scholarly journals Low Levels of MicroRNA-10a in Cardiovascular Endothelium and Blood Serum Are Related to Human Atherosclerotic Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Jong-Tar Kuo ◽  
Hsiao-En Tsai ◽  
Ching-Ting Lin ◽  
Chih-I Lee ◽  
Pei-Ling Lee ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Healthy Subjects ◽  
Expression Patterns ◽  
Human Coronary Artery ◽  
Downstream Target ◽  
Atherosclerotic Lesions ◽  
Human Atherosclerosis ◽  
Artery Disease ◽  
Human Coronary Artery Disease

Background. MicroRNA-10a (miR-10a) inhibits transcriptional factor GATA6 to repress inflammatory GATA6/VCAM-1 signaling, which is regulated by blood flow to affect endothelial function/dysfunction. This study aimed to identify the expression patterns of miR-10a/GATA6/VCAM-1 in vivo and study their implications in the pathophysiology of human coronary artery disease (CAD), i.e., atherosclerosis. Methods. Human atherosclerotic coronary arteries and nondiseased arteries were used to detect the expressions of miR-10a/GATA6/VCAM-1 in pathogenic vs. normal conditions. In addition, sera from CAD patients and healthy subjects were collected to detect the level of circulating miR-10a. Results. The comparison of human atherosclerotic coronary arteries with nondiseased arteries demonstrated that lower levels of endothelial miR-10a are related to human atherogenesis. Moreover, GATA6/VCAM-1 (a downstream target of miR-10a) was highly expressed in the endothelium, accompanied by the reduced levels of miR-10a during the development of human atherosclerosis. In addition, CAD patients had a significantly lower concentration of miR-10a in their serum compared to healthy subjects. Conclusions. Our findings suggest that low miR-10a and high GATA6/VCAM-1 in the cardiovascular endothelium correlates to the development of human atherosclerotic lesions, suggesting that miR-10a signaling has the potential to be developed as a biomarker for human atherosclerosis.

Quantitative Analysis of Coronary Atherosclerosis by Intra Vascular Ultrasound

1997 ◽  
Vol 3 (S2) ◽  
pp. 311-312
Author(s):  
D. G. Vince ◽  
R. Shekhar ◽  
R. M. Cothren ◽  
S. E. Nissen ◽  
E. M. Tuzcu ◽  
...  
Keyword(s):  
New Technology ◽  
Three Dimensional ◽  
Plaque Morphology ◽  
Reconstruction Technique ◽  
Human Coronary Artery ◽  
Geometric Information ◽  
Clinical Syndromes ◽  
Artery Disease ◽  
Human Coronary Artery Disease

Traditional methods for studying human coronary artery disease have significant limitations. Angiography allows evaluation only of the geometry of the remaining lumen; it cannot provide information on the structural or cellular composition of the arterial wall, which is essential to understand the processes involved in the progression of atherosclerosis. Intravascular ultrasound (IVUS) imaging is a new technology that permits tomographical visualization of a cross section through the vessel wall (Fig 1 A.) The development and refinement of IVUS has provided a powerful in vivo method to assess plaque morphology. Recent clinical studies have documented its sensitivity in detecting atherosclerosis, and it is increasingly employed to assist in selecting an appropriate therapeutic intervention. Perhaps more importantly, the potential of IVUS to quantify the structure and geometry of normal and atherosclerotic coronary arteries will allow one to characterize specific lesions and to differentiate the plaques that lead to various clinical syndromes. This study presents a three-dimensional (3D) reconstruction technique suitable for clinical use that accurately preserves 3D geometric information throughout the cardiac cycle without requiring mechanical IVUS catheter withdrawal aids.

Polymorphisms in SOCS 1 and 3 and human coronary artery disease

2015 ◽  
Vol 241 (1) ◽  
pp. e94
Author(s):  
M. Schmid ◽  
J. Marti-Jaun ◽  
M. Bühler ◽  
M. Herová ◽  
M. Hersberger
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Human Coronary Artery ◽  
Artery Disease ◽  
Human Coronary Artery Disease

Impact of Mon2 monocyte-platelet aggregates on human coronary artery disease

2018 ◽  
Vol 48 (5) ◽  
pp. e12911 ◽  
Author(s):  
Richard A. Brown ◽  
Gregory Y. H. Lip ◽  
Chetan Varma ◽  
Eduard Shantsila
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Human Coronary Artery ◽  
Platelet Aggregates ◽  
Artery Disease ◽  
Human Coronary Artery Disease

