scholarly journals Identification of Heat-Responsive Genes in Guar [Cyamopsis tetragonoloba (L.) Taub]

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Aref Alshameri ◽  
Fahad Al-Qurainy ◽  
Abdel-Rhman Gaafar ◽  
Salim Khan ◽  
Mohammad Nadeem ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Tolerance ◽  
Metabolic Pathways ◽  
Cyamopsis Tetragonoloba ◽  
Protein Families ◽  
New Genes ◽  
Key Genes

The threat of heat stress on crop production increased dramatically due to global warming leading to the rise on the demand of heat-tolerant crops and understanding their tolerance. The leguminous forage crop Guar [Cyamopsis tetragonoloba (L.) Taub] is a high-temperature tolerant plant with numerous works on its tolerance at morph-physiological levels but lack on molecular thermotolerance level. In the current study, the differential gene expression and the underlying metabolic pathways induced by heat treatment were investigated. An RNA-Seq study on Guar leaves was carried out to estimate gene abundance and identify genes involved in heat tolerance to better understand the response mechanisms to heat stress. The results uncovered 1551 up- and 1466 downregulated genes, from which 200 and 72 genes with unknown function could be considered as new genes specific to guar. The upregulated unigenes were associated with 158 enzymes and 102 KEGG pathways. Blast2GO, InterProScan, and Kyoto Encyclopaedia of Genes and Genomes packages were utilized to search the functional annotation, protein analysis, enzymes, and metabolic pathways and revealed hormone signal transduction were enriched during heat stress tolerance. A total of 301 protein families, 551 domains, 15 repeats, and 3 sites were upregulated and matched to those unigenes. A batch of heat-regulated transcription factor transcripts were identified using the PlantTFDB database, which may play roles in heat response in Guar. Interestingly, several heat shock protein families were expressed in response to exposure to stressful conditions for instance small HSP20, heat shock transcription factor family, heat shock protein Hsp90 family, and heat shock protein 70 family. Our results revealed the expressional changes associated with heat tolerance and identified potential key genes in the regulation of this process. These results will provide a good start to dissect the molecular behaviour of plants induced by heat stress and could identify the key genes in stress response for marker-assisted selection in Guar and reveal their roles in stress adaptation in plants.

scholarly journals Prospects of engineering thermotolerance in crops through modulation of heat stress transcription factor and heat shock protein networks

2014 ◽  
Vol 38 (9) ◽  
pp. 1881-1895 ◽  
Author(s):  
SOTIRIOS FRAGKOSTEFANAKIS ◽  
SASCHA RÖTH ◽  
ENRICO SCHLEIFF ◽  
KLAUS-DIETER SCHARF
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Networks

Temporal regulation of extracellular signal-regulated kinase 1/2 phosphorylation, heat shock protein 70 and activating transcription factor 3 during prostaglandin F-induced luteal regression in pseudopregnant rats following heat stress

2017 ◽  
Vol 29 (6) ◽  
pp. 1184 ◽  
Author(s):  
Wu-jiao Bai ◽  
Peng-jing Jin ◽  
Mei-qian Kuang ◽  
Quan-wei Wei ◽  
Fang-xiong Shi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Protein Kinase ◽  
Heat Shock Protein ◽  
Luteal Regression ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
Prostaglandin F ◽  
Activating Transcription Factor

The aim of the present study was to investigate the effects of heat stress on heat shock protein (HSP) 70 expression and mitogen-activated protein kinase (MAPK) and protein kinase (PK) B signalling during prostaglandin F (PGF)-induced luteal regression. During pseudopregnancy, rats were exposed to heat stress (HS, 40°C, 2 h) for 7 days and treated with PGF or physiological saline on Day 7; serum and ovaries were collected 0, 1, 2, 8 or 24 h after PGF treatment. The early inhibitory effect of PGF on progesterone was reduced in HS rats. HSP70 expression in response to PGF was significantly enhanced in HS rats. PGF-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was significantly greater in the HS group; however, HS rats exhibited elevated basal levels of phosphorylation of p38 MAPK, but not ERK1/2. PGF treatment increased expression of activating transcription factor (ATF) 3 at 2 h, which was inhibited by heat stress. Evaluating PKB signalling revealed that phosphorylation of p-Akt (Thr308 and Ser473) was reduced at 8 and 24 h after PGF treatment in both non-heat stress (NHS) and HS groups, but there were no significant differences between the HS and NHS groups at any of the time points. In conclusion, the present study provides further evidence that heat stress may enhance HSP70 and affect ERK1/2 and ATF3 expression, but not Akt activation, during PGF-induced luteal regression in pseudopregnant rats.

scholarly journals One Heat Shock Transcription Factor Confers High Thermal Tolerance in Clematis Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2900
Author(s):  
Rui Wang ◽  
Chanjuan Mao ◽  
Changhua Jiang ◽  
Long Zhang ◽  
Siyuan Peng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Heat Shock Transcription Factor ◽  
Heat Sensitivity ◽  
Resistance Response ◽  
Clematis Vitalba ◽  
Horticultural Plants ◽  
Heat Tolerant

