scholarly journals Simultaneous Determination of the Five Constituents in Maiwei Dihuang Pills by the HPLC-DAD Method

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Danchun Jia ◽  
Hong Cai ◽  
Yuan Ke
Keyword(s):  
Recovery Rate ◽  
Good Condition ◽  
Sample Solution ◽  
Peak Resolution ◽  
Relative Standard ◽  
Rate Test ◽  
The Stability ◽  
Linearity Test ◽  
Precision Test

Objective. The purpose of study is to establish an HPLC-DAD method for determination of the five constituents (deoxyschizandrin, γ-schizandrin, loganin, paeoniflorin, and paeonol) in Maiwei Dihuang Pills. Methods. An Agilent ZORBAX SB-C18 chromatographic column was carried out to determine the five constituents of 50% methanol extract of Maiwei Dihuang Pills. Results. It was found the chromatographic peak resolution of each component in the study sample solution was 1.5 higher than that of other peaks and no peaks appeared in the blank control solution during the same time, suggesting specificity of HPLC-DAD was well established. The linearity test indicated that deoxyschizandrin, γ-schizandrin, loganin, paeoniflorin, and paeonol were 11.6–72.3 μg/mL, 6.4–45.2 μg/mL, 35.2–237.6 μg/mL, 18.1–114.2 μg/mL, and 32.2–215.3 μg/mL, respectively, suggesting each component has a good linear relationship within its own range. Additionally, the precision of HPLC-DAD was confirmed by a precision test; the stability of the study sample solution was confirmed by a stability test; and good reproducibility of HPLC-DAD was proved by a reproducible test. The recovery rate test showed that relative standard deviation (RSD) of recovery rate in deoxyschizandrin, γ-schizandrin, loganin, paeoniflorin, and paeonol was 100.26% (1.80%), 101.39% (1.74%), 101.19% (1.76%), 102.50% (1.65%), and 102.30% (1.58%), respectively. Conclusions. HPLC-DAD used to determine the five constituents in Maiwei Dihuang Pills, and it was easier and faster to operate, showing good condition in repeatability, precision, stability, and recovery, which is a great option for quality control.

Determination of Nitrate in Water by HPLC

2013 ◽  
Vol 448-453 ◽  
pp. 406-408
Author(s):  
Jing Liu ◽  
Xiao Na Ji ◽  
Qing Kai Ren ◽  
Sheng Shu Ai ◽  
Li Jun Wan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Recovery Rate ◽  
High Performance ◽  
Separation Efficiency ◽  
Fast Analysis ◽  
Relative Standard

We established a method fordetermination of nitrate in water by High Performance Liquid Chromatography(HPLC). The sample was analysed by HPLC-ADA and was quantitated by externalstandard method after being simply processed. This methd has the advantages ofhigh separation efficiency and fast analysis. The experiment result showed thatthe linearly dependent coefficient was0.994, the recovery rate was between 98.7%～105.7%,the relative standard deviation（RSD）wasless than 2.1 %, and the lowest detectable limit is 0.01ng (S/N=1.6).

Stability-Indicating Liquid Chromatographic Procedure for Determination of Allantoin in Cosmetic Lotion

1988 ◽  
Vol 71 (2) ◽  
pp. 290-294
Author(s):  
Ramesh J Trivedi
Keyword(s):  
Degradation Products ◽  
Glyoxylic Acid ◽  
Uv Detector ◽  
Stability Indicating ◽  
Assay Procedure ◽  
Relative Standard ◽  
The Stability ◽  
Chromatographic Parameters ◽  
Liquid Chromatographic

Abstract A sensitive, specific liquid chromatographic (LC) procedure was developed for determination of allantoin [(2,5-dioxo-4--imidaazolidinyl) urea or 5-ureidohydantion] in cosmetic lotion. A reverse-phase, ionsuppression mechanism separated allantoin from interfering constituents of the sample matrix, and the compound was determined with a UV detector at 240 nm with a sensitivity limit of ((.20 mg/mL. The chromatographic parameters were optimized for retention time, efficiency, and relative response to the analyte. The assay procedure was validated with spiked laboratory-prepared samples at 100 ± 15% levels. An average recovery of 99.4% with a relative standard deviation of 1.5% (n = 7) was obtained. The stability-indicating characteristics of the method were established by recovery study (99.8%) of samples spiked with known degradation products (urea, allantoic acid, and glyoxylic acid).

