scholarly journals Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Pharmaceutical Product by UV-Vis Spectrophotometric Method with Kalman Filter Algorithm

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Tran Thuc Binh ◽  
Le Thi Phuong Tram ◽  
Nguyen Van Hop ◽  
Nguyen Dang Giang Chau ◽  
Nguyen Duy Luu ◽  
...  
Keyword(s):  
Kalman Filter ◽  
Low Cost ◽  
Hplc Method ◽  
Pharmaceutical Product ◽  
Losartan Potassium ◽  
Mean Values ◽  
Visual Basic For Applications ◽  
Kalman Filter Algorithm ◽  
Accuracy And Repeatability

In this paper, hydrochlorothiazide (HCT) and losartan potassium (LSP) in tablets are simultaneously determined with UV-Vis spectrophotometry and chemometrics without separation. The spectra of standard and sample solutions were recorded in the wavelength from 220 to 300 nm at 1.0 nm intervals. The concentrations of HCT and LSP in the sample solutions were computed with the Kalman filter algorithm written on the Microsoft Excel 2016 and Visual Basic for Applications (VBA) platform. The method validation was determined via the accuracy and repeatability of measurements when analyzing HCT and LSP in the Splozarsin Plus tablet and comparing the mean values of their contents in the sample with those analyzed with HPLC. The proposed method is simple with a low cost compared with the standard HPLC method.

scholarly journals NEW, GREEN AND MINIATURIZED ANALYTICAL METHOD FOR DETERMINATION OF CEFADROXIL MONOHYDRATE IN CAPSULES

2019 ◽  
Vol 3 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Bianca Marco ◽  
Ana Kogawa ◽  
Hérida Salgado
Keyword(s):  
Analytical Chemistry ◽  
Standard Sample ◽  
Low Cost ◽  
Visible Region ◽  
Hplc Method ◽  
Minimal Amount ◽  
Green Analytical Chemistry ◽  
Color Reagent ◽  
Routine Quality Control

Cefadroxil, an oral antimicrobial, presents few techniques optimized for the reduction of solvents and toxic residues and/or non-use of them. So, a quantitative, new and miniaturized method for determination of cefadroxil monohydrate in capsules has been developed and validated by spectrophotometric method in the visible region according to the international guidelines. The analyzes were performed using microplates containing 96 wells, 1 % of phenolphthalein and sodium hydroxide 0.1 M as reagent at 552 nm. The method was (i) linear in the range of 15-115 µg mL-1, (ii) selective when comparing standard, sample, adjuvants and color reagent, (iii) precise with deviations below 4 %, (iv) accurate when comparing the proposed method with the HPLC method, (v) robusts by making small and deliberate modifications to the method, (vi) besides being fast, low cost, eco-friendly and generates minimal amount of waste. The method can be applied to the routine quality control of cefadroxil monohydrate in capsules and an effective and accessible alternative that contemplates the concepts of current and sustainable green analytical chemistry.

Development and Validation of HPLC-UV Method for the Determination of a Potent Synthetic Cannabinoid THJ-2201 in Mouse Plasma and Application in a Pharmacokinetic Study

2020 ◽  
Vol 16 (4) ◽  
pp. 404-411
Author(s):  
Hassan Y. Aboul-Enein ◽  
Gamal A.E Mostafa ◽  
Haitham AlRabiah ◽  
Mohammed Al-Ramadi ◽  
Sabry M. Attia ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
High Performance ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Reversed Phase ◽  
Hplc Method ◽  
Synthetic Cannabinoid ◽  
Mean Values ◽  
Mouse Plasma