Translational Physiology: Porcine models of human coronary artery disease: implications for preclinical trials of therapeutic angiogenesis

2003 ◽  
Vol 94 (5) ◽  
pp. 1689-1701 ◽  
Author(s):  
G. Chad Hughes ◽  
Mark J. Post ◽  
Michael Simons ◽  
Brian H. Annex
Keyword(s):  
Therapeutic Angiogenesis ◽  
Regional Myocardial Blood Flow ◽  
Large Animal ◽  
Human Coronary Artery ◽  
Anatomy And Physiology ◽  
Large Animal Models ◽  
Preclinical Trials ◽  
Artery Disease ◽  
And Function ◽  
Human Coronary Artery Disease

“Therapeutic angiogenesis” describes an emerging field of cardiovascular medicine whereby new blood vessels are induced to grow to supply oxygen and nutrients to ischemic cardiac or skeletal muscle. Various methods of producing therapeutic angiogenesis have been employed, including mechanical means, gene therapy, and the use of growth factors, among others. The use of appropriate large-animal models is essential if these therapies are to be critically evaluated in a preclinical setting before their use in humans, yet little has been written comparing the various available models. Over the past decade, swine have been increasingly used in studies of chronic ischemia because of their numerous similarities to humans, including minimal preexisting coronary collaterals as well as similar coronary anatomy and physiology. Consequently, this review describes the most commonly used swine models of chronic myocardial ischemia with special attention to regional myocardial blood flow and function and critically evaluates the strengths and weaknesses of each model in terms of utility for preclinical trials of angiogenic therapies.

Abstract 15108: Imaging Coronary Atherosclerosis Using [18F]FDG PET-CT: Validation Study in Pigs

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Miikka Tarkia ◽  
Antti Saraste ◽  
Christoffer Stark ◽  
Tommi Vähäsilta ◽  
Timo Savunen ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Coronary Atherosclerosis ◽  
Vessel Wall ◽  
Ex Vivo ◽  
Intimal Thickening ◽  
Fdg Uptake ◽  
Atherosclerotic Lesions ◽  
Pet Ct ◽  
18F Fdg

Introduction: Positron emission tomography (PET) with 18F-fluorodeoxyglucose ([18F]FDG) can be used to detect atherosclerotic plaque inflammation. The degree of [18F]FDG uptake in different stages of coronary atherosclerosis remains largely unknown. Thus, we studied the amount of [18F]FDG uptake and feasibility of its in vivo quantification by combination of PET and computed tomography angiography (CTA) in a pig model of atherosclerosis. Methods: In order to induce coronary atherosclerosis, diabetes was caused by streptozotocin injections in farm pigs (n=10). After 6 months on high-fat diet, pigs underwent dual gated cardiac PET and CTA to measure [18F]FDG uptake in the proximal segments of coronary arteries as maximal target to background ratio (TBR = [18F]FDG uptake normalized to blood pool). Proximal coronary segments (n=33) were harvested for ex vivo measurement of radioactivity and for histology and measurement of tracer uptake into the vessel wall by autoradiography (ARG). Results: The pigs were hyperglycemic (12.3±4.7 mmol/L) and hypercholesterolemic (12.7±5.1 mmol/L) at the end of the study. The coronary arteries showed intimal thickening (n=16 segments) and atheroma (n=10 segments). Compared with the normal vessel wall, ARG showed 1.7±0.7 times higher accumulation of [18F]FDG in intimal thickening and 4.1±2.3 in atheroma (p=0.004 and p=0.003, respectively). Ex vivo mean vessel to blood ratio of segments with atheroma was higher than non-atherosclerotic segments (2.6±1.2 vs. 1.3±0.7, p=0.04). In vivo PET imaging showed the highest TBR of 2.7. However, maximal TBR was not significantly different in segments without atherosclerosis (1.1±0.5) and either intimal thickening (1.2±0.4, p=1.0) or atheroma (1.6±0.6, p=0.4) and no correlation was seen between the segmental TBR measured by in vivo PET-CT and [18F]FDG uptake measured by either biodistribution or autoradiography. Conclusions: We found increased [18F]FDG uptake in atherosclerotic lesions. However, quantification of [18F]FDG uptake in these relatively small and early stage atherosclerotic lesions by dual gated PET and CTA was not feasible. Further studies are needed to clarify feasibility of this approach in more advanced and highly inflamed atherosclerotic coronary plaques.

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