scholarly journals Effect of Steam Blanching, Dehydration Temperature & Time, on the Sensory and Nutritional Properties of a Herbal Tea Developed from Moringa oleifera Leaves

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yasara W. H. Wickramasinghe ◽  
Indira Wickramasinghe ◽  
Isuru Wijesekara
Keyword(s):  
Moringa Oleifera ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Herbal Tea ◽  
Nutritional Properties ◽  
Phenolic Contents ◽  
Trained Panel ◽  
Two Samples ◽  
Moringa Oleifera Leaves ◽  
Dpph Scavenging

The core purpose of the current study is to explore the use of Moringa oleifera leaves, to produce a herbal tea with acceptable sensory properties and nutritional properties by utilizing the steam blanching technique, different dehydration temperatures and time, which can be accepted in the Sri Lankan market. Six sets of samples were prepared where temperature and time combinations were 55°C—6 h, 60°C—4.30 h, 65°C—3 h for the unblanched samples & 55°C—6 h, 60°C—5.30 h & 65°C—4 h for the steam blanched samples. These samples were evaluated, employing a trained panel of 5 tea tasters and a semi trained panel of 35 members. The sample code 706 (steam blanched, 65°C—4 h) was selected as the sample with best sensory attributes. The blanched and unblanched samples dried at 65°C were tested for their proximate, mineral, vitamin, antioxidant and phytochemical contents. The effects of steam blanching on these two samples were evaluated & compared. This study highlights that steam blanching significantly increased the carbohydrates, fat, Mn, Fe, vitamin A, vitamin E and the DPPH scavenging activity whereas steam blanching significantly reduced the protein, fiber, Na, K, Ca, Total phenolic contents and flavonoids content but vitamin C, Zn, Cu and Mg contents were unaffected by steam blanching.

scholarly journals Antioxidant and Antidiabetic Potential of Saponin Fraction Isolated from Moringa oleifera Leaves

2020 ◽  
Vol 63 (2) ◽  
pp. 86-92
Author(s):  
Fatma Hussain ◽  
Fatima Ikram
Keyword(s):  
Moringa Oleifera ◽  
Dry Weight ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Dpph Scavenging Activity ◽  
Saponin Fraction ◽  
Dpph Scavenging ◽  
Layer Chromatography ◽  
Bio Assay

To assess total phenolic contents, total flavonoid contents (TPC, TFC), DPPH scavenging and antidiabetic (antiglycation and alpha amylase hindrance) activities of Moringa oleifera (MO) leaves, bio- assay guided fractionation was used with thin layer chromatography profile to isolate saponin fraction (SF). The TPC and TFC were 9.7-12.8 GAE and 5.3-9.3 CE (g/100 g dry weight), respectively and aqueous and methanol fractions were more effective. DPPH scavenging activity ranged 69.5-83.1%. SF displayed noteworthy antioxidant ability (p<0.046) and it was leading inhibitor of glycation (p<0.041) after MeOH with 48.1% restriction. Enzyme inhibition by MO fractions was negligible (3.4-9.2%) and it was less impressive by SF as compared to MeOH extract. Percent reduction in enzyme activity by SF was non- significant (p>0.198), nullifying its concentration dependent impact.    

scholarly journals Effects of Adding Moringa oleifera Leaves Powder on the Nutritional Properties, Lipid Oxidation and Microbial Growth in Ground Beef during Cold Storage

2021 ◽  
Vol 11 (7) ◽  
pp. 2944
Author(s):  
Mpho Edward Mashau ◽  
Kgaogelo Edwin Ramatsetse ◽  
Shonisani Eugenia Ramashia
Keyword(s):  
Lipid Oxidation ◽  
Cold Storage ◽  
Moringa Oleifera ◽  
Microbial Growth ◽  
Ground Beef ◽  
Total Phenolic ◽  
Microbial Composition ◽  
Nutritional Properties ◽  
Cooking Properties ◽  
Moringa Oleifera Leaves

