scholarly journals Amino Acid Profiling Study of Psidium guajava L. Leaves as an Effective Treatment for Type 2 Diabetic Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Chang Xu ◽  
Xin Li ◽  
Debin Zeng ◽  
Ying Liu ◽  
Yuhang Gao ◽  
...  
Keyword(s):  
Amino Acid ◽  
Fasting Blood Glucose ◽  
Principal Component ◽  
Psidium Guajava ◽  
Diabetic Rats ◽  
New Drugs ◽  
Concentration Data ◽  
Amino Acid Profiling ◽  
Type 2 Diabetic

Type 2 diabetes mellitus (T2DM) has become a major disease threatening human health worldwide. At present, the treatment of T2DM cannot cure diabetes and is prone to many side effects. Psidium guajava L. leaves have been reported to possess hypoglycemic activity, and they have been widely used in diabetes treatment in the folk. However, the antidiabetic mechanism has not been clearly explained. Also, the change in amino acid profile can reflect a metabolic disorder and provide insights into system-wide changes in response to physiological challenges or disease processes. The study found that P. guajava L. leaves can decrease fasting blood glucose and lipid levels in type 2 diabetic rats induced by streptozotocin. Through the analysis of amino acid profiling following 20 days of gavage administration, the concentration data were modeled by principal component analysis and orthogonal partial least squares discriminant analysis to find the different metabolites and related metabolic pathways (including cysteine and methionine metabolism, valine, leucine, and isoleucine biosynthesis, phenylalanine, tyrosine, and tryptophan biosynthesis) for the explanation of the hypoglycemic mechanism of P. guajava L., which provides an experimental and theoretical basis for diabetes prediction and for the development of new drugs for the treatment of diabetes.

scholarly journals An anxiolytic drug buspirone ameliorates hyperglycemia and endothelial dysfunction in type 2 diabetic rat model

2020 ◽  
Vol 45 (4) ◽  
pp. 397-404
Author(s):  
Tugba Gurpinar Çavuşoğlu ◽  
Ertan Darıverenli ◽  
Kamil Vural ◽  
Nuran Ekerbicer ◽  
Cevval Ulman ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Endothelial Dysfunction ◽  
Vascular Function ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Insulin Levels ◽  
Type 2 Diabetic

AbstractObjectivesType 2 diabetes is a common metabolic disease and anxiety disorders are very common among diabetics. Buspirone is used in the treatment of anxiety, also having blood glucose-lowering effects. The aim of the study was to investigate the effects of buspirone on the glucose and lipid metabolism as well as vascular function in type 2 diabetic rats.MethodsA type 2-diabetic model was induced through a high-fat diet for eight weeks followed by the administration of low-dose streptozotocin (35 mg/kg, intraperitoneal) in rats. Buspirone was given at two different doses (1.5 mg/kg/d and 5 mg/kg/d) and combined with metformin (300 mg/kg/d). The fasting glucose and insulin levels, lipid profile were analyzed, and vascular response measured from the thoracic aorta was also evaluated.ResultsBoth doses of buspirone caused a significant improvement in fasting blood glucose levels. In particular, the buspirone treatment, combined with metformin, improved endothelial dysfunction and was found to be correlated with decreased nitrate/nitrite levels.ConclusionsBuspirone may be effective in the treatment of type 2 diabetes, either alone or in combination with other treatments, particularly in terms of endothelial dysfunction, inflammation and impaired blood glucose, and insulin levels.

Anti-hyperglycemic activity of Aegle marmelos (L.) corr. is partly mediated by increased insulin secretion, α-amylase inhibition, and retardation of glucose absorption

2017 ◽  
Vol 30 (1) ◽  
Author(s):  
Prawej Ansari ◽  
Nadia Afroz ◽  
Shahnaz Jalil ◽  
Sohel Bin Azad ◽  
Md. Gazi Mustakim ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Plasma Insulin Concentration ◽  
Oral Sucrose ◽  
Type 2 Diabetic

AbstractBackground:(commonly known as Bael, golden apple) was formerly described to have anti-hyperglycemic activity. The present study aimed to explore the possible effects, in depth, ofMethods:This research begins with fasting blood glucose and oral glucose tolerance test (OGTT) to evaluate the primary anti-hyperglycemic effect in chemically induced type 2 diabetic rats. Furthermore, the plasma insulin concentration and serum glucose level were studied, which include measuring the sucrose content in six different segments of the gastrointestinal (GI) tract of the rats following oral sucrose feeding. An in situ, perfused, intestinal model in rats and glucose-fiber binding assay were conducted to find the effects ofResults:Treatment of extracts suppressed blood glucose elevation after oral sucrose (2.5 g/kg) administration and significantly (p<0.05) improved oral glucose tolerance in type 2 diabetic rats.Conclusions:The findings demonstrate that anti-hyperglycemic activity of

scholarly journals Effect of Exercise Training on Gene Expression of Adiponectin and its Receptors in Testicles and Sex Hormones in Diabetic Rats

