scholarly journals Supercritical Fluid Extraction of Polyphenols from Vietnamese Callisia fragrans Leaves and Antioxidant Activity of the Extract

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Le Thao My Phan ◽  
Kim Thien Phuc Nguyen ◽  
Hoai Thanh Vuong ◽  
Do Dat Tran ◽  
Tran Xuan Phuong Nguyen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Flow Rate ◽  
Ethanol Concentration ◽  
Soxhlet Extraction ◽  
Extraction Temperature ◽  
Total Polyphenol ◽  
High Antioxidant Activity ◽  
Medicinal Properties ◽  
Temperature And Pressure ◽  
Single Factor Experiment

Vietnamese Callisia fragrans (C. fragrans) has been considered as a valuable traditional plant with various medicinal properties. In this study, polyphenols were extracted from Vietnamese C. fragrans leaves by supercritical carbon dioxide (SC-CO2) extraction method using ethanol as a cosolvent. The investigation of four factors influencing the total polyphenol content (TPC) and antioxidant activity of the extracts obtained from each single-factor experiment was conducted including ethanol concentration, CO2 flow rate, extraction temperature, and pressure. Besides, the extraction efficiency of the SC-CO2 method under the best extraction conditions, namely ethanol concentration of 14%, CO2 flow rate of 20 g/min, extraction temperature of 45°C, and pressure of 200 bar was compared to that of the Soxhlet extraction (SE) method in terms of the TPC and antioxidant activity of the extracts. The results showed that using SC-CO2 method, the TPC and the half-maximal inhibitory concentration value obtained were of 87.42 ± 1.33 mg/g and 243.83 ± 5.30 μM TE/g, respectively, with much less time and solvent amount required while that obtained using SE method were of 85.34 ± 4.27 mg/g and 236.33 ± 7.66 μM TE/g, respectively. This indicated that SC-CO2 would be suitable for the industrial production of polyphenols with high antioxidant activity of the extracts obtained due to the restrictions of using the SE method and advantages of applying SC-CO2 method. Therefore, SC-CO2 method could be regarded as a potentially upcoming extraction technique which might be employed to replace the conventional SE method.

scholarly journals Extraction Process of Polyphenols from Soybean (Glycine max L.) Sprouts: Optimization and Evaluation of Antioxidant Activity

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 489 ◽  
Author(s):  
Xuan Tien Le ◽  
Vo Luu Lan Vi ◽  
Tran Quoc Toan ◽  
Long Giang Bach ◽  
Tran Thanh Truc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Dry Weight ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Polyphenol Content ◽  
Experimental Conditions ◽  
Total Polyphenol ◽  
Total Polyphenol Content

This research aimed to optimize the total polyphenol content (TPC) extracted from soybean sprout powder under different experimental parameters, including ethanol concentration (60–100% v/v), extraction temperature (40–80 °C), extraction time (15–150 min), material:solvent ratio (1:4–1:10 g/mL), the number extraction cycles (1, 2 and 3 times), the age of sprout (0–7 days), and the used part of the sprout (cotyledon, hypocotyl, or radicle). The obtained results were used in response surface methodology, in combination with a central composite design, to model the total polyphenol content (TPC) with respect to three variables, including ethanol concentration, extraction temperature, and material:solvent ratio. The experimental conditions for optimal recovery of TPC consisted of ethanol concentration of 88% (v/v), extraction temperature of 59 °C, material:solvent ratio of 1:6.5 g/mL, extraction time of 60 min, and 2 cycles of maceration. In addition, for maximal TPC, the sprout should undergo the germination of 5 days and the radicle fraction should be used. Based on the suggested optimum conditions, the obtained and verified TPC was 19.801 mg genistein (GE)/g dry weight (d.w.). The obtained dried extract also exhibited low antioxidant activity.

scholarly journals Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2494
Author(s):  
Wei Wang ◽  
Yu-Ting Gao ◽  
Ji-Wen Wei ◽  
Yin-Feng Chen ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Ph Value ◽  
Soxhlet Extraction ◽  
Passion Fruit ◽  
Total Phenol Content ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Dpph Scavenging

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

2019 ◽  
Vol 814 ◽  
pp. 527-531
Author(s):  
Trieu Tuan Anh ◽  
Luu Tu Hao ◽  
Long Giang Bach ◽  
Duy Chinh Nguyen ◽  
Tri Duc Lam
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Herbal Plants ◽  
Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

scholarly journals Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from Akebia trifoliata Peels

