scholarly journals Determination of Caffeine Content in Commercial Energy Beverages Available in Saudi Arabian Market by Gas Chromatography-Mass Spectrometric Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Mohammed Al-Bratty ◽  
Hassan A. Alhazmi ◽  
Zia ur Rehman ◽  
Sadique A. Javed ◽  
Waquar Ahsan ◽  
...  
Keyword(s):  
Adult Population ◽  
Health Effect ◽  
Energy Drinks ◽  
Energy Drink ◽  
Spectrometric Analysis ◽  
High Concentration ◽  
Caffeine Consumption ◽  
Caffeine Content ◽  
Validation Parameters

The popularity of energy beverages among young adult population is high. These drinks are claimed to boost energy and performance and contain high concentration of caffeine as one of the several ingredients. Discrepancies have been encountered by some of the previous studies between the actual quantity of caffeine present in the product and the amount mentioned on the label, making the determination of caffeine content in these drinks very important. Thus, in this study, we analyzed the caffeine concentration in most popular energy drinks available in Saudi Arabia. The energy drink samples (n = 9) were procured from retail outlets. Sample solutions were prepared in methanol and analyzed for caffeine content by GC-MS. Chromatographic parameters were optimized to achieve optimum resolution and various validation parameters were evaluated. The method was successfully applied for the quantification of caffeine in energy drinks by directly injecting the multifold diluted samples in methanol. The method was linear (r2 = 0.999) over a concentration range of 5–25 µg/mL, specific, precise (%RSD of peak area = 0.56–0.78), and accurate (%recovery = 99.3–101.2%). The amounts of caffeine determined were found in the range of 20.82–33.72 mg/100 mL (52.05–84.3 mg/pack). Results revealed that the amount of caffeine actually present in the tested drinks varied within ±10% range from the amount specified on the product labels. The amounts of caffeine detected in tested beverages were within the USFDA safe upper limit of per-day caffeine consumption, which has indicated that the consumption of one serving of energy drink is unlikely to produce any adverse health effect.

scholarly journals Determination of Caffeine and Taurine Contents in Energy Drinks by HPLC-UV

2016 ◽  
Vol 9 ◽  
pp. 66-73 ◽  
Author(s):  
Krishna Prasad Rai ◽  
Hasta Bahadur Rai ◽  
Santosh Dahal ◽  
Saroj Chaudhary ◽  
Suraj Shrestha
Keyword(s):  
Amino Acids ◽  
Pilot Study ◽  
Quantitative Determination ◽  
Physiological Responses ◽  
Alcoholic Beverage ◽  
Energy Drinks ◽  
Energy Drink ◽  
Young Generation ◽  
Caffeine Content

Energy drinks are non-alcoholic beverage intended to enhance the psycho-physiological responses in human, which is especially popular among young generation in Nepal. It is normally high caffeinated drink added with other ingredients such as carbohydrates, amino acids, B-group of vitamins etc. In this study, 10 brands of energy drink available in Nepalese markets were taken then analyzed for quantitative determination of Caffeine and Taurine by HPLC-UV method. From the result obtained, pH and TSS values of energy drinks were found in the range of 2.96-3.81 and 6.64-18.21 respectively. Likewise, the Caffeine and Taurine content in same samples were found in the range of not detected (ND) to 35.78 mg/100 ml and ND to 387.5 mg/100 ml respectively. Only the 6 samples out of 10 were confi rmed caffeine content as per claimed in label, while only 3 samples were confi rmed for Taurine content as per label claimed. Based on this pilot study, the majority of samples did not meet the label claims in term of Caffeine and Taurine, which apparently indicated the misbranding of such drinks. Since, there is no any regulation for such energy drinks in Nepal, it seems to be a great challenge for regulation of their safety and misbranding.

scholarly journals Rapid High-Performance Liquide Chromatographic Method for Quantitative Determination of Caffeine in Different Soft and Energy Drinks Available in Bangladesh

