scholarly journals Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
He-Qing Wang ◽  
Hang Yang ◽  
Qian Tang ◽  
Yi-Chen Gong ◽  
Yuan-Hao Fu ◽  
...  
Keyword(s):  
Aortic Dissection ◽  
Acute Aortic Dissection ◽  
Early Stage ◽  
Serum Biomarkers ◽  
Label Free ◽  
Serum Samples ◽  
Proteomics Analysis ◽  
Proteomics Approach ◽  
Healthy Control ◽  
High Level

Acute aortic dissection (AAD) is an emergent vascular disease. Currently, its diagnosis depends on clinical and radiological investigations but lacking of serum biomarkers. In this study, we aimed to identify potential serum biomarkers for AAD using label-free proteomics approach. A total of 90 serum samples were collected from three groups: patients with acute aortic dissection (AAD, n=30), patients with acute myocardial infarction (AMI, n=30), and healthy controls (n=30), and the first four samples from each group were selected for label-free proteomics analysis. Using label-free approach, a total of 22 differentially expressed proteins were identified in the serum samples of the AAD group, of which 15 were upregulated and 7 were downregulated as compared to the AMI and healthy control groups. The most prominent increased protein was vinculin, which was selected to validate in total samples. The level of vinculin was significantly elevated in AAD patients (15.8 ng/ml, IQR: 9.3-19.9 ng/ml) than that in AMI patients (8.6 ng/ml, IQR:5.3-11.4 ng/ml) and healthy volunteers (5.3 ng/ml, IQR:2.8-7.6 ng/ml), P<0.0001. Furthermore, the concentration of vinculin both increased in type A and B dissection. At the early stage of AAD, vinculin maintained a high level to 48 hours compared with that of AMI. Our study demonstrated that vinculin may play a role in the early diagnosis of AAD.

scholarly journals Quantitative Proteomics Analysis by Isobaric Tags for Relative and Absolute Quantitation Identified Lumican as a Potential Marker for Acute Aortic Dissection

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Guorong Gu ◽  
Weizhong Cheng ◽  
Chenling Yao ◽  
Jun Yin ◽  
Chaoyang Tong ◽  
...  
Keyword(s):  
Aortic Dissection ◽  
Quantitative Proteomics ◽  
Acute Aortic Dissection ◽  
Serum Marker ◽  
Serum Biomarkers ◽  
Serum Samples ◽  
Absolute Quantitation ◽  
Itraq Analysis ◽  
Potential Marker ◽  
Isobaric Tags

Acute aortic dissection (AAD) is a serious vascular disease. Currently the diagnosis relies on clinical and radiological means whereas serum biomarkers are lacking. The purpose of this study was to identify potential serum biomarkers for AAD using isobaric tags for relative and absolute quantitation (iTRAQ) approach. A total of 120 serum samples were collected from three groups: AAD patients (n=60), patients with acute myocardial infarction (AMI,n=30), and healthy volunteers (n=30), whereas the first 10 samples from each group were used for iTRAQ analysis. Using iTRAQ approach, a total of 174 proteins were identified as significantly different between AAD patients and healthy subjects. Among them, forty-six proteins increased more than twofold, full-scale analysis using serum sample for the entire 120 subjects demonstrated that Lumican level was significantly increased relative to control and AMI samples. Further, Lumican level correlated with time from onset to admission in AAD but not AMI samples. Using iTRAQ approach, our study showed that Lumican may be a potential AAD-related serum marker that may assist the diagnosis of AAD.

scholarly journals Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection

2020 ◽  
Vol 19 (11) ◽  
pp. 1749-1759 ◽  
Author(s):  
Xin Hou ◽  
Xiaomei Zhang ◽  
Xian Wu ◽  
Minya Lu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Serum Proteins ◽  
Early Stage ◽  
Protein Profiling ◽  
Cytokine Signaling ◽  
Large Set ◽  
Serum Samples ◽  
Antibody Microarray ◽  
Proteomics Analysis ◽  
Immune Signaling ◽  
Anti Inflammation

Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n = 15) and influenza (n = 13) patients. We identified a large set of differentially expressed proteins (n = 132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

Serum Calprotectin as a Blood-Based Biomarker for Monitoring Knee Osteoarthritis at Early but Not Late Stages

Cartilage ◽  
2020 ◽  
pp. 194760352096116
Author(s):  
Amin Safa ◽  
Abolfazl Bagherifard ◽  
Hamadalla Hadi Al-Baseesee ◽  
Azade Amini Kadijani ◽  
Hooman Yahyazadeh ◽  
...  
Keyword(s):  
Disease Duration ◽  
Early Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Knee Oa ◽  
Calprotectin Level ◽  
Healthy Control ◽  
Grade Ii ◽  
Late Stages

Objective The identification of early-stage osteoarthritis (OA) is crucial for the deceleration of its progression; however, no reliable biomarker is available for this purpose. The current study aimed to determine the role of serum calprotectin in the detection of early-stage knee OA. Design In a case-control study, serum samples were collected from 84 patients with primary bilateral knee OA and 52 healthy controls. The radiographic grading of knee OA was performed using the Kellgren-Lawrence classification system. Serum concentrations of calprotectin were measured using an enzyme-linked immunosorbent assay. Results The mean serum calprotectin level was 2908 ± 2516 ng/mL in OA patients and 901 ± 875 ng/mL in healthy control subjects ( P < 0.001). Mean serum calprotectin levels were significantly higher in the lower stages of OA: 3740 ± 2728 ng/mL in OA grade I, 3100 ± 2084 ng/mL in OA grade II, 2246 ± 1418 ng/mL in OA grade III, and 2035 ± 765 ng/mL in OA grade IV ( P = 0.047). Serum calprotectin levels were significantly higher in patients with a disease duration <42 months compared with those with a disease duration >42 months ( P = 0.043). Conclusion Serum calprotectin level increases significantly in the early stages of OA and shows a reverse association with disease severity. Therefore, it could be suggested as a promising blood-based marker for early-stage knee OA.

scholarly journals Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 84 ◽  
Author(s):  
Dan Tao ◽  
Bingqing Shui ◽  
Yingying Gu ◽  
Jing Cheng ◽  
Weiying Zhang ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Early Stage ◽  
Differential Pulse ◽  
Electrical Signal ◽  
Label Free ◽  
Modified Glassy Carbon Electrode ◽  
Serum Samples ◽  
Pulse Voltammetry ◽  
Aptamer Sensor ◽  
Preliminary Application

The electrochemical aptamer sensor has been designed for detecting tau381, a critical biomarker of Alzheimer′s disease in human serum. The aptasensor is obtained by immobilizing the aptamer on a carboxyl graphene/thionin/gold nanoparticle modified glassy-carbon electrode. As a probe and bridge molecule, thionin connected carboxyl graphene and gold nanoparticles, and gave the electrical signal. Under optimal conditions, the increment of differential pulse voltammetry signal increased linearly with the logarithm of tau381 concentration in the range from 1.0 pM to 100 pM, and limit of detection was 0.70 pM. The aptasensor reliability was evaluated by determining its selectivity, reproducibility, stability, detection limit, and recovery. Performance analysis of the tau381 aptasensor in 10 patients’ serum samples showed that the aptasensor could screen patients with and without Alzheimer′s disease. The proposed aptasensor has potential for use in clinically diagnosing Alzheimer′s disease in the early stage.

scholarly journals Acute Aortic Dissection Biomarkers Identified Using Isobaric Tags for Relative and Absolute Quantitation

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ziya Xiao ◽  
Yuan Xue ◽  
Chenling Yao ◽  
Guorong Gu ◽  
Yaping Zhang ◽  
...  
Keyword(s):  
Aortic Dissection ◽  
Sensitivity And Specificity ◽  
Acute Aortic Dissection ◽  
Roc Curves ◽  
Diagnostic Value ◽  
Serum Samples ◽  
Absolute Quantitation ◽  
Isobaric Tags ◽  
Diagnostic Sensitivity And Specificity ◽  
D Dimer