The utilisation of Moringa oleifera leaves powder (MOLP) to improve the nutritional properties and inhibit lipid oxidation and the proliferation of microorganisms in ground beef during cold storage was examined. The effects of 0.2, 0.4, 0.6, and 0.8% MOLP on the nutritional properties (proximate composition, total phenolic and total flavonoid content), thiobarbituric acid reactive substances (TBARS), microbial composition, physicochemical characteristics (pH value, colour attributes, and cooking properties), and sensory analysis of ground beef were investigated. The findings showed that ash, protein, polyphenolic compounds, pH, colour, and microbial growth increased significantly, while moisture, fat content, and TBARS decreased significantly, with an increase in the concentration of MOLP during cold storage. Moderate levels (0.2 and 0.4%) of MOLP did not affect the sensory attributes of stored ground beef. Evidently, MOLP can be utilised as a natural preservative in ground beef to improve the nutritional value and inhibit lipid oxidation.

scholarly journals Green Synthesis of Encapsulated Copper Nanoparticles Using a Hydroalcoholic Extract of Moringa oleifera Leaves and Assessment of Their Antioxidant and Antimicrobial Activities

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 555 ◽  
Author(s):  
Prince Edwin Das ◽  
Imad A. Abu-Yousef ◽  
Amin F. Majdalawieh ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Electron Microscopy ◽  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Moringa Oleifera ◽  
Metallic Copper ◽  
Total Phenolic ◽  
Copper Sulphate Solution ◽  
Hydroalcoholic Extract ◽  
Moringa Oleifera Leaves ◽  
Anti Fungal

The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. The medicinal value of Moringa oleifera leaves and the antimicrobial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable added-value inorganic material. The use of a hydroalcoholic extract of M. oleifera leaves for the green synthesis of copper nanoparticles is an attractive method as it leads to the production of harmless chemicals and reduces waste. The total phenolic content in the M. oleifera leaves extract was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. The M. oleifera leaves extract was treated with a copper sulphate solution. A color change from brown to black indicates the formation of copper nanoparticles. Characterization of the synthesized copper nanoparticles was performed using ultraviolet-visible light (UV-Vis) spectrophotometry, Fourier-transform infrared (FTIR) spectrometry, high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The synthesized copper nanoparticles have an amorphous nature and particle size of 35.8-49.2 nm. We demonstrate that the M. oleifera leaves extract and the synthesized copper nanoparticles display considerable antioxidant activity. Moreover, the M. oleifera leaves extract and the synthesized copper nanoparticles exert considerable anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis (MIC values for the extract: 500, 250, 250, and 250 µg/mL; MIC values for the copper nanoparticles: 500, 500, 500, and 250 µg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized copper nanoparticles exert relatively stronger anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (MIC values for the extract: 62.5, 62.5, 125, and 250 µg/mL; MIC values for the copper nanoparticles: 125, 125, 62.5, and 31.2 µg/mL, respectively). Our study reveals that the green synthesis of copper nanoparticles using a hydroalcoholic extract of M. oleifera leaves was successful. In addition, the synthesized copper nanoparticles can be potentially employed in the treatment of various microbial infections due to their reported antioxidant, anti-bacterial, and anti-fungal activities.

scholarly journals Production of instant coffee from cold brewed coffee; process characteristics and optimization

2020 ◽  
Vol 3 (1) ◽  
pp. 39
Author(s):  
Patroklos K Vareltzis ◽  
Marina Zeleskidou ◽  
Smaro Kiroglou ◽  
Irene Gargali
Keyword(s):  
Physicochemical Characteristics ◽  
Total Phenolic ◽  
Inlet Temperature ◽  
Instant Coffee ◽  
Process Characteristics ◽  
Coffee Beans ◽  
Trained Panel ◽  
Anti Oxidant ◽  
Dpph Scavenging ◽  
Drying Conditions

