scholarly journals The Radioprotective Effect of Procaine and Procaine-Derived Product Gerovital H3 in Lymphocytes from Young and Aged Individuals

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Anca Ungurianu ◽  
Denisa Margina ◽  
Claudia Borsa ◽  
Cristina Ionescu ◽  
Gudrun von Scheven ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Chemical Compounds ◽  
Dna Strand Breaks ◽  
Peripheral Blood Mononuclear ◽  
Strand Breaks ◽  
Young Subjects ◽  
Living Organisms ◽  
Dna Strand

Ionizing radiation induces genomic instability in living organisms, and several studies reported an ageing-dependent radiosensitivity. Chemical compounds, such as scavengers, radioprotectors, and modifiers, contribute to reducing the radiation-associated toxicity. These compounds are often antioxidants, and therefore, in order to be effective, they must be present before or during exposure to radiation. However, not all antioxidants provide radioprotection. In this study, we investigated the effects of procaine and of a procaine-based product Gerovital H3 (GH3) on the formation of endogenous and X-ray-induced DNA strand breaks in peripheral blood mononuclear cells (PBMCs) isolated from young and elderly individuals. Interestingly, GH3 showed the strongest radioprotective effects in PBMCs from young subjects, while procaine reduced the endogenous amount of DNA strand breaks more pronounced in aged individuals. Both procaine and GH3 inhibited lipid peroxidation, but procaine was more effective in inhibiting mitochondria free radicals’ generation, while GH3 showed a higher antioxidant action on macrophage-induced low-density lipoprotein oxidation. Our findings provide new insights into the mechanisms underlying the distinct effects of procaine and GH3 on DNA damage.

Positive Association Between DNA Strand Breaks in Peripheral Blood Mononuclear Cells and Polyunsaturated Fatty Acids in Red Blood Cells From Women

2007 ◽  
Vol 59 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Audur Y. Thorlaksdottir ◽  
Jon J. Jonsson ◽  
Laufey Tryggvadottir ◽  
Gudrun V. Skuladottir ◽  
Anna L. Petursdottir ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Peripheral Blood Mononuclear Cells ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Positive Association ◽  
Dna Strand Breaks ◽  
Peripheral Blood Mononuclear ◽  
Strand Breaks ◽  
Blood Mononuclear Cells ◽  
Dna Strand

scholarly journals Suppression of Free Radical-Induced DNA Strand Breaks by Linoleic Acid and Low Density Lipoprotein in Vitro

2003 ◽  
Vol 26 (8) ◽  
pp. 1129-1134 ◽  
Author(s):  
Kazuyuki Hiramoto ◽  
Yoshinobu Yasuhara ◽  
Ken-ichi Sako ◽  
Kazuhiro Aoki ◽  
Kiyomi Kikugawa
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Dna Strand Breaks ◽  
Low Density ◽  
Strand Breaks ◽  
Dna Strand

scholarly journals Daily intake of heat-killed Lactobacillus plantarum L-137 improves inflammation and lipid metabolism in overweight healthy adults: a randomized-controlled trial

2019 ◽  
Vol 59 (6) ◽  
pp. 2641-2649 ◽  
Author(s):  
Yusuke Tanaka ◽  
Yoshitaka Hirose ◽  
Yoshihiro Yamamoto ◽  
Yasunobu Yoshikai ◽  
Shinji Murosaki
Keyword(s):  
Lipid Metabolism ◽  
Lactobacillus Plantarum ◽  
Mononuclear Cells ◽  
Controlled Trial ◽  
Low Density Lipoprotein ◽  
Daily Intake ◽  
Density Lipoprotein ◽  
Control Group ◽  
Double Blind ◽  
Peripheral Blood Mononuclear

Abstract Purpose The effects of heat-killed Lactobacillus plantarum L-137 (HK L-137) on inflammation and lipid metabolism were investigated in overweight volunteers. Methods One hundred healthy subjects with a body mass index from 23.0 to 29.9 (51 men and 49 women; mean age: 41.4 years) were enrolled in this randomized, double-blind, placebo-controlled, parallel group study. Subjects were randomly assigned to daily administration of a tablet containing HK L-137 (10 mg) or a placebo tablet for 12 weeks. Blood samples were collected every 4 weeks to measure biomarkers of lipid metabolism and inflammatory mediators. Results The percent change of concanavalin A-induced proliferation of peripheral blood mononuclear cells was significantly larger in the HK L-137 group than in the control group, similar to previous studies. The decreases of aspartate aminotransferase and alanine aminotransferase over time were significantly larger in the HK L-137 group than in the control group, as were the decreases of total cholesterol, low-density lipoprotein cholesterol, and the leukocyte count at one time point. These effects of HK L-137 were stronger in the subjects with higher C-reactive protein levels. Conclusions These findings suggest that daily intake of HK L-137 can improve inflammation and lipid metabolism in subjects at risk of inflammation.

