scholarly journals Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs

Geofluids ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yi Gu ◽  
Zhenzhen Wu ◽  
Guan Wang ◽  
Bo Zhi ◽  
Jiliang Yu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Oil Recovery ◽  
High Throughput Sequencing ◽  
Particle Diameter ◽  
Field Trials ◽  
Air Injection ◽  
Oil Reservoirs ◽  
Intermittent Aeration ◽  
Real Time Quantitative Pcr ◽  
Facultative Anaerobic

Oil emulsification is one of the major mechanisms for microbially enhanced oil recovery (MEOR). Although air injection is generally recommended for field trials of MEOR in China, its influence on the microbial community structure in oil reservoirs remains poorly understood, especially activation of emulsifier-producing bacteria. Herein, the effects of air injection on oil emulsification, nutrient consumption, oil properties, and microbial community structures were compared for activated cultures under four different oxygen content conditions: anaerobic, facultative anaerobic, intermittent aeration, and aerobic. The results showed that crude oil in aerobic and intermittent aeration cultures was emulsified effectively when nutrients were thoroughly depleted. The particle diameter of emulsified droplets was 4.74-10.02 μm. High-throughput sequencing results showed that Bacillus and Aeribacillus were effectively activated under aerobic and intermittent aeration conditions, while Tepidimicrobium and Coprothermobacter were activated under facultative anaerobic and anaerobic conditions. Real-time quantitative PCR results showed that the initial emulsifying effect was positively correlated with the abundance of Aeribacillus pallidus.

The Mechanism of Flue Gas Injection for Enhanced Light Oil Recovery

2004 ◽  
Vol 126 (2) ◽  
pp. 119-124 ◽  
Author(s):  
O. S. Shokoya ◽  
S. A. (Raj) Mehta ◽  
R. G. Moore ◽  
B. B. Maini ◽  
M. Pooladi-Darvish ◽  
...  
Keyword(s):  
Oil Recovery ◽  
Flue Gas ◽  
Gas Injection ◽  
Cost Effective ◽  
Air Injection ◽  
Oil Reservoirs ◽  
Flue Gases ◽  
Low Permeability ◽  
Light Oil ◽  
Light Oil Reservoirs

Flue gas injection into light oil reservoirs could be a cost-effective gas displacement method for enhanced oil recovery, especially in low porosity and low permeability reservoirs. The flue gas could be generated in situ as obtained from the spontaneous ignition of oil when air is injected into a high temperature reservoir, or injected directly into the reservoir from some surface source. When operating at high pressures commonly found in deep light oil reservoirs, the flue gas may become miscible or near–miscible with the reservoir oil, thereby displacing it more efficiently than an immiscible gas flood. Some successful high pressure air injection (HPAI) projects have been reported in low permeability and low porosity light oil reservoirs. Spontaneous oil ignition was reported in some of these projects, at least from laboratory experiments; however, the mechanism by which the generated flue gas displaces the oil has not been discussed in clear terms in the literature. An experimental investigation was carried out to study the mechanism by which flue gases displace light oil at a reservoir temperature of 116°C and typical reservoir pressures ranging from 27.63 MPa to 46.06 MPa. The results showed that the flue gases displaced the oil in a forward contacting process resembling a combined vaporizing and condensing multi-contact gas drive mechanism. The flue gases also became near-miscible with the oil at elevated pressures, an indication that high pressure flue gas (or air) injection is a cost-effective process for enhanced recovery of light oils, compared to rich gas or water injection, with the potential of sequestering carbon dioxide, a greenhouse gas.

