scholarly journals The Correlation between Hormonal Disturbance in PCOS Women and Serum Level of Kisspeptin

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Razaw O. Ibrahim ◽  
Shirwan H. Omer ◽  
Chro N. Fattah
Keyword(s):  
Menstrual Cycle ◽  
Polycystic Ovary ◽  
Essential Element ◽  
Free Testosterone ◽  
Serum Levels ◽  
Normal Weight ◽  
Normal Women ◽  
And Control ◽  
Lh Secretion ◽  
Ovarian Syndrome

Background. Kisspeptin is a neuropeptide that upregulates gonadotropin-releasing hormone (GnRH) secretion. It is an essential element for the luteinizing hormone (LH) surge and ovulation. Women with polycystic ovary syndrome (PCOS) expose alteration in both GnRH and LH secretion levels. Objective. This paper aims to evaluate serum kisspeptin levels in healthy and polycystic ovarian syndrome women. Furthermore, it investigates the effect of obesity and age on circulating kisspeptin levels in both normal and PCOS women. Moreover, it points out the correlation between kisspeptin and other hormonal parameters. Methods and Patients. One hundred women (60 are with PCOS and 40 are normal) were enrolled in the study. Five milliliter samples of blood from all the patients and control women were obtained twice during the menstrual cycle. All the study samples were classified depending on the age factor for several subgroups. Results. Kisspeptin levels were higher in PCOS patients than those in the normal group. Kisspeptin correlated with serum free testosterone level (r=0.26). In healthy women, preovulatory kisspeptin levels were higher than follicular kisspeptin levels (P<0.05), while this difference was insignificant in PCOS patients. The variation in serum kisspeptin levels between overweight/obese and normal-weight women was insignificant. In normal women, serum kisspeptin levels were higher in women >35 years than those <24 years at (P=0.03). Conclusion. The serum kisspeptin level is higher in PCOS women. Its levels fluctuate during the menstrual cycle, but these fluctuations are disturbed in PCOS women. The effect of BMI on serum kisspeptin levels is insignificant, and kisspeptin serum levels increase with age.

scholarly journals Hyperandrogenism by Liquid Chromatography Tandem Mass Spectrometry in PCOS: Focus on Testosterone and Androstenedione

2020 ◽  
Vol 10 (1) ◽  
pp. 119
Author(s):  
Giorgia Grassi ◽  
Elisa Polledri ◽  
Silvia Fustinoni ◽  
Iacopo Chiodini ◽  
Ferruccio Ceriotti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Polycystic Ovary ◽  
Free Testosterone ◽  
Reference Intervals ◽  
Model Assessment ◽  
Homeostatic Model Assessment ◽  
Liquid Chromatography Tandem Mass ◽  
And Control ◽  
The Impact

The identification of hyperandrogenism in polycystic ovary syndrome (PCOS) is concerning because of the poor accuracy of the androgen immunoassays (IA) and controversies regarding which androgens should be measured. The aim of our study was to evaluate the impact of the assessment of testosterone (T) and androstenedione (A) by liquid chromatography in tandem with mass spectrometry (LC/MS-MS), in the diagnosis of PCOS. We evaluated 131 patients referred for suspected PCOS. Fourteen patients in total were excluded, some because of other diagnosis (n = 7) or incomplete diagnostic workup (n = 7). We measured T and A both by IA and LC-MS/MS in the 117 subjects included. We calculated free T (fT) by the Vermeulen formula and recorded clinical and metabolic data. 73 healthy females served as controls to derive immunoassays (IA) and LC-MS/MS reference intervals for T, fT and A. PCOS was confirmed in 90 subjects by IA and in 93 (+3.3%) by LC-MS/MS. The prevalence of biochemical hyperandrogenism in PCOS by LC-MS/MS increased from 81.7% to 89.2% if A was also considered. The most frequently elevated androgens were fT (73.1%) and A (64.5%) and they had similar levels of accuracy in differentiating PCOS and controls (0.34 ng/dL, Sn 91% Sp 89%; 1.16 ng/mL, Sn 91% Sp 88%, respectively). Free testosterone correlated with body mass index (BMI), homeostatic model assessment (HOMA)-index, glycated hemoglobin (HbA1c), and sex-binding globulin (SHBG). The results confirm that LC-MS/MS is slightly more sensitive than IA in the diagnosis of PCOS with LC-MS/MS detecting higher levels of fT and A. Moreover, assessment of fT and A by LC-MS/MS had a similar level of accuracy in discriminating between PCOs and control subjects. Lastly, fT by LC-MS/MS correlates with adverse metabolic parameters.