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1432
Author(s):  
Min Luo ◽  
Dan-Dan Zhou ◽  
Ao Shang ◽  
Ren-You Gan ◽  
Hua-Bin Li
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Extraction Parameters

Akebia trifoliata is a fruit with rich nutritional properties, and its peel is produced as a by-product. In this research, we investigated the influences of microwave-assisted extraction parameters on antioxidant activity of the extract from Akebia trifoliata peels, and the ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) as well as total phenolic contents (TPC) were used to optimize extraction parameters. The influences of ethanol concentration, microwave power and solvent-to-material ratio, as well as extraction temperature and time on TPC, FRAP and TEAC values, were assessed using single-factor tests. Three parameters with obvious effects on antioxidant capacity were selected to further investigate their interactions by response surface methodology. The optimal extraction parameters of natural antioxidants from Akebia trifoliata peels were ethanol concentration, 49.61% (v/v); solvent-to-material ratio, 32.59:1 mL/g; extraction time, 39.31 min; microwave power, 500 W; and extraction temperature, 50 °C. Under optimal conditions, the FRAP, TEAC and TPC values of Akebia trifoliata peel extracts were 351.86 ± 9.47 µM Fe(II)/g dry weight (DW), 191.12 ± 3.53 µM Trolox/g DW and 32.67 ± 0.90 mg gallic acid equivalent (GAE)/g DW, respectively. Furthermore, the main bioactive compounds (chlorogenic acid, rutin and ellagic acid) in the extract were determined by high-performance liquid chromatography. The results are useful for the full utilization of the by-product from Akebia trifoliate fruit.

scholarly journals Response Surface Optimization for Antioxidant Extraction and Attributes Liking from Roasted Rice Germ Flavored Herbal Tea

Processes ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 125
Author(s):  
Sriwiang Rittisak ◽  
Ratchanee Charoen ◽  
Natthaya Choosuk ◽  
Wanticha Savedboworn ◽  
Wiboon Riansa-ngawong
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Antioxidant Potential ◽  
Optimal Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Polyphenol Content ◽  
Herbal Tea ◽  
Total Polyphenol ◽  
Total Polyphenol Content

The optimal process conditions when examining the antioxidant potential, total polyphenol content, and attribute liking in roasted rice germ flavored herbal tea were investigated using response surface methodology (RSM). The influence upon the extraction process of time and temperature was assessed using a full factorial design on three levels with two variables (32), involving five central point replicates. Extraction temperature (70 °C, 80 °C, and 90 °C) and extraction time (3 min, 4.5 min, and 6 min) served as independent variables, while the dependent variables were allocated to the regression equation to determine antioxidant activity (R2 = 0.941) along with total polyphenol content (R2 = 0.849), flavor liking score (R2 = 0.758), and overall liking score (R2 = 0.816). Following experimentation, it was determined that the optimal time and temperature conditions to maximize total polyphenol content, antioxidant activity, flavor, and overall liking score were in a range of 86 °C to 90 °C for 3.4 min to 5.9 min. When these conditions were imposed, the antioxidant potential, total polyphenol content, flavor, and overall liking score were >70% for DPPH scavenging activity, >75 mgGAE/g, >6.7 (like moderately), and >6.5 (like moderately), respectively.

scholarly journals The optimization of a conventional extraction of bioactive compounds from Cornus mas by RSM and the determination of favourability factors by GIS technique

2021 ◽  
Vol 49 (2) ◽  
pp. 12307
Author(s):  
Iuliana-Maria ENACHE ◽  
Gigi COMAN ◽  
Sanda ROȘCA ◽  
Camelia VIZIREANU ◽  
Liliana MIHALCEA
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Ethanol Concentration ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Extraction Temperature ◽  
Solvent Concentration ◽  
Relevant Variable ◽  
Cornus Mas ◽  
Gis Technique

Rich in vitamins, polyphenols, carotenoids, carbohydrates, with a high antioxidant activity, cornelian cherry (Cornus mas) is one of the 65 species of Cornaceae family, fruits that are used in food industry, cosmetic industry or homeopathic medicine. The valuable bioactive compounds and the high economical potential of this tree makes suitable to be considered as a raw material to study the optimum extraction conditions in order to maximize the bioactive compounds’ concentration. In this study, the main purpose was to investigate both the favourable and restrictive conditions for Cornus mas using the GIS technique. The Remote Sensing and Geographic Information Systems (GIS) technique indicated the favourability factors for Cornus mas trees in Romania country. The response surface methodology was employed to study the effect of temperature, time and solvent concentration on the flavonoids’ (TFC) and polyphenols (TPC) concentrations and the antioxidant activity values. The maximum concentration of TPC (24.70 mg GAE/g raw material) and TFC (1.36 mg QE/g raw material) were obtained with 60% ethanol concentration at different temperature and time values. The cube model with centred faces for the Box-Behnken central composition revealed the highest value of the concentration of TAA (101.31 mg TE/g raw material) at 100% ethanol concentration, 50 °C extraction temperature and the maximum extraction time of 30 min. The most relevant variable was the solvent concentration and the obtained results suggested that the central-face cube model (86% for TPC, 97% for TFC and 95% antioxidant activity) approximated the experimental data.