Clematis plants play an important role in botanical gardens. Heat stress can destroy the activity, state and conformation of plant proteins, and its regulatory pathway has been well characterized in Arabidopsis and some crop plants. However, the heat resistance response mechanism in horticultural plants including Clematis has rarely been reported. Here, we identified a heat-tolerant clematis species, Clematis vitalba. The relative water loss and electrolytic leakage were significantly lower under heat treatment in Clematis vitalba compared to Stolwijk Gold. Differential expression heat-tolerant genes (HTGs) were identified based on nonparametric transcriptome analysis. For validation, one heat shock transcription factor, CvHSF30-2, extremely induced by heat stimuli in Clematis vitalba, was identified to confer tolerance to heat stress in Escherichia coli and Saccharomyces cerevisiae. Furthermore, silencing of HSF30-2 by virus-induced gene silencing (VIGS) led to heat sensitivity in tobacco and Clematis, suggesting that the candidate heat-resistant genes identified in this RNA-seq analysis are credible and offer significant utility. We also found that CvHSF30-2 improved heat tolerance of Clematis vitalba by elevating heat shock protein (HSP) expression, which was negatively regulated by CvHSFB2a. Taken together, this study provides insights into the mechanism of Clematis heat tolerance and the findings can be potentially applied in horticultural plants to improve economic efficiency through genetic approaches.

Role of heat stress response in the tolerance of immature renal tubules to anoxia

1998 ◽  
Vol 274 (6) ◽  
pp. F1029-F1036 ◽  
Author(s):  
Karen M. Gaudio ◽  
Gunilla Thulin ◽  
Andrea Mann ◽  
Michael Kashgarian ◽  
Norman J. Siegel
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Metabolic Response ◽  
Renal Tubules ◽  
Heat Stress Response

The stress response was studied in suspensions of tubules from immature (IT) and mature (MT) rats after noninjury, heat, oxygen, and anoxia. Under all conditions, IT exhibited more exuberant activation of heat shock transcription factor (HSF) than MT. Characterization of activated HSF in immature cortex revealed HSF1. Also, 2 h after each condition, heat shock protein-72 (HSP-72) mRNA was twofold in IT. As the metabolic response to 45 min of anoxia, 20-min reoxygenation was assessed by measuring O2 consumption (O2C). Basal O2C was manipulated with ouabain, nystatin, and carbonylcyanide p-chloromethyoxyphenylhydrazone (CCCP). Basal O2C in IT were one-half the value of MT. After anoxia, basal O2C was reduced by a greater degree in MT. Ouabain reduced O2C to half the basal value in both noninjured and anoxic groups. Basal O2C was significantly stimulated by nystatin but not to the same level following anoxia in MT and IT. Basal O2C was also stimulated by CCCP, but after anoxia, CCCP O2C was significantly less in MT with no decrease in IT, suggesting mitochondria are better preserved in IT. Also, O2C devoted to nontransport activity was better maintained in IT.

scholarly journals Transcriptomic and proteomic profiles of II YOU 838 (Oryza sativa) provide insights into heat stress tolerance in hybrid rice

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8306 ◽  
Author(s):  
Yan Wang ◽  
Yang Yu ◽  
Min Huang ◽  
Peng Gao ◽  
Hao Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Proteomic Analysis ◽  
Hybrid Rice ◽  
Expression Patterns ◽  
Mitogen Activated Protein Kinase ◽  
Transcription Activator ◽  
Antioxidant Defense Systems

Heat stress is an increasing threat to rice production worldwide. To investigate the mechanisms of heat tolerance in hybrid rice and their contributions to rice heterosis, we compared the transcriptome of the hybrid rice II YOU 838 (II8) with the transcriptomes of its parents Fu Hui 838 (F8) and II-32A (II3) after heat stress at 42 °C for 0 h, 24 h, 72 h and 120 h. We also performed a proteomic analysis in II8 after heat stress at 42 °C for 24 h. The transcriptome data revealed time-dependent gene expression patterns under the heat stress conditions, and the heat stress response of II8 was greatly different from those of its parents. Gene ontology analysis of the differentially expressed genes that were clustered using k-means clustering showed that most of the up-regulated genes were involved in responses to stimuli, cell communication, and metabolic and transcription factor activities, whereas the down-regulated genes were enriched in photosynthesis and signal transduction. Moreover, 35 unique differentially abundant proteins, including a basic helix-loop-helix transcription factor (bHLH96), calmodulin-binding transcription activator, heat shock protein (Hsp70), and chaperonin 60 (CPN60), were detected in the proteomic analysis of II8 under heat stress. The co-regulatory analysis revealed novel genes and pathways involved in heat tolerance, namely, ferredoxin-NADP reductase, peroxidases, mitogen-activated protein kinase kinase kinase, and heat shock factor (HSF)–Hsp network. Members of the Hsp and HSF families had over-dominant expression patterns in the hybrid compared with its parents, to help maintain the higher photosynthesis and antioxidant defense systems in the hybrid. Our study suggests that the complex HSF–Hsp regulatory network contribute to the heat tolerance of the hybrid rice.

