scholarly journals Rehardening of Eroded Enamel with CPP-ACFP Paste and CO2 Laser Treatment

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Shahin Kasraei ◽  
Parmis Kasraei ◽  
Sara Valizadeh ◽  
Mohadeseh Azarsina
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
Surface Hardness ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Treatment Modalities ◽  
Control Group ◽  
Tooth Remineralization

Background. Diet and lifestyle can destroy tooth structure due to the dissolution of enamel by acidic beverages. The present study evaluated the effect of CO2 laser irradiation and CPP-ACFP (casein phosphopeptide and amorphous calcium phosphate with fluoride) paste on the remineralization of enamel eroded by carbonated soft drinks. Methods. In the present in vitro study, 46 human sound premolar teeth were sectioned mesiodistally to achieve 84 samples. Fourteen samples were assigned to the positive control group (G1), and the remaining samples were immersed in 500 mL of cola drink for 2 minutes, followed by rinsing with distilled water for 10 seconds. This procedure was carried out three times to create erosive lesions. Then, the 60 eroded samples were randomly assigned to five groups of G2 to G6 in terms of the treatment as follows: negative control (G2), CO2 laser irradiation (G3), CPP-ACFP paste (G4), CO2 laser irradiation followed by CPP-ACFP paste application (G5), and CPP-ACFP paste application followed by CO2 laser irradiation (G6). The mean surface microhardness of the enamel surface was evaluated and determined at three points for each sample. Data were analyzed with one-way ANOVA and Tukey HSD tests ( α = 0.05 ). Results. The highest and the lowest hardness values were recorded in the G1 ( 314 ± 12  kg/mm2) and G2 ( 213.7 ± 12  kg/mm2) groups, respectively. ANOVA revealed significant differences between the study groups ( P < 0.001 ). Two-by-two comparisons showed significant differences between the G2 group and the other groups, indicating the efficacy of all the treatment modalities in tooth remineralization and rehardening procedures ( P < 0.05 ). Only in group G6, the enamel microhardness was not significantly different from the G1 positive control group ( P > 0.05 ). Conclusion. Considering the parameters used in the present study, CO2 laser irradiation or CPP-ACFP paste application alone increased eroded enamel’s surface hardness; however, their sequential application was more effective in rehardening the eroded enamel’s surface to near-normal levels.

Corrosive Effect of Probiotic And Isotonic Drinks on Dental Archwires

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Fajar Kusuma Dwi Kurniawan ◽  
Dini Novita Putri ◽  
Tri Nastiti Husna ◽  
Eko Suhartono
Keyword(s):  
Lactic Acid ◽  
Treatment Group ◽  
Normal Saline ◽  
Saline Solution ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group

The aim of this in vitro study was to investigate the effect of probiotic and isotonic drinks on chromium (Cr) and nickel (Ni) release from dental archwires. The conventional stainless steel dental archwires were used in this study, with 0.8 cm diameter, and 5 cm long from each sample. Total samples are 54 pieces dental archwires. The samples then divided into two major groups: (1) the probiotic (T1); and (2) isotonic (T2) drinks. Each major group consist three subgroups, such as: for the T1: (1) T1,1: as a negative control group (samples were immersed in saline solution); (2) T1,2: as a positive control group (samples were 1% lactic acid); (3) T1,3: as a treatment group (samples were immersed in normal saline + probiotic drink); and for the T2: (1) T2,1: as a negative control group (samples were immersed in saline solution); (2) T2,2: as a positive control group (samples were immersed in 1% lactic acid); (3) T2,3: as a treatment group (samples were immersed in normal saline + isotonic drink). After the immersion testing, there was a significance difference in Cr and Ni release from dental archwires between the subgroup of treatments from both probiotic and isotonic drinks (Kruskal-Wallis test followed by Mann-Whitney test; P > 0.05). In conclusion, the present study demonstrated that both probiotic and isotonic drinks induced the releasing of Cr and Ni from dental archwires.

scholarly journals Estudio in vitro de la erosión dental asociada al chimó / In Vitro Study of Dental Erosion associated to Chimo

2017 ◽  
Vol 36 (76) ◽  
Author(s):  
Néstor Febres Giordano ◽  
Paola Gilioli Medina ◽  
Daniela Olávez Cepeda ◽  
Carlos Omaña Cepeda ◽  
Eduvigis Solórzano Navarro
Keyword(s):  
Color Change ◽  
Negative Control Group ◽  
Dental Erosion ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Experimental Group

