scholarly journals Arsenic Accumulation of Realgar Altered by Disruption of Gut Microbiota in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Wenfeng Xu ◽  
Shanshan Zhang ◽  
Wenqing Jiang ◽  
Shuo Xu ◽  
Pengfei Jin
Keyword(s):  
Gut Microbiota ◽  
Whole Blood ◽  
Subcutaneous Injection ◽  
Inductively Coupled Plasma ◽  
High Dose ◽  
Inductively Coupled ◽  
Arsenic Accumulation ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

Objective. To investigate the influence of gut microbiota on arsenic accumulation of realgar in mice. Methods. Mice were treated with antibiotics to form a mouse model of gut microbial disruption. Antibiotic-treated and normally raised mice were given 15 mg/kg, 150 mg/kg, and 750 mg/kg realgar by gavage and 0.2 mg/kg and 1 mg/kg arsenic solution by subcutaneous injection for 7 days. The concentration of arsenic in mice whole blood was determined by inductively coupled plasma mass spectrometry (ICP-MS). Arsenic accumulation in antibiotic-treated mice and normally raised mice was compared. Results. After exposure to low dose (15 mg/kg) and middle dose (150 mg/kg) of realgar, significantly, more arsenic was accumulated in the whole blood of antibiotic-treated mice compared to normally raised counterparts, which indicated that the disruption of gut microbiota could lead to higher arsenic load of realgar in mice. The homeostasis of gut microbiota was supposed to be disrupted by high dose (750 mg/kg) of realgar because after exposure to high dose of realgar, there was no significant difference in arsenic accumulation between antibiotic-treated and normally raised mice. Furthermore, arsenic solution was administered by subcutaneous injection to mice to investigate the influence of gut microbial differences on arsenic accumulation in addition to the absorption process, and there was no significant difference in arsenic accumulation between mice with these two different statuses of gut microbiota. Conclusions. Gut microbiota disruption could increase arsenic accumulation of realgar in mice.

scholarly journals Analysis of whole blood by ICP-MS equipped with a high temperature total sample consumption system

2017 ◽  
Vol 32 (1) ◽  
pp. 78-87 ◽  
Author(s):  
Águeda Cañabate ◽  
Esperanza García-Ruiz ◽  
Martín Resano ◽  
José-Luis Todolí
Keyword(s):  
High Temperature ◽  
Whole Blood ◽  
Inductively Coupled Plasma ◽  
Total Sample ◽  
Sample Introduction ◽  
Element Analysis ◽  
Inductively Coupled ◽  
System P ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

In this work, the performance of a high temperature torch Integrated Sample Introduction System (hTISIS) using inductively coupled plasma mass spectrometry (ICP-MS) for the multi-element analysis of whole blood is evaluated.

scholarly journals Hair Mercury Levels Detection in Fishermen from Sicily (Italy) by ICP-MS Method after Microwave-Assisted Digestion

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Giuseppe Giangrosso ◽  
Gaetano Cammilleri ◽  
Andrea Macaluso ◽  
Antonio Vella ◽  
Nicolantonio D’Orazio ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Total Mercury ◽  
Control Group ◽  
Inductively Coupled ◽  
Hair Mercury ◽  
Icp Ms ◽  
Hair Samples ◽  
Significant Difference ◽  
Close Relationship ◽  
Plasma Mass Spectrometry

A number of ninety-six hair samples from Sicilian fishermen were examined for total mercury detection by an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) method. The mercury levels obtained were compared with mercury levels of 96 hair samples from a control group, in order to assess potential exposure to heavy metals of Sicilian fishermen due to fish consumption and closeness to industrial activities. Furthermore, the mercury levels obtained from hair samples were sorted by sampling area in order to verify the possible risks linked to the different locations. The overall mean concentration in the hair of the population of fishermen was6.45±7.03 μg g−1, with a highest value in a fisherman of Sciacca (16.48 μg g−1). Hair mercury concentration in fishermen group was significantly higher than in control group (p<0.01). There was no significant difference in hair total mercury concentrations between sampling areas (p>0.05). The results of this study indicate a greater risk of exposure to mercury in Sicilian fishermen, in comparison to the control population, due to the high consumption of fish and the close relationship with sources of exposure (ports, dumps, etc.).

