scholarly journals Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhiqiang Xu ◽  
Yuqi He ◽  
Xiufeng Zeng ◽  
Xiuxia Zeng ◽  
Junhui Huang ◽  
...  
Keyword(s):  
Dental Implants ◽  
Porphyromonas Gingivalis ◽  
Soft Tissues ◽  
Titania Nanotubes ◽  
Biological Behavior ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Related Gene ◽  
Gene Expressions ◽  
Tissue Integration

Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants.

Dopamine self-polymerized along with hydroxyapatite onto the preactivated titanium percutaneous implants surface to promote human gingival fibroblast behavior and antimicrobial activity for biological sealing

2018 ◽  
Vol 32 (8) ◽  
pp. 1071-1082 ◽  
Author(s):  
Minggang Yang ◽  
Peipei Jiang ◽  
Yang Ge ◽  
Fang Lan ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Soft Tissue ◽  
Titanium Surface ◽  
Soft Tissues ◽  
Pure Titanium ◽  
Carboxymethyl Chitosan ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Tissue Integration ◽  
Collagen Secretion

The clinical success of dental implants requires not only the optimum osseointegration but also the integration of implant surface with soft tissues to form biological sealing. In this study, alkali-heat treatment was applied to modify the pure titanium surface constructing a unique micro-and nano-structure. Then, poly(dopamine), along and with the additional incorporation of hydroxyapatite and carboxymethyl chitosan have been successfully infiltrated into the preactivated Ti surface during dopamine self-polymerization proceeding. Here, the effects of poly(dopamine)-modified surface coating on the biological behaviors of human gingival fibroblasts (HGFs) and oral pathogens have been systematically studied, which was critical for the early peri-implant soft tissue integration. The results showed that the poly(dopamine)-modified alkali-heat-titanium surface was a superior substrate for human gingival fibroblast adhesion, spread and proliferation. Moreover, further enhancements on cytoskeleton organization, collagen secretion and fibronectin adsorption were generally observed through the additional incorporation of hydroxyapatite. The addition of carboxymethyl chitosan exerted a positive modulation effect on antibacterial activity. Overall, our study demonstrated that combined superior soft tissue integration and antibacterial activity can be achieved by using poly(dopamine)-modified titanium implant, which has great potential in the optimal design of dental implant.

Porphyromonas gingivalis Lipopolysaccharide Induces a Pro-inflammatory Human Gingival Fibroblast Phenotype

Inflammation ◽  
2016 ◽  
Vol 40 (1) ◽  
pp. 144-153 ◽  
Author(s):  
S. Buket Bozkurt ◽  
Sema S. Hakki ◽  
Erdogan E. Hakki ◽  
Yusuf Durak ◽  
Alpdogan Kantarci
Keyword(s):  
Porphyromonas Gingivalis ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Porphyromonas Gingivalis Lipopolysaccharide

scholarly journals Clinical study on the gingival and osseointegration of some medical devices from dental implant category at the level of the oral cavity – preliminary analysis (part I)

2021 ◽  
Vol 67 (1) ◽  
pp. 35-39
Author(s):  
Teodora Marcu ◽  
◽  
Augustin Mihai ◽  
Mihai Burlibasa ◽  
Corina Marilena Cristache ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Dental Implants ◽  
Dental Implant ◽  
Medical Devices ◽  
Soft Tissues ◽  
Tissue Integration ◽  
Tissue Changes ◽  
One Year ◽  
Serious Injuries

Introduction. In order to achieve the goals of oral implantology, it is of great importance to obtain tissue integration (bone and epithelial) of dental implants. Bone integration has the role of rigidly fixing the dental implant at the level of the supporting bone, while ensuring the transfer of masticatory forces to the neighboring bone, while the soft tissues integration has the role of creating a barrier around these medical devices, with the role of protecting the underlying bone and preventing the access of microorganisms. Purpose. In this material we wanted to perform a statistical analysis on the gingival integration and osseointegration of dental implants in the oral cavity. Material and method. The analysis was performed on a number of 21 patients with a total of 97 dental implants, approximately one year after the prosthetic loading of the implants. The analysis was based on bleeding index, gingival index but also on the depth of the peri-implant canal and bone resorption around the implant. Results and discussions. Most of the implants analyzed showed no signs of inflammation. A small number (less than 30%) showed incipient signs of inflammation, falling within the data in the literature. Conclusions. Early detection of tissue changes around the implant and proper treatment will reduce the risk of more serious injuries and implant failure.

