scholarly journals Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Abebe Bizuye ◽  
Lashitew Gedamu ◽  
Christine Bii ◽  
Erastus Gatebe ◽  
Naomi Maina
Keyword(s):  
Silver Nanoparticles ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Functional Groups ◽  
Visual Detection ◽  
Color Change ◽  
Bioactive Metabolites ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Genus Streptomyces

Infectious diseases caused by antibiotic-resistant bacteria lead to a considerable increase in human morbidity and mortality globally. This requires to search potential actinomycete isolates from undiscovered habitats as a source of effective bioactive metabolites and to synthesis metabolite-mediated antibacterial silver nanoparticles (AgNPs). The main purpose of the present study was to identify actinomycetes isolated from Thika waste dump soils that produce bioactive metabolites to synthesize antibacterial AgNPs. The synthesis of metabolite-mediated AgNP was confirmed with visual detection and a UV-vis spectrophotometer, whereas the functional groups involved in AgNP synthesis were identified using a FTIR spectrophotometer. The antibacterial activity of the metabolite-mediated AgNPs was tested by a well diffusion assay. Identification of actinomycete isolates involved in the synthesis of antibacterial AgNPs was done based on 16S rRNA gene sequence analysis. The visual detection showed that dark salmon and pale golden color change was observed due to the formation of AgNPs by KDT32 and KGT32 metabolites, respectively. The synthesis was confirmed by a characteristic UV spectra peak at 415.5 nm for KDT32-AgNP and 416 nm for KGT32-AgNP. The FTIR spectra revealed that OH, C=C, and S-S functional groups were involved in the synthesis of KDT32-AgNP, whereas OH, C=C, and C-H were involved in the formation of KGT32-AgNP. The inhibition zone results revealed that KDT32-AgNP showed 22.0 ± 1.4 mm and 19.0 ± 1.4 mm against Escherichia coli and Salmonella typhi, whereas KGT32-AgNP showed 21.5 ± 0.7 mm and 17.0 ± 0.0 mm, respectively. KDT32 and KGT32 isolates were identified as genus Streptomyces and their 16S rRNA gene sequences were deposited in the GenBank database with MH301089 and MH301090 accession numbers, respectively. Due to the bactericidal activity of synthesized AgNPs, KDT32 and KGT32 isolates can be used in biomedical applications.

Streptomyces rhizophilus Causes Potato Common Scab Disease

Plant Disease ◽  
2021 ◽  
Author(s):  
Qi Wei ◽  
Jie Li ◽  
Shuai Yang ◽  
Wenzhong Wang ◽  
Fanxiang Min ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Common Scab ◽  
Bacterial Species ◽  
Economic Losses ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Heilongjiang Province ◽  
Epidemic Disease ◽  
Genus Streptomyces

Common scab (CS) caused by Streptomyces spp. is a significant soilborne potato disease that results in tremendous economic losses globally. Identification of CS-associated species of the genus Streptomyces can enhance understanding of the genetic variation of these bacterial species and is necessary for the control of this epidemic disease. The present study isolated Streptomyces strain 6-2-1(1) from scabby potatoes in Keshan County, Heilongjiang Province, China. PCR analysis confirmed that the strain harbored the characteristic Streptomyces pathogenicity island (PAI) genes (txtA, txtAB, nec1, and tomA). Pathogenicity assays proved that the strain caused typical scab lesions on potato tuber surfaces and necrosis on radish seedlings and potato slices. Subsequently, the strain was systemically characterized at morphological, physiological, biochemical and phylogenetic levels. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6-2-1(1) shared 99.86% sequence similarity with Streptomyces rhizophilus JR-41T, isolated initially from bamboo in rhizospheric soil in Korea. PCR amplification followed by Sanger sequencing of the 16S rRNA gene of 164 scabby potato samples collected in Heilongjiang Province from 2019 to 2020 demonstrated that approximately 2% of the tested samples were infected with S. rhizophilus. Taken together, these results demonstrate that S. rhizophilus is capable of causing potato CS disease and may pose a potential challenge to potato production in Heilongjiang Province of China.

scholarly journals Cultivation of Hard-To-Culture Subsurface Mercury-Resistant Bacteria and Discovery of New merA Gene Sequences