2021 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
Hadi Karami ◽  
Mohamad Parastesh ◽  
ParvinDokht Bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Male Sex ◽  
Group Resistance ◽  
Type 2 Diabetic

Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.    

scholarly journals Pyrrolidine dithiocarbamate enhances hepatic glycogen synthesis and reduces FoxO1-mediated gene transcription in type 2 diabetic rats

2012 ◽  
Vol 302 (4) ◽  
pp. E409-E416 ◽  
Author(s):  
Tienian Zhu ◽  
Ruijing Zhao ◽  
Lizhong Zhang ◽  
Michel Bernier ◽  
Jiankun Liu
Keyword(s):  
Blood Glucose ◽  
Glycogen Synthesis ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Hepatic Glycogen ◽  
Pyrrolidine Dithiocarbamate ◽  
Glycogen Deposition ◽  
Gsk 3Β ◽  
Type 2 Diabetic

The aim of the present study was to examine the effects of pyrrolidine dithiocarbamate (PDTC) on hepatic glycogen synthesis and FoxO1 transcriptional activity in type 2 diabetic rats and the mechanism underlying these effects. Fasting blood glucose and glycogen deposition, together with expressions of two key genes related to gluconeogenesis, were studied in the liver of rats fed a normal diet (NC), high-fat diet (HFD)-induced insulin-resistant rats made type 2 diabetic by a single intraperitoneal injection of streptozotocin (DM), and a DM with intervention of PDTC (DM + PDTC) for 1 wk. The phosphorylation of Akt, GSK-3β, and FoxO1 was assessed in liver extracts of fasted rats by Western blot, whereas indirect immunofluorescence staining was performed to determine the cellular distribution of FoxO1. The DM rats exhibited obvious increases in fasting blood glucose as well as decreased hepatic glycogen content compared with the NC group. Activation of the Akt/GSK-3β pathway and inactivating phosphorylation of FoxO1 were reduced greatly in DM rat livers ( P < 0.01). By contrast, PDTC treatment protected DM rats against high fasting blood glucose and hepatic glycogen deposition loss. PDTC also elicited an increase in Akt/GSK-3β signaling and subsequent inactivation and nuclear export of FoxO1 in DM rat livers, which translated into a significant reduction in the expression of two FoxO1 target genes, phospho enolpyruvate carboxykinase and glucose-6-phosphatase. This study suggests that PDTC enhances hepatic glycogen synthesis, whereas it reduces FoxO1 transcriptional activity in DM rats.

scholarly journals Protective effects of the traditional herbal formulation on oxidative stress, learning and memory in the animal model of type 2 diabetes

2020 ◽  
Vol 24 (3) ◽  
pp. 174-184
Author(s):  
Dara Dastan ◽  
◽  
Iraj Salehi ◽  
Alireza Komaki ◽  
Alireza Gharib ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Learning And Memory ◽  
Metabolic Diseases ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Herbal Formulation ◽  
Type 2 Diabetic

Introduction: Diabetes mellitus (DM) is one of the most frequent metabolic diseases that affect various body systems. Cognitive impairment caused by diabetes is gaining more acceptance and attention. In this study, we have investigated the effects of a traditionally herbal formulation (THF) on oxidative stress (OS) and cognitive deficits in type 2 diabetic rats. Methods: Thirty-six male Wistar rats were divided into six groups: control group, diabetic group, diabetic+100, 200 or 300mg/kg THF, diabetic+glibenclamide (G) 5mg/kg. Streptozotocin-nicotinamide was used to induce type-II diabetes mellitus. Spatial and passive avoidance learning and memory function were evaluated by Morris Water Maze (MWM), novel object recognition test (NORT) and open field test (OFT). The OS biomarkers were also analyzed. The THF was standardized using RP-HPLC according to phenolic and flavonoids compounds. Results: Indicated that in the diabetic treated (300mg/kg THF and G) vs. diabetic groups, body weight and insulin were significantly increased and the levels of fasting blood glucose significantly reduced. OS was improved in the treated (300mg/kg THF) groups. Furthermore, we noticed that diabetic treated groups (300mg/kg THF) vs. diabetes caused in significant decreases of the travelled distance and escape latency to find the hidden platform, also increased in the time spent and travelled distance in the target quadrant in MWM test, exploration time in NORT and total distance moved in OFT. Conclusion: These findings suggest that THF ameliorated learning and memory deficits in type 2 diabetic rats via reducing OS. THF can be used with a caution against human DM.