scholarly journals Expression of heat shock protein (HSP) genes and antioxidant enzyme genes in hybrid rice II YOU 838 during heat stress

2021 ◽  
pp. 38-43
Author(s):  
Yan Wang ◽  
Min Huang ◽  
Peng Gao ◽  
Hao Chen ◽  
Yu Zheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Antioxidant Enzyme ◽  
Heat Tolerance ◽  
Hybrid Rice ◽  
High Temperature Stress ◽  
Flag Leaves

II YOU 838 (Oryza sativa subsp. indica), crossed by the maternal II-32A and paternal Fu Hui 838, was one of the most widely cultivated hybrid rice in China. Fu Hui 838, which has resistance to high temperature, was generated by mutation technology in 1990. Previous field-testing showed that II YOU 838 had tolerance to high temperature stress and this was confirmed in the present study. The mechanism of heat tolerance of II YOU 838 is not understood. The present study reports gene expression of a representative sample of heat-responsive proteins in II YOU 838 flag leaves subjected to heat stress during flowering. Differential expression of the heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), small heat shock protein (smHSP), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were studied under heat stress and optimum temperatures in flag leaves of II YOU 838. All six genes studied were responsive to high temperatures. Quantitative real-time PCR showed increased expression of the heat shock protein genes and antioxidant enzyme genes in flag leaves under heat stress. With increasing number of days gene expression decreased under high temperature. Peak expression of SOD, POD, hsp70 and hsp90 was on Day 2 under 39 ℃. On Day 3, the expression of CAT under 39 ℃ was the highest. The expression of smhsp was highest on Day 3 under 27 ℃, followed by that on Day 2 under 27 ℃. The maximum expression values were observed on Day 2 or Day 3 after beginning of heat stress. This suggests that hsp90, hsp70, SOD and POD are principally involved in early responses to heat in rice flag leaves, and that smhsp may play a role in the recovery mechanism in rice after heat stress. This may provide insights into the mechanism of heat-tolerance in rice

scholarly journals Activation of the DNA-binding ability of human heat shock transcription factor 1 may involve the transition from an intramolecular to an intermolecular triple-stranded coiled-coil structure.

1994 ◽  
Vol 14 (11) ◽  
pp. 7557-7568 ◽  
Author(s):  
J Zuo ◽  
R Baler ◽  
G Dahl ◽  
R Voellmy
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Heat Shock ◽  
Dna Binding ◽  
Hydrophobic Interactions ◽  
Coiled Coil ◽  
Heat Shock Transcription Factor ◽  
Single Amino Acid ◽  
Binding Ability ◽  
Heat Shock Element

Heat stress regulation of human heat shock genes is mediated by human heat shock transcription factor hHSF1, which contains three 4-3 hydrophobic repeats (LZ1 to LZ3). In unstressed human cells (37 degrees C), hHSF1 appears to be in an inactive, monomeric state that may be maintained through intramolecular interactions stabilized by transient interaction with hsp70. Heat stress (39 to 42 degrees C) disrupts these interactions, and hHSF1 homotrimerizes and acquires heat shock element DNA-binding ability. hHSF1 expressed in Xenopus oocytes also assumes a monomeric, non-DNA-binding state and is converted to a trimeric, DNA-binding form upon exposure of the oocytes to heat shock (35 to 37 degrees C in this organism). Because endogenous HSF DNA-binding activity is low and anti-hHSF1 antibody does not recognize Xenopus HSF, we employed this system for mapping regions in hHSF1 that are required for the maintenance of the monomeric state. The results of mutagenesis analyses strongly suggest that the inactive hHSF1 monomer is stabilized by hydrophobic interactions involving all three leucine zippers which may form a triple-stranded coiled coil. Trimerization may enable the DNA-binding function of hHSF1 by facilitating cooperative binding of monomeric DNA-binding domains to the heat shock element motif. This view is supported by observations that several different LexA DNA-binding domain-hHSF1 chimeras bind to a LexA-binding site in a heat-regulated fashion, that single amino acid replacements disrupting the integrity of hydrophobic repeats render these chimeras constitutively trimeric and DNA binding, and that LexA itself binds stably to DNA only as a dimer but not as a monomer in our assays.

Coordination of Chemical (Trimethylamine Oxide) and Molecular (Heat Shock Protein 70) Chaperone Responses to Heat Stress in Elasmobranch Red Blood Cells

2014 ◽  
Vol 87 (5) ◽  
pp. 652-662 ◽  
Author(s):  
Ashra Kolhatkar ◽  
Cayleih E. Robertson ◽  
Maria E. Thistle ◽  
A. Kurt Gamperl ◽  
Suzanne Currie
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Heat Shock Protein 70 ◽  
Trimethylamine Oxide
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