This study aimed to develop a process for producing instant coffee from cold brewed Arabica and Robusta roasted coffee beans. The process starts by selecting an appropriate mixture of Arabica and Robusta coffee beans, degree of roasting (light, medium, dark) and degree of grinding (coarse, fine). Sensory evaluation by a trained panel showed that a mixture of 92.5% Arabica and 7.5% Robusta medium roasted, coarsely ground coffee beans produces instant cold brewed coffee equally or better accepted from other instant coffees available to consumers. The selected coffee beans were subjected to vacuum assisted cold extraction and the extract was spray dried. Spray drying conditions (inlet temperature, air volumetric flow and air aspiration rate) were optimized by a 33 full factorial design. Response variables were solids yield, process thermal efficiency and anti-oxidant capacity of the produced powder measured as %DPPH scavenging ability, FRAP and total phenolic content. The produced powder was analysed for its physicochemical characteristics (caffeine content, phenolics, moisture content, hygroscopicity, bulk density, solubility, particle size distribution, acrylamide content and shelf life determination).

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

scholarly journals The Influence of Solvent, Host, and Phenological Stage on the Yield, Chemical Composition, and Antidiabetic and Antioxidant Properties of Phragmanthera capitata (Sprengel) S. Balle

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Césaire Feudjio ◽  
Muhammad Arfat Yameen ◽  
Guy Sedar Singor Njateng ◽  
Muhammed Ahsan Khan ◽  
Stephen Lacmata Tamekou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Psidium Guajava ◽  
Biological Properties ◽  
Persea Americana ◽  
Total Phenolic ◽  
Phenological Stage ◽  
Phenolic Contents ◽  
Hplc Profile ◽  
Dpph Scavenging

Phragmanthera capitata was reported to possess many biological properties making it a good candidate for the formulation of a phytomedicine with multiple effects. In this work, we studied some factors likely to modify these therapeutic properties with the aim to contribute to its standardization as an improved traditional medicine. P. capitata parasitizing Persea americana, Psidium guajava, and Podocarpus mannii were harvested at three phenological stages (vegetative, flowering, and fruiting stages). The extracts were prepared by maceration in n-hexane, ethyl acetate, ethanol, methanol, and distilled water. The total phenolic, flavonoid, flavonol, and tannin contents were measured using appropriate methods. The antioxidant potential of extracts was investigated using TAC, DPPH scavenging, and FRAP methods. The α-amylase and α-glucosidase inhibitory activities of extracts were determined using enzymatic methods. The ethyl acetate extracts with the best phenolic content were subjected to HPLC analysis. The extraction yields were higher with methanol. The ethyl acetate extract of P. capitata harvested from P. guajava showed a stable HPLC profile during the development of the plant, while extracts from the plant collected from P. americana and P. mannii showed both qualitative and quantitative variations according to phonological stages of the plant. The inhibition of α-amylase was more pronounced for P. capitata harvested from P. guajava, decreasing during flowering and fruiting, while inhibition of α-glucosidase was not influenced by the phenological stage and the host of the plant. The α-amylase inhibitors were better extracted by ethyl acetate and those of α-glucosidase by ethanol or methanol. The phenolic contents and antioxidant properties of the extracts were influenced by the phenological stage of P. capitata and its hosts. These results suggest that it is preferable to harvest P. capitata during flowering or during fruiting stages on any host. None of the used solvents permitted an optimal extraction of active principles form P. capitata, suggesting that the mixture of solvents must be considered in further studies.

Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices / Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Mahfuz Elmastaş ◽  
İsa Telci ◽  
Hüseyin Akşit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Herbal Tea ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Antioxidant Agents

AbstractObjective: Mint (Mentha spp.) genotypes used as spices are cultivated in Turkey and used for different purposes including herbal tea, spices, the cosmetics industry, and are used in folk medicine. While mint species have been used in traditional practices during humanity’s long history, there is limited research on the comparison of their antioxidant capacity and phenolic contents. This aim of the research is to compare antioxidant capacity and phenolic contents in mint clones to determine superior genotypes for herbal and spice usage and cultivation.Methods: Antioxidant capacity was evaluated by ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC). Total phenolic content in clones were determined by Folin-Ciocalteu method.Results: Maximum total phenolic content [28.27±3.95 μg gallic acid equivalent (GAE)/g dry weight (DW)] and FRAP activity 577.09±46.02 μmol Trolox equiv/g DW were obtained from Clone 13 (M. spicata), while M. piperita clones were higher for TEAC activity (800.02±1.10 μmol Trolox equiv/g DW). The results were first records for M. villoso nervata. FRAP and TEAC activities selected clones were correlated with total phenolic content (r=0.77; 0.73 respectively).Conclusion: According to the results, it can be suggested that Clone 13, Clone 5, (M. spicata) Clone 3 and Clone 8 (M. piperita) are more suitable for use as spice, herbal tea, and antioxidant agents. The clones must be selected for commercial cultivation by the grower and they can be used as spices and herbal teas.

