scholarly journals A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Suherni Susilowati ◽  
Imam Mustofa ◽  
Wurlina Wurlina ◽  
Indah Norma Triana ◽  
Suzanita Utama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Artificial Insemination ◽  
Skim Milk ◽  
Egg Yolk ◽  
Control Group ◽  
Genetic Quality ◽  
Treatment Groups ◽  
Igf I ◽  
Complex Protein

Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

scholarly journals Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature

2017 ◽  
Vol 3 (6) ◽  
pp. 189
Author(s):  
Tri Wahyu Suprayogy ◽  
Suherni Susilowati ◽  
Tatik Hernawati
Keyword(s):  
Amino Acid ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Cold Temperature ◽  
Treatment Groups

Nowdays, the storage of buck semen in cold temperature have not satisfied yet  because in  buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of  L-Arginin amino acid in skim diluents to quality  buck’s spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck’s semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck” semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck’s semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck’s semen. Then the samples stored in cold temperature (5oC). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity  buck’s sperm. Keywords: L-Arginin, buck, cold temperature, motility, viability and membrane integrity

Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

2021 ◽  
Author(s):  
Larbi ALLAI ◽  
Xavier Druart ◽  
Pinar Terzioğlue ◽  
Noureddine Louanjli ◽  
Boubker Nasser ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Skim Milk ◽  
Butylated Hydroxytoluene ◽  
Emission Spectrometry ◽  
Control Group ◽  
Opuntia Ficus Indica ◽  
Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

scholarly journals Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

2021 ◽  
pp. 2073-2084
Author(s):  
Suherni Susilowati ◽  
Imam Mustofa ◽  
Wurlina Wurlina ◽  
Indah Norma Triana ◽  
Suzanita Utama ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Acrosome Reaction ◽  
Skim Milk ◽  
Principal Component ◽  
Egg Yolk ◽  
Insulin Like Growth Factor ◽  
Significant Difference ◽  
Complex Protein

Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen. Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters. Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables. Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

scholarly journals Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen

2020 ◽  
Vol 13 (11) ◽  
pp. 2422-2428
Author(s):  
Ani Atul Arif ◽  
Tulus Maulana ◽  
Ekayanti Mulyawati Kaiin ◽  
Bambang Purwantara ◽  
Raden Iis Arifiantini ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Skim Milk ◽  
Egg Yolk ◽  
Dna Integrity ◽  
Dilution Technique ◽  
Bull Semen ◽  
Frozen Semen ◽  
One Step

Background and Aim: Indonesia has two National Artificial Insemination centers and 17 Regional Artificial Insemination Centers. The frozen semen production techniques differed between the centers, including the type of diluent and semen dilution technique. The aim of the research was to compare the quality of frozen Limousin bull semen diluted using different techniques. Materials and Methods: Semen was collected from three sexually mature Limousin bulls using an artificial vagina. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen that had >70% motile sperm and <20% sperm abnormality was divided into three tubes and diluted with skim milk-egg yolk (SMEY) using three different dilution techniques: One-step dilution (100% SMEY with 8% glycerol) at room temperature ([RT] 20°C until 25°C) two-step dilution (50% SMEY without glycerol at RT, stored at 5°C; and 50% SMEY with 16% glycerol after 1 h stored at 5°C); and three-step dilution (50% SMEY without glycerol at RT, stored at 5°C; and 50% SMEY with 16% glycerol added twice at 1 h and 1.5 h after being stored at 5°C). The diluted semen was loaded into 0.25 mL mini straws, equilibrated, and frozen using a freezing machine. Sperm motility, viability, membranes, DNA integrity, and concentrations of malondialdehyde (MDA) and aspartate aminotransferase (AST) enzymes were evaluated after thawing. Results: The results showed that there were no significant differences in sperm motility and DNA integrity between dilutions (p>0.05). However, sperm viability and membrane intactness of one-step dilutions were higher than those of three-step dilutions. The concentrations of MDA and AST enzymes of sperm in one-step dilutions were lower than those of three-step dilutions (p<0.05). Conclusion: It was concluded that the one-step-dilution technique was better than three-step dilution for cryopreservation of Limousin bull semen.

scholarly journals Penambahan alfa-tokoferol dalam pengencer susu skim - kuning telur terhadap kualitas spermatozoa domba Sapudi yang disimpan pada suhu 5°C