2008 ◽  
Vol 74 (12) ◽  
pp. 3795-3803 ◽  
Author(s):  
L. D. Rasmussen ◽  
C. Zawadsky ◽  
S. J. Binnerup ◽  
G. Øregaard ◽  
S. J. Sørensen ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Techniques ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Direct Plating ◽  
Mercury Reduction ◽  
Mercury Resistant Bacteria

ABSTRACT Mercury-resistant bacteria may be important players in mercury biogeochemistry. To assess the potential for mercury reduction by two subsurface microbial communities, resistant subpopulations and their merA genes were characterized by a combined molecular and cultivation-dependent approach. The cultivation method simulated natural conditions by using polycarbonate membranes as a growth support and a nonsterile soil slurry as a culture medium. Resistant bacteria were pregrown to microcolony-forming units (mCFU) before being plated on standard medium. Compared to direct plating, culturability was increased up to 2,800 times and numbers of mCFU were similar to the total number of mercury-resistant bacteria in the soils. Denaturing gradient gel electrophoresis analysis of DNA extracted from membranes suggested stimulation of growth of hard-to-culture bacteria during the preincubation. A total of 25 different 16S rRNA gene sequences were observed, including Alpha-, Beta-, and Gammaproteobacteria; Actinobacteria; Firmicutes; and Bacteroidetes. The diversity of isolates obtained by direct plating included eight different 16S rRNA gene sequences (Alpha- and Betaproteobacteria and Actinobacteria). Partial sequencing of merA of selected isolates led to the discovery of new merA sequences. With phylum-specific merA primers, PCR products were obtained for Alpha- and Betaproteobacteria and Actinobacteria but not for Bacteroidetes and Firmicutes. The similarity to known sequences ranged between 89 and 95%. One of the sequences did not result in a match in the BLAST search. The results illustrate the power of integrating advanced cultivation methodology with molecular techniques for the characterization of the diversity of mercury-resistant populations and assessing the potential for mercury reduction in contaminated environments.

scholarly journals The Household Resistome: Frequency of β-Lactamases, Class 1 Integrons, and Antibiotic-Resistant Bacteria in the Domestic Environment and Their Reduction during Automated Dishwashing and Laundering

2020 ◽  
Vol 86 (23) ◽  
Author(s):  
Laura Schages ◽  
Ralf Lucassen ◽  
Florian Wichern ◽  
Rainer Kalscheuer ◽  
Dirk Bockmühl
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Domestic Environment ◽  
Private Households ◽  
Washing Machines ◽  
Gene Copies

ABSTRACT Households provide a habitat for bacteria originating from humans, animals, foods, contaminated clothes, or other sources. Thus, bacteria carrying antibiotic resistance genes (ARGs) may be introduced via household members, animals, or the water supply from external habitats into private households and vice versa. Since data on antibiotic resistance (ABR) in the domestic environment are limited, this study aimed to determine the abundance of β-lactamase, mobile colistin resistance, and class 1 integron genes and the correlation of their presence and to characterize phenotypically resistant strains in 54 private households in Germany. Additionally, the persistence of antibiotic-resistant bacteria during automated dishwashing compared to that during laundering was assessed. Shower drains, washing machines, and dishwashers were sampled and analyzed using quantitative real-time PCR. Resistant strains were isolated, followed by identification and antibiotic susceptibility testing using a Vitek 2 system. The results showed a significantly higher relative ARG abundance of 0.2367 ARG copies/16S rRNA gene copies in shower drains than in dishwashers (0.1329 ARG copies/16S rRNA gene copies) and washing machines (0.0006 ARG copies/16S rRNA gene copies). blaCMY-2, blaACT/MIR, and blaOXA-48 were the most prevalent ARG, and intI1 occurred in 96.3% of the households, while no mcr genes were detected. Several β-lactamase genes co-occurred, and the resistance of bacterial isolates correlated positively with genotypic resistance, with carbapenemase genes dominating across isolates. Antibiotic-resistant bacteria were significantly reduced during automated dishwashing as well as laundering tests and did not differ from susceptible strains. Overall, the domestic environment may represent a potential reservoir of β-lactamase genes and β-lactam-resistant bacteria, with shower drains being the dominant source of ABR. IMPORTANCE The abundance of antibiotic-resistant bacteria and ARGs is steadily increasing and has been comprehensively analyzed in natural environments, animals, foods, and wastewater treatment plants. In this respect, β-lactams and colistin are of particular interest due to the emergence of multidrug-resistant Gram-negative bacteria. Despite the connection of private households to these environments, only a few studies have focused on the domestic environment so far. Therefore, the present study further investigated the occurrence of ARGs and antibiotic-resistant bacteria in shower drains, washing machines, and dishwashers. The analysis of the domestic environment as a potential reservoir of resistant bacteria is crucial to determine whether households contribute to the spread of ABR or may be a habitat where resistant bacteria from the natural environment, humans, food, or water are selected due to the use of detergents, antimicrobial products, and antibiotics. Furthermore, ABR could limit the options for the treatment of infections arising in the domestic environment.