2021 ◽  
Vol 9 (3) ◽  
pp. 1081-1089
Author(s):  
Juthi Mirza ◽  
Masuda Sultana ◽  
Md. Esrafil ◽  
Shamoli Akter ◽  
Md. Jahangir Alam ◽  
...  
Keyword(s):  
High Performance ◽  
Food Additives ◽  
Reference Value ◽  
Energy Drinks ◽  
Energy Drink ◽  
Hplc Method ◽  
Soft Drinks ◽  
Caffeine Content ◽  
And Control

Caffeine is one of the commonly used food additives, which has unique flavor characteristics and bitter taste and used in soft drinks as flavor enhancer. An experimental study was designed to determine the concentration of caffeine in different brands of soft drinks and energy drinks available in Bangladesh by using HPLC. For chromatographic analysis, A Luna 5 C18 (2) 100A column (250×4.6 mm) was used at 37°C temperature at the wavelength of 272nm. Chromatographic separation was determined using buffer of sodium acetate and acetic acid with acetonitrile at a ratio of 80:20 (pH=4.0; flow rate of 1.0 ml/min). The results of this study showed that caffeine content in soft drinks ranged from 19.63 to 101.73 mg/100ml and highest concentration of caffeine found in brand 3 samples while lowest concentration found in brand 2 samples. Significantly higher concentration of caffeine (p<0.05) found in six soft drinks sample when compared to BSTI and FDA reference value except brand 2 sample (p>0.05). Quantification of caffeine in different brands of energy drink sample revealed that, four brand sample contained caffeine; among them brand 3 sample showed the highest levels of caffeine 295.86 mg/100ml and lowest amount found in brand 1 sample (101.74 mg/100ml). Concentration of Caffeine in soft and energy drinks exceeded the national and international standard recommended range hence this proposed HPLC method can be used for routine determination and control of caffeine content in different drinks.

scholarly journals Simultaneous Determination of Caffeine and Taurine in Energy Drinks by FT-MIR Spectroscopy Coupled with Multivariate Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Marcela González-Vázquez ◽  
Ofelia Gabriela Meza-Márquez ◽  
Tzayhri Gallardo-Velázquez ◽  
Guillermo Osorio-Revilla ◽  
José Luis Velázquez Hernández ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Fourier Transform ◽  
Standard Error ◽  
Energy Drinks ◽  
Mid Infrared ◽  
Determination Coefficient ◽  
Caffeine Content ◽  
Mir Spectroscopy ◽  
Validation Stage

Energy drinks have been studied due to their damaging side effects on the health of their consumers when consumed in excess or when combined with alcohol. Our objective was to develop chemometric models, based on Fourier-transform mid-infrared (FT-MIR) spectroscopy, to quantify the taurine and caffeine content in energy drinks rapidly and simultaneously. The taurine and caffeine content in the 50 samples ranged between 0 and 69.51 mg/100 mL and 14.92 and 1126.17 mg/100 mL, respectively. The best prediction model was obtained with the partial least squares (PLS1) algorithm; for taurine, the following values were obtained: determination coefficient of calibration (Rc2) = 0.9999, standard error of calibration (SEC) = 0.15, determination coefficient of validation (Rv2) = 0.9997, and standard error of prediction (SEP) = 0.16; for caffeine, Rc2 = 0.9999, SEC = 0.26, Rv2 = 0.9999, and SEP = 0.32. The model developed with PLS1 showed certainty in predictions during the validation stage and during application to external samples. FT-MIR coupled to chemometrics is a reliable and fast technique (compared to conventional techniques) to quantify taurine and caffeine in energy drinks simultaneously.

scholarly journals Determination of Caffeine Content in Non-Alcoholic Beverages and Energy Drinks Using Hplc-Uv Method.