scholarly journals Effect of Exercise Training on Gene Expression of Adiponectin and its Receptors in Testicles and Sex Hormones in Diabetic Rats

2021 ◽  
Author(s):  
Zahra Nadi ◽  
Mohammad Bayat ◽  
Hadi Karami ◽  
Mohamad Parastesh ◽  
ParvinDokht Bayat
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Sex Hormones ◽  
Fasting Blood Glucose ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Male Sex ◽  
Group Resistance ◽  
Type 2 Diabetic

Adiponectin and its receptors are expressed in the male reproductive system, which play a role in regulating male sex hormones and fertility. Diabetes was induced by Streptozotocin-Nicotinamide (STZ-NA i.p) in rats and after performing the trainings, adiponectin gene expression and its receptors in the testis were evaluated using real time PCR, and blood serum was then used in order to assess FSH, LH and testosterone. The STZ-NA significantly increased the fasting blood glucose, gene expression of adiponectin AdipoR1 in the testicles of diabetic rats. A significant reduction in serum testosterone and LH levels were observed in the diabetic group. Resistance and endurance training decreased blood glucose, adiponectin and AdipoR1 gene, and also increased the serum testosterone and LH levels in diabetic rats. Overall, our data suggest the role played by training in improve expression of adiponectin and AdipoR1 gene by increasing the serum testosterone and LH levels in type 2 diabetic rats.    

scholarly journals Alternanthera sessilisRed Ethyl Acetate Fraction Exhibits Antidiabetic Potential on Obese Type 2 Diabetic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Kok Keong Tan ◽  
Kah Hwi Kim
Keyword(s):  
Ethyl Acetate ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Free Fatty Acid Level ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Alternanthera Sessilis ◽  
Type 2 Diabetic

The antidiabetic potential ofAlternanthera sessilisRed was investigated using the obese type 2 diabetic rats induced by high fat diet and streptozotocin. Three fractions (hexane, ethyl acetate, and water) were obtained from the crude ethanol extract ofAlternanthera sessilisRed.Alternanthera sessilisRed ethyl acetate fraction (ASEAF) was found to possess the most potent antihyperglycemic effect through oral glucose tolerance test. The ASEAF was subsequently given to the diabetic rats for two weeks. It was found that two-week administration of ASEAF reduces the fasting blood glucose level, triglyceride level, and free fatty acid level of the rats. ASEAF-treated diabetic rats showed higher pancreatic insulin content and pancreatic total superoxide dismutase activity compared to the untreated diabetic rats. Also, the insulin sensitivity indexes suggested that ASEAF ameliorates the insulin resistant state of the diabetic rats. In conclusion, ASEAF could be developed into a potential antidiabetic agent for the management of type 2 diabetes.

Effect of the Best Compatibility of Components in Corni Fructus on WT1 Expression in Glomerular Podocytes of Type 2 Diabetic Rats with Early Nephropathy

2012 ◽  
Vol 40 (03) ◽  
pp. 537-549 ◽  
Author(s):  
Hong Liu ◽  
Huiqing Xu ◽  
Cunsi Shen ◽  
Chen Wu
Keyword(s):  
Protective Effect ◽  
Wilms Tumor ◽  
Vascular Endothelial Cells ◽  
Morphological Changes ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Dose ◽  
Corni Fructus ◽  
Type 2 Diabetic

Our previous studies have shown that the best compatibility of components in Corni Fructus (PC) had a protective effect on vascular endothelial cells. In this study, the effect of PC on WT1 expression in glomerular podocytes and the mechanism of PC on early nephropathy in type 2 diabetic rats have been investigated. Type 2 diabetic rats were generated by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin (STZ), then fed aminoguanidine (100 mg/kg), glimepiride (0.4 mg/kg), low-dose (60 mg/kg) or high-dose (120 mg/kg) of PC once a day for 12 weeks. Fasting blood glucose (FBG) was examined regularly. Insulin (INS) was measured by a radioimmunoassay. Microalbuminuria (mALB) was measured by an ELISA assay. Urinary creatinine (UCr), blood urea nitrogen (BUN) and N-acetyl-β-D-glucosaminidase (NAG) were measured by colorimetric assays. Renal morphological changes were observed by optical microscopy. The expression index of Wilms tumor 1 (WT1EI) in glomeruli was examined by immunohistochemistry. The results showed that PC led to a decrease in FBG (p < 0.01), NAG (p < 0.05) and mALB (p < 0.05) and an increase in INS (p < 0.01) and WT1EI (p < 0.05) compared to the diabetic group. PC could improve renal damage and greatly reduce the renal morphology score (p < 0.05). These results suggested that PC had the protective effect on early nephropathy in type 2 diabetic rats, which was closely related to the regulation of podocytes.

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