scholarly journals Multilocus sequence analysis of phytopathogenic species of the genus Streptomyces

2011 ◽  
Vol 61 (10) ◽  
pp. 2525-2531 ◽  
Author(s):  
David P. Labeda
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Multilocus Sequence Analysis ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Genus Streptomyces ◽  
Type Strains

The identification and classification of species within the genus Streptomyces is difficult because there are presently 576 species with validly published names and this number increases every year. The value of multilocus sequence analysis applied to the systematics of Streptomyces species has been well demonstrated in several recently published papers. In this study the sequence fragments of four housekeeping genes, atpD, recA, rpoB and trpB, were determined for the type strains of 10 known phytopathogenic species of the genus Streptomyces, including Streptomyces scabiei, Streptomyces acidiscabies, Streptomyces europaeiscabiei, Streptomyces luridiscabiei, Streptomyces niveiscabiei, Streptomyces puniciscabiei, Streptomyces reticuliscabiei, Streptomyces stelliscabiei, Streptomyces turgidiscabies and Streptomyces ipomoeae, as well as six uncharacterized phytopathogenic Streptomyces isolates. The type strains of 52 other species, including 19 species observed to be phylogenetically closely related to these, based on 16S rRNA gene sequence analysis, were also included in the study. Phylogenetic analysis of single gene alignments and a concatenated four-gene alignment demonstrated that the phytopathogenic species are taxonomically distinct from each other in spite of high 16S rRNA gene sequence similarities and provided a tool for the identification of unknown putative phytopathogenic Streptomyces strains at the species level.

scholarly journals Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

2016 ◽  
Vol 65 (3) ◽  
pp. 319-329 ◽  
Author(s):  
Ajit K. Passari ◽  
Vineet K. Mishra ◽  
Vijai K. Gupta ◽  
Ratul Saikia ◽  
Bhim P. Singh
Keyword(s):  
Medicinal Plants ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Fungal Pathogens ◽  
Biocontrol Agents ◽  
Rrna Gene ◽  
Streptomyces Species ◽  
Genus Streptomyces ◽  
Fungal Phytopathogens ◽  
Endophytic Streptomyces

The prospective of endophytic microorganisms allied with medicinal plants is disproportionally large compared to those in other biomes. The use of antagonistic microorganisms to control devastating fungal pathogens is an attractive and eco-friendly substitute for chemical pesticides. Many species of actinomycetes, especially the genus Streptomyces, are well known as biocontrol agents. We investigated the culturable community composition and biological control ability of endophytic Streptomyces sp. associated with an ethanobotanical plant Schima wallichi. A total of 22 actinobacterial strains were isolated from different organs of selected medicinal plants and screened for their biocontrol ability against seven fungal phytopathogens. Seven isolates showed significant inhibition activity against most of the selected pathogens. Their identification based on 16S rRNA gene sequence analysis, strongly indicated that all strains belonged to the genus Streptomyces. An endophytic strain BPSAC70 isolated from root tissues showed highest percentage of inhibition (98.3 %) against Fusarium culmorum with significant activity against other tested fungal pathogens. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all seven strains shared 100 % similarity with the genus Streptomyces. In addition, the isolates were subjected to the amplification of antimicrobial genes encoding polyketide synthase type I (PKS-I) and nonribosomal peptide synthetase (NRPS) and found to be present in most of the potent strains. Our results identified some potential endophytic Streptomyces species having antagonistic activity against multiple fungal phytopathogens that could be used as an effective biocontrol agent against pathogenic fungi.

scholarly journals Isolasi dan Identifikasi Bakteri Resisten Merkuri melalui Analisis Gen 16S rRNA pada Urin Pasien dengan Tumpatan Amalgam

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Eunike M. Karamoy ◽  
. . Fatimawali ◽  
Billy J. Kepel
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Human Body ◽  
Dental Amalgam ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Bacillus Sp ◽  
Mercury Resistant Bacteria