Low-Temperature Electrothermal Vaporization of 8-Hydroxyquinolinate Complex for Determination of Trace Vanadium by ICP-AES

1996 ◽  
Vol 50 (6) ◽  
pp. 785-789 ◽  
Author(s):  
Shiquan Tao ◽  
Takahiro Kumamaru
Keyword(s):  
Low Temperature ◽  
Operating Conditions ◽  
Sample Introduction ◽  
Sample Solution ◽  
Heating Process ◽  
Electrothermal Vaporization ◽  
Foreign Ions ◽  
Relative Standard ◽  
Certified Values

A trace amount of vanadium was vaporized as 8-hydroxyquinolinate complex by using a low-temperature tungsten boat furnace for sample introduction in ICP-AES. Experimental results suggest that vanadium was vaporized as vanadium(III) 8-hydroxyquinolinate complex. Vanadium(V) and vanadium(IV) were reduced in the heating process before vaporization. The operating conditions were optimized, and the effects of foreign ions were investigated. The existence of tin(II) ion in sample solution was found to enhance the emission intensity of vanadium, improve the precision of the proposed method, and also suppress the interferences from other foreign ions. The detection limit in the presence of 5 μg tin(II) was determined to be 4 pg, and in the absence of tin(II), 7 pg, of vanadium. Sub-μg L−1 levels of vanadium in sample solution could be determined by the proposed method. The precisions in relative standard deviation (% RSD) for 100 pg of vanadium under the same conditions described above were 1.9% and 4.1%, respectively. The contents of vanadium in some standard steel and rock samples determined by the proposed method were in good agreement with their certified values.

scholarly journals Improved Determination of Mercury Complex with Thiomicher’s Ketone by β-Correction Spectrophotometry

2000 ◽  
Vol 83 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Hong-Wen Gao ◽  
Sheng-Yi Zhang ◽  
Su-Mei Ye
Keyword(s):  
Stability Constant ◽  
Nonionic Surfactant ◽  
Molar Absorptivity ◽  
Mercury Complex ◽  
Relative Standard ◽  
Standard Deviations ◽  
Wastewater Samples ◽  
The Stability ◽  
First Time

Abstract Determination of the mercury complex formed with Thiomicher's ketone (TMK) was improved by β-correction spectrophotometry in the presence of a nonionic surfactant at pH 5. The complex formed was Hg(TMK)2, and its true molar absorptivity is reported for the first time: εHg(TMK)2560 = 1.04 × 105 L/mol·cm. In addition, the stability constant of Hg(TMK)2 was equal to 3.64 × 1010 at an ion strength of 0.01 at 20°C. Results from analyses of wastewater samples showed that the relative standard deviations were ≤8.3%, and the recoveries of mercury ranged from 90 to 110%.

Determination of Cl- and SO42- in Boric Acid

2012 ◽  
Vol 152-154 ◽  
pp. 507-512
Author(s):  
Dian Ting Gong
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Boric Acid ◽  
Linear Relationship ◽  
Recovery Rate ◽  
Heating Time ◽  
Measuring Time ◽  
Relative Standard ◽  
Precision And Accuracy

Microcontents of Cl- and SO42- in boric acid were determined by photoelectricity turbidimetry with acid as medium. The effects of stabilizer, acidity of solution, content of precipitant, heating time and temperature as well as measuring time on the turbidity of the solution were studied. After above experiment parameters were optimized, in the measurements to determine SO42- in boric acid, the stabilizer was glycerine-ethanol (1:4), the precipitant was acidic BaCl2. The solution was shaked and heated, as a result, the adding sequence of reagents had no effects on results. The detection limit was 0.10mg/L, relative standard deviation (RSD) was less than 3%, recovery rate was 98.85%~103.00%, and measuring time last over 40min. SO42- concentration in the range of 0.33 mg/L ~0.83mg/L showed a linear relationship with turbidity. In the determination of chlorine, the stabilizer was glycerine, measuring time held in 40min, detection limit was 0.03mg/L, RSD was less than 4%, and recovery rate was 102.2%~106.5%. Cl- concentration in the range of 0.10 mg/L ~0.25mg/L showed a linear relationship with turbidity. Photoelectricity turbidimetry improve the precision and accuracy of the measurements. The method of turbidimetry to determine Cl- and SO42- in boric acid is simple, reliable, innocuous and applicable in practice.

scholarly journals Validation of Atomic Absorption Spectrometry Method for Contamination Determination of Lead (Pb) and Cadmium (Cd) in Methanol Extract and Product of Curcuma xanthorrhiza Roxb.

2018 ◽  
Vol 2 (3) ◽  
pp. 198
Author(s):  
Dedi Hanwar ◽  
Devi Eka Nitoviani ◽  
Andi Suhendi
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Methanol Extract ◽  
Absorption Spectrometry ◽  
Test Results ◽  
Spectrometry Method ◽  
Curcuma Xanthorrhiza ◽  
Linearity Test ◽  
Precision Test

