that it has antibacterial and anti-inflammation activities. Flavonoid is the main chemical substance in propolis that inhibits bacterial growth and reduces the release of free radicals, suggesting that this component has anti-bacterial and anti-inflammatory properties. However, there is another chemical substance in propolis that shows antibacterial dan anti-inflammatory activities. The purpose of the present study was to assess the rat dental pulp tissue reaction after capped with propolis derived non-flavonoids extract. Non-flavonoids substances were purified from an ethanol extract of propolis obtained from South Sulawesi, Indonesia. A Class I cavity was prepared on the occlusal surface of the right maxillary first molar in Spraque-Dawley rats. The dental pulp was exposed and then capped with a zinc oxide-based filler as a control (Group I), or non-flavonoids propolis (Group II). Then, each cavity was filled with glass ionomer cement. The animals were sacrificed at week 1, 2, or 4. Biopsy samples were obtained, and these were stained and viewed by light microscopy. The histological examination was based on the presence of polymorph nuclear leukocytes and macrophages. The results showed that pulp inflammation occurred in both groups as early as week 1. However, the inflammation occurred in Group II was relatively milder compared to Group I at all time period. Therefore, present results suggest that application of non-flavonoids propolis extract on rat's dental pulp tissue might inhibit inflammatory process.

scholarly journals Phytochemistry, Toxicology and Therapeutic Value of Petasites hybridus Subsp. Ochroleucus (Common Butterbur) from the Balkans

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 700
Author(s):  
Tatjana Mihajilov-Krstev ◽  
Boris Jovanović ◽  
Bojan Zlatković ◽  
Jelena Matejić ◽  
Jelena Vitorović ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Traditional Medicine ◽  
Pyrrolizidine Alkaloids ◽  
Sesquiterpene Lactones ◽  
Skin Irritation ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
The Balkans ◽  
Anti Inflammatory

Petasites hybridus (Common butterbur) is extensively used in traditional medicine, and is currently gaining interest and popularity as a food supplement and for its medicinal properties. It contains a large number of active compounds of potential therapeutic activity, but also toxic pyrrolizidine alkaloids. Science-based information is needed to support the developing modern use of this plant, and to direct continued safe practice in traditional medicine. The present study focused on the essential oils from leaves and rhizomes of the understudied P. hybridus ssp. ochroleucus from the Balkans, and evaluated its phytochemistry and potential therapeutic activities (antimicrobial, antioxidant, anti-cholinesterase and anti-inflammatory), as well its toxicology potential (acute toxicity in insects and mice). We studied the essential oils, which are not commonly used in traditional practices, but have a potential for safe use since the toxic pyrrolizidine alkaloids, which are non-volatiles, are usually not present in the distilled essential oils. Pyrrolizidine alkaloids were indeed not detected in the essential oils; ingestion of the essential oils did not induce toxicity signs in mice, and topical application did not elicit skin irritation in humans. The essential oils had no antimicrobial properties against 20 pathogenic bacterial strains, but demonstrated good local anti-inflammatory activity in a Carrageenan-induced paw edema test. An insect toxicity test demonstrated that the leaf essential oil is an efficient insect repellent, and the demonstrated anti-cholinesterase activity suggests a potential for the treatment of neurological conditions. Isopetasin, a sesquiterpene found in plants of the genus Petasites, known to have anti-inflammatory effects, was present only in the rhizomes essential oil (3.9%), and sesquiterpene lactones concentrations were high, likely contributing to the antioxidant activity.

Pretreatment with Methylprednisolone Improves Myocardial Protection during On-Pump Coronary Artery Bypass Surgery

2015 ◽  
Vol 18 (4) ◽  
pp. 171 ◽  
Author(s):  
Tolga Demir ◽  
Mehmet Umit Ergenoglu ◽  
Hale Bolgi Demir ◽  
Nursen Tanrikulu ◽  
Mazlum Sahin ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Artery Bypass ◽  
Myocardial Protection ◽  
Troponin T ◽  
Artery Bypass ◽  
Control Group ◽  
Study Group ◽  
Cabg Surgery ◽  
Anti Inflammatory ◽  
Pump Coronary Artery Bypass

<strong>Background</strong>: This study was undertaken to determine whether methylprednisolone could improve myocardial protection by altering the cytokine profile toward an anti-inflammatory course in patients undergoing elective coronary artery bypass grafting (CABG) surgery with cardiopulmonary bypass (CPB).<br /><strong>Methods</strong>: Forty patients who were scheduled for elective CABG surgery were randomized into two groups: the study group (n = 20), who received 1 g of methylprednisolone intravenously before CPB, and the control group (n = 20), who underwent a standard CABG surgery without any additional medication. Blood samples were withdrawn prior to surgery (T1) and then 4 hours (T2), 24 hours (T3), and 36 hours (T4) after CPB. Plasma levels of interleukin (IL)-6, IL-10, creatine kinase isoenzyme MB (CK-MB), cardiac troponin-t (cTnT), and blood glucose as well as neutrophil counts were measured at each sampling time. <br /><strong>Results</strong>: A comparison of patients between both groups revealed significantly high levels of IL-6 in the control group at T2, T3, and T4 with respect to T1 (T2: P &lt; .001; T3: <br />P &lt; .001; T4: P &lt; .001). IL-10 levels were significantly higher in the study group at T2 compared with the control group <br />(P = .007). CK-MB levels were significantly lower in the study group than in the control group at T4 (P = .001). The increase of cTnT was higher in the control group at T3 and T4 compared with the study group (T3: P = .002; T4: P = .001).<br /><strong>Conclusions</strong>: This study demonstrates that methylprednisolone is effective for ensuring better myocardial protection during cardiac surgery by suppressing the inflammatory response via decreasing the levels of IL-6 and by increasing anti-inflammatory activity through IL-10.<br /><br />