Risk Assessment and Enhanced Oil Recovery Effect of Gravity Assisted Oxygen-Reduced Air Flooding

2021 ◽  
Author(s):  
Lijuan Huang ◽  
Zongfa Li ◽  
Shaoran Ren ◽  
Yanming Liu
Keyword(s):  
Natural Gas ◽  
Oil Recovery ◽  
Gas Injection ◽  
Oil Production ◽  
Air Injection ◽  
Oil Reservoirs ◽  
Displacement Efficiency ◽  
Risk Area ◽  
Recovery Method ◽  
High Risk Area

Abstract The technology of air injection has been widely used in the second and tertiary recovery in oilfields. However, due to the injected air and natural gas will explode, the safety of the gas injection technology has attracted much attention. Gravity assisted oxygen-reduced air flooding is a new method that eliminates explosion risks and improves oil recovery in large-dip oil reservoirs or thick oil layers. The explosion limit data of different components of natural gas under high pressure were obtained through explosion experiments, which verified the suppression effect of oxygen-reduced air on explosions. The influence of natural gas composition and concentration on explosion limits was also investigated. In addition, a rotatable displacement device was used to study the feasibility of gravity assisted oxygen-reduced air injection for improving the heavy oil reservoirs recovery. Under pressure and temperature conditions of 20MPa and 371K, the sand-filled gravity flooding experiments with different dip angles were carried out using oxygen-reduced air with an oxygen content of 8%. The results show that with the increase of the reservoir dip, the pore volume of the injected fluid at the gas channeling point, the efficient development time of gas injection, and the final displacement efficiency of gas injection development all increase through gravity stabilization caused by gravity differentiation. In the presence of a dip angle, the cumulative oil production before the gas breakthrough point exceeded 80% of the oil production during the entire production process, indicating that gravity assisted oxygen-reduced air flooding is an effective and safe improving oil recovery method. Finally, the explosion risk of each link of the air injection process is analyzed, and the high-risk area and the low-risk area are determined.

scholarly journals INVESTIGATION OF AIR INJECTION TO ENHANCED OIL RECOVERY FROM MEDIUM OIL RESERVOIR OF UPPER INDUS BASIN OF PAKISTAN

2021 ◽  
Vol 9 (12) ◽  
pp. 26-38
Author(s):  
Allah Bakhsh ◽  
Liang Zhang ◽  
Azizullah Shaikh ◽  
Ren Shaoran ◽  
Syed Jamaluddin ◽  
...  
Keyword(s):  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Low Cost ◽  
Reaction Rates ◽  
Air Injection ◽  
Oil Reservoirs ◽  
Indus Basin ◽  
Laboratory Research ◽  
Moderate Pressure ◽  
Original Oil In Place

Previously, air injection is exclusively used in light oil reservoirs; however, laboratory research has shown that air injection can also be very efficient for medium and heavy oil recovery. Due to the low cost of air injection and its indefinite availability, it has an economic advantage over other Enhanced Oil Recovery methods. This study is carried out in an experiment conducted on air injection into medium oil reservoirs. To better understand the air injection procedure for enhancing oil recovery from the X field's medium oil (26.12 °API) of Pakistan reservoir, 14 runs were performed. The effects of air flux, porous media, temperature, and pressure on oxidation reaction rates were explored and measured. The consumption of oxygen at a rate of 90% was determined. At a moderate pressure of 7300 kPa, a significant oil recovery of around 81% of the original oil in place was observed. Increased air flux and low permeability can have a more significant effect on medium oil recovery. The technique produced flue gases that were exceptionally low in carbon oxides, with a typical gas composition of 12% CO2, 6% CO, and unreacted oxygen. This research will contribute to a better knowledge of the air injection method and allow for the optimum performance for a specified reservoir. In the Enhanced oil recovery, a less costly process using this method will be inspiring due to recovering oil in this region.

scholarly journals Effect of Ag Nanoparticles on Denitrification and Microbial Community in a Paddy Soil

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Zhang ◽  
Di Dang ◽  
Lingsi Zheng ◽  
Lingyu Wu ◽  
Yu Wu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Real Time ◽  
Quantitative Pcr ◽  
High Throughput ◽  
Paddy Soil ◽  
Ag Nanoparticles ◽  
High Throughput Sequencing ◽  
Emission Rate ◽  
Rrna Gene ◽  
Real Time Quantitative Pcr