2010 ◽  
Vol 9 (1) ◽  
Author(s):  
M Karau ◽  
J Kihunyu ◽  
N Kathenya ◽  
L Wangai ◽  
D Kariuki ◽  
...  
Keyword(s):  
Energy Drinks ◽  
Alcoholic Beverages ◽  
Caffeine Content

scholarly journals Risk of Energy Drink Consumption to Adolescent Health

2018 ◽  
Vol 13 (1) ◽  
pp. 22-25 ◽  
Author(s):  
Lyndsey D. Ruiz ◽  
Rachel E. Scherr
Keyword(s):  
Sleep Disturbances ◽  
Illicit Drugs ◽  
Energy Drinks ◽  
Energy Drink ◽  
High Energy ◽  
Caffeine Consumption ◽  
The Past ◽  
Energy Drink Consumption ◽  
Simple Carbohydrates ◽  
National Poison Data System

Energy drinks are beverages marketed to quickly increase alertness and performance of the consumer that typically contain relatively high quantities of caffeine, simple carbohydrates, and a mixture of additional ingredients. The carbohydrate sources, usually glucose and sucrose, found in the beverages supply the substrates needed for physiological energy, while the high caffeine content supplies the perceived energy through enhancing feelings of alertness during fatigued states. Although mean youth caffeine consumption as a whole has decreased over the past 2 decades, adolescent energy drink consumption has significantly increased in the past 10 years. High energy drink consumption of youth is concerning due to the range of reported adverse reactions attributed to excessive caffeine consumption, ranging from mild sleep disturbances to death. Reactions are severe enough to require reporting to the National Poison Data System and may even require emergency medical treatment. Studies have also shown that adolescents who consume energy drinks are likely to also use tobacco, alcohol, and illicit drugs. There is substantial evidence to suggest that the risk energy drinks pose to health are incredibly hazardous and should not be consumed by children and adolescents.

scholarly journals Energy Drinks and Their Adverse Health Effects: A Systematic Review and Meta-analysis

2020 ◽  
pp. 194173812094918
Author(s):  
Ibrahim M. Nadeem ◽  
Ajaykumar Shanmugaraj ◽  
Seaher Sakha ◽  
Nolan S. Horner ◽  
Olufemi R. Ayeni ◽  
...  
Keyword(s):  
Systematic Review ◽  
Adverse Effects ◽  
Adverse Events ◽  
Pediatric Population ◽  
Adult Population ◽  
Energy Drinks ◽  
Energy Drink ◽  
Control Group ◽  
Pertinent Data ◽  
Energy Drink Consumption

Context: Energy drinks are the fastest growing product in the beverage industry. However, there is concern regarding potential for adverse effects with use. Objective: To evaluate the reported adverse effects of energy drink consumption. Data Sources: The electronic databases MEDLINE, EMBASE, and PubMed were searched for relevant studies from inception to November 2019, and pertinent data were abstracted. Study Selection: Only clinical studies reporting adverse events after energy drink consumption were included. Study Design: Systematic review. Level of Evidence: Level 4. Data Extraction: Data regarding sample size characteristics, energy drink characteristics, comparators, and all adverse events were extracted in duplicate and recorded. Results: A total of 32 studies and 96,549 individuals were included. Frequently reported adverse events in the pediatric population were insomnia (35.4%), stress (35.4%), and depressive mood (23.1%). Frequently reported adverse events in the adult population were insomnia (24.7%), jitteriness/restlessness/shaking hands (29.8%), and gastrointestinal upset (21.6%). Alcohol mixed with energy drinks significantly reduced the likelihood of sedation effects but increased the likelihood of stimulatory effects. Energy drink consumption significantly increased the odds of insomnia (OR, 5.02; 95% CI, 1.72-14.63) and jitteriness/activeness (OR, 3.52; 95% CI, 1.28-9.67) compared with the control group. Conclusion: The authors recommend that individuals avoid frequent energy drink consumption (5-7 energy drinks/week) and avoid co-consumption with alcohol; increased regulatory standards should be placed in the sale of energy drinks, particularly with regard to the pediatric population.