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

Detection and Assessment of Interleukin 6 in Irreversible Pulp Inflamation

2017 ◽  
Vol 68 (2) ◽  
pp. 323-327
Author(s):  
Cristian Levente Giuroiu ◽  
Maria Vataman ◽  
Gabriel Melian ◽  
Dragos Bularda ◽  
Ludmila Lozneanu ◽  
...  
Keyword(s):  
Dental Caries ◽  
Interleukin 6 ◽  
Dental Pulp ◽  
The Other ◽  
Control Group ◽  
Study Group ◽  
Pulp Tissue ◽  
Immunohistochemical Technique ◽  
Systemic Distribution ◽  
Cell Zone

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.

Polyhedral Oligomeric Silsesquioxane /Platelets Rich Plasma/Gelrite-Based Hydrogel Scaffold for Bone Tissue Engineering

2020 ◽  
Vol 26 (26) ◽  
pp. 3147-3160
Author(s):  
Saeedeh Ahmadipour ◽  
Jaleh Varshosaz ◽  
Batool Hashemibeni ◽  
Leila Safaeian ◽  
Maziar Manshaei
Keyword(s):  
Bone Fractures ◽  
Compressive Strain ◽  
Gelation Time ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Local Drug Delivery ◽  
Gelling Agent ◽  
Control Group ◽  
Hydrogel Scaffold ◽  
Alizarin Red ◽  
Oligomeric Silsesquioxane

Background: Polyhedral oligomeric silsesquioxane (POSS) is a monomer with silicon structure and an internal nanometric cage. Objective: The purpose of this study was to provide an injectable hydrogel that could be easily located in open or closed bone fractures and injuries, and also to reduce the possible risks of infections caused by bone graft either as an allograft or an autograft. Methods: Various formulations of temperature sensitive hydrogels containing hydroxyapatite, Gelrite, POSS and platelets rich plasma (PRP), such as the co-gelling agent and cell growth enhancer, were prepared. The hydrogels were characterized for their injectability, gelation time, phase transition temperature and viscosity. Other physical properties of the optimized formulation including compressive stress, compressive strain and Young’s modulus as mechanical properties, as well as storage and loss modulus, swelling ratio, biodegradation behavior and cell toxicity as rheometrical parameters were studied on human osteoblast MG-63 cells. Alizarin red tests were conducted to study the qualitative and quantitative osteogenic capability of the designed scaffold, and the cell adhesion to the scaffold was visualized by scanning electron microscopy. Results: The results demonstrated that the hydrogel scaffold mechanical force and injectability were 3.34±0.44 Mpa and 12.57 N, respectively. Moreover, the scaffold showed higher calcium granules production in alizarin red staining compared to the control group. The proliferation of the cells in G4.5H1P0.03PRP10 formulation was significantly higher than in other formulations (p<0.05). Conclusion: The optimized Gelrite/Hydroxyapatite/POSS/PRP hydrogel scaffold has useful impacts on osteoblasts activity, and may be beneficial for local drug delivery in complications including a break or bone loss.

Assessment of biochemical determinants in Multiple Sclerosis patients following the oral administration of β-D-Mannuronic acid [M2000]

2020 ◽  
Vol 17 ◽  
Author(s):  
Mohamad Reza Nikouei Moghaddam ◽  
Monireh Movahedi ◽  
Maryam Bananej ◽  
Soheil Najafi ◽  
Nahid Beladi Moghadam ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Uric Acid ◽  
Vitamin D3 ◽  
Chronic Inflammatory Disease ◽  
Toxic Effects ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mannuronic Acid ◽  
Anti Inflammatory ◽  
Multiple Sclerosis Patients

Background: Multiple sclerosis is an autoimmune chronic inflammatory disease of the central nervous system that can lead to some serious disabilities. Despite using various immunomodulatory and anti-inflammatory drugs that have therapeutic effects, they cannot reduce its progression completely, and have some unwanted side effects too. The immunomodulatory and anti-inflammatory effects of the β-D-Mannuronic acid [M2000] have been proven in several surveys, and the present research was designed to determine its toxicity and therapeutic effects in MS patients. Methods: This study was performed on 15 MS patients who took 25 mg/kg/day the oral form of the β-D-Mannuronic acid for six months, and 15 healthy people as a control group. Serum levels of Urea, Creatinine, GGT, Vitamin D3, Uric acid, and Anti-Phospholipids were compared to evaluate the therapeutic and possible toxic effects of this drug after this period. Results: Non- toxic effects through the study of Urea, Creatinine, GGT, and non-significant changes in Uric acid and AntiPhospholipids levels, besides a significant rise in Vitamin, D3 levels in the M2000 treated cases were found. Conclusions: Our results suggested that β-D-Mannuronic acid is a safe drug and has no toxicity when administered orally and also has some therapeutic effects in MS patients.

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