Aim: A new simple and sensitive high-Performance Liquid Chromatography (HPLC) method for the determination of a potent synthetic cannabinoid THJ-2201, has been developed and validated. Lixiviptan was used as the Internal Standard (IS). Methods: THJ-2201 and IS were extracted from mouse plasma using deproteinization procedure that uses acetonitrile followed by HPLC analysis. The separation was carried out on a reversed-phase C18 column using water and acetonitrile mixture (30:70 v/v). The flow-rate was 1.0 mL/min. Eluting of both THJ-2201 and lixivaptan was performed at 220 nm. Results: The method demonstrated linearity over a calibration range of 95 - 1500 ng/mL and the Limit of Detection (LOD) and Quantitation (LOQ) were 28 ng/mL and 91 ng/mL, respectively. The validation of the proposed method was carried out by following the US Food and Drug Administration (FDA) guidelines. Intra- and inter-day precision did not exceed 6.4%, whereas the accuracy of THJ-2201 measurements was within ±13%. Conclusion: This new method is simple and sensitive and has been applied successfully in a pharmacokinetic study of THJ-2201 in mouse plasma. The mean values of Tmax and Cmax were 0.25 h and 141.87 ± 12.11 ng/mL, respectively.

scholarly journals An Extended Kalman Filter Algorithm for Integrating GPS and Low Cost Dead Reckoning System Data for Vehicle Performance and Emissions Monitoring

2003 ◽  
Vol 56 (2) ◽  
pp. 257-275 ◽  
Author(s):  
L. Zhao ◽  
W. Y. Ochieng ◽  
M. A. Quddus ◽  
R. B. Noland
Keyword(s):  
Kalman Filter ◽  
Extended Kalman Filter ◽  
Low Cost ◽  
Dead Reckoning ◽  
Integrated System ◽  
Vehicle Performance ◽  
Performance And Emissions ◽  
System Data ◽  
Kalman Filter Algorithm ◽  
Filter Algorithms

This paper describes the features of an extended Kalman filter algorithm designed to support the navigational function of a real-time vehicle performance and emissions monitoring system currently under development. The Kalman filter is used to process global positioning system (GPS) data enhanced with dead reckoning (DR) in an integrated mode, to provide continuous positioning in built-up areas. The dynamic model and filter algorithms are discussed in detail, followed by the findings based on computer simulations and a limited field trial carried out in the Greater London area. The results demonstrate that use of the extended Kalman filter algorithm enables the integrated system employing GPS and low cost DR devices to meet the required navigation performance of the device under development.

scholarly journals Determination of four naphthalenediols in cosmetic samples by sweeping-micellar electrokinetic chromatography and a comparison with HPLC

2021 ◽  
Author(s):  
Qian Wang ◽  
Xiaobin Li ◽  
Zhihan Zheng ◽  
Huitao Liu ◽  
Yuan Gao
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Limit Of Detection ◽  
Low Cost ◽  
Hplc Method ◽  
National Standards ◽  
Sample Introduction ◽  
Electrokinetic Chromatography ◽  
Relative Standard ◽  
Limit Of Quantitation

Abstract A sweeping micellar electrokinetic chromatography (sweeping-MEKC) method was developed for the determination of 1,7-naphthalenediol, 2,3-naphthalenediol, 1,5-naphthalenediol and 2,7-naphthalenediol in cosmetics. Several parameters affecting sweeping-MEKC method were studied systematically and the separation conditions were optimized as 20 mM NaH2PO4–110 mM SDS and 40% (v/v) MeOH (pH 2.4), with −22 kV applied voltage and UV detection at 230 nm. The sample matrix is 60 mmol L−1 NaH2PO4 and sample introduction was performed at 3 psi for 6 s. Separation of the four naphthalenediols was completed in less than 17 min. Limit of detection (LOD) and limit of quantitation (LOQ) are 0.0045∼0.0094 μg mL−1 and 0.015∼0.031 μg mL−1. Linear relationship (r 2 > 0.999) is satisfactory at the range of 0.1–10 μg mL−1. The developed method has been successfully applied to the determination of the four naphthalenediols in real cosmetic samples, with recoveries in foundation, sun cream and lotion in the range of 92.3%∼106.8% and relative standard deviation (RSD) less than 4.15%. A HPLC method described in the National Standards of the People’s Republic of China was carried out for the comparison with the proposed method. The results showed that the proposed sweeping-MEKC method has the advantages of fast, low cost with comparative sensitivity.