Abstract: Mercury is a hazardous metal used as the main component of amalgam. Amalgam is a source of the chronic exposure of elemental mercury (Hg0) to human body. In the body, mercury is excreted through urine. Chronic exposure to mercury causes bacteria to develop resistance using mer operon. By analyzing the 16S rRNA gene, the bacterial identification is more specific and able to facilitate further studies about mercury-resistant bacteria within the human body. This study was aimed to identify the mercury-resistant bacteria isolated from the urine of patients with dental amalgam. This was an explorative descriptive study by isolation and identification of mercury-resistant bacteria in the urine samples of patients with dental amalgam and studying the evolutionary relationship through 16S rRNA gene. The nucleotide sequence of 16S rRNA gene of bacteria from urine samples of patients with dental amalgam showed 100% resemblances with Bacillus cereus. The phylogenetic tree showed that Bacillus cereus was closely related to Bacillus anthracis, Bacillus sp., and Bacillus thuringiensis. Conclusion: Mercury-resistant bacteria isolated from urine of patients with dental amalgam was Bacillus cereus, which was not the normal flora of human body. Bacillus cereus had close evolutionary relationship with Bacillus anthracis, Bacillus sp., and Bacillus thuringiensis.Keywords: mercury-resistant bacteria, 16S rRNA gene, urine, amalgam. Abstrak: Merkuri adalah logam berbahaya komponen utama pada amalgam. Amalgam merupakan sumber paparan kronis merkuri elemental (Hg0) pada manusia. Di dalam tubuh, merkuri diekskresikan melalui urin. Akibat terpapar merkuri dalam waktu lama, bakteri dalam urin mengembangkan mekanisme resistensi terhadap merkuri melalui operon mer. Melalui analisis gen 16S rRNA, identifikasi bakteri lebih spesifik sehingga mempermudah untuk mempelajari dan meneliti lebih lanjut tentang bakteri resisten merkuri pada tubuh manusia. Penelitian ini bertujuan untuk mengetahui jenis bakteri resisten merkuri yang diisolasi dari urin pasien dengan tumpatan amalgam. Jenis penelitian ialah deskriptif eksploratif, melalui isolasi dan identifikasi bakteri resisten merkuri dari urin pasien pengguna amalgam serta melihat hubungan kekerabatan bakteri tersebut melalui gen 16S rRNA. Hasil penelitian mendapatkan urutan nukleotida gen 16S rRNA bakteri dari urin pasien pengguna amalgam memiliki kesamaan 100% paling banyak dengan Bacillus cereus. Berdasarkan analisis hubungan kekerabatan, Bacillus cereus berkerabat dekat dengan Bacillus anthracis, Bacillus sp, dan Bacillus thuringiensis. Simpulan: Bakteri resisten merkuri yang diisolasi dari sampel urin pasien pengguna amalgam ialah Bacillus cereus, yang bukan merupakan flora normal tubuh manusia. Bacillus cereus memiliki hubungan kekerabatan dekat dengan Bacillus anthracis, Bacillus sp, dan Bacillus thuringiensis.Kata kunci: bakteri resisten merkuri, gen 16S rRNA, urin, amalgam

scholarly journals Characterization of Streptomyces sporangiiformans sp. nov., a Novel Soil Actinomycete with Antibacterial Activity against Ralstonia solanacearum

2019 ◽  
Vol 7 (9) ◽  
pp. 360 ◽  
Author(s):  
Junwei Zhao ◽  
Liyuan Han ◽  
Mingying Yu ◽  
Peng Cao ◽  
Dongmei Li ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Ralstonia Solanacearum ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Phytopathogenic Bacterium ◽  
Genus Streptomyces