scholarly journals A simple HPLC Method for the Determination of Caffeine Content in Tea and Coffee

2016 ◽  
Vol 9 ◽  
pp. 74-78 ◽  
Author(s):  
Prashanta Pokhrel ◽  
Suraj Shrestha ◽  
Som Kanta Rijal ◽  
Krishna Prasad Rai
Keyword(s):  
Dynamic Range ◽  
Hplc Method ◽  
Internal Diameter ◽  
Linear Dynamic Range ◽  
Uv Detector ◽  
Caffeine Content ◽  
Relative Standard ◽  
Validation Parameters ◽  
Correlation Coeffi

A simple HPLC-UV method was developed and validated for the determination of Caffeine content in tea and coffee samples of different local brands commercially available at Kathmandu and Kaski, Nepal. Water extracted caffeine was separated through c-18 column (ODS 5 μm, internal diameter 4.6 nm and length 150 mm) using methanol and water (40:60) as a mobile phase. The peak response time for caffeine was observed at 2.66 minutes using UV detector set at 275 nm. Method validation parameters viz. linearity, sensitivity (LOD & LOQ), repeatability and recovery were assessed for the detection of caffeine. Linear dynamic range was 2-10μg/ml with correlation coeffi cient (R2) of 0.9928. The LOQ and LOD were found to be 0.7 and 0.2μg/ml respectively. Relative standard deviation for retention time for intraday repeatability was 0.9 and for inter-day 1.63%. Recovery was measured by spiking blank tea samples and the results of recovery were greater than 97%. The validated method was fi nally adapted to determinate caffeine content in Nepalese tea and coffee samples. The average caffeine contents in tea and coffee were found in the range of (2.5-3.53) % and (1.17-1.34) % on dry basis respectively.

scholarly journals Spectrophotometric determining of caffeine content in the selection of teas, soft and energy drinks available on the Croatian market

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 325-330
Author(s):  
N. Vuletic ◽  
L. Bardic ◽  
R. Odzak
Keyword(s):  
Black Tea ◽  
Energy Drinks ◽  
Energy Drink ◽  
Soft Drinks ◽  
Caffeine Concentration ◽  
Beverage Samples ◽  
Caffeine Content ◽  
Carbonated Soft Drinks ◽  
Three Samples ◽  
Selection Of

This study aimed to determine concentrations of caffeine in the samples of the selected brands of teas, soft carbonated and energy drinks that are commercially available on the Croatian market. Quantitative analysis of caffeine content in the chosen beverage samples was done with UV/Vis spectrophotometric method. Chloroform was used as the solvent and concentrations of caffeine were measured at the wavelength of 274 nm for three samples of each of the selected beverages. Among the analyzed tea samples the highest caffeine concentration was found in Franck's Black Tea (1471.021 ppm) and the lowest in Naturavita's Green Tea with Ginger (588.138 ppm). Between the analyzed carbonated soft drinks, the highest caffeine concentration was measured in Fresh Cola (136.036 ppm) and the lowest in Sky Cola (48.198 ppm). The highest caffeine concentration in the analyzed energy drink samples was measured in Hell energy drink (394.670 ppm) while the lowest was in Coca Cola Energy drink (173.574 ppm). The results of this study gave preliminary information about caffeine levels in often consumed teas, carbonated soft drinks and energy drinks in Croatia. The caffeine content in the analyzed teas and drink samples in this study was under the allowed one except for Red Bull and Hell.

scholarly journals Quick and simultaneous determination of caffeine and taurine in beverages using UPLC-ESI-MS