Determination of uracil/UH2 ratio as a potential surrogate for DPD status in cancer patients presenting with severe toxicities during fluoropyrimidine treatment

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 2020-2020 ◽  
Author(s):  
C. Mercier ◽  
C. Yang ◽  
J. Ciccolini ◽  
M. Balti ◽  
A. Evrard ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Low Cost ◽  
Fatal Outcome ◽  
Hplc Method ◽  
Severe Toxicity ◽  
Ratio Measurement ◽  
Reliable Marker ◽  
Grade 3 ◽  
A Prospective Study

2020 Background: DPD deficiency is a rare pharmacogenetic syndrome leading to overexposure to fluoropyrimidine drugs, with subsequent exacerbation of toxicity and, possibly, fatal outcome in the most severe cases. Methods: We developed a simple and rapid surrogate method for evaluating the DPD status of cancer patients. This method was retrospectively validated by following 860 patients treated with fluoropyrimidines over a 3-year period at our institute. We applied the Uracil to UH2 ratio measurement as an indirect insight into DPD functionality in a subset of 120 patients (14%), who had presented with severe toxicity (grade 3–5, WHO) after fluoropyrimidine administration. We also searched for the canonical IVS14+1G>A single nucelotide permutation (SNP), which is associated with severe DPD deficiency. Results: More than 70% of the patients with severe toxicity and 80% of those with fatal outcome displayed U/UH2 ratios out of the range previously defined with a separate reference, non-toxic population thus strongly suggesting a correlation. In contrast, IVS14+1G>A SNP was not found to be a reliable marker for predicting toxicities. Conclusions: Taken together, our data indicate U/UH2 ratio appears to be frequently implicated in the occurrence of iatrogenic events with fluoropyrimidine therapy. In addition, our simple and rapid HPLC method may meet the requirements of routine screening, allowing identification of patients who might be at risk of severe toxicity during fluoropyrimidine administration, at relatively low cost. The predictive value of our test is being investigated in a prospective study with pharmacokinetic support. No significant financial relationships to disclose.

scholarly journals Analytical Eco-Scale for Assessing the Greenness of a Developed Potentiometric Method for Lomefloxacin Hydrochloride Determination in its Different Dosage Forms, Plasma, and Dissolution Medium

2019 ◽  
Vol 102 (3) ◽  
pp. 794-800 ◽  
Author(s):  
Shereen A Boltia ◽  
Aya T Soudi ◽  
Eman S Elzanfaly ◽  
Hala E Zaazaa
Keyword(s):  
Dynamic Range ◽  
Low Cost ◽  
Sample Pretreatment ◽  
Dosage Forms ◽  
Hplc Method ◽  
Potentiometric Method ◽  
Poly Vinyl Chloride ◽  
Nitrophenyl Octyl Ether ◽  
Time Analyzer

Abstract Background: Traditional methods for Lomefloxacin hydrochloride (LOM) determination involve pretreatment steps, which extend analysis time and use hazardous chemicals. Objective: The ability to provide a rapid route without sample pretreatment for quantitative determination of compounds via a low-cost instrument is a challenging task. In this work, a simple potentiometric method was developed to determine the antibacterial LOM via in-house fabricated ion selective electrodes. Methods: Different sensors were fabricated using a poly vinyl chloride-based membrane, potassium tetrakis(4-chlorophenyl) borate as a cation exchanger, and 2-Nitrophenyl octyl ether as a plasticizer (sensor 1). To increase the selectivity of sensor 1, a selective molecular recognition component 2-hydroxypropyl-β-cyclodextrin was used as ionophore (sensor 2). Results: The proposed method was validated according to International Union of Pure and Applied Chemistry recommendations, in which the proposed sensors show a linear dynamic range from 1 × 10−5 to 1 × 10−2 mol/L, with Nernstian slopes of 55.829 and 58.229 mV/decade for sensors 1 and 2, respectively. It was applied to determine LOM in bulk powder, in different dosage forms, and in plasma with no sample pretreatment. Also, the suggested method can be used as a green, in-line bench top real-time analyzer for in-process monitoring of LOM release from its tablets, under U.S. Food and Drug Administration dissolution regulations, with clear discrimination from common excipients. Results obtained by the proposed potentiometric method were compared with those obtained by a reported HPLC method. Conclusions: The proposed method is considered as a perfect alternative to traditional reported methods for LOM determination.

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