The purpose of this study was to evaluate the utility of potential serum biomarkers for acute aortic dissection (AAD) that were identified by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approaches. Serum samples from 20 AAD patients and 20 healthy volunteers were analyzed using iTRAQ technology. Protein validation was performed using samples from 120 patients with chest pain. A total of 355 proteins were identified with the iTRAQ approach; 164 proteins reached the strict quantitative standard, and 125 proteins were increased or decreased more than 1.2-fold (64 and 61 proteins were up- and downregulated, resp.). Lumican, C-reactive protein (CRP), thrombospondin-1 (TSP-1), and D-dimer were selected as candidate biomarkers for the validation tests. Receiver operating characteristic (ROC) curves show that Lumican and D-dimer have diagnostic value (area under the curves [AUCs] 0.895 and 0.891,P<0.05). For Lumican, the diagnostic sensitivity and specificity were 73.33% and 98.33%, while the corresponding values for D-dimer were 93.33% and 68.33%. For Lumican and D-dimer AAD combined diagnosis, the sensitivity and specificity were 88.33% and 95%, respectively. In conclusion, Lumican has good specificity and D-dimer has good sensitivity for the diagnosis of AAD, while the combined detection of D-dimer and Lumican has better diagnostic value.

A simple model predicting in-hospital death in patients with type A acute aortic dissection

Perfusion ◽  
2021 ◽  
pp. 026765912110297
Author(s):  
Min Wang ◽  
Liyan Luo ◽  
Xiaohong Xia ◽  
Jiahong Jiang ◽  
Litao Zhang ◽  
...  
Keyword(s):  
High Risk ◽  
Aortic Dissection ◽  
Acute Aortic Dissection ◽  
Early Stage ◽  
Characteristic Curve ◽  
Type A ◽  
Hospital Death ◽  
High Morbidity ◽  
Distribution Width ◽  
D Dimer

Background: Type A acute aortic dissection (TAAAD) is a destructive cardiovascular disease, with high morbidity and mortality rates. Identifying the high-risk TAAAD patients at an early stage is urgently necessary. Methods: A retrospective study of 160 patients was carried out. The admission data were retrospectively gathered. Logistic regression analysis and receiver operator characteristic curve (AUC) was utilized. Results: Compared with the survivor group, the nonsurvivor group was older, had higher D-dimer levels, red blood cell distribution width (RDW) levels and platelet distribution width (PDW) levels, and lower fibrinogen levels, platelet levels and plateletcrit levels. Multivariate analysis displayed that four independent factors, age (hazard ratio (HR): 7.877, 95% confidence interval (CI) 2.740–22.641, p < 0.001), D-dimer (HR: 3.791, 95% CI 1.520–9.452, p = 0.004), RDW (HR: 3.300, 95% CI 1.109–9.825, p = 0.032), PDW (HR: 3.755, 95% CI 1.436–9.815, p = 0.007) were incorporated into the model. The predict accuracy of the model (AUC 0.861, 95% CI 0.798–0.911, p < 0.001) was best. Conclusions: Age, D-dimer, RDW and PDW are independent markers of in-hospital death in TAAAD patients and the newly established model has better performance in predicting high-risk patients. This model can be used as a quick screening tool to assess the prognosis of patients in individualizing.