Optimization of preparation of rosehip tea from the area of Stara Planina Mt

Etnobotanika ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 57-69
Author(s):  
Danijela Kostić ◽  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Therapeutic Effects ◽  
Bioactive Substances ◽  
Rosa Canina ◽  
Maximum Extraction ◽  
High Antioxidant Activity ◽  
Medicinal Properties

Stara Planina Mt is rich in medicinal plants, that are natural sources of various bioactive substances and have many useful medicinal properties. Rosehip, the fruit of wild rose (Rosa canina L.) is very popular and widely used. There is extensive empirical evidence of its therapeutic effects. The high content of vitamins, organic acids, phenolic compounds, carotenoids and pleasant taste define it as a valuable dietary supplement. There are different procedures for making rosehip teas. In this paper, we have optimized the process of preparation of rosehip tea from the aspect of maximum extraction and preservation of vitamin C content in tea. The antioxidant activity of aqueous extracts of rosehips, depending on the extraction time and the temperature at which the extraction is performed, was also examined..The free radical scavenging activity of the plant extracts was analyzed by using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. Optimal conditions are 30 minutes and a temperature of 40 °С. In this way, vitamin C is best extracted and its stability is preserved, which results in the high antioxidant activity of the tested teas.

scholarly journals Ultrasonication assisted extraction of total flavonoids from Kaempferia galanga L. and its antioxidant activity

2020 ◽  
Vol 49 (3) ◽  
pp. 601-609
Author(s):  
Zhaobao Xiang ◽  
Xiaoling Wu ◽  
Xue Zhong
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Optimum Conditions ◽  
Validation Test ◽  
Kaempferia Galanga ◽  
Assisted Extraction

Ultrasonication assisted extraction (UAE) was employed to extract total flavonoids (TF) from Kaempferia galanga L. (KGL) roots. Single-factor experiments were performed to evaluate the effects of the process parameters (solvent-to-material ratio, ethanol concentration, extraction time, ultrasonic power, and extraction temperature) on the TF yield and the response surface methodology (RSM) was used to optimize the extraction parameters. The optimum conditions to obtain the maximum TF yield were solvent-to-material ratio, 25; ethanol concentration, 62%; extraction time, 27.0 min; extraction temperature, 50℃ and ultrasonic power, 225 W. The yield of TF under these conditions reached 3.22 ± 0.12 mg/g of dry root. The analysis of variance and validation test indicated that the proposed method is useful and reliable. The antioxidant capacities of TF of KGL was determined by DPPH· and ABTS+· methods, which revealed that it has good antioxidant activity in both systems.

scholarly journals Antioxidant Capacity and Total Phenolic and Flavonoid Contents of Methanolic Extracts of Urtica dioica L. by Different Extraction Techniques

2020 ◽  
pp. 207-214
Author(s):  
Sabina Begić ◽  
Emir Horozić ◽  
Hurija Alibašić ◽  
Edita Bjelić ◽  
Salih Seferović ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Soxhlet Extraction ◽  
Biologically Active ◽  
Total Phenolic ◽  
Bioactive Components ◽  
Extraction Techniques ◽  
Active Components ◽  
Methanolic Extracts ◽  
High Antioxidant Activity

In this study, the efficacy of different extraction techniques (maceration, ultrasound-assisted and Soxhlet extraction) on the content of biologically active components in extracts from fresh and dried nettle leaves, and their antioxidant activity were analyzed. Methanol was used as the solvent. Total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu, DPPH and FRAP methods, respectively. High content of total phenolic compounds and high antioxidant activity were recorded in extracts of dried nettle. Extracts obtained from fresh nettle samples showed significantly lower content of analyzed bioactive components and lower antioxidant activity. In the case of all extracts, Soxhlet extraction proved to be the most efficient, and maceration the least efficient extraction technique for isolation of bioactive components from nettle leaves.

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