2020 ◽  
Vol 9 (3) ◽  
pp. 69
Author(s):  
Satya Alysa Cahya Puspita ◽  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Abdul Samik ◽  
Indah Norma Triana ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Alpha Tocopherol ◽  
Control Treatment ◽  
Control Group ◽  
Cold Temperatures ◽  
Fresh Semen

Spermatozoa in fresh semen of Sapudi ram has a limited life span. The storage of semen in cold temperatures (5 °C) is intended to prolong the spermatozoa's life. However, storage in cold temperatures can lead to increased production of reactive oxygen species (ROS). This condition reduces the quality of spermatozoa. The purpose of this study was to determine the effect of alphatocopherol supplementation in skim milk-egg yolk extender on viability, motility, and plasma membrane integrity of Sapudi ram spermatozoa. Fresh semen derived from Sapudi ram was divided into four treatment groups. Control treatment (P0): semen was added in the extender of skim milkegg yolk without alpha-tocopherol. Three other treatments: P1, P2, and P3 semen were added in skim milk-egg yolk extender with the supplementation of 0.25, 0.5, and 1 gram alpha-tocopherol/ 100 mL extender, respectively. The results showed that the viability, motility, and integrity of the spermatozoa plasma membrane decreased gradually according to the storage length. Supplementation of skim milk-egg yolk extender with 0.5 gram of alpha-tocopherol/100 mL (P2) was able to maintain spermatozoa quality longer (p <0.05) than the control group. It can be concluded that alpha-tocopherol with a concentration of 0.5 g/100 mL of skim milk-egg yolk extender effectively maintains the quality of Sapudi ram spermatozoa in storage at 5 ° C.

scholarly journals Kualitas post-thawing semen domba Merino dalam bahan pengencer berbasis susu skim-kuning telur yang ditambah isolat crude protein Tirosine Kinase

2021 ◽  
Vol 10 (2) ◽  
pp. 39
Author(s):  
Krisna Widiantoro ◽  
Sri Pantja Madyawati ◽  
Trilas Sardjito ◽  
Tatik Hernawati ◽  
Indah Norma Triana ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Crude Protein ◽  
Protein Tyrosine Kinase ◽  
Skim Milk ◽  
Egg Yolk ◽  
Control Group ◽  
Spermatozoa Motility ◽  
Skimmed Milk ◽  
Fresh Semen

This study was conducted to determine the effect of crude protein tyrosine kinase (PTK) isolates supplementation into skim milk-egg yolk based diluent to maintain the quality of Merino ram spermatozoa.  Four ejaculates of two Merino rams were divided into two groups for the control group (P0): Merino ram semen was diluted in skimmed milk-egg yolk based diluent, and the treatment group (P1): Merino ram semen was diluted in skim milk-egg yolk based diluent contained crude PTK 1,597 mg/ml diluent. All diluted semen was equilibrated for 2 hours at 5 °C and filled into 0.25 mL French straws. The filled straws were placed on steel racks (Cooltop, Minitube) held in liquid nitrogen vapour for 10 minutes at –140 °C, immersed immediately in liquid nitrogen at –196 °C, and stored for 48 hours for  later assessment. Post-thawed semen samples were evaluated for spermatozoa motility, viability, and morphological abnormality. The results showed that the spermatozoa motility of fresh semen of Merino ram was 82.5 ± 2.89, which was qualified for freezing. The post-thawing spermatozoa motility, viability, and morphological abnormalities of Merino ram in the P1 group were 34.11 ± 3.26%, 38.00 ± 3.00%, and 12.89 ± 4.54%, respectively. It were higher (p <0.05) than the control group of 24.44 ± 2.9%, 26.67 ± 3.32%, and 21.11 ± 3.02%. It was concluded that the addition of crude PTK isolates of 1.597 mg/ml skim milk-egg yolk diluent improved the quality of post-thawed spermatozoa of Merino ram.

scholarly journals Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate

2018 ◽  
Vol 54 (2) ◽  
pp. 84 ◽  
Author(s):  
Widayati Agustina ◽  
Widjiati Widjiati ◽  
Alfiah Hayati
Keyword(s):  
Mus Musculus ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Distilled Water ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Fruit Oil

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.