Ralstonia solanacearum is a major phytopathogenic bacterium that attacks many crops and other plants around the world. In this study, a novel actinomycete, designated strain NEAU-SSA 1T, which exhibited antibacterial activity against Ralstonia solanacearum, was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of the genus Streptomyces. The 16S rRNA gene sequence analysis showed that the isolate was most closely related to Streptomyces aureoverticillatus JCM 4347T (97.9%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a cluster with Streptomyces vastus JCM4524T (97.4%), S. cinereus DSM43033T (97.2%), S. xiangluensis NEAU-LA29T (97.1%) and S. flaveus JCM3035T (97.1%). The cell wall contained LL-diaminopimelic acid and the whole-cell hydrolysates were ribose, mannose and galactose. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), hydroxy-phosphatidylethanolamine (OH-PE), phosphatidylinositol (PI), two phosphatidylinositol mannosides (PIMs) and an unidentified phospholipid (PL). The menaquinones were MK-9(H4), MK-9(H6), and MK-9(H8). The major fatty acids were iso-C17:0, C16:0 and C17:1 ω9c. The DNA G+C content was 69.9 mol %. However, multilocus sequence analysis (MLSA) based on five other house-keeping genes (atpD, gyrB, recA, rpoB, and trpB), DNA–DNA relatedness, and physiological and biochemical data showed that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-SSA 1T should be classified as representatives of a novel species of the genus Streptomyces, for which the name Streptomyces sporangiiformans sp. nov. is proposed. The type strain is NEAU-SSA 1T (=CCTCC AA 2017028T = DSM 105692T).

scholarly journals First Report Regarding Potato Scab Caused by Streptomyces acidiscabies in Uruguay

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 1064-1064 ◽  
Author(s):  
M. I. Lapaz ◽  
E. Verdier ◽  
M. J. Pianzzola
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Potato Scab ◽  
Rrna Gene ◽  
Genus Streptomyces ◽  
Pathogenicity Genes ◽  
First Report ◽  
Streptomyces Acidiscabies ◽  
Potato Scab Disease ◽  
The 16S Rrna Gene

Potato scab disease is caused by gram-positive filamentous bacteria in the genus Streptomyces. A great variety of species cause this disease, but Streptomyces scabies is the most ancient of these pathogens and can be found in a worldwide distribution, whereas S. turgidiscabies and S. acidiscabies are newly emerged pathogens (2). During the autumn of 2010, potato (Solanum tuberosum) crops had large economic losses by common scab, corresponding to 29% of the total potato-cultivation area (according to our survey), which was unusual in Uruguay. Specifically, the disease was very aggressive and the tubers showed particularly deep scab lesions. We isolated the Streptomyces species present in these particular scab lesions of tubers collected in July 2010 from one of the three potato cultivation areas (San José). A total of 19 Streptomyces spp. strains were isolated and identified using classical and molecular techniques. Morphological characteristics of colonies and microscopic structure of the mycelium were observed (1). Molecular characterization by conventional PCR was carried out using primers directed to specific regions of the 16S rRNA gene for the genus Streptomyces, Aci1: (5′-TCACTCCTGCCTGCATGGGCG-3′) and Aci2: (5′-CGACAGCTCCCTCCCACAAG-3′). Also, regions of two pathogenicity genes, namely txtAB and nec1, were amplified and confirmed by sequencing (2). Additionally, melanin production and pathogenicity of the isolates was determined by inoculation of potato discs (1). Six of the 19 strains succeeded in PCR amplification with primers specific to Streptomyces acidiscabies, which has white, aerial hypha and flexuous spore chains. These strains did not produce melanin on tyrosine agar media. The amplified fragments for 16S rRNA and pathogenicity genes from one representative strain 61 were sequenced. BLASTn analysis of the 16S rRNA gene sequence obtained of the strain 61 (Accession No. JN206667) showed the highest similarity (100%) with S. acidiscabies type strain 84-01-182 (GenBank Accession No. FJ007427.1). Pathogenicity of the isolate was tested on tuber slices. The isolate was grown on YME for 5 to 7 days at 28°C and agar plugs from the sporulating colonies were inverted onto excised tuber tissue. Disks were incubated at 28°C in the dark and the presence of necrosis was evaluated after 5 days (1). All tuber slice assays were repeated three times. The noninoculated control tuber slices did not show any necrosis, while those inoculated with the strain did. To our knowledge, this is the first report of S. acidiscabies causing potato scab disease in Uruguay. References: (1) D. H. Park et al. Plant Dis. 87:1290, 2003. (2) L. A. Wanner. Phytopathology 96:1361, 2006.

scholarly journals Antibacterial Activity of Streptomyces SAE4034 Isolated from Segara Anakan Mangrove Rhizosphere against Antibiotic Resistant Bacteria

2018 ◽  
Vol 10 (1) ◽  
pp. 117-124
Author(s):  
Dini Ryandini ◽  
Hendro Pramono ◽  
Sukanto Sukanto
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Antibacterial Properties ◽  
Bioactive Compound ◽  
Biochemical Properties ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Antibiotic Resistant ◽  
Antibacterial Compound ◽  
Compound Test

Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria

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