scholarly journals Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5370 ◽  
Author(s):  
Wencan Jiang ◽  
Gongwei Sun ◽  
Wenbin Cui ◽  
Shasha Men ◽  
Miao Jing ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Clinical Laboratory ◽  
Clinical Sample ◽  
Measurement Range ◽  
Clinical Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

Background: Element-tagged immunoassay coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection has the potential to revolutionize immunoassay analysis for multiplex detection. However, a further study referring to the standard evaluation and clinical sample verification is needed to ensure its reliability for simultaneous analysis in clinical laboratories. Methods: Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) were chosen for the duplex immunoassay. The performance of the assay was evaluated according to guidelines from the Clinical and Laboratory Standards Institute (CLSI). Moreover, reference intervals (RIs) of CEA and AFP were established. At last, 329 clinical samples were analyzed by the proposed method and results were compared with those obtained with electrochemiluminescent immunoassay (ECLIA) method. Results: The measurement range of the assay was 2–940 ng/mL for CEA and 1.5–1000 ng/mL for AFP, with a detection limit of 0.94 ng/mL and 0.34 ng/mL, respectively. The inter-assay and intra-assay imprecision were all less than 6.58% and 10.62%, respectively. The RI of CEA and AFP was 0–3.84 ng/mL and 0–9.94 ng/mL, respectively. Regarding to clinical sample detection, no significant difference was observed between the proposed duplex assay and the ECLIA method. Conclusions: The ICP-MS-based duplex immunoassay was successfully developed and the analytical performance fully proved clinical applicability. Well, this could be different with other analytes.

scholarly journals Health Risks Assessment of Rainwater Pollutants in Jengka, Pahang, Malaysia

2018 ◽  
Vol 7 (3.30) ◽  
pp. 15
Author(s):  
Siti Nadzifah Ghazali ◽  
Fazrul Razman ◽  
Mohd Zahari Abdullah
Keyword(s):  
Heavy Metals ◽  
Inductively Coupled Plasma ◽  
Principal Component ◽  
Anthropogenic Sources ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Health Risks Assessment ◽  
Risks Assessment

Rainwater samples were collected in Jengka, Pahang Malaysia. Temperature, pH, TDS, EC, Al, Cu, Mn, and Zn were measured. The concentrations of heavy metals (Al, Cu, Mn and Zn) in thirty-eight samples were analysed using inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis results indicate that pH, TDS, and EC showed significant differences between wet and dry seasons. However, no significant difference was observed seasonally for heavy metals (HMs). All parameters, except pH, were below the Malaysian Drinking Water Guidelines (MDWG) value and the health risk assessment for HMs indicates the safe levels. Principal component analysis (PCA) suggests that HMs in rainwater in Jengka, Pahang were originated from natural and anthropogenic sources.  

scholarly journals Rare earth elements in oyster shells: provenance discrimination and potential vital effects

2020 ◽  
Vol 17 (8) ◽  
pp. 2205-2217
Author(s):  
Vincent Mouchi ◽  
Camille Godbillot ◽  
Vianney Forest ◽  
Alexey Ulianov ◽  
Franck Lartaud ◽  
...  
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
Inductively Coupled Plasma ◽  
Hydrothermal Activity ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Vital Effects ◽  
Bivalve Shells

Abstract. Rare earth elements (REEs) and yttrium in seawater originate from atmospheric fallout, continental weathering, and transport from rivers, as well as hydrothermal activity. Previous studies have reported the use of REE and Y measurements in biogenic carbonates as a means to reconstruct these surface processes in ancient times. As coastal seawater REE and Y concentrations partially reflect those of nearby rivers, it may be possible to obtain a regional fingerprint of these concentrations from bivalve shells for seafood traceability and environmental monitoring studies. Here, we present a dataset of 297 measurements of REE and Y abundances by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) from two species (Crassostrea gigas and Ostrea edulis). We measured a total of 49 oyster specimens from six locations in France (Atlantic Ocean and Mediterranean Sea). Our study reports that there is no significant difference in concentrations from shell parts corresponding to winter and summer periods for both species. Moreover, interspecific vital effects are reported from specimens from both species and from the same locality. REE and Y profiles as well as t-distributed stochastic neighbour embedding processing (t-SNE; a discriminant statistical method) indicate that REE and Y measurements from C. gigas shells can be discriminated from one locality to another, but this is not the case for O. edulis, which presents very similar concentrations in all studied localities. Therefore, provenance studies using bivalve shells based on REEs and Y have to first be tested for the species. Other methods have to be investigated to be able to find the provenance of some species, such as O. edulis.