The fertility of Merino ewes artificially inseminated with semen diluted in solutions based on skim milk, glucose or ribose

1972 ◽  
Vol 23 (3) ◽  
pp. 457 ◽  
Author(s):  
KR Lapwood ◽  
ICA Martin ◽  
KW Entwistle
Keyword(s):  
Glucose Solution ◽  
Skim Milk ◽  
Egg Yolk ◽  
Control Group ◽  
Sperm Density

The fertility of Merino ewes artificially inseminated with semen diluted tenfold in milk or buffered glucose solution was lower than that of a control group of ewes inseminated with the same number of spermatozoa in undiluted semen. By means of centrifugation, the concentration of spermatozoa in the insemination dose of diluted semen was raised to match that of the undiluted semen and then the effect of dilution on fertility was eliminated for the glucose-diluted semen, but not for the milk-diluted semen. Respective percentages of ewes not returning to oestrus and ewes lambing were, after insemination with undiluted semen, 60.5, 46.7; with milk-diluted semen, 55.9, 40.2 and after reconcentration 56.0, 38.0; with glucose-diluted semen, 48.2, 35.3, and after reconcentration 62.1, 46.1. In another experiment, the percentage of ewes lambing after insemination with undiluted semen, with semen diluted tenfold with glucose, and with semen diluted with a ribose mixture and chilled at 5°C for 24 hr were respectively 67.0, 58.0, and 26.1. Both diluents contained 6% (v/v) egg yolk and diluted semen samples were reconcentrated to the original sperm density of the semen immediately before insemination.

14 CONCEPTION RATE OF BUFFALO HEIFERS TREATED WITH OESTRADIOL CYPIONATE TO INDUCE OVULATION IN TIMED ARTIFICIAL INSEMINATION PROGRAMS

2014 ◽  
Vol 26 (1) ◽  
pp. 121
Author(s):  
D. G. Souza ◽  
A. Martins ◽  
E. Oba ◽  
A. S. Camargos ◽  
E. P. Moreira ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bubalus Bubalis ◽  
Control Group ◽  
San Jose ◽  
Animal Science ◽  
Treatment Groups ◽  
Chi Squared ◽  
Timed Artificial Insemination ◽  
Animal Handling ◽  
Intravaginal Device

Reducing animal handling and costs in timed artificial insemination (TAI) systems are of great interest in animal production, regardless of the species. Thus, this study was carried out to investigate the effects of administering oestradiol cypionate (EC) or hCG to induce ovulation in buffalo (Bubalus bubalis) in TAI programs. The 79 Bufalipso heifers housed at Institute of Animal Science, San José de las Lajas, Cuba, were, on average, 24 months of age with a body weight of 310 to 380 kg at the beginning of the experiment. The investigation was conducted out of the breeding season (25 June through 28 July 2012). Heifers were randomly assigned to 1 of 3 treatment groups; either hCG (control group) or EC1 and EC2, in which TAI was performed at either 48 or 56 h after the EC injection, respectively. On the first day of the treatment regime (Day 0), animals received oestradiol benzoate (2 mg i.m.; Sincrodiol®, Ourofino, Brazil), followed by the insertion of an intravaginal device containing 1 g of progesterone (Primer®, Tecnopec, São Paolo, Brazil). On Day 9, an intramuscular luteolytic dose of cloprostenol (500 μg; Ciosin®, Intervet, Brazil) and eCG (500 UI; Novormon®, Intervet) were given in all heifers along with the removal of the intravaginal device. On the same day, 0.5 mg of EC (ECP®, Pfizer, Brazil) was administered intramuscularly in the EC1and EC2 groups. Animals in the hCG group received hCG (1000 IU i.m.; Vetecor®, Hertape Calier, Spain) on Day 11 and were inseminated 14 h later. Pregnancy diagnosis was performed by ultrasound 35 days after TAI. Data were analysed by a chi-squared test, with P < 0.05 taken as significant. There were no differences among treatment groups, with values of 57.7 (15/26), 60.7 (17/28), and 64% (16/25) for the EC1, EC2, and hCG groups, respectively. In conclusion, oestradiol cypionate was effective at inducing ovulation in buffalo heifers in TAI programs comparable to the control group, as evidenced by the conception rates. In addition, animals subjected to the EC protocol pass just 3 times through the corral instead of 4 when receiving hCG, which decreases the animal handling and costs.

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