Alterations of the hypothalamic GnRH interpulse interval sequence over the normal menstrual cycle

1988 ◽  
Vol 255 (5) ◽  
pp. E696-E701 ◽  
Author(s):  
N. Santoro ◽  
J. P. Butler ◽  
M. Filicori ◽  
W. F. Crowley
Keyword(s):  
Menstrual Cycle ◽  
Renewal Process ◽  
Gnrh Secretion ◽  
Late Luteal Phase ◽  
Normal Menstrual Cycle ◽  
Lh Release ◽  
Interval Sequence ◽  
Normal Women ◽  
Normal Men ◽  
Lh Secretion

Luteinizing hormone (LH) is released in a pulsatile fashion from the anterior pituitary in response to hypothalamic gonadotropin-releasing hormone (GnRH) secretion. Previous autocorrelation analysis of the sequence of interpulse intervals of LH secretion in normal men has supported the hypothesis that the underlying hypothalamic mechanism of GnRH secretion governing episodic LH release is a renewal process, indicating that hypothalamic "memory," if present, does not extend back further in time than the preceding secretory pulse. A similar analysis of pulsatile LH secretion was undertaken in 45 studies of normal women, obtained throughout the menstrual cycle. Analysis of these studies revealed a process consistent with renewal throughout the follicular and early luteal phases. However, this relationship appears to break down in the mid-to-late luteal phase, indicating that alternative feedback pathways provide an overriding influence on the underlying renewal process of hypothalamic GnRH secretion. Pulsatile progesterone secretion by the corpus luteum, which first emerges at this stage of the menstrual cycle, may be the agent responsible for this feedback.

THE INFLUENCE OF COMBINED CYPROTERONE ACETATE-ETHINYL OESTRADIOL THERAPY ON SERUM LEVELS OF DEHYDROEPIANDROSTERONE, ANDROSTENEDIONE, AND TESTOSTERONE IN HIRSUTE WOMEN

1977 ◽  
Vol 84 (2) ◽  
pp. 333-342 ◽  
Author(s):  
Marijke Frölich ◽  
Nadia Lachinsky ◽  
A. J. Moolenaar
Keyword(s):  
Linear Correlation ◽  
Cyproterone Acetate ◽  
Polycystic Ovary ◽  
Serum Levels ◽  
Ovary Syndrome ◽  
Testosterone Levels ◽  
Normal Women ◽  
Peripheral Conversion ◽  
Ethinyl Oestradiol

ABSTRACT Serum levels of dehydroepiandrosterone, androstenedione, and testosterone in hirsute women suffering from either idiopathic hirsutism or the polycystic ovary syndrome were determined before and during treatment with cyproterone acetate combined with ethinyl oestradiol. During this treatment the hirsutism decreased markedly. In untreated hirsute women serum dehydroepiandrosterone levels do not differ from those in normal women and do not change during therapy; androstenedione levels are higher than normal and decrease markedly during treatment, and the testosterone levels are elevated compared to normals, with a considerable overlap, and show only a tendency to decrease. There is no correlation between dehydroepiandrosterone and androstenedione levels or between androstenedione and testosterone levels in the serum of untreated hirsute women. After 12–14 months of cyproterone acetate-ethinyl oestradiol therapy a linear correlation is found between dehydroepiandrosterone and androstenedione levels. This can be explained by the relatively higher contribution of the peripheral conversion of dehydroepiandrosterone to androstenedione to the occurrence of lower androstenedione levels. The linear correlation between androstenedione and testosterone after treatment, also found in normal women, indicates the restoration of the role of androstenedione as the major peripheral precursor of testosterone.

scholarly journals Serum Leptin Concentrations in Women with Polycystic Ovary Syndrome

1997 ◽  
Vol 82 (6) ◽  
pp. 1697-1700 ◽  
Author(s):  
Juha Rouru ◽  
Leena Anttila ◽  
Pertti Koskinen ◽  
Tuula-Anneli Penttilä ◽  
Kerttu Irjala ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Serum Insulin ◽  
Univariate Analysis ◽  
Serum Triglyceride ◽  
Free Testosterone ◽  
Ovary Syndrome ◽  
Serum Leptin ◽  
Control Subjects ◽  
And Control