<p>The validity of atomic absorption spectrometry method for lead (Pb) and cadmium (Cd) determination in methanol extract and product of Curcuma xanthorrhiza was unknown. The aim of this work is to determine the validity of atomic absorption spectrometry method for Pb and Cd determination in methanol extract and product of Curcuma xanthorrhiza. Digestion process was done by wet digestion method and its results were read by atomic absorption spectrometry at a wavelength 217.0 nm for Pb and 228.8 nm for Cd. Validation results found % recovery of Pb and Cd respectively were 87.16% ± 0.38 (RSD 0.42) and 89.10% ± 0.06 (RSD 0.07) in methanol extract of Curcuma xanthorrhiza, 92.62% ± 0.24 (RSD 0.26) and 100.16% ± 0.07 (RSD 0.07) in Curcuma xanthorrhiza product. Precision test found the RSD value for Pb in the methanol extract and product of Curcuma xanthorrhiza were 0.02 and 0.14 respectively, but the RSD value for Cd were not detected. The linearity test results of Pb and Cd standar solution were 0.9998 and 0.9979. The LOD for Pb and Cd were 0.4 ppm and 0.1 ppm, and the LOQ were 1.3 ppm and 0.5 ppm respectively. Based on the % recovery results, the method of Pb and Cd determination by atomic absorption spectrometry is valid for methanol extract and product of Curcuma xanthorrhiza. The levels of Pb in extract and product of Curcuma xanthorrhiza were 2.10 ± 0.001 mg/kg and 4.34 ± 0.001 mg/kg respectively, but the levels of Cd in both samples did not detected.</p><p> <strong><em> </em></strong></p><p> </p>

scholarly journals Colorimetric Determination of Paracetamol Using 9-Chloroacridine Reagent: Application to Pharmaceutical Formulations

2020 ◽  
Vol 63 (2) ◽  
pp. 71-78
Author(s):  
Firas Hassan Awad
Keyword(s):  
Standard Deviation ◽  
Pharmaceutical Formulations ◽  
Extraction Process ◽  
Molar Absorptivity ◽  
Colorimetric Determination ◽  
Average Recovery ◽  
Relative Standard ◽  
Recovery Percentage ◽  
The Stability

This paper aims to develop a simple, sensitive and accurate spectrophotometric method for quantitative determination of paracetamol in aqueous medium. The method is based on the reaction between the hydrolyzed paracetamol and 9-chloroacridine reagent (9-CA). The spectra of the product show maximum absorption at 436 nm. Beer's law is obeyed in the concentration range of 0.25-11 µg/mL with molar absorptivity value 5.3x103 L/mol/cm. The average recovery percentage (Rec%) is 99.27% and relative standard deviation (RSD) is :: 2.82%. In addition, the stability constant has been determined and the reaction mechanism is proposed. The method has been applied successfully for the assay of paracetamol in pharmaceutical formulations. It is found that the method does not require extraction process and it agree well with British pharmacopeia.    

scholarly journals A VALIDATED HPLC-ASSAY FOR THE DETERMINATION OF MELOXICAM IN PRESENCE OF ITS DEGRADATION PRODUCTS

2004 ◽  
Vol 72 (3) ◽  
pp. 213-220
Author(s):  
Herbert Bartsch ◽  
Angelika Eiper ◽  
Hannelore Kopelent-Frank ◽  
Jiři Procházka
Keyword(s):  
Aqueous Solutions ◽  
Degradation Products ◽  
Sample Solution ◽  
Simulated Sunlight ◽  
Hplc Assay ◽  
Stability Indicating ◽  
Photodegradation Rate ◽  
Calibration Sample ◽  
The Stability

The stability of aqueous solutions of meloxicam is studied with samples of different concentrations, and in different containers. Quantitation is carried out utilizing a validated stability indicating HPLC assay with five-point calibration. Sample solutions of meloxicam of three different concentrations (2 mg ml−1; 250 µg ml−1; 40 µg ml−1) are subjected to simulated sunlight and tested for stability. A distinct correlation of the photodegradation rate with the concentration of the sample solution was found. Furthermore, the influence of size and geometry of the containers in which the solutions were exposed to light was investigated and results compared.

New Spectrophotometric Assay for Pilocarpine

1980 ◽  
Vol 63 (4) ◽  
pp. 689-691
Author(s):  
Sawsan El-Masry ◽  
Raafat Soliman
Keyword(s):  
Standard Deviation ◽  
Nitrate Solution ◽  
Spectrophotometric Assay ◽  
Bromocresol Purple ◽  
Eye Drops ◽  
Quick Method ◽  
Decomposition Products ◽  
Relative Standard ◽  
The Stability

Abstract A quick method for the determination of pilocarpine in eye drops in the presence of decomposition products is described. The method involves complexation of the alkaloid with bromocresol purple at pH 6. After treatment with 0.1N NaOH, the liberated dye is measured at 580 nm. The method has a relative standard deviation of 1.99%, and has been successfully applied to the analysis of 2 batches of pilocarpine eye drops. The recommended method was also used to monitor the stability of a pilocarpine nitrate solution in 0.05N NaOH at 65°C. The BPC method failed to detect any significant decomposition after 2 h incubation, but the recommended method revealed 87.5% decomposition.

scholarly journals Miniaturized Fluorimetric Method for Quantification of Zinc in Dry Dog Food

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Rute C. Martins ◽  
Ana M. Pereira ◽  
Elisabete Matos ◽  
Luisa Barreiros ◽  
António J. M. Fonseca ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Low Cost ◽  
Food Samples ◽  
Dog Food ◽  
Fluorimetric Assay ◽  
Relative Standard ◽  
The Stability ◽  
Cost Determination

Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2′-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μg L−1 in sample acid digests, with a working range of 10 to 200 μg L−1, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μg L−1) and <11.7% (10 μg L−1). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.

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