scholarly journals RETRACTED ARTICLE: Temporal activation of anti- and pro-apoptotic factors in human gingival fibroblasts infected with the periodontal pathogen, Porphyromonas gingivalis: potential role of bacterial proteases in host signalling

2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Sonya Urnowey ◽  
Toshihiro Ansai ◽  
Vira Bitko ◽  
Koji Nakayama ◽  
Tadamichi Takehara ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Pi3 Kinase ◽  
Bacterial Invasion ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Nf Kappa B ◽  
Proteolytic Activation ◽  
Temporal Shift ◽  
Apoptotic Genes

Abstract Background Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. Results We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) cells. Quantitative immunostaining revealed the need for a high multiplicity of infection for optimal infection. Early in infection (2–12 h), P. gingivalis activated the proinflammatory transcription factor NF-kappa B, partly via the PI3 kinase/AKT pathway. This was accompanied by the induction of cellular anti-apoptotic genes, including Bfl-1, Boo, Bcl-XL, Bcl2, Mcl-1, Bcl-w and Survivin. Late in infection (24–36 h) the anti-apoptotic genes largely shut down and the pro-apoptotic genes, including Nip3, Hrk, Bak, Bik, Bok, Bax, Bad, Bim and Moap-1, were activated. Apoptosis was characterized by nuclear DNA degradation and activation of caspases-3, -6, -7 and -9 via the intrinsic mitochondrial pathway. Use of inhibitors revealed an anti-apoptotic function of NF-kappa B and PI3 kinase in P. gingivalis-infected HGF cells. Use of a triple protease mutant P. gingivalis lacking three major gingipains (rgpA rgpB kgp) suggested a role of some or all these proteases in myriad aspects of bacteria-gingival interaction. Conclusion The pathology of the gingival fibroblast in P. gingivalis infection is affected by a temporal shift from cellular survival response to apoptosis, regulated by a number of anti- and pro-apoptotic molecules. The gingipain group of proteases affects bacteria-host interactions and may directly promote apoptosis by intracellular proteolytic activation of caspase-3.

scholarly journals Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2799
Author(s):  
Aya Q. Alali ◽  
Abdalla Abdal-hay ◽  
Karan Gulati ◽  
Sašo Ivanovski ◽  
Benjamin P. J. Fournier ◽  
...  
Keyword(s):  
Dental Implants ◽  
Metabolic Activity ◽  
Matrix Protein ◽  
Clinical Importance ◽  
Titanium Implants ◽  
Extracellular Matrix Protein ◽  
Gingival Fibroblast ◽  
Mrna Levels ◽  
Tissue Integration

Soft tissue integration (STI) at the transmucosal level around dental implants is crucial for the long-term success of dental implants. Surface modification of titanium dental implants could be an effective way to enhance peri-implant STI. The present study aimed to investigate the effect of bioinspired lithium (Li)-doped Ti surface on the behaviour of human gingival fibroblasts (HGFs) and oral biofilm in vitro. HGFs were cultured on various Ti surfaces—Li-doped Ti (Li_Ti), NaOH_Ti and micro-rough Ti (Control_Ti)—and were evaluated for viability, adhesion, extracellular matrix protein expression and cytokine secretion. Furthermore, single species bacteria (Staphylococcus aureus) and multi-species oral biofilms from saliva were cultured on each surface and assessed for viability and metabolic activity. The results show that both Li_Ti and NaOH_Ti significantly increased the proliferation of HGFs compared to the control. Fibroblast growth factor-2 (FGF-2) mRNA levels were significantly increased on Li_Ti and NaOH_Ti at day 7. Moreover, Li_Ti upregulated COL-I and fibronectin gene expression compared to the NaOH_Ti. A significant decrease in bacterial metabolic activity was detected for both the Li_Ti and NaOH_Ti surfaces. Together, these results suggest that bioinspired Li-doped Ti promotes HGF bioactivity while suppressing bacterial adhesion and growth. This is of clinical importance regarding STI improvement during the maintenance phase of the dental implant treatment.

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