scholarly journals Moringa oleifera Supplementation Improves Antioxidant Status and Biochemical Indices by Attenuating Early Pregnancy Stress in Beetal Goats

2021 ◽  
Vol 8 ◽  
Author(s):  
Ali Afzal ◽  
Tarique Hussain ◽  
Amjad Hameed
Keyword(s):  
Early Pregnancy ◽  
Antioxidant Status ◽  
Moringa Oleifera ◽  
Basal Diet ◽  
Total Phenolic ◽  
Control Group ◽  
Phenolic Contents ◽  
Beneficial Effects ◽  
Blood Biochemical ◽  
Biochemical Indices

This study investigated the effects of supplementing different levels of Moringa oleifera leaf powder (MOLP) on antioxidant status and blood biochemical indices during early gestation in Beetal goats. A total of 30 goats were randomly divided into three groups (n = 10) comprising control (basal diet without MOLP), the 1.6% MOLP supplemented group (basal diet + 1.6% MOLP), and the 3.2% MOLP supplemented group (basal diet + 3.2% MOLP). The experiment started 5 days before estrus synchronization and lasted till day 60 of gestation. The MOLP significantly increased plasma flavonoids in 1.6% as well as 3.2% supplemented group on days 40 and 60 of pregnancy, while total phenolic contents were observed to be higher in the 3.2% MOLP supplemented group throughout the experiment in comparison with the control group. The supplementation improved plasma total antioxidant capacity (TAC) by decreasing malondialdehyde (MDA) and total oxidant status (TOS) in contrast to the control group. The activities of superoxide dismutase (SOD) and peroxidase (POD) were enhanced in both supplemented groups, whereas catalase (CAT) activity was noticed to be significantly high in the 3.2% MOLP supplemented group. The protein contents were significantly elevated with 1.6 and 3.2% supplementation levels from day 40 to day 60 of the experiment. Plasma sugar level, carotenoids, progesterone profile, and hydrolytic (protease and amylase) enzymes activities were improved only when supplemented with 3.2% MOLP. The findings suggest that supplementing with 3.2% MOLP provides beneficial effects on early pregnancy stress in Beetal goats.

scholarly journals The influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense (Schumach & Thonn) seeds

2020 ◽  
Vol 12 (2) ◽  
pp. 211-217
Author(s):  
Babatunde Oso ◽  
Ige Olaoye
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Property A ◽  
Piper Guineense ◽  
Phenolic Contents ◽  
Significant Difference ◽  
Dpph Scavenging

The study assessed the influence of varying cooking temperatures on the antioxidant properties of the aqueous extract of Piper guineense seeds. Different portions of the aqueous extract of P. guineense seeds were cooked at different temperatures which include 50 oC, 70 oC, and 90 oC for 10 minutes, while the remaining portion was allowed to stand for 10 minutes at room temperature of 29 oC and all were assessed for the evaluations of reducing power, radical scavenging capacities, and total phenolic contents. The results showed that there was a significant increase in the ferric reducing power at 90 oC, however, there was no significant difference between the raw and the cooked P. guineense seeds at 50 oC and 70 oC. In addition, the radical scavenging potential of the extract was highest at 70 oC, however, none of the cooked P. guineense seeds at the selected temperatures exhibited lower DPPH scavenging property. A similar trend was observed for the phenolic content of the extract with a significant reduction at 90 oC. The study suggests that cooking at 70 oC could enhance the antioxidant potentials of P. guineense seeds

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