Evaluation of Some Immunological Marker for Early Diagnosis of Rheumatoid Arthritis

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Israa K AL- Yasiri ◽  
Israa K AL- Yasiri ◽  
Jaafar K Al-Mousawi ◽  
Ali M.Al Mohana
Keyword(s):  
Rheumatoid Arthritis ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Interleukin 1 ◽  
Joint Damage ◽  
Serological Marker ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Significant Difference ◽  
Healthy Control

Rheumatoid arthritis (RA) is a chronic,destructive autoimmune disease affecting the joints. With more sophisticated and effective therapies becoming available and with the understanding that early intervention is crucial in preventing irreversible joint damage,it is more and more important to diagnose RA at a very early stage in the disease. To facilitate diagnosis during the early stages of the disease,when often not all clinical symptoms are manifest,a good serological marker is needed. The main purpose of this observational study was to evaluate and detects a good serological marker and genetic factors may be used in early detection of RA. This study covers quite different regions of Najaf province,including rural and urban communities. During the period from May 2010 to May2011,a total of 40 patients with RA who were fulfilled four or more of the 1987 American College of Rheumatology (ACR),20 patients with joints problems (JP),20 RA patient relatives (PR) and 10 apparently healthy control individuals were included in this study. Cytokines (interleukin-1,IL-6,IL-10 and TNF ɑ),antibodies directed to cyclic citrullinated peptide (anti-CCP3),and anti-human immunoglobulin binding protein (anti-BiP) antibodies in the human serum samples were measured by enzyme-linked immunosorbent assay (ELISA). Thirty-two RA patients (80.0%) were positive for anti-CCP3,whereas only 20.0%,30.0% and 0.0% showed a positive reaction,in particular sera from PR,JP and healthy control,respectively with highly significant difference was observed. The mean serum anti-CCP3 antibody level was higher in RA patients than in other groups. The anti-BiP antibody levels were significantly increased in RA patients than in PR and healthy control. However,the levels of anti-BiP antibody were slightly increased in the JP and no significant difference was detected between RA patients and JP. Analysis of the serum samples showed that concentrations of the Proinflammatory cytokines,IL-1,IL-6 and TNFɑ were significantly increased in RA patients compared with matched control subjects. While,The level of IL-10 was significantly lower for RA patients than for healthy Control cases.

scholarly journals Serum protein profiling reveals a landscape of inflammation and immune signaling in early-stage COVID-19 infection

2020 ◽  
Author(s):  
Xin Hou ◽  
Xiaomei Zhang ◽  
Xian Wu ◽  
Minya Lu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Serum Proteins ◽  
Early Stage ◽  
Protein Profiling ◽  
Cytokine Signaling ◽  
Large Set ◽  
Serum Samples ◽  
Antibody Microarray ◽  
Proteomics Analysis ◽  
Immune Signaling ◽  
Anti Inflammation

Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n=15) and influenza (n=13) patients. We identified a large set of differentially expressed proteins (n=125) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

scholarly journals Dynamic Characteristic Analysis of Antibodies in Patients With COVID-19: A 13-Month Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Danrong Shi ◽  
Tianhao Weng ◽  
Jie Wu ◽  
Chunyan Dai ◽  
Rui Luo ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Early Stage ◽  
Receptor Binding Domain ◽  
Characteristic Analysis ◽  
Serum Samples ◽  
Long Time ◽  
High Level ◽  
Ig G ◽  
Post Onset

There is a worldwide pandemic of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection; yet our understanding remains limited on the characteristic of antibodies, especially for dynamic long-term tracking. Sequential serum samples were collected up to 416 days post onset of symptoms (POS) from 102 patients who were hospitalized with coronavirus disease 2019 (COVID-19). Immunoglobulin (Ig)G, IgM, and IgA levels targeting SARS-CoV-2 spike 1 receptor-binding domain (S1-RBD), spike 2 extracellular domain (S2-ECD), and nucleocapsid protein (N) were quantified as well as neutralizing activity. We were pleasantly surprised to find that the antibody remained detective and effective for more than a year POS. We also found the varied reactions of different antibodies as time passed: N-IgA rose most rapidly in the early stage of infection, while S2-IgG was present at a high level in the long time of observation. This study described the long traceable antibody response of the COVID-19 and offered hints about targets to screen for postinfectious immunity and for vaccination development of SARS-CoV-2.

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