The extensive application of Ag nanoparticles (AgNPs) in industry, agriculture, and food processing areas increases the possibility of its release and accumulation to agroecosystem, but the effects of AgNPs to denitrification and the microbial community in paddy ecosystems are still poorly studied. In this study, microcosmic simulation experiments were established to investigate the response of soil denitrification to different levels of AgNPs (i.e., 0.1, 1, 10, and 50 mg/kg) in a paddy soil. Real-time quantitative PCR and high-throughput sequencing were conducted to reveal the microbial mechanism of the nanometer effect. The results showed that, though 0.1–10 mg/kg AgNPs had no significant effects on denitrification rate and N2O emission rate compared to CK and bulk Ag treatments, 50 mg/kg AgNPs significantly stimulated more than 60% increase of denitrification rate and N2O emission rate on the 3rd day (P < 0.05). Real-time quantitative PCR revealed that 50 mg/kg AgNPs significantly decreased the abundance of 16S bacterial rRNA gene, nirS/nirK, cnorB, and nosZ genes, but it did not change the narG gene abundance. The correlation analysis further revealed that the cumulative N2O emission was positively correlated with the ratio of all the five tested denitrifying genes to bacterial 16S rRNA gene (P < 0.05), indicating that the tolerance of narG gene to AgNPs was the key factor of the increase in denitrification in the studied soil. High-throughput sequencing showed that only the 50-mg/kg-AgNP treatment significantly changed the microbial community composition compared to bulk Ag and CK treatments. The response of microbial phylotypes to AgNPs suggested that the most critical bacteria which drove the stimulation of 50 mg/kg AgNPs on N2O emission were Firmicutes and β-proteobacteria, such as Clotridiales, Burkholderiales, and Anaerolineales. This study revealed the effects of AgNPs to denitrification in a paddy ecosystem and could provide a scientific basis for understanding of the environmental and toxicological effects of Ag nanomaterials.

scholarly journals Bioemulsification and Microbial Community Reconstruction in Thermally Processed Crude Oil

2021 ◽  
Vol 9 (10) ◽  
pp. 2054
Author(s):  
Bing Hu ◽  
Jie-Yu Zhao ◽  
Yong Nie ◽  
Xiao-Yu Qin ◽  
Kai-Duan Zhang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Oil Recovery ◽  
Low Cost ◽  
Thermal Recovery ◽  
Oil Reservoirs ◽  
Deep Biosphere ◽  
Degrading Bacteria ◽  
Thermally Processed ◽  
Mesophilic Conditions

Utilization of low-cost, environmental-friendly microbial enhanced oil recovery (MEOR) techniques in thermal recovery-processed oil reservoirs is potentially feasible. However, how exogenous microbes facilitate crude oil recovery in this deep biosphere, especially under mesophilic conditions, is scarcely investigated. In this study, a thermal treatment and a thermal recurrence were processed on crude oil collected from Daqing Oilfield, and then a 30-day incubation of the pretreated crude oil at 37 °C was operated with the addition of two locally isolated hydrocarbon-degrading bacteria, Amycolicicoccus subflavus DQS3-9A1T and Dietzia sp. DQ12-45-1b, respectively. The pH, surface tension, hydrocarbon profiles, culture-dependent cell densities and taxonomies, and whole and active microbial community compositions were determined. It was found that both A. subflavus DQS3-9A1T and Dietzia sp. DQ12-45-1b successfully induced culture acidification, crude oil bioemulsification, and residual oil sub-fraction alteration, no matter whether the crude oil was thermally pretreated or not. Endogenous bacteria which could proliferate on double heated crude oil were very few. Compared with A. subflavus, Dietzia sp. was substantially more effective at inducing the proliferation of varied species in one-time heated crude oil. Meanwhile, the effects of Dietzia sp. on crude oil bioemulsification and hydrocarbon profile alteration were not significantly influenced by the ploidy increasing of NaCl contents (from 5 g/L to 50 g/L), but the reconstructed bacterial communities became very simple, in which the Dietzia genus was predominant. Our study provides useful information to understand MEOR trials on thermally processed oil reservoirs, and proves that this strategy could be operated by using the locally available hydrocarbon-degrading microbes in mesophilic conditions with different salinity degrees.