<p><strong>ABSTRACT. </strong><strong><em>Background</em></strong>: In Venezuela, <em>chimó</em> is a blackish goo derived from tobacco leaf. Its consumption has been associated with systemic and oral diseases. <strong><em>Purpose</em>:</strong> To identify in vitro dental erosion associated to chimó exposure. <strong><em>Methods</em></strong>: The sample consisted of 30 teeth that were assigned 10 to the negative control group, another 10 to the positive control group, and 10 to the experimental group, which were exposed respectively to artificial saliva, Coca-Cola® light, and chimó for 20 weeks. Tooth surfaces were analyzed clinically and structurally, the latter through scanning electron microscope. <strong><em>Results</em></strong>: Clinical observations showed surface color change in the negative control group; color change and surface gloss loss in the positive control group; color and surface changes in the experimental group (p=0.000). Ultrastructural analysis showed no enamel surface alterations in the negative control group. Acid etching scale values were 4 for the positive control group and 3-4 for the experimental group. The latter had signs of enamel erosion. In terms of severity of damage, the experimental group was type 1, that is, enamel loss without involving dentin. <strong><em>Conclusion</em></strong>: Under these experimental conditions, chimó caused clinical and ultrastructural enamel surface loss with type 3-4 erosion patterns, and type 1 severity<strong><em>.</em></strong></p>

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

scholarly journals Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255533
Author(s):  
Victor Angelo Martins Montalli ◽  
Patrícia Rejane de Freitas ◽  
Milenna de Figueiredo Torres ◽  
Oscar de Figueiredo Torres Junior ◽  
Dienne Hellen Moutinho De Vilhena ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
High Rate ◽  
Positive Control Group ◽  
Control Group ◽  
Healthy Environment ◽  
The Mean ◽  
Petri Dishes ◽  
Uv C

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x108 CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm2 of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Effect of Black Seed (Nigella sativa) and Uziza (Piper guineense) Leaf on the Histology of the Liver of Wistar Albino Rats

2019 ◽  
pp. 1-11
Author(s):  
Okoye Ngozi Franca ◽  
Ikiriko, Favour Ibiwari
Keyword(s):  
Aqueous Extract ◽  
Nigella Sativa ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Dependent Manner ◽  
Piper Guineense ◽  
The Third ◽  
Black Seed

Aim: This study was aimed at investigating the effects of aqueous extracts of both Nigella sativa and Piper guineense on the liver enzymes; alanine amino transferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP). Also the effect of Nigella sativa and Piper guineense extracts on the histology of the liver of Wistar rat was also studied. Materials and Methods: A total of twenty five Wistar rats were used for the study. The animals were grouped into five groups, each having five animals. They were induced with sucrose and margarine to cause high sugar levels and hyperlipidemia respectively except the positive control group which was fed normal feed. The groups were: the positive control group, the negative control group which were induced without treatment, the uziza leaf group which were induced and were treated with 2 ml of aqueous extract of uziza leaf, the black seed group which were induced and were treated with 2 ml of aqueous extract of black seed, and the black seed and uziza group which were induced and were treated with 2ml of aqueous extract of black seed and 2 ml of aqueous extract of uziza leaf.  Results: The result showed that the extracts decreased the ALT and AST and ALP activities in the rats in a time dependent manner with highest decrease obtained on the third week of treatment with the extracts.  The ALT activity (U/L) on the third week of treatment showed for the, negative control (64.48 ± 0.22), uziza leaf (28.82 ± 0.12), black seed (32.65 ± 0.02), black seed and uziza leaf (16.04 ± 0.02) (p≤0.05). The decrease in activity for AST levels (U/L) on the third week of treatment, showed for the negative control (58.00 ± 0.02), uziza leaf (11.00 ± 0.01), black seed (12.00 ± 0.02), black seed and uziza leaf (8.00 ± 0.02). Conclusion: It can be concluded that both uziza leaf and black seed have hepatoprotective effect on the liver.

scholarly journals Development of Novel Fermented Almond Milk Tea and It’s Evaluation as Antidiabetic Drink

2021 ◽  
pp. 75-87
Author(s):  
Deepak Kannan ◽  
Akash Kumaran ◽  
Sanjay Venkatesan ◽  
Prabhu Sukumaran
Keyword(s):  
Diabetes Mellitus ◽  
Plasma Glucose ◽  
Sensory Analysis ◽  
Animal Studies ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Group I ◽  
Human Volunteers