Abstract The role of gonadotropins, androgens, and insulin in the regulation of circulating leptin levels is obscure. In order to clarify the relationships of these parameters we studied serum leptin levels in 19 healthy control subjects and in 35 hyperandrogenic and hyperinsulinemic patients with polycystic ovary syndrome (PCOS). Serum leptin concentrations did not differ significantly between PCOS patients and control subjects. When PCOS and control groups were analyzed together by univariate analysis, serum leptin was positively correlated with body mass index (BMI), body weight, serum insulin, serum triglyceride, and serum free testosterone concentrations. Serum leptin was inversely correlated with serum sex hormone binding globulin (SHBG) concentrations. There were no significant correlations between serum leptin and testosterone, androstenedione, or gonadotropin concentrations. Serum insulin, triglyceride, and free testosterone concentrations were positively correlated, and serum SHBG was negatively correlated with BMI. However, when BMI on one hand and serum insulin, triglyceride, free testosterone, or SHBG on other hand were used as independent variables in the partial correlation analysis with leptin, BMI turned out to be the variable primarily responsible for all of the correlations with leptin. In conclusion, the concept that circulating leptin levels would be different in PCOS patients than in regularly menstruating control subjects is not supported by our data.

scholarly journals POLYCYSCTIC OVARIAN SYNDROME (PCOS);

2018 ◽  
Vol 21 (02) ◽  
pp. 354-359
Author(s):  
Rashida Perveen ◽  
Sobia Hamid ◽  
Mehnaz Khakwani
Keyword(s):  
Menstrual Cycle ◽  
Polycystic Ovary ◽  
Semen Analysis ◽  
Clomiphene Citrate ◽  
Study Group ◽  
Polycystic Ovaries ◽  
Clinical Trail ◽  
Group A ◽  
Ovarian Syndrome ◽  
Urine Pregnancy Test

Objective: To compare effectiveness of clomiphene alone and in combinationwith metformin in terms of conception rate in patients presenting with polycystic ovary syndrome.Study Design: Randomized clinical trail. Place and duration of study: Department Obstetricsand Gynecology Unit I, Nishtar Hospital Multan, 6 months from 12th September 2009 to 11thMarch 2010. Methodology: Primary subfertile women visiting outpatient department of NishtarHospital Multan with significant irregular menstrual cycle, oligo menorrhea and ultrasonographicfeatures of polycystic ovaries were enrolled in the study. Semen samples from male partners wereanalyzed according to the WHO criteria. Women whose partner’s semen analysis was abnormalwere excluded from study. Group ‘A’ was given clomiphene citrate alone and second group (i.e.group B) was given colmiphene citrate and metformin. All patients were followed till six cycles forregularity of menstrual cycle (determined by history) ovulation by follicular tracking onultrasonography, if patient (conceived), conception was confirmed by urine pregnancy test andgestational sac on ultrasonography pelvis. Final outcome was measured at six month.Information was entered in specifically designed proforma. Results: Frequency of PCOS was14.05%. Mean age of the patients was 31.03 ± 0.58 vs. 29.89 ± 0.53 years in group A and Brespectively. Oligomenorrhea was present in 18(50%) vs. 18 (50%) women, amenorrhea in7(19.4%) vs. 8(22.2%) patients and weight gain in 14(38.9%) vs. 15(41.7%) cases in group A andB respectively. Conception was achieved in 26(72.2%) vs. (18(50.0%) patients in group A and Brespectively. Conclusions: Metformin does not seem to help in achieving higher rates ofpregnancy when combined with CC as compared to CC alone.

scholarly journals Metastatin as a Marker for Hyperandrogenemia in Iraqi Women with Polycystic Ovary Syndrome

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Manal Madany Abdalqader ◽  
Shatha Sami Hussein
Keyword(s):  
Roc Analysis ◽  
Polycystic Ovarian Syndrome ◽  
Case Control Study ◽  
Polycystic Ovary ◽  
Free Testosterone ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Iraqi Women ◽  
Ovarian Syndrome ◽  
Control Study

