scholarly journals Increased Intraplatelet ADMA Level May Promote Platelet Activation in Diabetes Mellitus

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jakub Gawryś ◽  
Jerzy Wiśniewski ◽  
Ewa Szahidewicz-Krupska ◽  
Damian Gajecki ◽  
Julia Leśniewska ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Risk Factors ◽  
Platelet Aggregation ◽  
Platelet Activation ◽  
Laser Doppler Flowmetry ◽  
Asymmetric Dimethylarginine ◽  
Organ Damage ◽  
Doppler Flowmetry

Background. Antiplatelet therapy has become a standard therapeutic approach in the secondary prevention of cardiovascular system disorders of thrombotic origin. Patients with concomitant diabetes mellitus (DM) obtain fewer benefits from this treatment. Hence, the pathophysiology of altered platelet function in response to glucose metabolism impairment should be of particular interest. Objectives. The aim of our study was to verify if the platelet expression of the asymmetric dimethylarginine (ADMA) in diabetic patients differs in comparison to the nondiabetic ones. The correlation of platelet-ADMA with platelet activation and aggregation as well as with other risk factors was also investigated. Material and Methods. A total of 61 subjects were enrolled in this study, including thirty-one type 2 diabetic subjects without diabetes-related organ damage. Physical examination was followed by blood collection with an assessment of platelet aggregation, traditional biochemical cardiovascular risk factors, and evaluation of nitric oxide bioavailability parameters in plasma and thrombocytes. Subsequently, the assessment of endothelial function using Peripheral Arterial Tonometry and Laser Doppler Flowmetry (LDF) was performed. Results. In the DM group, elevated concentration of intraplatelet ADMA and higher ADMA/SDMA ratio compared to the control group was observed. It was accompanied by higher ADP-mediated platelet aggregation and lower microvascular response to a local thermal stimulus measured by LDF in the diabetes group. Conclusions. Type 2 diabetes is related to higher intraplatelet concentration of asymmetric dimethylarginine (ADMA), which may result in impaired platelet-derived nitric oxide synthesis and subsequent increased platelet activity, as assessed by the ADP-induced aggregation. Laser Doppler Flowmetry, compared to EndoPAT 2000, appears to be a more sensitive indicator of the impaired microvasculature vasodilation in diabetics without the presence of clinically significant target organ damage.

METHOD FOR ANALYSIS OF THE DOPPLER-BROADENED SPECTRA OF LASER RADIATION FOR THE ASSESSMENT OF BLOOD MICROCIRCULATION DISTURBANCES IN DIABETES MELLITUS TYPE 2

2020 ◽  
Vol 4 (1) ◽  
pp. 80-87
Author(s):  
I.O. KOZLOV
Keyword(s):  
Diabetes Mellitus ◽  
Laser Radiation ◽  
Laser Doppler Flowmetry ◽  
Diabetes Mellitus Type 2 ◽  
Feature Space ◽  
Diabetes Mellitus Type ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
New Feature

The article is devoted to the development of laser Doppler flowmetry and analysis of the recorded signal to study the distribution of perfusion over the frequencies of Doppler broadening of laser radiation. The processing algorithm and the necessary technical conditions for the correct registration of the signal are shown. As examples of the proposed method implementation, the data are obtained from a healthy volunteer and a patient with diabetes mellitus type 2 and analyzed. According to the proposed method, processing of recorded data provides a new feature space for data analysis of laser Doppler flowmetry signal.

Evaluation of Biochemical and Clinical Parameters of Hypertension with Type 2 Diabetes Mellitus

2018 ◽  
Vol 69 (9) ◽  
pp. 2402-2406 ◽  
Author(s):  
Patricia Nicola ◽  
Elena Ardeleanu ◽  
Carmen Gadau ◽  
Maria Dorobantu ◽  
Roxana Darabont ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Blood Pressure ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Ldl Cholesterol ◽  
Target Organ Damage ◽  
Organ Damage ◽  
Office Blood Pressure

The aim of this study was to assess the basic biochemical and clinical characteristics of patients with hypertension and type 2 diabetes mellitus (T2DM), office blood pressure (BP) and 24-h BP profile, their risk factors and associated comorbidities. Compared with non-diabetics, hypertensive patients with T2DM were older, with a longer duration of hypertension (5.9 vs. 4.7 years), greater office blood pressure and ambulatory BP values, increased incidence of multiple risk factors, target organ damage and cardiovascular disease. Biochemical data in hypertension with T2DM revealed significantly high levels of LDL cholesterol, triglycerides, creatinine, micro- and macro-albuminuria and a reduced estimated glomerular filtration rate. The presence of diabetes was associated with obesity, represented by a BMI ]30 kg/m2 (OR 2.08 [95% CI 1.26-3.45], p = 0.004), abdominal obesity (OR 1.85 [95% CI 1.11-3.04], p = 0.016), high LDL cholesterol (OR 2.02 [95% CI 1.22-3.35], p = 0.006) and high triglycerides (OR 1.86 [95% CI 1.11-3.11], p = 0.017).