Background: It is well known that almond and tea is best known to prevent Diabetes mellitus due to its abundant source of polyphenols. Also, probiotics also have been used in the treatment of Diabetes. This study is focused on the combined effect of all these three ingredients through the process of fermentation. Objective: The aim of this present study is to develop, analyse sensory parameters in human volunteers for optimisation and evaluate the antidiabetic efficiency of Fermented Almond milk tea (FAMT) both in vitro and in vivo analysis. Study Design: Development of FAMTàOptimisation of FAMT based on sensory analysis from 25 human participantsà In vitro antidiabetic analysis of FAMT extract à Animal studies. Place and Duration: The research work was conducted during November, 2019 to March, 2020 at the Department of Biotechnology, Sri Venkateswara College of Engineering, Post Bag No.1, Pennalur, Sriperumbudur Tk, Kancheepuram Dt, TN-602117, India. Materials and Methods: FAMT was prepared by optimisation of different formulation based on sensory analysis recorded from 25 healthy human volunteers. The FAMT extract was prepared and was used for the in vitro analysis and phytochemical screening. The animal study was performed with 30 Albino Wistar rats which were divided into 5 groups under preventive regimen. Group I was healthy normoglycemic control group. Group II served as positive control. Group III received metformin (350 mg/kg bw, p.o) for 28 days. Group IV received 5% Fermented almond milk for 28 days. Group V received 5% FAMT for 28thday. All groups except Group I received single dose of STZ (50 mg/kg bw, i.p) on the 29th day for the induction of Diabetes mellitus. After 7 days from induction, animals were anaesthetized and blood was drawn for the evaluation of plasma glucose and serum TG, cholesterol & insulin.   Results: It was observed that FAMT (8:2) was favoured by the participants more than other formulations. FAMT was found to contain Saponins, flavonoids and phenol. The total poly phenol of FAMT (373 ± 3.0 μg/ml) was high than Fermented almond milk (232.5 ± 2.50 μg/ml). The DPPH scavenging, α-amylase and α-glucosidase inhibiting percentage of FAMT (59 ± 4%,52 ± 3%, 50 ± 4% respectively) was high when compared to fermented almond milk (32 ± 2%,34 ± 2% and 45 ± 2% respectively). From animal studies it was significantly observed that plasma glucose (P<0.0001) was reduced, serum insulin (P<0.001) was increased, serum TG (P<0.0.001) and cholesterol (P<0.01) were reduced when compared to Positive control Group- II. Conclusion: Thus, FAMT was proved to act as a prophylactic anti-diabetic drink and was more potent than normal fermented almond milk.

scholarly journals Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: An in-vitro study

2017 ◽  
Vol 90 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Deepak Singh Kirar ◽  
Pradeep Jain ◽  
Pallav Patni
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation ◽  
Group 2 ◽  
Group 1

Background and aim: Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal walls.Methods: Fifty extracted single rooted teeth were selected for this study. After decoronation, the root canals of these teeth were prepared to the size F3 (30 no.) using rotary ProTaper file system. These samples were randomly divided into four groups. Group 1 (n=20) were filled completely with water based calcium hydroxide (CH), Group 2 (n=20) were filled with oil based CH using lentulo spiral, Group 3 (n=5) - the positive control group received the CH as intracanal medication, but no subsequent removal, Group 4 (n=5) - the negative control did not receive CH placement. Further on, Group 1 and Group 2 were divided into four sub-groups (n=5). In sub-group A we performed conventional syringe irrigation with side-vented needle sub-group B) manual dynamic agitation, sub-group C sonic agitation using endoactivator, sub-group D passive ultrasonic irrigation (PUI). Roots were split longitudinally into mesial and distal halves. Digital images of the root canal walls were acquired by a Dental Operating Microscope (DOM) and assessed by using a scoring criteria at different thirds (coronal, middle and apical) of the root canal as follows: score 1, score 2, score 3, and score 4. Data were analyzed applying one-way analysis of variance (ANOVA) and Tukey’s multiple comparison tests at a 95% confidence interval (P < 0.05).Results: Statistically significant differences were not found between the experimental groups and the negative group in any one third of the root canal (P>0.05). However, a difference did exist between the experimental groups and the positive control group (P<0.05). None of the experimental groups totally removed CH substances from root canal walls.Conclusion: Among all experimental groups, removal of CH was best achieved by sonic agitation using endoactivator followed by passive ultrasonic irrigation (PUI), manual dynamic agitation and conventional syringe irrigation with side-vented needle.

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