Objective. Kisspeptin 1 might reflect increased androgen level in polycystic ovarian syndrome instead of other markers. Study Design. A case control study was performed in Al-Yarmouk Teaching Hospital from 1st of July 2016 to 1st of July 2017; it involved 87 women divided into two groups: 44 women diagnosed as PCOS, 22 women with BMI ≥ 25 kg/m2 and 22 women with BMI < 25 kg/m2, and another 43 women without PCOS, 22 women with BMI ≥ 25 kg/m2 and 21 women with BMI < 25 kg/m2. Hormonal, metabolic profiles, and hirsutism scores, as well as serum kisspeptin level, were assessed by using Human Kisspeptin 1(KISS-1) ELISA Kit. The blood samples between days 2 and 5 of menstrual cycle were drawn by an disposable sterile syringe and collected in EDTA containing tubes (as anticoagulant), and the hormonal profile was measured using a biotech ELISA reader. Result. Serum level of kisspeptin was significantly higher in PCOS compared to control (322.4 vs. 235.3 ng/L, respectively). There was no significant difference in age, BMI, and parity between control and PCOS; the frequency of hirsutism, acne, elevated LH, and increased free testosterone (fTT) were significantly higher in PCOS compared to control. Kisspeptin shows a direct significant correlation with hirsutism and fTT (r = 0.648, 0.238, respectively). In ROC analysis, kisspeptin had AUC (95% CI) = 0.874 (0.785–0.935) for predicting PCOS. Conclusion. Kisspeptin levels might be used as a marker for hyperandrogenemia in polycystic ovarian syndrome.

AMH levels in girls with various pubertal problems

2017 ◽  
Vol 30 (3) ◽  
Author(s):  
Senay Savas-Erdeve ◽  
Elif Sagsak ◽  
Meliksah Keskin ◽  
Semra Cetinkaya ◽  
Zehra Aycan
Keyword(s):  
Standard Deviation Score ◽  
Central Precocious Puberty ◽  
Primordial Follicle ◽  
Free Testosterone ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Premature Thelarche ◽  
And Control

AbstractBackground:Anti-Müllerian hormone (AMH) is produced by granulosa cells surrounding follicles that have undergone recruitment from the primordial follicle pool but have not been selected for dominance (preantral and early antral follicles). In healthy girls, serum levels of AMH vary considerably between individuals. We aimed to evaluate the AMH level in girls with premature thelarche (PT) and central precocious puberty (CPP).Methods:Girls with CPP (n=21), PT (n=24) and a control prepubertal group (n=22) were included in the study.Results:AMH levels were significantly higher in the PT group than the prepubertal control group and similar to the CPP group. AMH levels in the CPP group were similar to the prepubertal control group. AMH levels showed a significant negative correlation with luteinizing hormone (LH), free testosterone and dehydroepiandrosterone sulphate (DHEAS) levels in the PT group. AMH levels were negatively correlated with height standard deviation score (HSDS), body mass index (BMI) SDS values and positively correlated with sex hormone binding globulin (SHBG) levels in the CPP group. These levels were positively correlated with SHBG levels in the control prepubertal group.Conclusions:Serum AMH levels in girls with PT was found to be higher than in prepubertal girls. AMH levels in the CPP group were not different compared with the PT and control groups.

Adiponectin and leptin levels in normal weight women with polycystic ovary syndrome

2020 ◽  
Vol 41 (4) ◽  
Author(s):  
Murat Gözüküçük ◽  
Aslı Yarcı Gürsoy ◽  
Emre Destegül ◽  
Salih Taşkın ◽  
Hakan Şatıroğlu
Keyword(s):  
Insulin Resistance ◽  
Polycystic Ovary ◽  
Statistical Significance ◽  
Free Testosterone ◽  
Normal Weight ◽  
Control Group ◽  
Study Group ◽  
Model Assessment ◽  
Homeostatic Model Assessment ◽  
The Difference

Abstract Objectives Since polycystic ovarian syndrome (PCOS) is prevalent in reproductive women with obesity and insulin resistance, adipocytokines are often accused and investigated for pathophysiology. The aim of this study was to evaluate the adiponectin and leptin levels in normal-weight women with PCOS. Methods Forty women with PCOS and 40 age and body mass index (BMI) matched controls were included in the study. Adiponectin and leptin levels in addition to other biochemical parameters were measured. Results Leptin levels were statistically significantly higher in the study group compared to the control group (6.53 ± 2.670 vs 3.37 ± 2.002 ng/mL, p < 0.001 respectively). Although Adiponectin levels were lower in the study group compared to the control group (28.89 ± 16.124 μg/mL vs 31.05 ± 20.507, p = 0.714 respectively) the difference did not reach statistical significance. Leptin levels were positively correlated with fasting glucose, fasting insulin, free testosterone levels and homeostatic model assessment of insulin resistance (HOMA-IR) values. Adiponectin levels were negatively correlated with BMI. Conclusions Adiponectin and leptin have been suggested to play a crucial role in the pathogenesis of PCOS. Different adipocytokine levels in the normal weight PCOS group compared to age and BMI matched controls support the idea that adipose tissue in this group of women has some distinctive features not only in high BMI subgroup but also in normal weight subgroup.