The Mechanism of Flue Gas Injection for Enhanced Light Oil Recovery

2002 ◽  
Author(s):  
O. S. Shokoya ◽  
S. A. Mehta ◽  
R. G. Moore ◽  
B. B. Maini ◽  
M. Pooladi-Darvish ◽  
...  
Keyword(s):  
Oil Recovery ◽  
Flue Gas ◽  
Gas Injection ◽  
Cost Effective ◽  
Air Injection ◽  
Oil Reservoirs ◽  
Flue Gases ◽  
Low Permeability ◽  
Light Oil ◽  
Light Oil Reservoirs

Flue gas injection into light oil reservoirs could be a cost-effective gas displacement method for enhanced oil recovery, especially in low porosity and low permeability reservoirs. The flue gas could be generated in situ as obtained from the spontaneous ignition of oil when air is injected into a high temp erature reservoir, or injected directly into the reservoir from some surface source. When operating at high pressures commonly found in deep light oil reservoirs, the flue gas may become miscible or near miscible with the reservoir oil, thereby displacing it more efficiently than an immiscible gas flood. Some successful high pressure air injection (HPAI) projects have been reported in low permeability and low porosity light oil reservoirs. Spontaneous oil ignition was reported in these projects, at least from laboratory experiments; however, the mechanism by which the generated flue gas displaces the oil has not been discussed in clear terms in the literature. An experimental investigation was carried out to study the mechanism by which flue gases displace light oil at a reservoir temperature of 116 °C and typical reservoir pressures ranging from 4,028 psi (27.77 MPa) to 6,680 psi (46.06 MPa). The results showed that the flue gases displaced the oil in a forward contacting process resembling a combined vaporizing and condensing multi-contact gas drive mechanism. The flue gases also became near-miscible with the oil at elevated pressures, an indication that high pressure flue gas (or air) injection is a cost-effective process for enhanced recovery of light oils, compared to rich gas or water injection, with the potential of sequestering greenhouse gases.

scholarly journals Disentangling Responses of the Subsurface Microbiome to Wetland Status and Implications for Indicating Ecosystem Functions

2021 ◽  
Vol 9 (2) ◽  
pp. 211
Author(s):  
Jie Gao ◽  
Miao Liu ◽  
Sixue Shi ◽  
Ying Liu ◽  
Yu Duan ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Carbon Fixation ◽  
Microbial Community Composition ◽  
Ecosystem Functions ◽  
Microbial Composition ◽  
Wetland Ecosystems ◽  
Soil Microbial ◽  
Geochemical Properties ◽  
Microbial Groups

In this study, we analyzed microbial community composition and the functional capacities of degraded sites and restored/natural sites in two typical wetlands of Northeast China—the Phragmites marsh and the Carex marsh, respectively. The degradation of these wetlands, caused by grazing or land drainage for irrigation, alters microbial community components and functional structures, in addition to changing the aboveground vegetation and soil geochemical properties. Bacterial and fungal diversity at the degraded sites were significantly lower than those at restored/natural sites, indicating that soil microbial groups were sensitive to disturbances in wetland ecosystems. Further, a combined analysis using high-throughput sequencing and GeoChip arrays showed that the abundance of carbon fixation and degradation, and ~95% genes involved in nitrogen cycling were increased in abundance at grazed Phragmites sites, likely due to the stimulating impact of urine and dung deposition. In contrast, the abundance of genes involved in methane cycling was significantly increased in restored wetlands. Particularly, we found that microbial composition and activity gradually shifts according to the hierarchical marsh sites. Altogether, this study demonstrated that microbial communities as a whole could respond to wetland changes and revealed the functional potential of microbes in regulating biogeochemical cycles.

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