Concomitant peripheral neuropathy and type 2 diabetes impairs post-exercise cutaneous perfusion and flowmotion

2021 ◽  
Author(s):  
Cécile Reynès ◽  
Jean-Baptiste Beaume ◽  
Françoise Latil-Plat ◽  
Houda Ennaifer ◽  
Laure Rocher ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Type 2 Diabetes ◽  
Peripheral Neuropathy ◽  
Laser Doppler Flowmetry ◽  
Blood Perfusion ◽  
Laser Speckle ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
Post Exercise

Abstract Aim The study investigated changes in microvascular perfusion during post-exercise recovery in those with type 2 diabetes, with or without peripheral neuropathy, as well as in healthy controls and those with obesity. Methods Skin blood perfusion was assessed in each group using laser doppler flowmetry and laser speckle contrast imaging before and immediately after a six-minute walking test. Laser doppler flowmetry recordings underwent wavelet transformation to allow specific control mechanisms of blood perfusion to be studied (e.g. endothelial nitric oxide independent and dependent, neurogenic, myogenic, respiratory and cardiac mechanisms). Results Skin blood perfusion increased after exercise in all groups (22.3±28.1% with Laser speckle contrast imaging and 22.1±52.5% with laser doppler flowmetry). Throughout post-exercise recovery, the decrease was blunted in those with subclinical peripheral neuropathy and confirmed peripheral neuropathy when compared to the other three groups. After exercise, total spectral power increased in all groups. The relative contributions of each endothelial band was lower in those with confirmed peripheral neuropathy than in the healthy controls and those with obesity (nitric oxide-dependent function: 23.6±8.9% versus 35.5±5.8% and 29.3±8.8%, respectively; nitric oxide-independent function: 49.1±23.7% versus 53.3±10.4% and 64.6±11.4%, respectively). The neurogenic contribution decreased less in those with confirmed peripheral neuropathy and in those with type 2 diabetes alone, compared to those with subclinical peripheral neuropathy and those with obesity (-14.5±9.9% and -12.2±6.1% versus -26.5±4.7% and -21.7±9.4%, respectively). Conclusion Peripheral neuropathy, whatever the stage, altered the microvascular response to exercise via impaired endothelial and neurogenic mechanisms.

scholarly journals Features of Cardiovascular Risk Factors, Target Organ Damage in Patients with Diabetes Mellitus Type 2 and Arterial Hypertension

Kardiologiia ◽  
2019 ◽  
Vol 59 (9) ◽  
pp. 20-28
Author(s):  
S. Kh. Mehdiyev ◽  
I. I. Mustafaev ◽  
M. N. Mamedov
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Type 2 Diabetes ◽  
Arterial Hypertension ◽  
Target Organ Damage ◽  
Organ Damage ◽  
Left Ventricular ◽  
Target Organ ◽  
Target Organs

Aim: to investigate relationship between arterial hypertension (AH) and risk factors / subclinical damage of target organs in patients with type 2 diabetes mellitus (DM2). Methods. We included into this clinical epidemiological study 528 patients with DM2 (30.5 % men, 69.5 % women; mean age 54.1±0.3 years; 80.3 % with AH, 19.7 % without AH), who answered questions of the ARIC study questionnaire related to risk factors. Also, we studied features of target organ damage and laboratory indicators. Results. In comparison with normotensives patients with AH more frequently had ischemic heart disease (12.7±1.6 % vs. 5.8±2.3 %, p<0.05), chronic heart failure (CHF) (30.9±2.2 % vs. 9.6±2.9 %, p<0.001), atherosclerosis of vessels of lower extremities (69.8±2.2 % vs. 53.8±4.9 %, p<0.01) and cerebral vessels (50.9±2.4 % vs. 28.8±4.4 %, p<0.001), history of stroke (5.0±1.1 % vs. 0 %, p<0.05), hypertonic angiopathy (14.5±1.8 % vs. 6.5±2.5 %, p<0.05), low level of high density lipoprotein (87.3±2.2 % vs. 74.5±6.4 %, p<0.05), electro- and echocardiographic signs of left ventricular hypertrophy (75.6±2.1 % vs. 45.4±5.1 %, p<0.001; 61.1±2.6 % vs. 24.4±4.7 %, p<0.001, respectively), lowering of left ventricular ejection fraction (12.5±1.7 % vs. 7.8±2.8 %, p<0.001), diastolic disfunction of the left ventricle (52.6±2.7 % vs. 23.2±4.7 %, p<0.001), atherosclerosis of the aorta (38.0±2.6 % vs. 20.7±4.5 %, p<0.01), lowering of the ankle-brachial index (left – 29.8±2.3 % vs. 14.9±3.5 %, p<0.01; right – 31.5±2.3 % vs. 9.9±3.0 %, p<0.001, respectively), increased intima-media thickness of the right carotid artery (84.6±5.0 % vs. 60.0±11.0 %, p<0.05). Conclusion. In patients with type 2 diabetes and AH, in order to develop strategy of macro- and microvascular complications prevention, it is necessary to conduct early screening of risk factors and subclinical damage of target organs.

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