scholarly journals The Interchangeability of Plasma and Whole Blood Metal Ion Measurement in the Monitoring of Metal on Metal Hips

Arthritis ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ibrahim A. Malek ◽  
Joanne Rogers ◽  
Amanda Christina King ◽  
Juliet Clutton ◽  
Daniel Winson ◽  
...  
Keyword(s):  
Whole Blood ◽  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Blood Concentrations ◽  
Inductively Coupled ◽  
Paired Samples ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
The Mean ◽  
High Concentrations

One hundred and twenty six paired samples of plasma and whole blood were measured with inductively coupled plasma mass spectrometry technique for metal ions analysis to determine a relationship between them. There was a significant difference between the mean plasma and whole blood concentrations of both cobalt (Co) and chromium (Cr) (p<0.0001 for both Co and Cr). The mean ratio between plasma and whole blood Cr and Co was 1.56 (range: 0.39–3.85) and 1.54 (range: 0.64–18.26), respectively, but Bland and Altman analysis illustrated that this relationship was not universal throughout the range of concentrations. There was higher variability at high concentrations for both ions. We conclude that both these concentrations should not be used interchangeably and conversion factors are unreliable due to concentration dependent variability.

scholarly journals The Distribution of the Heavy Metals in Soil Sample Along Blue Nile During overflow Season

2017 ◽  
Vol 10 (1) ◽  
pp. 19
Author(s):  
Magdi H. Saad
Keyword(s):  
Heavy Metals ◽  
Inductively Coupled Plasma ◽  
Blue Nile ◽  
Heavy Metal Concentrations ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Zn Concentration ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Two Sides

The research cared out to calculate the heavy metals deposition for the soil of the Blue Nile along the edges of two sides, starting from the Sudan Ethiopia border end to the Khartoum state in Tuti Island. The samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) technique. The results show that there is a significant difference for most of the concentration of the elements deposition on two sides of the Blue Nile outside of Nile channel for that area which covered by water after the overflow. Heavy metals Cr, Fe, Co, Cu, and Zn concentration deposition are increased in the direction of the water to Khartoum, while Ni and Pb concentrations are decreased in the same direction. K, Ca, Ti, Mn, Br, Rb, Sr, and Zr heavy metal concentrations are varied but in general remains equals.

Selenium compounds in selenium-enriched cabbage

2012 ◽  
Vol 84 (2) ◽  
pp. 259-268 ◽  
Author(s):  
Špela Mechora ◽  
Mateja Germ ◽  
Vekoslava Stibilj
Keyword(s):  
Brassica Oleracea ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
High Dose ◽  
Selenium Compounds ◽  
Red Cabbage ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Stems And Leaves

For the study, cabbage (Brassica oleracea var. capitata L.) and red cabbage (Brassica oleracea var. capitata L. f. rubra) were treated with Na selenate. Cabbage was foliarly sprayed twice with 20 mg Se(VI) L–1, while red cabbage was fertilized twice with 0.5 mg Se(VI) L–1. Despite the high dose of Se, no toxic effects were observed on cabbage plants. The total Se concentration in cabbage leaves was 4.80 ± 0.25 μg Se g–1 (DM) and in red cabbage 0.96 ± 0.04 μg Se g–1 (DM). Soluble Se compounds were extracted from parts of cabbage with protease XIV, resulting in 49 % of soluble Se from roots, 59 % from leaves, and 65 % from stems. In red cabbage, the corresponding figures were 28 % of soluble Se in roots, 31 % in stems, and 43 % in leaves. Se species were determined in the enzymatic extracts using ion exchange high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The main Se species found in all parts of cabbage and red cabbage was selenomethionine (SeMet), which in roots represented 94 and 55 % of the soluble Se content in cabbage and red cabbage, respectively. In stems and leaves of cabbage, SeMet represented only 23 % of the soluble Se content. In stems of red cabbage SeMet represented 80 % and in leaves 41 % of the soluble Se content. We observed that traces of Se(VI) were present in upper parts of both plants.

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