2021 ◽  
Vol 12 (1) ◽  
pp. 18-22
Author(s):  
Mohd Aftab Alam ◽  
Rayan Saud Al-Arifi ◽  
Abdulaziz Abdullah Al-Qarni ◽  
Abdullah Shaya Al-Dosseri ◽  
Fahad Ibrahim Al-Jenoobi
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Energy Drinks ◽  
Solvent System ◽  
Energy Drink ◽  
Molecular Ions ◽  
Soft Drinks ◽  
Cone Voltage ◽  
Esi Ms

A rapid UPLC-ESI-MS method was developed for simultaneous determination of caffeine and taurine in beverages (energy drinks and soft drinks). The molecular ions of caffeine and taurine were identified in single ion recording mode at m/z 194.98 and 125.86, respectively. The mass spectrometer parameters were optimized as: capillary voltage 3.0 kV, cone voltage 35 V, extractor 3 V, RF Lens 0.1 V, source temperature 150 °C, desolvation temperature 350 °C, nitrogen 600 L/h, LMR1 7.9, HMR1 15.2, IE1 0.30. The mobile phase comprising methanol (0.1% formic acid) (A) and water (5 mM ammonium acetate) (B) was used in gradient mode. The mobile phase components A and B were pumped in 80:20 (v:v) ratio from 0-0.44 min, and then 100% of component A was pumped between 0.45-0.68 min, and at 0.69 min the composition was returned to 80:20 (v:v) ratio of A and B till 2.0 min. Caffeine and taurine were eluted at 0.46 and 0.43 min, respectively. The samples of energy drinks and soft drinks were diluted in a solvent system comprising methanol and water in 80:20 (v:v) ratio. Our investigations showed that soft drinks SD1 and SD2 have 88.8±4.2% and 110.7±3.6% (w:w) caffeine of their labeled claim. The caffeine content in energy drink brands ED1, ED2, ED3, and ED4 was 76.9±2.5, 65.6±3.4, 88.1±12.6, and 89.1±2.8% (w:w) of labeled claims, respectively. While taurine content in ED1, ED2, ED3, and ED4 was 86.5±8.4, 81.3±27.5, 101.9±4.8, and 97.1±0.3% (w:w) of labeled claim, respectively.

scholarly journals Determination of Caffeine and Sodium Benzoate in Both Imported and Locally Manufactured Energy Drinks Using HPLC and Spectrophotometer

2021 ◽  
Vol 910 (1) ◽  
pp. 012129
Author(s):  
Sirwan A. Mam Rashid ◽  
Shilan M. Abdulla ◽  
Bawan H. Najeeb ◽  
Shokhan H. Hamarashid ◽  
Omer A. Abdulla
Keyword(s):  
Sodium Benzoate ◽  
Energy Drinks ◽  
Energy Drink ◽  
The Other ◽  
Other Hand ◽  
Health Authorities ◽  
Actual Content ◽  
Food Legislation ◽  
Local Companies

Abstract Three groups of energy drinks have been examined for sodium benzoate and caffeine using HPLC and spectrophotometer methods. Those brands include original UK brand which coded (RE), another two brands of (RL and B) which are imported from Austria to local companies, and the rest produced locally which are coded (T, FB, S and FE). The results showed that all brands contain sodium benzoate at different concentrations. FB brand comes out on top by containing around 416mg/l which was far above the permitted level by FAO/WHO, on the other hand the RE brand contained the lowest amount of sodium benzoate when analysed using HPLC. Results of caffeine examination showed that all samples contained lower levels of caffeine as it is claimed in their label to contain 300mg/l, in contrary to sodium benzoate the lowest concentration of caffeine found in the FB brand which was 13.78mg/l and the highest level of caffeine was found in FE brand which was 208.95mg/l. From the obtained results it is clear that none of the energy drink brands declared the actual content of both caffeine and sodium benzoate which usually required by food legislation and law. Therefore, it is considered as mislabelling which is recommended that the health authorities in our country to subject the local producers and the importers to present the required information on the labels without misleading consumers. It is also recommended to educate the population and put restriction on the local shops and supermarkets not to sell energy drinks to individuals under 16 years old.

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