scholarly journals Menstrual cycle disorders in polycystic ovary syndrome: Ultrasonographic characteristics and hormonal status

2002 ◽  
Vol 55 (5-6) ◽  
pp. 241-246
Author(s):  
Aleksandar Radulovic ◽  
Mirjana Bogavac ◽  
Aleksandra Trninic-Pjevic
Keyword(s):  
Menstrual Cycle ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Normal Weight ◽  
Age At Menarche ◽  
Hormonal Status ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Cycle Disorders ◽  
Pco Syndrome

Introduction The polycystic ovary syndrome is primarily characterized by an association of hyperandrogenism and anovulation. The aim was to determine the incidence of menstrual cycle disorders in patients with PCO syndrome and the relation with ultrasonographic and hormonal findings. Material and methods A retrospective study examined the ultrasonographic findings and hormonal status in patients with polycystic ovary syndrome at the Department of Obstetrics and Gynecology of the General Hospital in Subotica. The sample comprised 39 patients. Results and conclusions Most frequently age at menarche was 14 years (29%) in obese and 10 years (45.5%) in normal weight patients. There was not a significant difference in duration of menstrual bleeding in both groups of examined patients, whereas amenorrhoea was significantly more frequent in obese patients (29.4%) than in patients with normal weight (4.7%). On the basis of presented results (increased percentage of severe disorders of menstrual cycle in all patients in subgroup C and increased percentage of menstrual disorders in patients with high stromal density in relation to those with normal echogenity of stroma) it can be concluded that there is a correlation between ultrasonographic findings of ovaries (number of microcysts, volume and density of the stroma) and menstrual cycle disorders, the polycystic ovary syndrome.

scholarly journals Ultrasonographic and biochemical assessments as early prediction of polycystic ovarian syndrome in obese women

2020 ◽  
Vol 20 (2) ◽  
pp. 676-681
Author(s):  
Alaa A Ahmed ◽  
Said S Moselhy ◽  
Taha A Kumosani ◽  
Etimad A Huwait ◽  
Maryam A AL-Ghamdi ◽  
...  
Keyword(s):  
Biochemical Markers ◽  
Polycystic Ovary ◽  
Glycosylated Hemoglobin ◽  
Clinical Signs ◽  
Free Testosterone ◽  
Early Prediction ◽  
Obese Women ◽  
Ovary Syndrome ◽  
Ovarian Morphology ◽  
Ovarian Syndrome

Backgroud: Polycystic ovary syndrome (PCOS) is considered as a common cause of hormonal disturbance and obesity. The diagnosis of PCOS was done by different methods including clinical signs as anovulation, hyperandrogenism, biochem- ical markers and ultrasounographic investigation. This study investigated comparative outcomes of ultrasonographic and biochemical markers for early prediction of PCOS in obese women. Subjects and methods: Seventy-five patients were clinically diagnosed with obese, PCOS and obese with PCOS and twen- ty-five normal age matched subjects were enrolled as control. Abdominal and transvaginal ultrasonographic for assessment of ovarian properties. In addition, BMI, serum free testosterone, dehydroepiandrosterone (DHEA), insulin, glycosylated hemoglobin (HbA1c) and LDL-c levels were evaluated. Results: In obese patients with PCOs (20%) ovaries revealed normal appearance in morphology while the rest (80%) showed PCOs in the form of cysts of 2–8 mm in diameter peripherally arranged around stroma. A significant elevation of free testosterone, DHEA and insulin in obese with or without PCOS compared with obese group (p<0.001). A positive correlation with hormonal abnormalities of increased HA1c, LDL-c, free testosterone, DHEA and insulin compared with obese only. Conclusion: According to our study findings, ovarian morphology combined with biochemical markers is more reliable for early prediction and diagnosis of PCOS for interpretation and management. Keywords: PCOS; ultrasound; diagnosis; hormones.

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