scholarly journals Transcriptomic Profiles Reveal Downregulation of Low-Density Lipoprotein Particle Receptor Pathway Activity in Patients Surviving Severe COVID-19

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3495
Author(s):  
Ivan Vlasov ◽  
Alexandra Panteleeva ◽  
Tatiana Usenko ◽  
Mikhael Nikolaev ◽  
Artem Izumchenko ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Transcriptional Response ◽  
Cholesterol Homeostasis ◽  
Receptor Activity ◽  
Low Density ◽  
Peripheral Blood Mononuclear ◽  
Pathway Activity ◽  
Medical University

To assess the biology of the lethal endpoint in patients with SARS-CoV-2 infection, we compared the transcriptional response to the virus in patients who survived or died during severe COVID-19. We applied gene expression profiling to generate transcriptional signatures for peripheral blood mononuclear cells (PBMCs) from patients with SARS-CoV-2 infection at the time when they were placed in the Intensive Care Unit of the Pavlov First State Medical University of St. Petersburg (Russia). Three different bioinformatics approaches to RNA-seq analysis identified a downregulation of three common pathways in survivors compared with nonsurvivors among patients with severe COVID-19, namely, low-density lipoprotein (LDL) particle receptor activity (GO:0005041), important for maintaining cholesterol homeostasis, leukocyte differentiation (GO:0002521), and cargo receptor activity (GO:0038024). Specifically, PBMCs from surviving patients were characterized by reduced expression of PPARG, CD36, STAB1, ITGAV, and ANXA2. Taken together, our findings suggest that LDL particle receptor pathway activity in patients with COVID-19 infection is associated with poor disease prognosis.

scholarly journals Increased DNA strand breaks in mononuclear cells from patients with rheumatoid arthritis.

1992 ◽  
Vol 51 (1) ◽  
pp. 8-12 ◽  
Author(s):  
L L Bhusate ◽  
K E Herbert ◽  
D L Scott ◽  
D Perrett
Keyword(s):  
Rheumatoid Arthritis ◽  
Mononuclear Cells ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand

scholarly journals VSTM1 regulates monocyte/macrophage function via the NF-κB signaling pathway

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1513-1524
Author(s):  
Xiao-Fei Wang ◽  
En-Zhou ◽  
Dong-Jiu Li ◽  
Cheng-Yu Mao ◽  
Qing He ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Transmembrane Domain ◽  
Human Peripheral Blood ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cellular Migration ◽  
Inflammatory Factors ◽  
Control Group ◽  
U937 Cells ◽  
Peripheral Blood Mononuclear

Abstract Objective V-set and transmembrane domain-containing protein 1 (VSTM1) is negatively correlated with inflammation. However, its effect on atherosclerosis (AS) remains largely unexplored. In this study, we aimed to assess the effect of VSTM1 on the biological function of human peripheral blood mononuclear cells /macrophages stimulated by oxidized low-density lipoprotein (ox-LDL). Methods U937 cells were divided into three groups as follows: control group, pLenti-VSTM1 shRNA group (VSTM1 depletion), and pLenti-VSTM1 group (VSTM1 overexpression). Cellular migration, chemotaxis, apoptosis, and secretion of inflammatory factors of monocytes/macrophages stimulated by ox-LDL were studied. Results Overexpression of VSTM1 decreased the proliferation of U937 cells and induced cellular apoptosis. Depletion of VSTM1 enhanced the invasiveness and chemotaxis, increased the inflammatory response, and reduced the incidence of cell necrosis and apoptosis. Nuclear factor κ of B cells (NF-κB) was activated in VSTM1-depleted U937 cells. Conclusion VSTM1 might play an important role in the activation of monocytes/macrophages and participate in the pathogenesis of AS via regulating NF-κB activity.

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